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Partager le résultat de cette recherche Interroger des sources externesPréparation et caractérisation de complexes dérivés de nouvelles bases de Schiff et de ligand ferrocénique phosphine-carbène N-hétérocyclique / Youssra Doria Lahneche
Titre : Préparation et caractérisation de complexes dérivés de nouvelles bases de Schiff et de ligand ferrocénique phosphine-carbène N-hétérocyclique : Applications biologiques et catalytiques. Type de document : texte imprimé Auteurs : Youssra Doria Lahneche, Auteur ; Meriem Benslimane, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 210 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie: chimie inorganique Phosphine Ferrocène Carbène N-hétérocycle Palladium Suzuki-Miyaura Etude
cinétique Benzimidazole Base de Schiff Mercure Zinc Diffraction de rayons X Calculs
DFT DPPH N-heterocyclic carbene kinetic
study Schiff base Mercury X- ray diffraction DFT calculations البالادیوم فوسفين
دراسة حركية Base de Schiffالزئبق الزنك البنية البلوریة حسابات DFTIndex. décimale : 540 Chimie et sciences connexes Résumé :
In the first part of this thesis, new bidentate ferrocenyl phosphine/N-heterocyclic carbene (NHC) palladium complex rac-5, bearing a functionalized pendant arm for further grafting on a solid surface, has been synthesized in good yields and fully characterized. The molecular structure of proligand rac-4-BF4 and complex rac-5 were obtained by X-ray diffraction on single crystals. Kinetic studies demonstrated the good activity and stability of rac-5 in the Suzuki-Miyaura coupling of aryl bromides with arylboronic acids. The catalyst proved also to be active for the coupling of more challenging, bulky substrates such as 1-bromo-2-methylnaphthalene and naphthylboronic acid at relatively mild temperatures (40–70°C) and low loadings (0.1–0.5 mol%). Experiments carried out with various amounts of water highlighted the role of water on both activity and mechanism. In the second part, a series of new Schiff bases L1-L6 derived from benzimidazole were synthesized and their structures characterized by single-crystal
X-ray diffraction analysis, NMR, IR and UV-Vis spectroscopy. L1, L2, L3, L4 et L6 react with Mercury ions to afford the corresponding complexes 1, 2, 3,4 et 6 in quantitative yield. The ligand L4 was also complexed win zinc ion to afford the corresponding complex 7. The in vitro measurement of the antioxidant activity of some compounds tested (L1, L2, L4, L6, 1, 2 and 7) against the free radical scavenger DPPH at low concentration (100 μg / mL), showed a moderate antioxidant activity (22%-35%), compared to the standard (Asc, 84%). To support experimental data, theoretical calculations including molecular geometry, electronic transitions and vibrational frequencies of the ligand 6 and the (Hg) complex 6 in the ground state were carried out using the global hybrid (B3LYP) density functional. In addition, a qualitative description of excited states and charge transfer character of electronic transitions states were carried out by plotting the Natural Transition Orbitals (NTOs) for main states. Theoretical calculations are in good agreement with experimental values.Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/chimie/LAH7505.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11338 Préparation et caractérisation de complexes dérivés de nouvelles bases de Schiff et de ligand ferrocénique phosphine-carbène N-hétérocyclique : Applications biologiques et catalytiques. [texte imprimé] / Youssra Doria Lahneche, Auteur ; Meriem Benslimane, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 210 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie: chimie inorganique Phosphine Ferrocène Carbène N-hétérocycle Palladium Suzuki-Miyaura Etude
cinétique Benzimidazole Base de Schiff Mercure Zinc Diffraction de rayons X Calculs
DFT DPPH N-heterocyclic carbene kinetic
study Schiff base Mercury X- ray diffraction DFT calculations البالادیوم فوسفين
دراسة حركية Base de Schiffالزئبق الزنك البنية البلوریة حسابات DFTIndex. décimale : 540 Chimie et sciences connexes Résumé :
In the first part of this thesis, new bidentate ferrocenyl phosphine/N-heterocyclic carbene (NHC) palladium complex rac-5, bearing a functionalized pendant arm for further grafting on a solid surface, has been synthesized in good yields and fully characterized. The molecular structure of proligand rac-4-BF4 and complex rac-5 were obtained by X-ray diffraction on single crystals. Kinetic studies demonstrated the good activity and stability of rac-5 in the Suzuki-Miyaura coupling of aryl bromides with arylboronic acids. The catalyst proved also to be active for the coupling of more challenging, bulky substrates such as 1-bromo-2-methylnaphthalene and naphthylboronic acid at relatively mild temperatures (40–70°C) and low loadings (0.1–0.5 mol%). Experiments carried out with various amounts of water highlighted the role of water on both activity and mechanism. In the second part, a series of new Schiff bases L1-L6 derived from benzimidazole were synthesized and their structures characterized by single-crystal
X-ray diffraction analysis, NMR, IR and UV-Vis spectroscopy. L1, L2, L3, L4 et L6 react with Mercury ions to afford the corresponding complexes 1, 2, 3,4 et 6 in quantitative yield. The ligand L4 was also complexed win zinc ion to afford the corresponding complex 7. The in vitro measurement of the antioxidant activity of some compounds tested (L1, L2, L4, L6, 1, 2 and 7) against the free radical scavenger DPPH at low concentration (100 μg / mL), showed a moderate antioxidant activity (22%-35%), compared to the standard (Asc, 84%). To support experimental data, theoretical calculations including molecular geometry, electronic transitions and vibrational frequencies of the ligand 6 and the (Hg) complex 6 in the ground state were carried out using the global hybrid (B3LYP) density functional. In addition, a qualitative description of excited states and charge transfer character of electronic transitions states were carried out by plotting the Natural Transition Orbitals (NTOs) for main states. Theoretical calculations are in good agreement with experimental values.Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/chimie/LAH7505.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11338 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAH/7505 LAH/7505 Thèse Bibliothèque principale Thèses Disponible
Titre : Préparation de nouveaux matériaux à base de (benz)imidazole et bio-activité : synthèse de complexes à base de (benz)imidazole, étude structurale et théorique et préparation d’analogues structuraux de la Tacrine à base de benzimidazole. Type de document : texte imprimé Auteurs : Anfel Benhassine ép Lifa, Auteur ; Ali Belfaitah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 186 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Imidazole Benzimidazole Complexassion Métaux de transition divalents DFT orbitales frontières Tacrine réaction de Friedländer poly-hétérocycles DPPH ABTS activité antimicrobienne Divalent transition metals calculations Frontier orbitals Friedländer's reaction poly-heterocyclic compounds antimicrobial activity إيميدازول بنزيميدازول المعقدات المعادن الانتقالية ثنائية التكافؤ النشاط المضاد للأكسدة التاكرين البنزوبرانوتاكرين النشاط المضاد للميكروبات Index. décimale : 540 Chimie et sciences connexes Résumé : The first part, which is subdivided into two chapters, concerns the synthesis, characterization (IR and UV-Vis, X-ray diffraction, and other elemental analysis) and biological evaluation of divalent transition metal complexes (M = Co , Cu, Cd, Zn, Ni) based on (1-methyl- 1Hbenzo[d]imidazol-2-yl) methanol (L1/Hmbm) (complexes 1-4) and (1-methyl-1H- imidazol-2-yl) methanol (L2/Hmim) (complexes 5-11). In both series of coordination compounds, the ligand (L1 or L2) gives rise to a variety of metal-ligand coordination modes(monodentate or bidentadate) exhibiting tetrahedral or octahedral geometry.The in vitro measurement of the antioxidant activity of the L1 and L2 ligands and their respective metal complexes against the free radical scavenger DPPH at low concentration (100 &g / mL), showed a moderate to significant antioxidant activities, compared to the standard (Asc). The complexes (5-11) and the ligand L2 were submitted to an in vitro evaluation of their antimicrobial activity against five pathogenic strains: animal bacteria:
Gram (+) (S. aureus) and Gram (-) (K pneumonia), a Gram (+) phytopathogenic bacterium (P.
syringae), a yeast (P. caribbica) and a fungus (Trichodermsp), using the disk diffusion method
(agar).The analysis of the results revealed a significant difference in the inhibition profile of the selected strains. The complex 5 and to a lesser extent the complex 6, gave the best results displaying significant inhibitory activities on mostly all pathogenic strains (broad spectrum). In the second part dedicated to the synthesis of structural analogs of Tacrine, a whole series of new poly-heterocyclic compounds (10-14) was prepared from the corresponding 4H-pyrans (5- 9) by an addition-hetero-cyclization reaction of cyclohexanone under the Friedl‰ndler's standard conditions reaction. The in vitro antioxidant tests (DPPH and ABTS) of
benzimidazopyranotacrines, showed that the five compounds have an antioxidant activities, and
the 5-Amino-2-methyl-4-(1-methyl-1H-benzo[d]imidazol-2-yl)-6,7,8,9-tetrahydro-4H-pyrano
[2,3-b]uinolin-3-yl) ethanone (compound 12) is the most efficient, exhibiting a comparable
inhibitory power of free radicals to that of reference compounds (controls). The structure of all the complexes (1-11) was determined by X-ray diffraction, IR, UV and other elemental analysis. For complexes 1-4, theoretical studies using DFT and TD-DFT calculations have been performed and confirmed the experimental results. Benzimidazopyranotacrines (10-14) as structural Tacrine's analogues and their intermediates 4Hpyrans (5-9), were identified by the usual spectroscopic methods (IR, 1H NMR and13C).Diplôme : Doctorat En ligne : ../theses/chimie/LIF7359.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11008 Préparation de nouveaux matériaux à base de (benz)imidazole et bio-activité : synthèse de complexes à base de (benz)imidazole, étude structurale et théorique et préparation d’analogues structuraux de la Tacrine à base de benzimidazole. [texte imprimé] / Anfel Benhassine ép Lifa, Auteur ; Ali Belfaitah, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 186 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Imidazole Benzimidazole Complexassion Métaux de transition divalents DFT orbitales frontières Tacrine réaction de Friedländer poly-hétérocycles DPPH ABTS activité antimicrobienne Divalent transition metals calculations Frontier orbitals Friedländer's reaction poly-heterocyclic compounds antimicrobial activity إيميدازول بنزيميدازول المعقدات المعادن الانتقالية ثنائية التكافؤ النشاط المضاد للأكسدة التاكرين البنزوبرانوتاكرين النشاط المضاد للميكروبات Index. décimale : 540 Chimie et sciences connexes Résumé : The first part, which is subdivided into two chapters, concerns the synthesis, characterization (IR and UV-Vis, X-ray diffraction, and other elemental analysis) and biological evaluation of divalent transition metal complexes (M = Co , Cu, Cd, Zn, Ni) based on (1-methyl- 1Hbenzo[d]imidazol-2-yl) methanol (L1/Hmbm) (complexes 1-4) and (1-methyl-1H- imidazol-2-yl) methanol (L2/Hmim) (complexes 5-11). In both series of coordination compounds, the ligand (L1 or L2) gives rise to a variety of metal-ligand coordination modes(monodentate or bidentadate) exhibiting tetrahedral or octahedral geometry.The in vitro measurement of the antioxidant activity of the L1 and L2 ligands and their respective metal complexes against the free radical scavenger DPPH at low concentration (100 &g / mL), showed a moderate to significant antioxidant activities, compared to the standard (Asc). The complexes (5-11) and the ligand L2 were submitted to an in vitro evaluation of their antimicrobial activity against five pathogenic strains: animal bacteria:
Gram (+) (S. aureus) and Gram (-) (K pneumonia), a Gram (+) phytopathogenic bacterium (P.
syringae), a yeast (P. caribbica) and a fungus (Trichodermsp), using the disk diffusion method
(agar).The analysis of the results revealed a significant difference in the inhibition profile of the selected strains. The complex 5 and to a lesser extent the complex 6, gave the best results displaying significant inhibitory activities on mostly all pathogenic strains (broad spectrum). In the second part dedicated to the synthesis of structural analogs of Tacrine, a whole series of new poly-heterocyclic compounds (10-14) was prepared from the corresponding 4H-pyrans (5- 9) by an addition-hetero-cyclization reaction of cyclohexanone under the Friedl‰ndler's standard conditions reaction. The in vitro antioxidant tests (DPPH and ABTS) of
benzimidazopyranotacrines, showed that the five compounds have an antioxidant activities, and
the 5-Amino-2-methyl-4-(1-methyl-1H-benzo[d]imidazol-2-yl)-6,7,8,9-tetrahydro-4H-pyrano
[2,3-b]uinolin-3-yl) ethanone (compound 12) is the most efficient, exhibiting a comparable
inhibitory power of free radicals to that of reference compounds (controls). The structure of all the complexes (1-11) was determined by X-ray diffraction, IR, UV and other elemental analysis. For complexes 1-4, theoretical studies using DFT and TD-DFT calculations have been performed and confirmed the experimental results. Benzimidazopyranotacrines (10-14) as structural Tacrine's analogues and their intermediates 4Hpyrans (5-9), were identified by the usual spectroscopic methods (IR, 1H NMR and13C).Diplôme : Doctorat En ligne : ../theses/chimie/LIF7359.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11008 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LIF/7359 LIF/7359 Thèse Bibliothèque principale Thèses Disponible
Titre : Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. Type de document : texte imprimé Auteurs : Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2009 Importance : 96 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary.Diplôme : Magistère En ligne : ../theses/biologie/ADJ5396.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1800 Propriétés antioxydantes et activité inhibitrice de la Xanthine oxydase des extraits de la plante médicinale Ajuga iva (L.) Schreber. [texte imprimé] / Moufida Adjadj, Auteur ; Abderrahmane Baghiani, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2009 . - 96 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Flavonoides Stress oxydatif Antioxydants Plantes médicinales Ajuga iva Effet scavenger DPPH Péroxydationlipidique Béta-caroèe/acide linoléique Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for XO inhibitors from Ajuga iva shoot extracts
(AISE) traditionally used in folk medicine in Algeria. XO was purified from fresh bovine
milk with yields of ? ? ? ? ? mg / L, protein / flavine ratio of 5.15 and a single major band, on
SDS-PAGE, of approximately 150 KDa, indicating a high purity of the purified enzyme. The
specific activity of the purified enzyme was of 2055.05 nmol / min / mg protein. The analysis
of methanol (CrE), chloroform (ChE) and ethyl acetate (EaE) extracts, showed that the EaE
has significant amount of polyphenols (35.83 ± 0.69 mg EGA / g dry extract), followed by
ChE and CrE (30.58 ± 0.2 and 17.38 ± 0.29 mg EGA / g dry extract, respectively). The ChE
has the highest flavonoids content (9.64 ± 0.33 mg EQ / g dry extract), followed by EaE and
CrE with 7.72 ± 0.19 et 4.92 ± 0.30 mg EQ / g dry extract, respectively. The ChE showed the
highest inhibitory properties on the XO activity (IC50 = 0.134 ± 0.00032 mg / ml) and on the
cytochrome c reduction (IC50 = 0.23 ± 0.0017 mg / ml), followed by EaE with IC50 of XO
inhibitory activity and an IC50 of the inhibition of cyt c reduction of 0.185 ± 0.0019 and 1.046
± 0.0055 mg /ml, respectively. Finally, CrE had the lowest inhibitory effect on XO activity
and on cyt c reduction with 0.367 ± 0.0041 and 3.34 ± 0.018 mg / ml, respectively. The
antioxidant potential was confirmed with the non enzymatic methods, DPPH assay and β-
carotene / linoleic acid bleaching assay. The highest DPPH radical scavenging activity was
observed with EaE (IC50 = 228.01 ± 2.26 µg / ml), followed by CrE and ChE with IC50 of
415.06 ± 0.59 and 466.33 ± 3.20 µg / ml, respectively. The β-carotene / linoleic acid
bleaching assay revealed that AISE have a very important antioxidant activity. Results
showed that ChE has the highest antioxidant activity (95.64 ± 0.15 %), followed by CrE and
EaE with 87.97 ± 0.10 % and 72.30 ± 0.35 %, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO, free radical
scavenging activity and / or inhibition of lipid peroxidation are necessary.Diplôme : Magistère En ligne : ../theses/biologie/ADJ5396.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1800 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ADJ/5396 ADJ/5396 Thèse Bibliothèque principale Thèses Disponible
Titre : Synthèse et caractérisation d’imines et benzoquinoléines, active biologiquement. Type de document : texte imprimé Auteurs : Nadir Ghichi, Auteur ; Ali Benboudiaf, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 197 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Bases de Schiff RMN DRX monocristal antioxydante DPPH CUPRAC ABTS Shiff bases NMR XRD single crystal antioxidant " قواعد شيف XRDالبلور الحادي مضادات الكسدة Index. décimale : 540 Chimie et sciences connexes Résumé : The principal objective of this thesis is based on the synthesis of two amide-based quinoléine dérivatives, and seven original organic compounds of imine derivatives [Shiff’s base] of bidentate NO type, tridentate NOO and NNO, tetradentate NNOO. These organic compounds were identified by the proton and carbon 13 NMR mthod. The synthesized compounds were prepared according to known methods. The imine derivative structures were determined from the diffraction data measured on single crystal. The results of study of biological activity [antioxidant activity] are as follows :
- The imine derivatives [compound 1, 4 and 7] and the organic compound Q4 showed the best activity with the CUPRAC test. -Compound Q3 showed low trapping activity with the ABTS test. -The organic compounds Q3 and Q4 , and the imine derivative 4 showed a low antiradical activity with the standard DPPH test.Diplôme : Doctorat en sciences En ligne : ../theses/chimie/GHI7358.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11007 Synthèse et caractérisation d’imines et benzoquinoléines, active biologiquement. [texte imprimé] / Nadir Ghichi, Auteur ; Ali Benboudiaf, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 197 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Bases de Schiff RMN DRX monocristal antioxydante DPPH CUPRAC ABTS Shiff bases NMR XRD single crystal antioxidant " قواعد شيف XRDالبلور الحادي مضادات الكسدة Index. décimale : 540 Chimie et sciences connexes Résumé : The principal objective of this thesis is based on the synthesis of two amide-based quinoléine dérivatives, and seven original organic compounds of imine derivatives [Shiff’s base] of bidentate NO type, tridentate NOO and NNO, tetradentate NNOO. These organic compounds were identified by the proton and carbon 13 NMR mthod. The synthesized compounds were prepared according to known methods. The imine derivative structures were determined from the diffraction data measured on single crystal. The results of study of biological activity [antioxidant activity] are as follows :
- The imine derivatives [compound 1, 4 and 7] and the organic compound Q4 showed the best activity with the CUPRAC test. -Compound Q3 showed low trapping activity with the ABTS test. -The organic compounds Q3 and Q4 , and the imine derivative 4 showed a low antiradical activity with the standard DPPH test.Diplôme : Doctorat en sciences En ligne : ../theses/chimie/GHI7358.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11007 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité GHI/7358 GHI/7358 Thèse Bibliothèque principale Thèses Disponible
Titre : Biological activities of Vitis vinifera leaves in cardiovascular diseases induced by hyperhomocysteinemia and on tumoral process. Type de document : texte imprimé Auteurs : Selma Ferhi, Auteur ; sakina Zerizer, Directeur de thèse Mention d'édition : 17/10/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 133 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Biological Sciences and Biotechnology Feuilles de vigne Vitis vinifera HPLC phenols Homocysteine hs-CRP GSH ASE DPPH ERP MCF-7 HepG2 HUVEC Bax/Bcl-2 Grape leaves Vitis vinifera أوراق العنب ( )Vitis viniferaفينولات اليوموسستيين، hs-CRPالجموتاثيون
المختزل GSHمستخرج المذيبات المعجل( )ASEمستقر الجذور الحرة DPPHالكترون رنين مغنطيسي ) (ERP
خلبيا سرطان الثدي ( )MCF-7خلبيا سرطان الكبد ( )HepG2الخمية البطانية من الوريد السري) (HUVEC
الجنيات .Bax/Bcl-Index. décimale : 570 Sciences de la vie. Biologie Résumé : Oxidative stress is defined as an imbalance between productions of free radicals and reactive metabolites. This imbalance leads to damage of important biomolecules and cells, with potential impact on the whole organism. There is currently arenewed interest in phytochemicals as sources of natural antioxidants. Hyperhomocysteinemia is usually defined as an elevation of plasma tHcy. It is an important risk factor for premature cardiovascular disease. As a result, homocysteine metabolism abnormalities are now receiving increasing attention because of their potential role in the pathogenesis of atherosclerosis and other diseases such as venous thrombosis. In this study, we tested in vivo the antioxidant and anti-inflammatory effect of the Grape Leaves (Vitis vinifera) Algerian Variety on the cardiovascular inflammation induced by Hyperhomocysteinemia. The phenolic compounds (Anthocyanins and non anthocyanins) is identified by using HPLC-DAD/ESI-MS, total Homocysteine (t-Hcy) was estimated by competitive solid phase chemiluminescence immunoassay, the plasma hs-CRP is measured by immunoturbidimetric method and the concentration of the GSH measured by spectrophotometric method. In addition, the aorta histology examined in order to confirm the angiotoxic action of homocysteine and the reparative effect of grape leaves (Vitis vinifera) on the aorta. Results showed high levels of phenols, anthocyanins, flavonols and trans-caftaric acid in grape leaves (Vitis vinifera). The plasma hs-CRP and homocysteine levels were elevated significantly (p < 0.05) however the glutathione reduced significantly (p < 0.05) after the administration of L-methionine in high doses to mice. This was associated with the desquamation of endothelium and muscular lysis with transformation of spindle nuclei to oval nuclei; this is due to the angiotoxic action of homocysteine on the aorta. These changes were not observed in mice treated with L-methionine plus the antioxidant and anti-inflammatory extract of grape leaves (Vitis vinifera). So, the study proved the antioxidant and anti-inflammatory effects of the grape leaves (Vitis vinifera) on hyperhomocysteinemia induced inflammatory endothelial damage in cardiovascular diseases. Moreover, in the present thesis, we conducted a study in vitro to analyze the total phenols, anti-oxidative, anti-proliferative properties on MCF-7 and HepG2 Human Cancer Cell Lines and on modulate of Apoptosis-Related Gene Expression of water and ethanol crude extracts obtained by Accelerator Solvent Extractor from grape leaves (Vitis vinifera) grown in Algeria. The results revealed for the first time that accelerator solvent extraction yielded a higher extraction rate of total phenols and antioxidant activity in an extremely short time. The extract obtained from grape leaves (Vitis vinifera) grown in the Medea region (Algeria) exhibited an antiproliferative effect on MCF-7 breast cancer cells and HepG2 hepatocarcinoma cells. Moreover, considering previous reports by other authors and the present results that provides evidence for the modulation of Bax/Bcl2 mRNA levels by leaf extracts, which affects the balance between apoptosis and cell survival, it may be concluded that these extracts could be used as an easily accessible source of natural antioxidants, and as a matrix to prepare drugs counteracting distinctive cancer cells‟ proliferation.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/FER7576.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11414 Biological activities of Vitis vinifera leaves in cardiovascular diseases induced by hyperhomocysteinemia and on tumoral process. [texte imprimé] / Selma Ferhi, Auteur ; sakina Zerizer, Directeur de thèse . - 17/10/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 133 f. ; 30 cm.
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Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Biological Sciences and Biotechnology Feuilles de vigne Vitis vinifera HPLC phenols Homocysteine hs-CRP GSH ASE DPPH ERP MCF-7 HepG2 HUVEC Bax/Bcl-2 Grape leaves Vitis vinifera أوراق العنب ( )Vitis viniferaفينولات اليوموسستيين، hs-CRPالجموتاثيون
المختزل GSHمستخرج المذيبات المعجل( )ASEمستقر الجذور الحرة DPPHالكترون رنين مغنطيسي ) (ERP
خلبيا سرطان الثدي ( )MCF-7خلبيا سرطان الكبد ( )HepG2الخمية البطانية من الوريد السري) (HUVEC
الجنيات .Bax/Bcl-Index. décimale : 570 Sciences de la vie. Biologie Résumé : Oxidative stress is defined as an imbalance between productions of free radicals and reactive metabolites. This imbalance leads to damage of important biomolecules and cells, with potential impact on the whole organism. There is currently arenewed interest in phytochemicals as sources of natural antioxidants. Hyperhomocysteinemia is usually defined as an elevation of plasma tHcy. It is an important risk factor for premature cardiovascular disease. As a result, homocysteine metabolism abnormalities are now receiving increasing attention because of their potential role in the pathogenesis of atherosclerosis and other diseases such as venous thrombosis. In this study, we tested in vivo the antioxidant and anti-inflammatory effect of the Grape Leaves (Vitis vinifera) Algerian Variety on the cardiovascular inflammation induced by Hyperhomocysteinemia. The phenolic compounds (Anthocyanins and non anthocyanins) is identified by using HPLC-DAD/ESI-MS, total Homocysteine (t-Hcy) was estimated by competitive solid phase chemiluminescence immunoassay, the plasma hs-CRP is measured by immunoturbidimetric method and the concentration of the GSH measured by spectrophotometric method. In addition, the aorta histology examined in order to confirm the angiotoxic action of homocysteine and the reparative effect of grape leaves (Vitis vinifera) on the aorta. Results showed high levels of phenols, anthocyanins, flavonols and trans-caftaric acid in grape leaves (Vitis vinifera). The plasma hs-CRP and homocysteine levels were elevated significantly (p < 0.05) however the glutathione reduced significantly (p < 0.05) after the administration of L-methionine in high doses to mice. This was associated with the desquamation of endothelium and muscular lysis with transformation of spindle nuclei to oval nuclei; this is due to the angiotoxic action of homocysteine on the aorta. These changes were not observed in mice treated with L-methionine plus the antioxidant and anti-inflammatory extract of grape leaves (Vitis vinifera). So, the study proved the antioxidant and anti-inflammatory effects of the grape leaves (Vitis vinifera) on hyperhomocysteinemia induced inflammatory endothelial damage in cardiovascular diseases. Moreover, in the present thesis, we conducted a study in vitro to analyze the total phenols, anti-oxidative, anti-proliferative properties on MCF-7 and HepG2 Human Cancer Cell Lines and on modulate of Apoptosis-Related Gene Expression of water and ethanol crude extracts obtained by Accelerator Solvent Extractor from grape leaves (Vitis vinifera) grown in Algeria. The results revealed for the first time that accelerator solvent extraction yielded a higher extraction rate of total phenols and antioxidant activity in an extremely short time. The extract obtained from grape leaves (Vitis vinifera) grown in the Medea region (Algeria) exhibited an antiproliferative effect on MCF-7 breast cancer cells and HepG2 hepatocarcinoma cells. Moreover, considering previous reports by other authors and the present results that provides evidence for the modulation of Bax/Bcl2 mRNA levels by leaf extracts, which affects the balance between apoptosis and cell survival, it may be concluded that these extracts could be used as an easily accessible source of natural antioxidants, and as a matrix to prepare drugs counteracting distinctive cancer cells‟ proliferation.
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/FER7576.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11414 Exemplaires (1)
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