Résultat de la recherche
21 recherche sur le tag
'Oxidative stress' 
Visionner les documents numériques
Affiner la recherche Générer le flux rss de la recherche
Partager le résultat de cette recherche Interroger des sources externesLe rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. / Samira Laib
Titre : Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. Type de document : texte imprimé Auteurs : Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 94 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein لوريد الكادميوم الكلية والإجهاد التأكسدي Thymelaea microphyllaميتالوثيونيين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Diplôme : Doctorat En ligne : ../theses/biologie/LAI7250.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10842 Le rôle de Thymelaea microphylla dans l’amélioration du stress oxydant et du taux de la métallothionéine induits par le cadmium dans le rein chez le rat. [texte imprimé] / Samira Laib, Auteur ; Korichi Lalaoui, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 94 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : chlorure de cadmium rein stress oxydatif Thymelaea microphylla métallothionéine cadmium chloride kidney oxidative stress metallothionein لوريد الكادميوم الكلية والإجهاد التأكسدي Thymelaea microphyllaميتالوثيونيين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present investigation was performed to evaluate the protective effect and the antioxidant power of Thymelaea microphylla against cadmium chloride induced oxidative stress and renal toxicity in Wistar rats. First, antioxidant and radical scavenging effect of Thymelaea microphylla was evaluated in vitro with 2,2- diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals assays. Then, Wistar rats were subjected to oral pretreatment with n-butanolic
extract of Thymelaea microphylla (BETM) (200 mg/kg) against the renal toxicity induced by oral administration of cadmium chloride CdCl2 (5 mg/kg) for 28 days, vitamin E (250 mg/Kg) was used similarly as standard. Efficiency of Thymelaea microphylla against the renal toxicity was evaluated in terms of biochemical estimation of renal function parameters, antioxidant enzymes activities, metallothionein levels and histopathological modifications. Thymelaea
microphylla anti-inflammatory effect was evaluated by carrageenan (1%) induced rat paw edema. Thymelaea microphylla revealed a high scavenging ability of DPPH and OH radicals.
Also, Thymelaea microphylla (200 mg/Kg) pretreatment prevented deteriorative effects caused by cadmium chloride through a protective mechanism that included significant decrease in the levels of Blood Urea Nitrogen, creatinine, gamma-glutamyltranspeptidase (GGT), potassium,sodium, Myeloperoxidase, Nitric Oxide and Thiobarbituric Acid Reactive Species as well as by restoration of antioxidant enzymes in the cytosol, metallothionein levels and
histopathological changes induced by cadmium. Additionally, Thymelaea microphylla revealed an important anti-inflammatory effect reducing carrageenan induced paw edema. This study proved the antioxidant and protective effect of Thymelaea microphylla against cadmium chloride induced oxidative stress and kidney injury.
Diplôme : Doctorat En ligne : ../theses/biologie/LAI7250.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10842 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAI/7250 LAI/7250 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur de la plante Centaurea africana vis-à-vis la neurotoxicité et l’apoptose induits par la roténone chez le rat. Type de document : texte imprimé Auteurs : Sabrina Hadjira, Auteur ; Souad Ameddah, Directeur de thèse Mention d'édition : 19/11/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 114 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Physio-Toxicologie Cellulaire Roténone Centaurea africana Rats Stress oxydatif Activité antioxydante Activité antiinflammatoire Composés phénoliques LC-MS/MS Rotenone Oxidative stress Antioxidant activity Antiinflammatory activity Phenolic compounds جرذان التوترالتاكسدي النشاط المضاد للأكسدة النشاط المضاد للالتهابات عديدات الفينول Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Parkinson's disease (PD) is a chronic neurodegenerative disorder that develops in a slowly progressive manner with no identifiable cause. The aim of this investigation is the study of the biological potential of the n-BuOH and EtOAc fractions extracted from the plant Centaurea africana and elucidate the modulatory effect of the n-BuOH extract in rats (Wistar Albinos) treated with rotenone. In vitro studies (DPPH°, reducing power of iron, inhibition of LPO) have shown that C. africana is endowed with antioxidant properties. Anti-inflammatory studies have shown that nBuOH extract has this effect which is resulting in the inhibition of paw edema induced by carrageenan in rats and decreased activity of MPO and MDA levels in paw tissues. In vivo studies have investigated the modulatory effect of n-BuOH extract of C. africana (200 mg/kg) against biochemical, neurochemical, histopathological and behavioural alterations induced by rotenone (1 mg/kg; ip) in rats. Rotenone causes behavioral and motor deficits and also activates neuroinflammation. The mitochondrial dysfunction induced by ROT is expressed by the decrease in
the activity of complex I of the mitochondrial respiratory chain and by the alteration of the oxidant/antioxidant status of the brain, which activates the intrinsic apoptotic pathway. This study revealed that the n-BuOH extract acts by several mechanisms: neutralization of oxidative stress, modulation of markers of apoptosis (Bcl-2, Bax and caspase 3) and neurochemical markers (dopamine and glutamate), and reduction of neuroinflammation which results in decreased MPO activity and lower levels of NO and TNF. The antioxidant and anti-inflammatory properties of this
plant are probably explained by its richness in polyphenols and flavonoids, which have been revealed by phytochemical screening and LC-MS/MS analysis, and/or by the synergistic interactions of the isolated phenolics.
Diplôme : Doctorat En ligne : ../theses/biologie/HAD7693.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11527 L’effet protecteur de la plante Centaurea africana vis-à-vis la neurotoxicité et l’apoptose induits par la roténone chez le rat. [texte imprimé] / Sabrina Hadjira, Auteur ; Souad Ameddah, Directeur de thèse . - 19/11/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 114 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Physio-Toxicologie Cellulaire Roténone Centaurea africana Rats Stress oxydatif Activité antioxydante Activité antiinflammatoire Composés phénoliques LC-MS/MS Rotenone Oxidative stress Antioxidant activity Antiinflammatory activity Phenolic compounds جرذان التوترالتاكسدي النشاط المضاد للأكسدة النشاط المضاد للالتهابات عديدات الفينول Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Parkinson's disease (PD) is a chronic neurodegenerative disorder that develops in a slowly progressive manner with no identifiable cause. The aim of this investigation is the study of the biological potential of the n-BuOH and EtOAc fractions extracted from the plant Centaurea africana and elucidate the modulatory effect of the n-BuOH extract in rats (Wistar Albinos) treated with rotenone. In vitro studies (DPPH°, reducing power of iron, inhibition of LPO) have shown that C. africana is endowed with antioxidant properties. Anti-inflammatory studies have shown that nBuOH extract has this effect which is resulting in the inhibition of paw edema induced by carrageenan in rats and decreased activity of MPO and MDA levels in paw tissues. In vivo studies have investigated the modulatory effect of n-BuOH extract of C. africana (200 mg/kg) against biochemical, neurochemical, histopathological and behavioural alterations induced by rotenone (1 mg/kg; ip) in rats. Rotenone causes behavioral and motor deficits and also activates neuroinflammation. The mitochondrial dysfunction induced by ROT is expressed by the decrease in
the activity of complex I of the mitochondrial respiratory chain and by the alteration of the oxidant/antioxidant status of the brain, which activates the intrinsic apoptotic pathway. This study revealed that the n-BuOH extract acts by several mechanisms: neutralization of oxidative stress, modulation of markers of apoptosis (Bcl-2, Bax and caspase 3) and neurochemical markers (dopamine and glutamate), and reduction of neuroinflammation which results in decreased MPO activity and lower levels of NO and TNF. The antioxidant and anti-inflammatory properties of this
plant are probably explained by its richness in polyphenols and flavonoids, which have been revealed by phytochemical screening and LC-MS/MS analysis, and/or by the synergistic interactions of the isolated phenolics.
Diplôme : Doctorat En ligne : ../theses/biologie/HAD7693.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11527 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAD/7693 HAD/7693 Thèse Bibliothèque principale Thèses Disponible 
Titre : Enquête sur la consommation des polyphénols auprès d’un échantillon de 200 personnes de la région de Constantine Type de document : texte imprimé Auteurs : Fatima Zohra Becila, Auteur ; Malika Barkat, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 104 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Free radicals oxidative stress antioxidants polyphenols health nutrition radicaux libres apport journalier polyphénols santé alimentation الجذور الحرة الأكسدة مضادات الاكسدة متعدد الفينولات الصحة التغدية Index. décimale : 640 Industrie Alimentaire Résumé : The organism produces free radicals daily. They may result from the action of certain chemical components of our environment (X-rays and UV light) of air pollutants (N, NO2), drugs, smoke, tobacco, alcohol, dietary errors.
Oxidative stress due to free radicals causes tissue damage mainly by oxidation of proteins, DNA or lipids; it is involved in many diseases such as trigger or associated with the development of complications. Antioxidants are compound chemicals that reduce cells’ oxidation. Some are endogenous, while others are obtained from the diet (exogenous). Among them, polyphenols represent a very large proportion of micronutrients provided by our diet. They are becoming increasingly important, particularly caused of their effects of natural antioxidants.
The purpose of the survey is to collect as much information on the consumption of certain foods that contain polyphenols end to know the daily consumption of a sample of 200 subjects taken at random in the Constantine. The questionnaire developed has two main components: a first component summarizes information on the identification of the interviewee, his health, risk factors and sedentary behavior. The second part contains information on foods consumed containing polyphenolic compounds, drawn from the database of the site phenol -explorer (http:// www. Phenol-explorer.eu/contents/total? Food- id = aa1). He also discussed in addition to the quantitative aspect and some processing that may affect the polyphenol composition of foods. From the results obtained, we run down the following key points:
For daily consumption, 76% of subjects surveyed consume vegetables, 48% consume fruits, 39% and 20% drink drinks and herbal teas.
Consumption of vegetables, fruits and beverages depends on the price, while the taste is not the primary factor in daily consumption of herbal teas.
The sick patients consume fewer fresh fruits and vegetables and envelopes, more preserved foods, have more risk factors compared to healthy subjects.
Regarding the consumption of polyphenols majority of respondents (75%) consumption complies with the standard (1g per day) which 2,75% corresponds to sick patients and 92,55% with healthy subjects. Among subjects with the standard lower consumption (25%), 97,43% corresponds to sick patients and 7,45% corresponds to healthy subjects. A weak correlation is found between the consumption of polyphenols and health, A weak correlation is found between the consumption of polyphenols and health.Diplôme : Magistère En ligne : ../theses/agronomie/BEC6482.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9543 Enquête sur la consommation des polyphénols auprès d’un échantillon de 200 personnes de la région de Constantine [texte imprimé] / Fatima Zohra Becila, Auteur ; Malika Barkat, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 104 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Free radicals oxidative stress antioxidants polyphenols health nutrition radicaux libres apport journalier polyphénols santé alimentation الجذور الحرة الأكسدة مضادات الاكسدة متعدد الفينولات الصحة التغدية Index. décimale : 640 Industrie Alimentaire Résumé : The organism produces free radicals daily. They may result from the action of certain chemical components of our environment (X-rays and UV light) of air pollutants (N, NO2), drugs, smoke, tobacco, alcohol, dietary errors.
Oxidative stress due to free radicals causes tissue damage mainly by oxidation of proteins, DNA or lipids; it is involved in many diseases such as trigger or associated with the development of complications. Antioxidants are compound chemicals that reduce cells’ oxidation. Some are endogenous, while others are obtained from the diet (exogenous). Among them, polyphenols represent a very large proportion of micronutrients provided by our diet. They are becoming increasingly important, particularly caused of their effects of natural antioxidants.
The purpose of the survey is to collect as much information on the consumption of certain foods that contain polyphenols end to know the daily consumption of a sample of 200 subjects taken at random in the Constantine. The questionnaire developed has two main components: a first component summarizes information on the identification of the interviewee, his health, risk factors and sedentary behavior. The second part contains information on foods consumed containing polyphenolic compounds, drawn from the database of the site phenol -explorer (http:// www. Phenol-explorer.eu/contents/total? Food- id = aa1). He also discussed in addition to the quantitative aspect and some processing that may affect the polyphenol composition of foods. From the results obtained, we run down the following key points:
For daily consumption, 76% of subjects surveyed consume vegetables, 48% consume fruits, 39% and 20% drink drinks and herbal teas.
Consumption of vegetables, fruits and beverages depends on the price, while the taste is not the primary factor in daily consumption of herbal teas.
The sick patients consume fewer fresh fruits and vegetables and envelopes, more preserved foods, have more risk factors compared to healthy subjects.
Regarding the consumption of polyphenols majority of respondents (75%) consumption complies with the standard (1g per day) which 2,75% corresponds to sick patients and 92,55% with healthy subjects. Among subjects with the standard lower consumption (25%), 97,43% corresponds to sick patients and 7,45% corresponds to healthy subjects. A weak correlation is found between the consumption of polyphenols and health, A weak correlation is found between the consumption of polyphenols and health.Diplôme : Magistère En ligne : ../theses/agronomie/BEC6482.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9543 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEC/6482 BEC/6482 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux : Exemple des Triticum et Hordeum. Type de document : texte imprimé Auteurs : Sarra Merabta, Auteur ; Mostefa Benlaribi, Directeur de thèse Mention d'édition : 18/12/2019 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 164 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Biotechnologies, Biologie et Environnement Biotechnologies, Biologie et Environnement:Les bases Biologiques de la Production et Biodiversité Végétale Blé (Triticum) orge(Hordeum) stress hydrique Proline chlorophylle Stress oxydatif enzymes Wheat (Triticum) Barley (Hordeum) hydrous stress Chlorophyll Oxidative stress القمح ( )Triticumالشعير ( )Hordeumالاجهاد المائي البرولين الكلوروفيل الاجهاد
التأكسد الانزيماتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The objective of this work consists in evaluating the effect of a hydrous deficit created using a restriction of irrigation for a period of twenty (20) days on two durum wheat genotypes (Triticum durum Desf.): Gamgoum Rekham ( GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence Aurore 8193 (FA) and Mexipak and two genotypes of barley (Hordeum vulgare L.): Manel and Saida 183 and to follow the impact of this controlled stress after 24 hours (APR1) and one week (APR2), following the return of watering. A first experiment is carried out in order to compare the relation between Proline and chlorophyll, the application of prolonged hydric stress causes an accumulation of the content of proline in the leaves of the two kinds tested compared to that of their witnesses. However, this accumulation is in favor of the Triticum kind beside the Hordeum kind with a remarkable
diversity within the different varieties. As for the total chlorophyll composition of the organs studied, it evolves in a sawtooth pattern that does not lead to any indicative trend during the various treatments. After rehydration, there is a progressive decrease in proline content (APR1 and APR2), particularly at the two barley genotypes, and any change concerning the chlorophyll content. One second experimentation is carried out on the same genotypes with the same hydric treatments but this time to determine the influence of the oxydative stress produced by the dryness on the antioxydant metabolism. We were particularly interested in the activity of the superoxyde dismutase (SOD), malonedialdéhydes (MDA) content, catalase (CAT), peroxidases content (POD), as well as the ascorbate peroxidases (APX), and the content of proteins, aiming to characterize varieties according to their defense mechanisms against the effects of free radicals induced by water stress more precisely during the most critical stage and more affected by this Lack (heading stage) and to evaluate the impact of the oxydative
defensive system (Indicators of oxidation and antioxidant enzymes activities) on the synthesis and the degradation of the proline in order to identify the elements of the signaling pathways involved, and to evaluate the effect of this stress on the synthesis of this compound since the precursor molecules and its fate after the return of watering.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/MER7611.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11447 L’accumulation de la proline sous déficit hydrique, synthèse et devenir après retour de l’arrosage chez les végétaux : Exemple des Triticum et Hordeum. [texte imprimé] / Sarra Merabta, Auteur ; Mostefa Benlaribi, Directeur de thèse . - 18/12/2019 . - جامعة الإخوة منتوري قسنطينة, 2019 . - 164 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale:Biotechnologies, Biologie et Environnement Biotechnologies, Biologie et Environnement:Les bases Biologiques de la Production et Biodiversité Végétale Blé (Triticum) orge(Hordeum) stress hydrique Proline chlorophylle Stress oxydatif enzymes Wheat (Triticum) Barley (Hordeum) hydrous stress Chlorophyll Oxidative stress القمح ( )Triticumالشعير ( )Hordeumالاجهاد المائي البرولين الكلوروفيل الاجهاد
التأكسد الانزيماتIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
The objective of this work consists in evaluating the effect of a hydrous deficit created using a restriction of irrigation for a period of twenty (20) days on two durum wheat genotypes (Triticum durum Desf.): Gamgoum Rekham ( GGR) and Haurani; two genotypes of common wheat (Triticum aestivum L.): Florence Aurore 8193 (FA) and Mexipak and two genotypes of barley (Hordeum vulgare L.): Manel and Saida 183 and to follow the impact of this controlled stress after 24 hours (APR1) and one week (APR2), following the return of watering. A first experiment is carried out in order to compare the relation between Proline and chlorophyll, the application of prolonged hydric stress causes an accumulation of the content of proline in the leaves of the two kinds tested compared to that of their witnesses. However, this accumulation is in favor of the Triticum kind beside the Hordeum kind with a remarkable
diversity within the different varieties. As for the total chlorophyll composition of the organs studied, it evolves in a sawtooth pattern that does not lead to any indicative trend during the various treatments. After rehydration, there is a progressive decrease in proline content (APR1 and APR2), particularly at the two barley genotypes, and any change concerning the chlorophyll content. One second experimentation is carried out on the same genotypes with the same hydric treatments but this time to determine the influence of the oxydative stress produced by the dryness on the antioxydant metabolism. We were particularly interested in the activity of the superoxyde dismutase (SOD), malonedialdéhydes (MDA) content, catalase (CAT), peroxidases content (POD), as well as the ascorbate peroxidases (APX), and the content of proteins, aiming to characterize varieties according to their defense mechanisms against the effects of free radicals induced by water stress more precisely during the most critical stage and more affected by this Lack (heading stage) and to evaluate the impact of the oxydative
defensive system (Indicators of oxidation and antioxidant enzymes activities) on the synthesis and the degradation of the proline in order to identify the elements of the signaling pathways involved, and to evaluate the effect of this stress on the synthesis of this compound since the precursor molecules and its fate after the return of watering.Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/MER7611.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11447 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MER/7611 MER/7611 Thèse Bibliothèque principale Thèses Disponible
Titre : Neurodegeneration, inflammation, oxidative stress and behavioral deficit following bilateral short term adrenalectomy in the nervous system of albino Wistar rats Type de document : texte imprimé Auteurs : Naserddine Hamadi, Auteur ; Fatima Khelifi Touhami, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 194 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : surrénalectomie Hippocampe Neuroinflammation neurodégénérescence stress oxydatif Adrenalectomy Hippocampus Neurodegeneration Oxidative stress استئصال الغدة الكظرية الحصين التهابات عصبية تلف الخلايا العصبية الاجهاد التأكسدي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM7230.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10797 Neurodegeneration, inflammation, oxidative stress and behavioral deficit following bilateral short term adrenalectomy in the nervous system of albino Wistar rats [texte imprimé] / Naserddine Hamadi, Auteur ; Fatima Khelifi Touhami, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 194 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : surrénalectomie Hippocampe Neuroinflammation neurodégénérescence stress oxydatif Adrenalectomy Hippocampus Neurodegeneration Oxidative stress استئصال الغدة الكظرية الحصين التهابات عصبية تلف الخلايا العصبية الاجهاد التأكسدي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Bilateral adrenalectomy has been shown to damage the hippocampal neurons. Although the effects of long-term adrenalectomy have
been studied extensively there are few publications on the effects of short-term adrenalectomy. In the present study we aimed to investigate
the effects of short-term bilateral adrenalectomy on the levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α; the response of
microglia and astrocytes to neuronal cell death animal behavior as well as oxidative stress markers GSH, SOD and MDA over the course of
time (4h, 24h, 3days, 1week and 2weeks) in the hippocampus of Wistar rats.
Our results showed a transient significant elevation of pro-inflammatory cytokines IL-1β and IL-6 from four hours to three days in
the adrenalectomized compared to sham operated rats. After one week, the elevation of both cytokines returns to the sham levels.
Surprisingly, TNF-α levels were significantly elevated at four hours only in adrenalectomized compared to sham operated rats. The
occurrence of neuronal cell death in the hippocampus following adrenalectomy was confirmed by Fluoro-Jade B staining. Our results showed
a time dependent increase in degenerated neurons in the dorsal blade of the dentate gyrus from three days to two weeks after adrenalectomy.
Our results revealed an early activation of microglia on day three whereas activation of astroglia in the hippocampus was observed at one
week postoperatively. A progression of microglia and astroglia activation all over the dentate gyrus and their appearance for the first time in
CA3 of adrenalectomized rats hippocampi compared to sham operated was seen after two weeks of surgery. Quantitative analysis revealed a
significant increase in the number of microglia (3, 7 and 14 days) and astrocytes (7 and 14 days) of ADX compared to sham operated rats. Our
study revealed no major signs of oxidative stress until two weeks after adrenalectomy when a significant decrease of GSH levels and SOD
activity as well as an increase in MDA levels were found in adrenalectomized compared to sham rats. In the current study we used passive
avoidance test to evaluate the cognitive functions of the ADX rats, we have found that the removal of the adrenal gland caused a
behavioral deficit in the adrenalectomized rats compared to the sham over the time (3, 7 and 14 days).
Our study showed an early increase in the pro-inflammatory cytokines followed by neurodegeneration and activation of glial
cells as well as oxidative stress. Taking these findings together it could be speculated that the early inflammatory components might
contribute to the initiation of the biological cascade responsible for subsequent neuronal death in the current neurodegenerative animal model.
These findings suggest that inflammatory mechanisms precede neurodegeneration and glial activation
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM7230.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10797 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAM/7230 HAM/7230 Thèse Bibliothèque principale Thèses Disponible
Permalink
Permalink
PermalinkPermalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
Permalink
