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Partager le résultat de cette recherche Interroger des sources externesCaractérisation de la composition en microconstituants des margines issues de la production oléicole et utilisabilité comme complément dans la ration chez la vache laitière / Moufida Aggoun-Arhab
Titre : Caractérisation de la composition en microconstituants des margines issues de la production oléicole et utilisabilité comme complément dans la ration chez la vache laitière Type de document : texte imprimé Auteurs : Moufida Aggoun-Arhab, Auteur ; Malika Barkat, Directeur de thèse ; Benoit Graulet, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 178 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Composés phénoliques caroténoïdes tocophérols margines variétés d’olive procédés d’extraction composition chimique vache laitière fermentation ruminale Phenolic compounds carotenoids tocopherols olive wastewater olive variety olive milling chemical composition dairy cow ruminal fermentation ???????? ????????? ??????????? ????? ????? ??????? ?????
??????? ??????? ??????? ????????? ???? ???? ? ?????? ?? ?????Index. décimale : 640 Industrie Alimentaire Résumé : Olive oil production yields a considerable amount of wastewater, a powerful pollutant
that is currently discarded but could be considered as a potential source of valuable natural products due to its content in phenolic compounds and other natural antioxidants. The aim of this work was to valorize this by-product by ruminants. Thus, the proposed strategy was to distribute olive mill wastewater (OMWW) to dairy cows in production. This large-scale recovery required two prior steps: i) explore the variability in OMWW composition from Algerian olive oil mills considering extraction processes (traditional discontinuous press vs 3-phases centrifugal system)
and olive varieties (Azerradj, Sigoise, Chemlal) and, ii) estimate its in vitro degradability in presence of ruminale microbiota in batch systems. Whereas pH, dry or organic matter content didn't vary, there was a significant difference in ash content according to extraction process and olive variety. Carotenoid content was 2.2-fold higher with 3-phases than with press systems whereas tocopherol content was not significantly different. Among the phenolic compounds quantified, tyrosol was usually the most abundant whereas oleuropein concentrations were highly variable. Differences in phenolic compound concentrations were more pronounced between olive varieties than between processes.
The anaerobic biodegradation of OMWW reveals their extensive use by the rumen microbiota. Compared to vetch hay oats, they produce a small amount of gas. In addition, in vitro fermentation generates a low volume of methane. This allows OMWW to improve the efficiency of ruminal microbial flora in the biomass production and inhibition of methane production, a major source of energy loss for the animal. This result allows us to recommend their use as a food additive in the diet of ruminants
The distribution of OMWW in the diet of dairy cows has allowed us to define the
conditions for presentation of OMWW to dairy cows (shape, adaptation, and intake level) and their acceptability. Our results showed that the level of quantitative output (milk production) and fine composition of milk (vitamin A, E and phenolic compounds) did not seem to be affected significantly. The lack of significant differences between groups of animals is due to the fact that the OMWW distributed to the cows were not particularly rich in micro-components compared to forage.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/AGG6913.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10265 Caractérisation de la composition en microconstituants des margines issues de la production oléicole et utilisabilité comme complément dans la ration chez la vache laitière [texte imprimé] / Moufida Aggoun-Arhab, Auteur ; Malika Barkat, Directeur de thèse ; Benoit Graulet, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 178 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Composés phénoliques caroténoïdes tocophérols margines variétés d’olive procédés d’extraction composition chimique vache laitière fermentation ruminale Phenolic compounds carotenoids tocopherols olive wastewater olive variety olive milling chemical composition dairy cow ruminal fermentation ???????? ????????? ??????????? ????? ????? ??????? ?????
??????? ??????? ??????? ????????? ???? ???? ? ?????? ?? ?????Index. décimale : 640 Industrie Alimentaire Résumé : Olive oil production yields a considerable amount of wastewater, a powerful pollutant
that is currently discarded but could be considered as a potential source of valuable natural products due to its content in phenolic compounds and other natural antioxidants. The aim of this work was to valorize this by-product by ruminants. Thus, the proposed strategy was to distribute olive mill wastewater (OMWW) to dairy cows in production. This large-scale recovery required two prior steps: i) explore the variability in OMWW composition from Algerian olive oil mills considering extraction processes (traditional discontinuous press vs 3-phases centrifugal system)
and olive varieties (Azerradj, Sigoise, Chemlal) and, ii) estimate its in vitro degradability in presence of ruminale microbiota in batch systems. Whereas pH, dry or organic matter content didn't vary, there was a significant difference in ash content according to extraction process and olive variety. Carotenoid content was 2.2-fold higher with 3-phases than with press systems whereas tocopherol content was not significantly different. Among the phenolic compounds quantified, tyrosol was usually the most abundant whereas oleuropein concentrations were highly variable. Differences in phenolic compound concentrations were more pronounced between olive varieties than between processes.
The anaerobic biodegradation of OMWW reveals their extensive use by the rumen microbiota. Compared to vetch hay oats, they produce a small amount of gas. In addition, in vitro fermentation generates a low volume of methane. This allows OMWW to improve the efficiency of ruminal microbial flora in the biomass production and inhibition of methane production, a major source of energy loss for the animal. This result allows us to recommend their use as a food additive in the diet of ruminants
The distribution of OMWW in the diet of dairy cows has allowed us to define the
conditions for presentation of OMWW to dairy cows (shape, adaptation, and intake level) and their acceptability. Our results showed that the level of quantitative output (milk production) and fine composition of milk (vitamin A, E and phenolic compounds) did not seem to be affected significantly. The lack of significant differences between groups of animals is due to the fact that the OMWW distributed to the cows were not particularly rich in micro-components compared to forage.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/AGG6913.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10265 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AGG/6913 AGG/6913 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et évaluation des activités biologiques de la plante Daucus muricatus (Apiaceae) Type de document : texte imprimé Auteurs : Amira Noui, Auteur ; Salah Akkal, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 85 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Apiaceae Daucus muricatus LC-ESI-MS/MS Composés phénoliques Activité antioxydante phenolic compounds antioxidant activity ??????? ??????? ????????????? ???????? ??????? ??????? Index. décimale : 540 Chimie et sciences connexes Résumé : This work is dedicate to the phytochemical study of Daucus muricatus species (Apiaceae), it was aimed at investigating the phytochemical components of butanolic extract using HPLC-DAD-ESI-MS/MS. The proposed method provided tentative identification of 25 phenolic compounds, including, 13 hydroxycinnamic acid derivatives, 5 hydroxybenzoïc acid derivatives,8 flavonoïds and other polar
compounds as quinic acid. Evaluation of the antioxidant activities by using four complementary tests (DPPH, ABTS, CUPRAC and carotene Bleanching) showed that both extracts exhibited importatnt antioxidant activities.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/NOU7349.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10999 Etude phytochimique et évaluation des activités biologiques de la plante Daucus muricatus (Apiaceae) [texte imprimé] / Amira Noui, Auteur ; Salah Akkal, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 85 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Apiaceae Daucus muricatus LC-ESI-MS/MS Composés phénoliques Activité antioxydante phenolic compounds antioxidant activity ??????? ??????? ????????????? ???????? ??????? ??????? Index. décimale : 540 Chimie et sciences connexes Résumé : This work is dedicate to the phytochemical study of Daucus muricatus species (Apiaceae), it was aimed at investigating the phytochemical components of butanolic extract using HPLC-DAD-ESI-MS/MS. The proposed method provided tentative identification of 25 phenolic compounds, including, 13 hydroxycinnamic acid derivatives, 5 hydroxybenzoïc acid derivatives,8 flavonoïds and other polar
compounds as quinic acid. Evaluation of the antioxidant activities by using four complementary tests (DPPH, ABTS, CUPRAC and carotene Bleanching) showed that both extracts exhibited importatnt antioxidant activities.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/NOU7349.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10999 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité NOU/7349 NOU/7349 Thèse Bibliothèque principale Thèses Disponible
Titre : Evaluation des propriétés biopharmacologiques, standardisation chimique et valorisation des agroressources fonctionnelles cas de Vitis vinifera, Punica granatum, Citrus aurantium et Opuntia ficus-indica. Type de document : texte imprimé Auteurs : Nadia Zeghad ep Bououden, Auteur ; Abdelmalik Belkhiri, Directeur de thèse ; Kristiaan Demeyer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 149 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles. Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Vitis vinifera Punica granatum Citrus aurantium Opuntia ficus-indica composés phénoliques activités biopharmacologiques phenolic compounds biopharmacological activities ????? Vitis vinifera ?????? Punica granatum ???????? ???? Citrus aurantium ????? ?????? Opuntia ficus-indica ??????? ????????????????? ???????? ????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present work was based on the valorization of agroresources of four fruits grown locally
in Algeria; Vitis vinifera, Punica granatum, Citrus aurantium and Opuntia ficus-indica by the
determination of their molecular characterizations and biological activities.
The hydroalcoholic extracts of the selected fruits were evaluated for their antioxidant,
analgesic, healing and antihyperlipidemic activities as well as their toxic potential.
A chromatographic analysis by HPLC-DAD-ESI/MS was performed and supplemented by a
quantitative evaluation of the total phenols, flavonoïds, flavonols, condensed and
hydrolysable tannins. The results of the quantitative analysis show that the levels of the of
Vitis vinifera fruit extract on phenolic compounds are significantly higher than those of other
fruits. The results of the HPLC-DAD-ESI/MS analysis revealed a chromatographic profile
dominated by a bargaptene (coumarin) for the extract of Punica granatum and by the phenolic
acids and flavonoïds for the other extracts.
The antioxidant activity (DPPH•, ABTS•+, FRAP, ORAC, TBARS, NO•) evaluated in vitro
showed that the antioxidant potential of the Vitis vinifera extract is the most important
compared to the other extracts. The Punica granatum extract has also a potential antioxidant
activity but it remains less important than that of the previous species.
The Vitis vinifera and Punica granatum extracts showed an analgesic effect on the three tests
(torsion, hot plate and tail immersion), of which the potential to reduce the pain was
statistically comparable in both extracts and was most evident in the torsion test. On the other
hand, the results of the evaluation of the healing activity in the rabbits showed that the Punica
granatum extract and at lesser degree that of Vitis vinifera accelerate the process of healing
of excisional wounds and burns compared to the control, and was even superior to that of
commercial healing cream (Cicatryl®). The results of the antihyperlipidemic activity showed
that the Vitis vinifera and Punica granatum extracts normalized the lipidemic profile in mice
made hyperlipidemic compared to the control. Finally, the results of the acute toxicity in mice
demonstrated that the Vitis vinifera and Punica granatum extracts are free of any signs of
toxicity at the maximum dose prescribed.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ZEG7208.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10755 Evaluation des propriétés biopharmacologiques, standardisation chimique et valorisation des agroressources fonctionnelles cas de Vitis vinifera, Punica granatum, Citrus aurantium et Opuntia ficus-indica. [texte imprimé] / Nadia Zeghad ep Bououden, Auteur ; Abdelmalik Belkhiri, Directeur de thèse ; Kristiaan Demeyer, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 149 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Vitis vinifera Punica granatum Citrus aurantium Opuntia ficus-indica composés phénoliques activités biopharmacologiques phenolic compounds biopharmacological activities ????? Vitis vinifera ?????? Punica granatum ???????? ???? Citrus aurantium ????? ?????? Opuntia ficus-indica ??????? ????????????????? ???????? ????????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present work was based on the valorization of agroresources of four fruits grown locally
in Algeria; Vitis vinifera, Punica granatum, Citrus aurantium and Opuntia ficus-indica by the
determination of their molecular characterizations and biological activities.
The hydroalcoholic extracts of the selected fruits were evaluated for their antioxidant,
analgesic, healing and antihyperlipidemic activities as well as their toxic potential.
A chromatographic analysis by HPLC-DAD-ESI/MS was performed and supplemented by a
quantitative evaluation of the total phenols, flavonoïds, flavonols, condensed and
hydrolysable tannins. The results of the quantitative analysis show that the levels of the of
Vitis vinifera fruit extract on phenolic compounds are significantly higher than those of other
fruits. The results of the HPLC-DAD-ESI/MS analysis revealed a chromatographic profile
dominated by a bargaptene (coumarin) for the extract of Punica granatum and by the phenolic
acids and flavonoïds for the other extracts.
The antioxidant activity (DPPH•, ABTS•+, FRAP, ORAC, TBARS, NO•) evaluated in vitro
showed that the antioxidant potential of the Vitis vinifera extract is the most important
compared to the other extracts. The Punica granatum extract has also a potential antioxidant
activity but it remains less important than that of the previous species.
The Vitis vinifera and Punica granatum extracts showed an analgesic effect on the three tests
(torsion, hot plate and tail immersion), of which the potential to reduce the pain was
statistically comparable in both extracts and was most evident in the torsion test. On the other
hand, the results of the evaluation of the healing activity in the rabbits showed that the Punica
granatum extract and at lesser degree that of Vitis vinifera accelerate the process of healing
of excisional wounds and burns compared to the control, and was even superior to that of
commercial healing cream (Cicatryl®). The results of the antihyperlipidemic activity showed
that the Vitis vinifera and Punica granatum extracts normalized the lipidemic profile in mice
made hyperlipidemic compared to the control. Finally, the results of the acute toxicity in mice
demonstrated that the Vitis vinifera and Punica granatum extracts are free of any signs of
toxicity at the maximum dose prescribed.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ZEG7208.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10755 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ZEG/7208 ZEG/7208 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo Type de document : texte imprimé Auteurs : Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 140 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds ?????? ???????? ????? ?????? ??????? ???????? ??????? ???????? ????????? ??????? ??????? ??????Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10833 L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo [texte imprimé] / Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 140 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds ?????? ???????? ????? ?????? ??????? ???????? ??????? ???????? ????????? ??????? ??????? ??????Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10833 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAH/7238 LAH/7238 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur des extraits des plantes Helianthemum confertum et Helianthemum ruficomum vis-à-vis de la toxicité induite par la Doxorubicine : Etude in vitro et in vivo Type de document : texte imprimé Auteurs : Radja Djebbari, Auteur ; Djamila Zama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 122 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles.
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Helianthemum confertum Helianthemum ruficomum stress oxydative activités anti oxydants Doxorubicine extraits des plantes peroxydation lipidique composes phénoliques oxidative stress Doxorubicin Antioxidant activities lipid peroxidation Plant extract Phenolic compounds Doxorubicine???????? ??????? ????????? ?????? ?????? ??????? ?????????????? ??????? ??????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aims to investigate the protective and antioxidant effects of n-butanol extracts of H. confertum and H. ruficomum plants in experimental rats. The n-butanol extract of those plants was evaluated in Wistar Albinos rats for its protective potentials against doxorubicin induced acute toxicity. The effect of n-butanol extracts of H. confertum and H. ruficomum at a dose of (50mg/kg and 100mg/kg) was studied on DOX-induced hepatic, kidney and heart damage. One dose of doxorubicin (10mg/kg and 15mg/kg) was injected intraperitoneal in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, LDH, urea, creatinine, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver, kidney and heart damage. It was found that combined H.
confertum and H. ruficomum (50 and 100mg/kg) extract and DOX (10 and15mg/kg) treatment decreased significantly TBARS level and biochemical parameters. In contract increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the DOX-group. Results of in vivo experiments showed that the n-butanol extracts of H. confertum and H. ruficomum inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity
and maintained the levels of antioxidants enzymes. The protective effect of vitamin E was also studied against doxorubicin-induced acute toxicity at (15mg/kg), and it was found that combined vitamin E (100mg/kg) and DOX (15mg/kg) treatment decreased significantly TBARS level and increased antioxidants activities.
Also, histological study was determined in pretreatment with n-butanol extract of H. confertum and doxorubicin induced acute toxicity at (10mg/kg), and it was found that combined H. confertum n-butanol extract at (50 and 100mg/kg) decreased necrotic cells in hepatic tissue. The antioxidant in vitro tests (DPPH scavenging, inhibition of LPO and anti-cancer activity) of n-butanol extracts of H. confertum and H. ruficomum indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols.Diplôme : Doctorat En ligne : ../theses/biologie/DJE7167.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10590 L’effet protecteur des extraits des plantes Helianthemum confertum et Helianthemum ruficomum vis-à-vis de la toxicité induite par la Doxorubicine : Etude in vitro et in vivo [texte imprimé] / Radja Djebbari, Auteur ; Djamila Zama, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 122 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Helianthemum confertum Helianthemum ruficomum stress oxydative activités anti oxydants Doxorubicine extraits des plantes peroxydation lipidique composes phénoliques oxidative stress Doxorubicin Antioxidant activities lipid peroxidation Plant extract Phenolic compounds Doxorubicine???????? ??????? ????????? ?????? ?????? ??????? ?????????????? ??????? ??????? ?????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aims to investigate the protective and antioxidant effects of n-butanol extracts of H. confertum and H. ruficomum plants in experimental rats. The n-butanol extract of those plants was evaluated in Wistar Albinos rats for its protective potentials against doxorubicin induced acute toxicity. The effect of n-butanol extracts of H. confertum and H. ruficomum at a dose of (50mg/kg and 100mg/kg) was studied on DOX-induced hepatic, kidney and heart damage. One dose of doxorubicin (10mg/kg and 15mg/kg) was injected intraperitoneal in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, LDH, urea, creatinine, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver, kidney and heart damage. It was found that combined H.
confertum and H. ruficomum (50 and 100mg/kg) extract and DOX (10 and15mg/kg) treatment decreased significantly TBARS level and biochemical parameters. In contract increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the DOX-group. Results of in vivo experiments showed that the n-butanol extracts of H. confertum and H. ruficomum inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity
and maintained the levels of antioxidants enzymes. The protective effect of vitamin E was also studied against doxorubicin-induced acute toxicity at (15mg/kg), and it was found that combined vitamin E (100mg/kg) and DOX (15mg/kg) treatment decreased significantly TBARS level and increased antioxidants activities.
Also, histological study was determined in pretreatment with n-butanol extract of H. confertum and doxorubicin induced acute toxicity at (10mg/kg), and it was found that combined H. confertum n-butanol extract at (50 and 100mg/kg) decreased necrotic cells in hepatic tissue. The antioxidant in vitro tests (DPPH scavenging, inhibition of LPO and anti-cancer activity) of n-butanol extracts of H. confertum and H. ruficomum indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols.Diplôme : Doctorat En ligne : ../theses/biologie/DJE7167.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10590 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJE/7167 DJE/7167 Thèse Bibliothèque principale Thèses Disponible
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