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Partager le résultat de cette recherche Interroger des sources externesL’effet protecteur de la plante Centaurea africana vis-à-vis la neurotoxicité et l’apoptose induits par la roténone chez le rat. / Sabrina Hadjira
Titre : L’effet protecteur de la plante Centaurea africana vis-à-vis la neurotoxicité et l’apoptose induits par la roténone chez le rat. Type de document : texte imprimé Auteurs : Sabrina Hadjira, Auteur ; Souad Ameddah, Directeur de thèse Mention d'édition : 19/11/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 114 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Physio-Toxicologie Cellulaire Roténone Centaurea africana Rats Stress oxydatif Activité antioxydante Activité antiinflammatoire Composés phénoliques LC-MS/MS Rotenone Oxidative stress Antioxidant activity Antiinflammatory activity Phenolic compounds جرذان التوترالتاكسدي النشاط المضاد للأكسدة النشاط المضاد للالتهابات عديدات الفينول Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Parkinson's disease (PD) is a chronic neurodegenerative disorder that develops in a slowly progressive manner with no identifiable cause. The aim of this investigation is the study of the biological potential of the n-BuOH and EtOAc fractions extracted from the plant Centaurea africana and elucidate the modulatory effect of the n-BuOH extract in rats (Wistar Albinos) treated with rotenone. In vitro studies (DPPH°, reducing power of iron, inhibition of LPO) have shown that C. africana is endowed with antioxidant properties. Anti-inflammatory studies have shown that nBuOH extract has this effect which is resulting in the inhibition of paw edema induced by carrageenan in rats and decreased activity of MPO and MDA levels in paw tissues. In vivo studies have investigated the modulatory effect of n-BuOH extract of C. africana (200 mg/kg) against biochemical, neurochemical, histopathological and behavioural alterations induced by rotenone (1 mg/kg; ip) in rats. Rotenone causes behavioral and motor deficits and also activates neuroinflammation. The mitochondrial dysfunction induced by ROT is expressed by the decrease in
the activity of complex I of the mitochondrial respiratory chain and by the alteration of the oxidant/antioxidant status of the brain, which activates the intrinsic apoptotic pathway. This study revealed that the n-BuOH extract acts by several mechanisms: neutralization of oxidative stress, modulation of markers of apoptosis (Bcl-2, Bax and caspase 3) and neurochemical markers (dopamine and glutamate), and reduction of neuroinflammation which results in decreased MPO activity and lower levels of NO and TNF. The antioxidant and anti-inflammatory properties of this
plant are probably explained by its richness in polyphenols and flavonoids, which have been revealed by phytochemical screening and LC-MS/MS analysis, and/or by the synergistic interactions of the isolated phenolics.
Diplôme : Doctorat En ligne : ../theses/biologie/HAD7693.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11527 L’effet protecteur de la plante Centaurea africana vis-à-vis la neurotoxicité et l’apoptose induits par la roténone chez le rat. [texte imprimé] / Sabrina Hadjira, Auteur ; Souad Ameddah, Directeur de thèse . - 19/11/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 114 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Physio-Toxicologie Cellulaire Roténone Centaurea africana Rats Stress oxydatif Activité antioxydante Activité antiinflammatoire Composés phénoliques LC-MS/MS Rotenone Oxidative stress Antioxidant activity Antiinflammatory activity Phenolic compounds جرذان التوترالتاكسدي النشاط المضاد للأكسدة النشاط المضاد للالتهابات عديدات الفينول Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Parkinson's disease (PD) is a chronic neurodegenerative disorder that develops in a slowly progressive manner with no identifiable cause. The aim of this investigation is the study of the biological potential of the n-BuOH and EtOAc fractions extracted from the plant Centaurea africana and elucidate the modulatory effect of the n-BuOH extract in rats (Wistar Albinos) treated with rotenone. In vitro studies (DPPH°, reducing power of iron, inhibition of LPO) have shown that C. africana is endowed with antioxidant properties. Anti-inflammatory studies have shown that nBuOH extract has this effect which is resulting in the inhibition of paw edema induced by carrageenan in rats and decreased activity of MPO and MDA levels in paw tissues. In vivo studies have investigated the modulatory effect of n-BuOH extract of C. africana (200 mg/kg) against biochemical, neurochemical, histopathological and behavioural alterations induced by rotenone (1 mg/kg; ip) in rats. Rotenone causes behavioral and motor deficits and also activates neuroinflammation. The mitochondrial dysfunction induced by ROT is expressed by the decrease in
the activity of complex I of the mitochondrial respiratory chain and by the alteration of the oxidant/antioxidant status of the brain, which activates the intrinsic apoptotic pathway. This study revealed that the n-BuOH extract acts by several mechanisms: neutralization of oxidative stress, modulation of markers of apoptosis (Bcl-2, Bax and caspase 3) and neurochemical markers (dopamine and glutamate), and reduction of neuroinflammation which results in decreased MPO activity and lower levels of NO and TNF. The antioxidant and anti-inflammatory properties of this
plant are probably explained by its richness in polyphenols and flavonoids, which have been revealed by phytochemical screening and LC-MS/MS analysis, and/or by the synergistic interactions of the isolated phenolics.
Diplôme : Doctorat En ligne : ../theses/biologie/HAD7693.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11527 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAD/7693 HAD/7693 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation de la composition en microconstituants des margines issues de la production oléicole et utilisabilité comme complément dans la ration chez la vache laitière Type de document : texte imprimé Auteurs : Moufida Aggoun-Arhab, Auteur ; Malika Barkat, Directeur de thèse ; Benoit Graulet, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 178 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Composés phénoliques caroténoïdes tocophérols margines variétés d’olive procédés d’extraction composition chimique vache laitière fermentation ruminale Phenolic compounds carotenoids tocopherols olive wastewater olive variety olive milling chemical composition dairy cow ruminal fermentation المركبات الفينولية الكاروتينات التوكوفيرول المرج أصناف الزيتون طرائق
استخلاص الزيتون التركيب الكيميائي بقرة حلوب ، التخمر في الكرشIndex. décimale : 640 Industrie Alimentaire Résumé : Olive oil production yields a considerable amount of wastewater, a powerful pollutant
that is currently discarded but could be considered as a potential source of valuable natural products due to its content in phenolic compounds and other natural antioxidants. The aim of this work was to valorize this by-product by ruminants. Thus, the proposed strategy was to distribute olive mill wastewater (OMWW) to dairy cows in production. This large-scale recovery required two prior steps: i) explore the variability in OMWW composition from Algerian olive oil mills considering extraction processes (traditional discontinuous press vs 3-phases centrifugal system)
and olive varieties (Azerradj, Sigoise, Chemlal) and, ii) estimate its in vitro degradability in presence of ruminale microbiota in batch systems. Whereas pH, dry or organic matter content didn't vary, there was a significant difference in ash content according to extraction process and olive variety. Carotenoid content was 2.2-fold higher with 3-phases than with press systems whereas tocopherol content was not significantly different. Among the phenolic compounds quantified, tyrosol was usually the most abundant whereas oleuropein concentrations were highly variable. Differences in phenolic compound concentrations were more pronounced between olive varieties than between processes.
The anaerobic biodegradation of OMWW reveals their extensive use by the rumen microbiota. Compared to vetch hay oats, they produce a small amount of gas. In addition, in vitro fermentation generates a low volume of methane. This allows OMWW to improve the efficiency of ruminal microbial flora in the biomass production and inhibition of methane production, a major source of energy loss for the animal. This result allows us to recommend their use as a food additive in the diet of ruminants
The distribution of OMWW in the diet of dairy cows has allowed us to define the
conditions for presentation of OMWW to dairy cows (shape, adaptation, and intake level) and their acceptability. Our results showed that the level of quantitative output (milk production) and fine composition of milk (vitamin A, E and phenolic compounds) did not seem to be affected significantly. The lack of significant differences between groups of animals is due to the fact that the OMWW distributed to the cows were not particularly rich in micro-components compared to forage.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/AGG6913.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10265 Caractérisation de la composition en microconstituants des margines issues de la production oléicole et utilisabilité comme complément dans la ration chez la vache laitière [texte imprimé] / Moufida Aggoun-Arhab, Auteur ; Malika Barkat, Directeur de thèse ; Benoit Graulet, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 178 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Composés phénoliques caroténoïdes tocophérols margines variétés d’olive procédés d’extraction composition chimique vache laitière fermentation ruminale Phenolic compounds carotenoids tocopherols olive wastewater olive variety olive milling chemical composition dairy cow ruminal fermentation المركبات الفينولية الكاروتينات التوكوفيرول المرج أصناف الزيتون طرائق
استخلاص الزيتون التركيب الكيميائي بقرة حلوب ، التخمر في الكرشIndex. décimale : 640 Industrie Alimentaire Résumé : Olive oil production yields a considerable amount of wastewater, a powerful pollutant
that is currently discarded but could be considered as a potential source of valuable natural products due to its content in phenolic compounds and other natural antioxidants. The aim of this work was to valorize this by-product by ruminants. Thus, the proposed strategy was to distribute olive mill wastewater (OMWW) to dairy cows in production. This large-scale recovery required two prior steps: i) explore the variability in OMWW composition from Algerian olive oil mills considering extraction processes (traditional discontinuous press vs 3-phases centrifugal system)
and olive varieties (Azerradj, Sigoise, Chemlal) and, ii) estimate its in vitro degradability in presence of ruminale microbiota in batch systems. Whereas pH, dry or organic matter content didn't vary, there was a significant difference in ash content according to extraction process and olive variety. Carotenoid content was 2.2-fold higher with 3-phases than with press systems whereas tocopherol content was not significantly different. Among the phenolic compounds quantified, tyrosol was usually the most abundant whereas oleuropein concentrations were highly variable. Differences in phenolic compound concentrations were more pronounced between olive varieties than between processes.
The anaerobic biodegradation of OMWW reveals their extensive use by the rumen microbiota. Compared to vetch hay oats, they produce a small amount of gas. In addition, in vitro fermentation generates a low volume of methane. This allows OMWW to improve the efficiency of ruminal microbial flora in the biomass production and inhibition of methane production, a major source of energy loss for the animal. This result allows us to recommend their use as a food additive in the diet of ruminants
The distribution of OMWW in the diet of dairy cows has allowed us to define the
conditions for presentation of OMWW to dairy cows (shape, adaptation, and intake level) and their acceptability. Our results showed that the level of quantitative output (milk production) and fine composition of milk (vitamin A, E and phenolic compounds) did not seem to be affected significantly. The lack of significant differences between groups of animals is due to the fact that the OMWW distributed to the cows were not particularly rich in micro-components compared to forage.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/AGG6913.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10265 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AGG/6913 AGG/6913 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et biologique de deux plantes médicinales Algériennes : Thymus ciliatus (Desf) (Lamiaceae) et Asteriscus graveolens (Forsk.) Dc. (Asteraceae). Type de document : texte imprimé Auteurs : Hanane Achoub, Auteur ; Lahcene Zaiter, Directeur de thèse Mention d'édition : 30/11/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 267 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie organique: Phytochimie Thymus ciliatus (Desf) Asteriscus graveolens (Forsk.) dc. Lamiaceae Asteraceae sesquiterpènes flavonoïdes composés phénoliques monoterpènes activité antioxydante huile essentielle cytotoxicité sesquiterpenes flavonoids phenolic compounds monoterpenes Antioxidant activity essential oil cytotoxic effect سسكويثربينات فلافونويد مركبات فينولية أحادية التربينات نشاط مضادات الأكسدة الزيت الأساسي تأثير السمي للخلايا Index. décimale : 540 Chimie et sciences connexes Résumé :
This work concerned the phytochemical and biological study of two Algerian medicinal plants Thymus ciliatus (Desf) and Asteriscus graveolens (Forsk.) dc. belonging to the families Lamiaceae and Asteraceae ,respectively. This study led to the isolation of twenty seven products from which seventeen were identified including. Among the isolated compounds, 5 sesquiterpenes from which 3 were new, 6 flavonoids which 1 is new, 4 phenolic compounds and 2 monoterpenes. The structures of the isolated products were elucidated mainly by the combination of spectroscopic methods mainly: NMR techniques (1H, 13C, COSY, HSQC and HMBC), UV spectrophotometry, we also used high resolution mass spectrometry methods and comparison with literature data. The quantification of total phenols and total flavonoids contents of the EtOAc, nBuOH extracts of the aerial parts of Thymus ciliatus (Desf) were determined, EtOAc extract had the highest phenolic and flavonoid contents followed by n-butanol extract. This extracts showed an excellent antioxidant activity by DPPH test. The antioxidant activity of AcOEt and n-BuOH extracts of Asteriscus graveolens was estimated by the DPPH test and β-Carotene bleaching assay. The best result was obtained for the ethyl acetate extract, especially in the DPPH method, where its effect is greater than BHT and α-tocopherol used as standards. Both extracts and some isolated compounds of Asteriscus graveolens were tested for their cytotoxic effect in human cancer cell lines (HCT-116, DLD1 and HDFa). The results indicate that EtOAc extract and isolated compounds 9 α-hydroxyparthenolide, 9β-hydroxyparthenolide et 7,12-dihydroxy-6,7- dihydro-5,(6) E-dehydronerolidol showed significant activity compared to the n-BuOH extract and and the two new compounds. The chemical composition of the essential oil of aerial parts of Thymus ciliatus (Desf) Benth studied by GC-FID and GC-MS showed that this oil is mainly composed of elemol (6.80%), carvacrol (5.86%), γ-muurolene (5.18%), β-sesquiphellandrene (5.09%), bicyclogermacrene (5.04%), β-Pinene (4.49%) and curcumene (4.20%).Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/chimie/ACH7683.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11517 Etude phytochimique et biologique de deux plantes médicinales Algériennes : Thymus ciliatus (Desf) (Lamiaceae) et Asteriscus graveolens (Forsk.) Dc. (Asteraceae). [texte imprimé] / Hanane Achoub, Auteur ; Lahcene Zaiter, Directeur de thèse . - 30/11/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 267 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie organique: Phytochimie Thymus ciliatus (Desf) Asteriscus graveolens (Forsk.) dc. Lamiaceae Asteraceae sesquiterpènes flavonoïdes composés phénoliques monoterpènes activité antioxydante huile essentielle cytotoxicité sesquiterpenes flavonoids phenolic compounds monoterpenes Antioxidant activity essential oil cytotoxic effect سسكويثربينات فلافونويد مركبات فينولية أحادية التربينات نشاط مضادات الأكسدة الزيت الأساسي تأثير السمي للخلايا Index. décimale : 540 Chimie et sciences connexes Résumé :
This work concerned the phytochemical and biological study of two Algerian medicinal plants Thymus ciliatus (Desf) and Asteriscus graveolens (Forsk.) dc. belonging to the families Lamiaceae and Asteraceae ,respectively. This study led to the isolation of twenty seven products from which seventeen were identified including. Among the isolated compounds, 5 sesquiterpenes from which 3 were new, 6 flavonoids which 1 is new, 4 phenolic compounds and 2 monoterpenes. The structures of the isolated products were elucidated mainly by the combination of spectroscopic methods mainly: NMR techniques (1H, 13C, COSY, HSQC and HMBC), UV spectrophotometry, we also used high resolution mass spectrometry methods and comparison with literature data. The quantification of total phenols and total flavonoids contents of the EtOAc, nBuOH extracts of the aerial parts of Thymus ciliatus (Desf) were determined, EtOAc extract had the highest phenolic and flavonoid contents followed by n-butanol extract. This extracts showed an excellent antioxidant activity by DPPH test. The antioxidant activity of AcOEt and n-BuOH extracts of Asteriscus graveolens was estimated by the DPPH test and β-Carotene bleaching assay. The best result was obtained for the ethyl acetate extract, especially in the DPPH method, where its effect is greater than BHT and α-tocopherol used as standards. Both extracts and some isolated compounds of Asteriscus graveolens were tested for their cytotoxic effect in human cancer cell lines (HCT-116, DLD1 and HDFa). The results indicate that EtOAc extract and isolated compounds 9 α-hydroxyparthenolide, 9β-hydroxyparthenolide et 7,12-dihydroxy-6,7- dihydro-5,(6) E-dehydronerolidol showed significant activity compared to the n-BuOH extract and and the two new compounds. The chemical composition of the essential oil of aerial parts of Thymus ciliatus (Desf) Benth studied by GC-FID and GC-MS showed that this oil is mainly composed of elemol (6.80%), carvacrol (5.86%), γ-muurolene (5.18%), β-sesquiphellandrene (5.09%), bicyclogermacrene (5.04%), β-Pinene (4.49%) and curcumene (4.20%).Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/chimie/ACH7683.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11517 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ACHO/7683 ACHO/7683 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et évaluation des activités biologiques de la plante Daucus muricatus (Apiaceae) Type de document : texte imprimé Auteurs : Amira Noui, Auteur ; Salah Akkal, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 85 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Apiaceae Daucus muricatus LC-ESI-MS/MS Composés phénoliques Activité antioxydante phenolic compounds antioxidant activity العائلة الخيمية البوليفينولات الفعالية المضادة للاكسدة Index. décimale : 540 Chimie et sciences connexes Résumé : This work is dedicate to the phytochemical study of Daucus muricatus species (Apiaceae), it was aimed at investigating the phytochemical components of butanolic extract using HPLC-DAD-ESI-MS/MS. The proposed method provided tentative identification of 25 phenolic compounds, including, 13 hydroxycinnamic acid derivatives, 5 hydroxybenzoïc acid derivatives,8 flavonoïds and other polar
compounds as quinic acid. Evaluation of the antioxidant activities by using four complementary tests (DPPH, ABTS, CUPRAC and carotene Bleanching) showed that both extracts exhibited importatnt antioxidant activities.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/NOU7349.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10999 Etude phytochimique et évaluation des activités biologiques de la plante Daucus muricatus (Apiaceae) [texte imprimé] / Amira Noui, Auteur ; Salah Akkal, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 85 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Apiaceae Daucus muricatus LC-ESI-MS/MS Composés phénoliques Activité antioxydante phenolic compounds antioxidant activity العائلة الخيمية البوليفينولات الفعالية المضادة للاكسدة Index. décimale : 540 Chimie et sciences connexes Résumé : This work is dedicate to the phytochemical study of Daucus muricatus species (Apiaceae), it was aimed at investigating the phytochemical components of butanolic extract using HPLC-DAD-ESI-MS/MS. The proposed method provided tentative identification of 25 phenolic compounds, including, 13 hydroxycinnamic acid derivatives, 5 hydroxybenzoïc acid derivatives,8 flavonoïds and other polar
compounds as quinic acid. Evaluation of the antioxidant activities by using four complementary tests (DPPH, ABTS, CUPRAC and carotene Bleanching) showed that both extracts exhibited importatnt antioxidant activities.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/NOU7349.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10999 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité NOU/7349 NOU/7349 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et pharmacologique de Crataegus oxyacantha L. (Rosaceae) et Cytisus triflorus L’Her. (Fabaceae). Type de document : texte imprimé Auteurs : Wassila Benabderrahmane, Auteur ; Ouahiba Benaissa, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 234 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie organique: phytochimie Rosaceae Fabaceae Crataegus oxyacantha Cytisus triflorus Flavonoïdes composés phénoliques Activité antioxydante Activité antimicrobienne RMN 1D et 2D HPLC-DAD Flavonoids Phenolic compounds Antioxidant activity Antimicrobial activity 1D and 2D NMR HPLCDAD فلافونويدات مركبات فينولية النشاط المضاد للأكسدة النشاط المصاد للميكروبات Index. décimale : 540 Chimie et sciences connexes Résumé :
The work presented in this thesis contributes to the valorization of both Algerian medicinal plants (Crataegus oxyacantha L. (Rosaceae) and Cytisus triflorus L'Her. (Fabaceae) by characterizing them by a phytochemical study, an evaluation of the antioxidant and antimicrobial activities, and a structural identification of certain phenolic compounds. Thus, in order to obtain an extract enriched in metabolites of interest, flavonoids, we opted for two extraction methods, a liquid solid extraction using different solvents of increasing polarity (CHCl3, EtOAc and n-Butanol) and a second extraction using the Matrix solid phase dispersion (MSPD) method, using different solvents of increasing polarity (100% H2O, 1:1 H2O / EtOH and 100% EtOH).
This study allowed the isolation 21 compounds and structural elucidation of 16 compounds.
Of these 16 compounds, 3 acids and 7 flavonoids: (3 flavonols including 1 glucosyl, 1 glucosyl flavanone, 1 Flavanol and 2 flavones) were isolated from C. oxyacantha L. From the EtOAc extract the aerial parts (Flowers + Leaves) of the species Cytisus triflorus L'Her., 6 secondary metabolites were identified (flavonoids). Among these compounds, 3 flavones: (Chrysin, Prunetin) and 3 glucosyl flavonoids (2 Chrysine and Isoquercitrin). Of these isolated compounds, 3 new in the species. These major active ingredients possess various biological activities and play an important and acknowledged role in maintaining good health. The structures of the isolated compounds were established by HPLC-DAD chromatogram analysis, UV-vis spectra and high resolution electrospray mass spectra (HRESI-MS), by extensive use and interpretation of NMR experiments spectra : 1D NMR (1H, 13C and DEPT) and 2D NMR (COSY, HSQC and HMBC) and by comparison with the literature data. The in vitro evaluation antioxidant activity of the various extracts and of three isolated products of C. oxyacantha, and the AcOEt extract and three products isolated from C. triflorus was performed by the radical scavenging method (DPPH *). The extracts of two plants have interesting antioxidant activities, dependent on the content of total polyphenols and flavonoids. The various extracts studied were also revealed a high antibacterial activity towards S. aureus. Similarly, a tendency shown indicating that the richest extracts of flavonoids and polyphenols exhibit the higher antibacterial activities.Note de contenu :
Annexe.Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BEN7472.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11304 Etude phytochimique et pharmacologique de Crataegus oxyacantha L. (Rosaceae) et Cytisus triflorus L’Her. (Fabaceae). [texte imprimé] / Wassila Benabderrahmane, Auteur ; Ouahiba Benaissa, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 234 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie organique: phytochimie Rosaceae Fabaceae Crataegus oxyacantha Cytisus triflorus Flavonoïdes composés phénoliques Activité antioxydante Activité antimicrobienne RMN 1D et 2D HPLC-DAD Flavonoids Phenolic compounds Antioxidant activity Antimicrobial activity 1D and 2D NMR HPLCDAD فلافونويدات مركبات فينولية النشاط المضاد للأكسدة النشاط المصاد للميكروبات Index. décimale : 540 Chimie et sciences connexes Résumé :
The work presented in this thesis contributes to the valorization of both Algerian medicinal plants (Crataegus oxyacantha L. (Rosaceae) and Cytisus triflorus L'Her. (Fabaceae) by characterizing them by a phytochemical study, an evaluation of the antioxidant and antimicrobial activities, and a structural identification of certain phenolic compounds. Thus, in order to obtain an extract enriched in metabolites of interest, flavonoids, we opted for two extraction methods, a liquid solid extraction using different solvents of increasing polarity (CHCl3, EtOAc and n-Butanol) and a second extraction using the Matrix solid phase dispersion (MSPD) method, using different solvents of increasing polarity (100% H2O, 1:1 H2O / EtOH and 100% EtOH).
This study allowed the isolation 21 compounds and structural elucidation of 16 compounds.
Of these 16 compounds, 3 acids and 7 flavonoids: (3 flavonols including 1 glucosyl, 1 glucosyl flavanone, 1 Flavanol and 2 flavones) were isolated from C. oxyacantha L. From the EtOAc extract the aerial parts (Flowers + Leaves) of the species Cytisus triflorus L'Her., 6 secondary metabolites were identified (flavonoids). Among these compounds, 3 flavones: (Chrysin, Prunetin) and 3 glucosyl flavonoids (2 Chrysine and Isoquercitrin). Of these isolated compounds, 3 new in the species. These major active ingredients possess various biological activities and play an important and acknowledged role in maintaining good health. The structures of the isolated compounds were established by HPLC-DAD chromatogram analysis, UV-vis spectra and high resolution electrospray mass spectra (HRESI-MS), by extensive use and interpretation of NMR experiments spectra : 1D NMR (1H, 13C and DEPT) and 2D NMR (COSY, HSQC and HMBC) and by comparison with the literature data. The in vitro evaluation antioxidant activity of the various extracts and of three isolated products of C. oxyacantha, and the AcOEt extract and three products isolated from C. triflorus was performed by the radical scavenging method (DPPH *). The extracts of two plants have interesting antioxidant activities, dependent on the content of total polyphenols and flavonoids. The various extracts studied were also revealed a high antibacterial activity towards S. aureus. Similarly, a tendency shown indicating that the richest extracts of flavonoids and polyphenols exhibit the higher antibacterial activities.Note de contenu :
Annexe.Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BEN7472.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11304 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/7472 BEN/7472 Thèse Bibliothèque principale Thèses Disponible
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