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Partager le résultat de cette recherche Interroger des sources externesConception in silico de nouveaux inhibiteurs de la topoisomérase I de cellules cancéreuses. / Soumia Teniou
Titre : Conception in silico de nouveaux inhibiteurs de la topoisomérase I de cellules cancéreuses. Type de document : texte imprimé Auteurs : Soumia Teniou, Auteur ; Abdellatif Bensegueni, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 95 f. Format : 31 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie:Technologie des Explorations Biochimiques L'ADN Topoisomérase I Flavonoides Modélisation moléculaire Docking moléculaire Surflex Affinité Myricétine DNA topoisomérase I Flavonoids Molecular modeling Molecular Docking Affinity Myricetin الفلافونويدات النمذجة الجزيئية الجاذبية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Human DNA Topoisomérase I (topo I) is the target of several drugs commonly used in cancer chemotherapy. These drugs induce topo-DNA complexes that eventually trigger cell death. Several flavonoides have been shown as inhibitors of topo I, having an anticancer activity. Using the methods of molecular modeling in particularl the docking by the Surflex program, we theoretically evaluated the affinity of four flavonoids: myricetin, fisetin, quercetin and apigenin, presented in recent studies as inhibitors of topo I. Myricetin with the lowest IC50 (11.1 ± 2.0 µM) gave the best affinity (7.01 M-1). For the development in silico of novel molecules most powerful myricetin, we have made several substitutions. Replacing the hydroxyl group carried by the carbone C3´ of the myricetin by an alcoholic group CH2OH significantly improves the affinity increases from 7.01 M-1 to 8.79 M-1. The application of the rule of Lipinski informs in a positive way about the ADME properties of this new molecule that is as an inhibitor potentially more active than myricetin.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/biologie/TEN6172.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=6108 Conception in silico de nouveaux inhibiteurs de la topoisomérase I de cellules cancéreuses. [texte imprimé] / Soumia Teniou, Auteur ; Abdellatif Bensegueni, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 95 f. ; 31 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie:Technologie des Explorations Biochimiques L'ADN Topoisomérase I Flavonoides Modélisation moléculaire Docking moléculaire Surflex Affinité Myricétine DNA topoisomérase I Flavonoids Molecular modeling Molecular Docking Affinity Myricetin الفلافونويدات النمذجة الجزيئية الجاذبية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Human DNA Topoisomérase I (topo I) is the target of several drugs commonly used in cancer chemotherapy. These drugs induce topo-DNA complexes that eventually trigger cell death. Several flavonoides have been shown as inhibitors of topo I, having an anticancer activity. Using the methods of molecular modeling in particularl the docking by the Surflex program, we theoretically evaluated the affinity of four flavonoids: myricetin, fisetin, quercetin and apigenin, presented in recent studies as inhibitors of topo I. Myricetin with the lowest IC50 (11.1 ± 2.0 µM) gave the best affinity (7.01 M-1). For the development in silico of novel molecules most powerful myricetin, we have made several substitutions. Replacing the hydroxyl group carried by the carbone C3´ of the myricetin by an alcoholic group CH2OH significantly improves the affinity increases from 7.01 M-1 to 8.79 M-1. The application of the rule of Lipinski informs in a positive way about the ADME properties of this new molecule that is as an inhibitor potentially more active than myricetin.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/biologie/TEN6172.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=6108 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité TEN/6172 TEN/6172 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation phytochimique et évaluation des activités biologiques de Cleome arabica. Type de document : texte imprimé Auteurs : Aicha Madi, Auteur ; Abdelmalik Belkhiri, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 146 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles. Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Cleome arabica ssp arabica HPLC/DAD/MS phénols totaux flavonoïdes bioactivité toxicité total phenols flavonoids bioactivity toxicity assays الفينولات الفلافونيدات النشاط البيولوجي تجارب السمية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Cleome arabica ssp arabica, a threatened desert plant, has been subjected to the phytochemical and pharmacological screening.
Hydroalcoholic extracts from different parts (leaves, seeds and roots) of C. arabica have been tested for their antioxidant, analgesic and wound healing activities, as well as their toxic potential.
Quantitative evaluation of the total phenols, flavonoids and anthocyanidins contents of the different extracts were made and completed with chromatographic HPLC/DAD/SM analysis. The obtained results revealed that leaves extract was characterized by a highly content in phenolic compounds, compared to other extracts.
Hydroalcoholic extract of the three parts of plant were marked by the presence of flavonoids, specially of flavonols type (quercetin, keampferol, and isorhamnetin).
Phenolic acids content is marked by the presence in the seeds extract of ferulic acid, para-hydroxybenzoic acid, and para-coumaric acid, and in the roots extract of tricaffeoylquinic, gallic, ferulic, and vanillic acids.
Evaluation of antioxidant effect made by the three in vitro tests (DPPH, FRAP and Phosphomolybdenum) showed a highly antioxidant activity of the leaves extract compared to those of seeds and roots. A direct correlation was found between phenol total and flavonoids contents and antioxidant activity.
The effectives doses obtained by three pain tests, namely tail flick, hot plate and acetic acid test were 291,84mg/Kg, 848,53mg/Kg and 683,52mg/Kg respectively. Analgesic activity of the aerial part of C. arabica was found to be dose- dependent. The Hydroalcoholic extract displayed on excision and thermal healing activities
wounds. The wound healing effect of C.arabica was significantly more important than that of a commercial ointment used as a positive control (Cicatryl®).
Toxicity assays (acute toxicity, and hemolytic effect) have showed that Hydroalcoholic extract of aerial part of C. arabica is safe at the maximal described doses.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAD7196.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10767 Caractérisation phytochimique et évaluation des activités biologiques de Cleome arabica. [texte imprimé] / Aicha Madi, Auteur ; Abdelmalik Belkhiri, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 146 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Cleome arabica ssp arabica HPLC/DAD/MS phénols totaux flavonoïdes bioactivité toxicité total phenols flavonoids bioactivity toxicity assays الفينولات الفلافونيدات النشاط البيولوجي تجارب السمية Index. décimale : 570 Sciences de la vie. Biologie Résumé : Cleome arabica ssp arabica, a threatened desert plant, has been subjected to the phytochemical and pharmacological screening.
Hydroalcoholic extracts from different parts (leaves, seeds and roots) of C. arabica have been tested for their antioxidant, analgesic and wound healing activities, as well as their toxic potential.
Quantitative evaluation of the total phenols, flavonoids and anthocyanidins contents of the different extracts were made and completed with chromatographic HPLC/DAD/SM analysis. The obtained results revealed that leaves extract was characterized by a highly content in phenolic compounds, compared to other extracts.
Hydroalcoholic extract of the three parts of plant were marked by the presence of flavonoids, specially of flavonols type (quercetin, keampferol, and isorhamnetin).
Phenolic acids content is marked by the presence in the seeds extract of ferulic acid, para-hydroxybenzoic acid, and para-coumaric acid, and in the roots extract of tricaffeoylquinic, gallic, ferulic, and vanillic acids.
Evaluation of antioxidant effect made by the three in vitro tests (DPPH, FRAP and Phosphomolybdenum) showed a highly antioxidant activity of the leaves extract compared to those of seeds and roots. A direct correlation was found between phenol total and flavonoids contents and antioxidant activity.
The effectives doses obtained by three pain tests, namely tail flick, hot plate and acetic acid test were 291,84mg/Kg, 848,53mg/Kg and 683,52mg/Kg respectively. Analgesic activity of the aerial part of C. arabica was found to be dose- dependent. The Hydroalcoholic extract displayed on excision and thermal healing activities
wounds. The wound healing effect of C.arabica was significantly more important than that of a commercial ointment used as a positive control (Cicatryl®).
Toxicity assays (acute toxicity, and hemolytic effect) have showed that Hydroalcoholic extract of aerial part of C. arabica is safe at the maximal described doses.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAD7196.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10767 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MAD/7196 MAD/7196 Thèse Bibliothèque principale Thèses Disponible
Titre : Effet des extraits de la plante médicinale Ruta montana) (الفيجلsur la cardiotoxicité induite par la doxorubicine et sur la multi-drug résistances (MDR) des cellules cancéreuses ovarien (A2780) Type de document : texte imprimé Auteurs : Wahiba Kara Ali, Auteur ; N. Abidli, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 160 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Ruta montana Doxorubicine Cardiotoxicité Multi-drug résistance Stress oxydant Antioxydant Flavonoides Cardiotoxicity Multidrug resistance Oxidative
stress Antioxidant Flavonoids الدوكسوروبسين السمية القلبية المقاومة المتعددة للأدوية الإجهاد التاكسدي النشاط المضاد للأكسدة الفلافونويداتIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for the preventive effect of medicinal plant Ruta montana
extract on oxidative stress induced by doxorubicine (DOX) and its properties to reverse
doxorubicin resistance in ovarian cancer cell line (A2780). Organic extracts were obtained by
maceration with three solvents: petroleum ether, ethyl acetate and butanol. The yields were:
15,04%, 3,55% and 25,14%.respectively. A phytochemical screening in methanol extract
revealed that the plant contain the flavonoids, saponins, tannins, coumarins, reducing component
and the alkaloids. The quantitative study showed that the butanolic extract (BERM) has
significant amount of flavonoids (80.85±0.78mg EQ / g dry extract), followed by ethyl acetate
extract (AEERM) (15.27 ±0.62 EQ / g dry extract) and petroleum ether extract has the lowest
flavonoids content (PEERM) (9.71±1.13 mgEQ/g dry extract). When the total phenolic contents
was: 687.5, 595.83 and 146.33 mg EGA / g dry extract, in the BERM, AEERM and PEERM
respectively. The qualitative analysis of the three methanolic crude extract via thin layer
chromatography (TLC) has shown the presence of the anthocyanidin 3-glycosids, flavons et
flavonols glycosids (hétérosids), flavonols avec 3-OH libre, flavonons ou aurons, isoflavons, the
phénolic acids, Flavonols et 4-OH chalcons. The évaluation of antioxidant activity by the
reduction of free radical DPPH method showed that The highest DPPH radical scavenging
activity was observed with AEERM (IC50 = 0.126 ± 0.002 mg/ ml), followed by BERM (0.135
± 0.0006 mg/ ml) and PEERM (IC50 =0.185± 0.002 mg/ ml). The study of preventive effect of
AEERM on oxidative stress induced by DOX in wistar rats clearly show that DOX (15 mg/kg
i.p) induced animal cardiotoxicity characterized by an increase of cardiac enzyme activity
(CKMB, LDH, l’ALAT et l’ASAT) in serum, the increase in the concentration of
malondialdéhyde (MDA), antioxydant enzyme activity, catalase (CAT) and Glutathion- Stransférase (GST) and a decrease of cardiac cytosolic reduced glutathione (GSH) with a different
pathological changes in the heart. However pretreated animals with EAERM (100 mg/kg orally
during 15 days), showed a decrease of cardiac enzymes activities 24 %, 35, 69%, 21,25 % et
8,10 % respectively to CKMB, LDH, l’ALAT and l’ASAT. The preatretment with the extract
improved antioxidant status in the heart by a decrease in the cardiac MDA concentration by 29,7
% and activity of antioxydants enzymes by 17,91% et 29,78% respectively to CAT et la GST.
In addition, the pretreated rats by the extract showed an increase of GSH level (83,76 %)
compared with DOX-treated animals. Histological study shows that the extract resulted in a
reduction of severe cardiac damages observed in DOX-treated animals. The reversal of
multidrug resistance for DOX in human ovarian adenocarcinoma, cell lines (A2780 DX3) using
MTT assay showed that AEERM was very effective at reversing the resistant in dose dependent
manner with the Reversion Factor (RF =8.62, 05.77 and 02.30 respectively for the
concentrations 40, 10 and 2.5 mg/ml) followed by BERM (FR=05.07 and 02.43 for the
concentrations 40 and 10 mg/ml respectively), then the PEERM had the lowest reversal
resistance effect (RF betwen 1.01 to 1.08). The present study suggests that AEERM can
ameliorate the oxidative stress involved in the pathogenesis of doxorubicine- induced
cardiotoxicity and can reverse the multidrug resistance for DOX.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAR7158.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10435 Effet des extraits de la plante médicinale Ruta montana) (الفيجلsur la cardiotoxicité induite par la doxorubicine et sur la multi-drug résistances (MDR) des cellules cancéreuses ovarien (A2780) [texte imprimé] / Wahiba Kara Ali, Auteur ; N. Abidli, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 160 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Ruta montana Doxorubicine Cardiotoxicité Multi-drug résistance Stress oxydant Antioxydant Flavonoides Cardiotoxicity Multidrug resistance Oxidative
stress Antioxidant Flavonoids الدوكسوروبسين السمية القلبية المقاومة المتعددة للأدوية الإجهاد التاكسدي النشاط المضاد للأكسدة الفلافونويداتIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for the preventive effect of medicinal plant Ruta montana
extract on oxidative stress induced by doxorubicine (DOX) and its properties to reverse
doxorubicin resistance in ovarian cancer cell line (A2780). Organic extracts were obtained by
maceration with three solvents: petroleum ether, ethyl acetate and butanol. The yields were:
15,04%, 3,55% and 25,14%.respectively. A phytochemical screening in methanol extract
revealed that the plant contain the flavonoids, saponins, tannins, coumarins, reducing component
and the alkaloids. The quantitative study showed that the butanolic extract (BERM) has
significant amount of flavonoids (80.85±0.78mg EQ / g dry extract), followed by ethyl acetate
extract (AEERM) (15.27 ±0.62 EQ / g dry extract) and petroleum ether extract has the lowest
flavonoids content (PEERM) (9.71±1.13 mgEQ/g dry extract). When the total phenolic contents
was: 687.5, 595.83 and 146.33 mg EGA / g dry extract, in the BERM, AEERM and PEERM
respectively. The qualitative analysis of the three methanolic crude extract via thin layer
chromatography (TLC) has shown the presence of the anthocyanidin 3-glycosids, flavons et
flavonols glycosids (hétérosids), flavonols avec 3-OH libre, flavonons ou aurons, isoflavons, the
phénolic acids, Flavonols et 4-OH chalcons. The évaluation of antioxidant activity by the
reduction of free radical DPPH method showed that The highest DPPH radical scavenging
activity was observed with AEERM (IC50 = 0.126 ± 0.002 mg/ ml), followed by BERM (0.135
± 0.0006 mg/ ml) and PEERM (IC50 =0.185± 0.002 mg/ ml). The study of preventive effect of
AEERM on oxidative stress induced by DOX in wistar rats clearly show that DOX (15 mg/kg
i.p) induced animal cardiotoxicity characterized by an increase of cardiac enzyme activity
(CKMB, LDH, l’ALAT et l’ASAT) in serum, the increase in the concentration of
malondialdéhyde (MDA), antioxydant enzyme activity, catalase (CAT) and Glutathion- Stransférase (GST) and a decrease of cardiac cytosolic reduced glutathione (GSH) with a different
pathological changes in the heart. However pretreated animals with EAERM (100 mg/kg orally
during 15 days), showed a decrease of cardiac enzymes activities 24 %, 35, 69%, 21,25 % et
8,10 % respectively to CKMB, LDH, l’ALAT and l’ASAT. The preatretment with the extract
improved antioxidant status in the heart by a decrease in the cardiac MDA concentration by 29,7
% and activity of antioxydants enzymes by 17,91% et 29,78% respectively to CAT et la GST.
In addition, the pretreated rats by the extract showed an increase of GSH level (83,76 %)
compared with DOX-treated animals. Histological study shows that the extract resulted in a
reduction of severe cardiac damages observed in DOX-treated animals. The reversal of
multidrug resistance for DOX in human ovarian adenocarcinoma, cell lines (A2780 DX3) using
MTT assay showed that AEERM was very effective at reversing the resistant in dose dependent
manner with the Reversion Factor (RF =8.62, 05.77 and 02.30 respectively for the
concentrations 40, 10 and 2.5 mg/ml) followed by BERM (FR=05.07 and 02.43 for the
concentrations 40 and 10 mg/ml respectively), then the PEERM had the lowest reversal
resistance effect (RF betwen 1.01 to 1.08). The present study suggests that AEERM can
ameliorate the oxidative stress involved in the pathogenesis of doxorubicine- induced
cardiotoxicity and can reverse the multidrug resistance for DOX.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAR7158.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10435 Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Titre : Essais d'optimisation des conditions de cuisson et d'extraction des polyphénols de six légumes et évaluation des activités : antioxydante et anticholinestérase. Type de document : texte imprimé Auteurs : Imène Laib, Auteur ; Malika Barkat, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 287 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Optimisation légume cuisson polyphénols totaux flavonoïdes activité antioxydante activité anticholinestérase Optimization vegetable cooking total polyphenolic flavonoids antioxidant activity anticholinesterase activity لتحسين الخضار الطبخ البوليفينول الكلي الفلافونويد النشاط المضاد للأكسدة نشاط مضادات
الكولينستريزIndex. décimale : 640 Industrie Alimentaire Résumé : This study focuses on the optimization of the cooking conditions and the extraction of polyphenols from six vegetables (potato, onion, spinach, aubergine carrot and pepper) and the study of the impact of the cooking method (boiling, steaming and microwaving) on these compounds and their biological activities (antioxidant activity and anticholinesterase activity).
To evaluate the quantity of phenolic compounds extracted from raw and cooked vegetables, a experiment design was realized. The factors chosen are (Temperature, time, power) for cooking and (solvent concentration, maceration time, ultrasonication and grinding) for polyphenol extraction . The study was initiated by Plackett-Burmann design to perform the screening of factors and to select the most influential on the Y response (total phenolic) and an optimization by exploiting central composite design. Determination of total polyphenols, flavonoids and LC-MS / MS analysis were performed to monitor the variation of phenolic compounds after cooking. In addition, in this work, we anlyzed antioxidant and anticholinesterase properties of the phenolic compounds of the six raw and cooked vegetables.
The results obtained for the six vegetables are different, boiling in water and steam cooking methods have reduced the levels of total polyphenols and flavonoids of the six vegetables. While microwave cooking had different effects on the six vegetables, it had a negative effect on the total polyphenol and flavonoid levels of the potato, spinach, carrot and pepper.
However, a positive effect was recorded on the total polyphenols and flavonoids of eggplant. Retention of these compounds has been observed in onions cooked by microwave. The impact of cooking on antioxidant activity varies from one vegetable to another. Cooking caused a decrease in the antioxidant activity of potatoes, onions, carrots, peppers and steamed spinach.
A positive effect of cooking was recorded on the antioxidant potential of spinach (cooked in water and by microwave) and eggplant. All three cooking methods showed negative effects on the anticholinesterase activity of potato, spinach and pepper. However, an increase in this activity was recorded for onion, eggplant and carrot. Cooking in water proved to be the worst method for preserving the polyphenols from cooked vegetables and their activities (antioxidant activity and anticholinesterase). The losses of polyphenols are due to their increased solubility in water. However, microwave cooking is suggested as the best method to preserve the polyphenol levels, the antioxidant and anticholinesterase activities of the studied vegetables.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/LAI7279.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10894 Essais d'optimisation des conditions de cuisson et d'extraction des polyphénols de six légumes et évaluation des activités : antioxydante et anticholinestérase. [texte imprimé] / Imène Laib, Auteur ; Malika Barkat, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 287 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Optimisation légume cuisson polyphénols totaux flavonoïdes activité antioxydante activité anticholinestérase Optimization vegetable cooking total polyphenolic flavonoids antioxidant activity anticholinesterase activity لتحسين الخضار الطبخ البوليفينول الكلي الفلافونويد النشاط المضاد للأكسدة نشاط مضادات
الكولينستريزIndex. décimale : 640 Industrie Alimentaire Résumé : This study focuses on the optimization of the cooking conditions and the extraction of polyphenols from six vegetables (potato, onion, spinach, aubergine carrot and pepper) and the study of the impact of the cooking method (boiling, steaming and microwaving) on these compounds and their biological activities (antioxidant activity and anticholinesterase activity).
To evaluate the quantity of phenolic compounds extracted from raw and cooked vegetables, a experiment design was realized. The factors chosen are (Temperature, time, power) for cooking and (solvent concentration, maceration time, ultrasonication and grinding) for polyphenol extraction . The study was initiated by Plackett-Burmann design to perform the screening of factors and to select the most influential on the Y response (total phenolic) and an optimization by exploiting central composite design. Determination of total polyphenols, flavonoids and LC-MS / MS analysis were performed to monitor the variation of phenolic compounds after cooking. In addition, in this work, we anlyzed antioxidant and anticholinesterase properties of the phenolic compounds of the six raw and cooked vegetables.
The results obtained for the six vegetables are different, boiling in water and steam cooking methods have reduced the levels of total polyphenols and flavonoids of the six vegetables. While microwave cooking had different effects on the six vegetables, it had a negative effect on the total polyphenol and flavonoid levels of the potato, spinach, carrot and pepper.
However, a positive effect was recorded on the total polyphenols and flavonoids of eggplant. Retention of these compounds has been observed in onions cooked by microwave. The impact of cooking on antioxidant activity varies from one vegetable to another. Cooking caused a decrease in the antioxidant activity of potatoes, onions, carrots, peppers and steamed spinach.
A positive effect of cooking was recorded on the antioxidant potential of spinach (cooked in water and by microwave) and eggplant. All three cooking methods showed negative effects on the anticholinesterase activity of potato, spinach and pepper. However, an increase in this activity was recorded for onion, eggplant and carrot. Cooking in water proved to be the worst method for preserving the polyphenols from cooked vegetables and their activities (antioxidant activity and anticholinesterase). The losses of polyphenols are due to their increased solubility in water. However, microwave cooking is suggested as the best method to preserve the polyphenol levels, the antioxidant and anticholinesterase activities of the studied vegetables.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/LAI7279.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10894 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAI/7279 LAI/7279 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude comparative du profil chimique et de l’activité antioxydante de plusieurs propolis de l’Est algérien et investigation phytochimique de la propolis la plus active. Type de document : texte imprimé Auteurs : Amina Bouaroura ép Redjem, Auteur ; Narimane Segueni, Directeur de thèse Mention d'édition : 25/06/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 152 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie pharmaceutique:Analyse physicochimique, contrôle de la qualité et synthèse de substances bioactives Propolis étude comparative activité antioxydante activité anticholinestérase polyphénols flavonoïdes LC-MS comparative study antioxidant activitie anticholinesterase activitie phenolics flavonoids العكبر المضاد للأكسدة المضاد للكولينستراز جهاز الكروماتوغرافي السائلة المقترن بمقياس الطيف الكتلي الفلافونويد عديدات الفينول Index. décimale : 540 Chimie et sciences connexes Résumé :
Our study is interested in the investigation of the chemical composition and the biological activities of Algerian propolis. Due to its several biological activities, propolis has been extensively used in folk medicine. In the first part of our study we examined several propolis harvested from north of Algeria in order to compare their antioxydant activity, In addition, the total phenolic and flavonoid contents were evaluated. Propolis of Constantine exhibited the highest antioxydant activity. This result encouraged us to study the anticholinesterase activity of the extracts of this propolis. This first part of our work allowed us to make a first classification of the propolis studied and to select the most active extracts on which we performed an analysis by liquid chromatography coupled to mass spectrophotometry LC-MS for the purpose of studying their chemical profile. The ethyl acetate extract of propolis of Constantine was the most active extract. This extract has the highest levels of caffeic acid, p-coumaric acid, naringenin and kaempferol. Our results suggest that the antioxydant effect is probably due to the presence of these compounds. In the second part of our study we investigated the chemical composition of Algerian propolis. The separation and purification of propolis extracts lead to the identification of 8 compounds, five flavonoids (tectochryin, pinocembrine, pinobksin-3-acetate, chrysin, galangin), one diterpene (cupressic acid) and two derivatives of caffeic acid (3-methyl-3-butenyl (E) -Caffeate and 2-methyl-2 -butenyl (E) -Caffeate). Their structures has been
established by spectral analysis including NMR mono (NMR-1H, NMR-13C) and bidimensionelle spectra (HSQC, HMBC) and impact electronic mass spectra.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7640.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11474 Etude comparative du profil chimique et de l’activité antioxydante de plusieurs propolis de l’Est algérien et investigation phytochimique de la propolis la plus active. [texte imprimé] / Amina Bouaroura ép Redjem, Auteur ; Narimane Segueni, Directeur de thèse . - 25/06/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 152 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie pharmaceutique:Analyse physicochimique, contrôle de la qualité et synthèse de substances bioactives Propolis étude comparative activité antioxydante activité anticholinestérase polyphénols flavonoïdes LC-MS comparative study antioxidant activitie anticholinesterase activitie phenolics flavonoids العكبر المضاد للأكسدة المضاد للكولينستراز جهاز الكروماتوغرافي السائلة المقترن بمقياس الطيف الكتلي الفلافونويد عديدات الفينول Index. décimale : 540 Chimie et sciences connexes Résumé :
Our study is interested in the investigation of the chemical composition and the biological activities of Algerian propolis. Due to its several biological activities, propolis has been extensively used in folk medicine. In the first part of our study we examined several propolis harvested from north of Algeria in order to compare their antioxydant activity, In addition, the total phenolic and flavonoid contents were evaluated. Propolis of Constantine exhibited the highest antioxydant activity. This result encouraged us to study the anticholinesterase activity of the extracts of this propolis. This first part of our work allowed us to make a first classification of the propolis studied and to select the most active extracts on which we performed an analysis by liquid chromatography coupled to mass spectrophotometry LC-MS for the purpose of studying their chemical profile. The ethyl acetate extract of propolis of Constantine was the most active extract. This extract has the highest levels of caffeic acid, p-coumaric acid, naringenin and kaempferol. Our results suggest that the antioxydant effect is probably due to the presence of these compounds. In the second part of our study we investigated the chemical composition of Algerian propolis. The separation and purification of propolis extracts lead to the identification of 8 compounds, five flavonoids (tectochryin, pinocembrine, pinobksin-3-acetate, chrysin, galangin), one diterpene (cupressic acid) and two derivatives of caffeic acid (3-methyl-3-butenyl (E) -Caffeate and 2-methyl-2 -butenyl (E) -Caffeate). Their structures has been
established by spectral analysis including NMR mono (NMR-1H, NMR-13C) and bidimensionelle spectra (HSQC, HMBC) and impact electronic mass spectra.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7640.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11474 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7640 BOU/7640 Thèse Bibliothèque principale Thèses Disponible
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