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Partager le résultat de cette recherche Interroger des sources externesEffet d’extrait méthanolique de la plante médicinale Phlomis samia ()الخیاطةsur l’apoptose des cellules cancéreuses HepG2 et MDA MB 468 et sur la cardiotoxicité induite par la Doxorubicine. / Safia Ihoual ép Fethi
Titre : Effet d’extrait méthanolique de la plante médicinale Phlomis samia ()الخیاطةsur l’apoptose des cellules cancéreuses HepG2 et MDA MB 468 et sur la cardiotoxicité induite par la Doxorubicine. Type de document : texte imprimé Auteurs : Safia Ihoual ép Fethi, Auteur ; Nacira Abidli, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 179 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : le cancer Apoptose Phlomis samia la doxorubicine les radicaux libres cardiotoxicité l’anti oxydant apoptosis doxorubicin Ros cardiotoxicity antioxidant
السرطان الموت المبرمج للخلايا Phlomis samia(الخياطة) الجدور الحرة مضادات الاكسدة السمية القلبيةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The aim of this work was to investigate the effect of the aerial parts Méthanolic extract of Phlomis samia , witch is known in traditional medicine .
The present study, which to our knowledge has never been studied of itis cardioprotectif effect against doxorubicin-induced cardiotoxicity and apoptotic activity before.
The analytical chemical study (identification tests, estimation of flavonoids content and total phenolic compound, The HPLCof the methanolic solution for this plant showed that :
Quantitative determination of total phenolics and total flavonoids was carried out using colorimetric methods. The total phenolic content was found to be73,14 mg of gallic acid equivalent per gram of extract, while the content of flavonoid show a value o f 21,61 ; 46,97 mg of quercetin , ritin equivalent per gram of extract. The antioxidant activity was evaluated in vitro with the use of free radical scavenging activity method by DPPH assay, Linoleic acid peroxidation inhibition, Estimation of total reducing power,Estimation of _OH. scavenging,
Lipid peroxidation inhibition assay, results indicated that dose depend % reduction againts the previous experiments mentioned the anticancer activity was evaluated by anti proliferation activity with MTT assay on tow
kind of cells line HepG2 and MDA . The proliferation of HepG2 , MDA was significantly decreased after 48h,72h incubation with PHSME at 450 ug/ml . also apoptosis and celle cycle activity were evaluated by flow cytometry methods .Apoptosis observed in Annexin VFTTC and propidium iodide (PI) staining of cells treated with PHSME for 48h in HepG2 MDA cells Morover PHSME arrest cell cycle progression in G1 phase ,The anticancer activity of PHSME has very potent anticancer activities ,which might be useful in liver and breast cancer .
The results indicate that PHSME extract exhibited significant protection against DOXO induced cardiac toxicity, where the pretreatment with the extract at a dose of 250 mg/kg for a 15 days resulted in a decrease in the plasma enzymatic cardiac injury (CPK, LDH and AST), reduction in cardiac cytosolic MDAand maintenance of cardiac cytosolic GSH level and catalase ,GST enzyms as compared to DOXO treated animals at a dose of 15 mg/kg
intraperitoneall. The biochemical study were confirmed with histological study; The results showed that doxorubicin alone produced evident histological alterations (edema, areas of necrosis, prominent inflammatory cells, Condensed Nuclei and other alterations) Its concluded that Methanolic extract of Phlomis samia treatment can protect against acute doxorubicin-induced cardiotoxicity. PHSME may be a useful candidate in the combination
therapy with doxorubicin to limit free-radical-mediated cardiac injury and can activate apoptosis in two kind of celle line (HepG2, MD
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/IHO7270.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10851 Effet d’extrait méthanolique de la plante médicinale Phlomis samia ()الخیاطةsur l’apoptose des cellules cancéreuses HepG2 et MDA MB 468 et sur la cardiotoxicité induite par la Doxorubicine. [texte imprimé] / Safia Ihoual ép Fethi, Auteur ; Nacira Abidli, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 179 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : le cancer Apoptose Phlomis samia la doxorubicine les radicaux libres cardiotoxicité l’anti oxydant apoptosis doxorubicin Ros cardiotoxicity antioxidant
السرطان الموت المبرمج للخلايا Phlomis samia(الخياطة) الجدور الحرة مضادات الاكسدة السمية القلبيةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The aim of this work was to investigate the effect of the aerial parts Méthanolic extract of Phlomis samia , witch is known in traditional medicine .
The present study, which to our knowledge has never been studied of itis cardioprotectif effect against doxorubicin-induced cardiotoxicity and apoptotic activity before.
The analytical chemical study (identification tests, estimation of flavonoids content and total phenolic compound, The HPLCof the methanolic solution for this plant showed that :
Quantitative determination of total phenolics and total flavonoids was carried out using colorimetric methods. The total phenolic content was found to be73,14 mg of gallic acid equivalent per gram of extract, while the content of flavonoid show a value o f 21,61 ; 46,97 mg of quercetin , ritin equivalent per gram of extract. The antioxidant activity was evaluated in vitro with the use of free radical scavenging activity method by DPPH assay, Linoleic acid peroxidation inhibition, Estimation of total reducing power,Estimation of _OH. scavenging,
Lipid peroxidation inhibition assay, results indicated that dose depend % reduction againts the previous experiments mentioned the anticancer activity was evaluated by anti proliferation activity with MTT assay on tow
kind of cells line HepG2 and MDA . The proliferation of HepG2 , MDA was significantly decreased after 48h,72h incubation with PHSME at 450 ug/ml . also apoptosis and celle cycle activity were evaluated by flow cytometry methods .Apoptosis observed in Annexin VFTTC and propidium iodide (PI) staining of cells treated with PHSME for 48h in HepG2 MDA cells Morover PHSME arrest cell cycle progression in G1 phase ,The anticancer activity of PHSME has very potent anticancer activities ,which might be useful in liver and breast cancer .
The results indicate that PHSME extract exhibited significant protection against DOXO induced cardiac toxicity, where the pretreatment with the extract at a dose of 250 mg/kg for a 15 days resulted in a decrease in the plasma enzymatic cardiac injury (CPK, LDH and AST), reduction in cardiac cytosolic MDAand maintenance of cardiac cytosolic GSH level and catalase ,GST enzyms as compared to DOXO treated animals at a dose of 15 mg/kg
intraperitoneall. The biochemical study were confirmed with histological study; The results showed that doxorubicin alone produced evident histological alterations (edema, areas of necrosis, prominent inflammatory cells, Condensed Nuclei and other alterations) Its concluded that Methanolic extract of Phlomis samia treatment can protect against acute doxorubicin-induced cardiotoxicity. PHSME may be a useful candidate in the combination
therapy with doxorubicin to limit free-radical-mediated cardiac injury and can activate apoptosis in two kind of celle line (HepG2, MD
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/IHO7270.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10851 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité IHO/7270 IHO/7270 Thèse Bibliothèque principale Thèses Disponible
Titre : Effet des extraits de la plante médicinale Ruta montana) (الفيجلsur la cardiotoxicité induite par la doxorubicine et sur la multi-drug résistances (MDR) des cellules cancéreuses ovarien (A2780) Type de document : texte imprimé Auteurs : Wahiba Kara Ali, Auteur ; N. Abidli, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 160 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Ruta montana Doxorubicine Cardiotoxicité Multi-drug résistance Stress oxydant Antioxydant Flavonoides Cardiotoxicity Multidrug resistance Oxidative
stress Antioxidant Flavonoids الدوكسوروبسين السمية القلبية المقاومة المتعددة للأدوية الإجهاد التاكسدي النشاط المضاد للأكسدة الفلافونويداتIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for the preventive effect of medicinal plant Ruta montana
extract on oxidative stress induced by doxorubicine (DOX) and its properties to reverse
doxorubicin resistance in ovarian cancer cell line (A2780). Organic extracts were obtained by
maceration with three solvents: petroleum ether, ethyl acetate and butanol. The yields were:
15,04%, 3,55% and 25,14%.respectively. A phytochemical screening in methanol extract
revealed that the plant contain the flavonoids, saponins, tannins, coumarins, reducing component
and the alkaloids. The quantitative study showed that the butanolic extract (BERM) has
significant amount of flavonoids (80.85±0.78mg EQ / g dry extract), followed by ethyl acetate
extract (AEERM) (15.27 ±0.62 EQ / g dry extract) and petroleum ether extract has the lowest
flavonoids content (PEERM) (9.71±1.13 mgEQ/g dry extract). When the total phenolic contents
was: 687.5, 595.83 and 146.33 mg EGA / g dry extract, in the BERM, AEERM and PEERM
respectively. The qualitative analysis of the three methanolic crude extract via thin layer
chromatography (TLC) has shown the presence of the anthocyanidin 3-glycosids, flavons et
flavonols glycosids (hétérosids), flavonols avec 3-OH libre, flavonons ou aurons, isoflavons, the
phénolic acids, Flavonols et 4-OH chalcons. The évaluation of antioxidant activity by the
reduction of free radical DPPH method showed that The highest DPPH radical scavenging
activity was observed with AEERM (IC50 = 0.126 ± 0.002 mg/ ml), followed by BERM (0.135
± 0.0006 mg/ ml) and PEERM (IC50 =0.185± 0.002 mg/ ml). The study of preventive effect of
AEERM on oxidative stress induced by DOX in wistar rats clearly show that DOX (15 mg/kg
i.p) induced animal cardiotoxicity characterized by an increase of cardiac enzyme activity
(CKMB, LDH, l’ALAT et l’ASAT) in serum, the increase in the concentration of
malondialdéhyde (MDA), antioxydant enzyme activity, catalase (CAT) and Glutathion- Stransférase (GST) and a decrease of cardiac cytosolic reduced glutathione (GSH) with a different
pathological changes in the heart. However pretreated animals with EAERM (100 mg/kg orally
during 15 days), showed a decrease of cardiac enzymes activities 24 %, 35, 69%, 21,25 % et
8,10 % respectively to CKMB, LDH, l’ALAT and l’ASAT. The preatretment with the extract
improved antioxidant status in the heart by a decrease in the cardiac MDA concentration by 29,7
% and activity of antioxydants enzymes by 17,91% et 29,78% respectively to CAT et la GST.
In addition, the pretreated rats by the extract showed an increase of GSH level (83,76 %)
compared with DOX-treated animals. Histological study shows that the extract resulted in a
reduction of severe cardiac damages observed in DOX-treated animals. The reversal of
multidrug resistance for DOX in human ovarian adenocarcinoma, cell lines (A2780 DX3) using
MTT assay showed that AEERM was very effective at reversing the resistant in dose dependent
manner with the Reversion Factor (RF =8.62, 05.77 and 02.30 respectively for the
concentrations 40, 10 and 2.5 mg/ml) followed by BERM (FR=05.07 and 02.43 for the
concentrations 40 and 10 mg/ml respectively), then the PEERM had the lowest reversal
resistance effect (RF betwen 1.01 to 1.08). The present study suggests that AEERM can
ameliorate the oxidative stress involved in the pathogenesis of doxorubicine- induced
cardiotoxicity and can reverse the multidrug resistance for DOX.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAR7158.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10435 Effet des extraits de la plante médicinale Ruta montana) (الفيجلsur la cardiotoxicité induite par la doxorubicine et sur la multi-drug résistances (MDR) des cellules cancéreuses ovarien (A2780) [texte imprimé] / Wahiba Kara Ali, Auteur ; N. Abidli, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 160 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Ruta montana Doxorubicine Cardiotoxicité Multi-drug résistance Stress oxydant Antioxydant Flavonoides Cardiotoxicity Multidrug resistance Oxidative
stress Antioxidant Flavonoids الدوكسوروبسين السمية القلبية المقاومة المتعددة للأدوية الإجهاد التاكسدي النشاط المضاد للأكسدة الفلافونويداتIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This study was conducted to search for the preventive effect of medicinal plant Ruta montana
extract on oxidative stress induced by doxorubicine (DOX) and its properties to reverse
doxorubicin resistance in ovarian cancer cell line (A2780). Organic extracts were obtained by
maceration with three solvents: petroleum ether, ethyl acetate and butanol. The yields were:
15,04%, 3,55% and 25,14%.respectively. A phytochemical screening in methanol extract
revealed that the plant contain the flavonoids, saponins, tannins, coumarins, reducing component
and the alkaloids. The quantitative study showed that the butanolic extract (BERM) has
significant amount of flavonoids (80.85±0.78mg EQ / g dry extract), followed by ethyl acetate
extract (AEERM) (15.27 ±0.62 EQ / g dry extract) and petroleum ether extract has the lowest
flavonoids content (PEERM) (9.71±1.13 mgEQ/g dry extract). When the total phenolic contents
was: 687.5, 595.83 and 146.33 mg EGA / g dry extract, in the BERM, AEERM and PEERM
respectively. The qualitative analysis of the three methanolic crude extract via thin layer
chromatography (TLC) has shown the presence of the anthocyanidin 3-glycosids, flavons et
flavonols glycosids (hétérosids), flavonols avec 3-OH libre, flavonons ou aurons, isoflavons, the
phénolic acids, Flavonols et 4-OH chalcons. The évaluation of antioxidant activity by the
reduction of free radical DPPH method showed that The highest DPPH radical scavenging
activity was observed with AEERM (IC50 = 0.126 ± 0.002 mg/ ml), followed by BERM (0.135
± 0.0006 mg/ ml) and PEERM (IC50 =0.185± 0.002 mg/ ml). The study of preventive effect of
AEERM on oxidative stress induced by DOX in wistar rats clearly show that DOX (15 mg/kg
i.p) induced animal cardiotoxicity characterized by an increase of cardiac enzyme activity
(CKMB, LDH, l’ALAT et l’ASAT) in serum, the increase in the concentration of
malondialdéhyde (MDA), antioxydant enzyme activity, catalase (CAT) and Glutathion- Stransférase (GST) and a decrease of cardiac cytosolic reduced glutathione (GSH) with a different
pathological changes in the heart. However pretreated animals with EAERM (100 mg/kg orally
during 15 days), showed a decrease of cardiac enzymes activities 24 %, 35, 69%, 21,25 % et
8,10 % respectively to CKMB, LDH, l’ALAT and l’ASAT. The preatretment with the extract
improved antioxidant status in the heart by a decrease in the cardiac MDA concentration by 29,7
% and activity of antioxydants enzymes by 17,91% et 29,78% respectively to CAT et la GST.
In addition, the pretreated rats by the extract showed an increase of GSH level (83,76 %)
compared with DOX-treated animals. Histological study shows that the extract resulted in a
reduction of severe cardiac damages observed in DOX-treated animals. The reversal of
multidrug resistance for DOX in human ovarian adenocarcinoma, cell lines (A2780 DX3) using
MTT assay showed that AEERM was very effective at reversing the resistant in dose dependent
manner with the Reversion Factor (RF =8.62, 05.77 and 02.30 respectively for the
concentrations 40, 10 and 2.5 mg/ml) followed by BERM (FR=05.07 and 02.43 for the
concentrations 40 and 10 mg/ml respectively), then the PEERM had the lowest reversal
resistance effect (RF betwen 1.01 to 1.08). The present study suggests that AEERM can
ameliorate the oxidative stress involved in the pathogenesis of doxorubicine- induced
cardiotoxicity and can reverse the multidrug resistance for DOX.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KAR7158.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10435 Exemplaires
Code-barres Cote Support Localisation Section Disponibilité aucun exemplaire
Titre : Effet préventif et curatif de l'extrait de la plante médicinale Crataegus oxyacantha L.sur le strss oxydant induit par la Doxorubicine Type de document : texte imprimé Auteurs : Univ. de Constantine, Éditeur scientifique Année de publication : 2009 Importance : 98 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Stress oxydant Doxorubicine Cardiotoxicité Carataegus oxyacantha L. . Flavonoides Activité antioxydante Effet préventif Effet curatif Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/BRA5445.pdf Permalink : index.php?lvl=notice_display&id=1361 Effet préventif et curatif de l'extrait de la plante médicinale Crataegus oxyacantha L.sur le strss oxydant induit par la Doxorubicine [texte imprimé] / Univ. de Constantine, Éditeur scientifique . - 2009 . - 98 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Stress oxydant Doxorubicine Cardiotoxicité Carataegus oxyacantha L. . Flavonoides Activité antioxydante Effet préventif Effet curatif Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/BRA5445.pdf Permalink : index.php?lvl=notice_display&id=1361 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BRA/5445 BRA/5445 Thèse Bibliothèque principale Thèses Disponible
