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Partager le résultat de cette recherche Interroger des sources externesEssais d'optimisation des conditions de cuisson et d'extraction des polyphénols de six légumes et évaluation des activités / Imène Laib
Titre : Essais d'optimisation des conditions de cuisson et d'extraction des polyphénols de six légumes et évaluation des activités : antioxydante et anticholinestérase. Type de document : texte imprimé Auteurs : Imène Laib, Auteur ; Malika Barkat, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 287 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Optimisation légume cuisson polyphénols totaux flavonoïdes activité antioxydante activité anticholinestérase Optimization vegetable cooking total polyphenolic flavonoids antioxidant activity anticholinesterase activity لتحسين الخضار الطبخ البوليفينول الكلي الفلافونويد النشاط المضاد للأكسدة نشاط مضادات
الكولينستريزIndex. décimale : 640 Industrie Alimentaire Résumé : This study focuses on the optimization of the cooking conditions and the extraction of polyphenols from six vegetables (potato, onion, spinach, aubergine carrot and pepper) and the study of the impact of the cooking method (boiling, steaming and microwaving) on these compounds and their biological activities (antioxidant activity and anticholinesterase activity).
To evaluate the quantity of phenolic compounds extracted from raw and cooked vegetables, a experiment design was realized. The factors chosen are (Temperature, time, power) for cooking and (solvent concentration, maceration time, ultrasonication and grinding) for polyphenol extraction . The study was initiated by Plackett-Burmann design to perform the screening of factors and to select the most influential on the Y response (total phenolic) and an optimization by exploiting central composite design. Determination of total polyphenols, flavonoids and LC-MS / MS analysis were performed to monitor the variation of phenolic compounds after cooking. In addition, in this work, we anlyzed antioxidant and anticholinesterase properties of the phenolic compounds of the six raw and cooked vegetables.
The results obtained for the six vegetables are different, boiling in water and steam cooking methods have reduced the levels of total polyphenols and flavonoids of the six vegetables. While microwave cooking had different effects on the six vegetables, it had a negative effect on the total polyphenol and flavonoid levels of the potato, spinach, carrot and pepper.
However, a positive effect was recorded on the total polyphenols and flavonoids of eggplant. Retention of these compounds has been observed in onions cooked by microwave. The impact of cooking on antioxidant activity varies from one vegetable to another. Cooking caused a decrease in the antioxidant activity of potatoes, onions, carrots, peppers and steamed spinach.
A positive effect of cooking was recorded on the antioxidant potential of spinach (cooked in water and by microwave) and eggplant. All three cooking methods showed negative effects on the anticholinesterase activity of potato, spinach and pepper. However, an increase in this activity was recorded for onion, eggplant and carrot. Cooking in water proved to be the worst method for preserving the polyphenols from cooked vegetables and their activities (antioxidant activity and anticholinesterase). The losses of polyphenols are due to their increased solubility in water. However, microwave cooking is suggested as the best method to preserve the polyphenol levels, the antioxidant and anticholinesterase activities of the studied vegetables.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/LAI7279.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10894 Essais d'optimisation des conditions de cuisson et d'extraction des polyphénols de six légumes et évaluation des activités : antioxydante et anticholinestérase. [texte imprimé] / Imène Laib, Auteur ; Malika Barkat, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 287 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Optimisation légume cuisson polyphénols totaux flavonoïdes activité antioxydante activité anticholinestérase Optimization vegetable cooking total polyphenolic flavonoids antioxidant activity anticholinesterase activity لتحسين الخضار الطبخ البوليفينول الكلي الفلافونويد النشاط المضاد للأكسدة نشاط مضادات
الكولينستريزIndex. décimale : 640 Industrie Alimentaire Résumé : This study focuses on the optimization of the cooking conditions and the extraction of polyphenols from six vegetables (potato, onion, spinach, aubergine carrot and pepper) and the study of the impact of the cooking method (boiling, steaming and microwaving) on these compounds and their biological activities (antioxidant activity and anticholinesterase activity).
To evaluate the quantity of phenolic compounds extracted from raw and cooked vegetables, a experiment design was realized. The factors chosen are (Temperature, time, power) for cooking and (solvent concentration, maceration time, ultrasonication and grinding) for polyphenol extraction . The study was initiated by Plackett-Burmann design to perform the screening of factors and to select the most influential on the Y response (total phenolic) and an optimization by exploiting central composite design. Determination of total polyphenols, flavonoids and LC-MS / MS analysis were performed to monitor the variation of phenolic compounds after cooking. In addition, in this work, we anlyzed antioxidant and anticholinesterase properties of the phenolic compounds of the six raw and cooked vegetables.
The results obtained for the six vegetables are different, boiling in water and steam cooking methods have reduced the levels of total polyphenols and flavonoids of the six vegetables. While microwave cooking had different effects on the six vegetables, it had a negative effect on the total polyphenol and flavonoid levels of the potato, spinach, carrot and pepper.
However, a positive effect was recorded on the total polyphenols and flavonoids of eggplant. Retention of these compounds has been observed in onions cooked by microwave. The impact of cooking on antioxidant activity varies from one vegetable to another. Cooking caused a decrease in the antioxidant activity of potatoes, onions, carrots, peppers and steamed spinach.
A positive effect of cooking was recorded on the antioxidant potential of spinach (cooked in water and by microwave) and eggplant. All three cooking methods showed negative effects on the anticholinesterase activity of potato, spinach and pepper. However, an increase in this activity was recorded for onion, eggplant and carrot. Cooking in water proved to be the worst method for preserving the polyphenols from cooked vegetables and their activities (antioxidant activity and anticholinesterase). The losses of polyphenols are due to their increased solubility in water. However, microwave cooking is suggested as the best method to preserve the polyphenol levels, the antioxidant and anticholinesterase activities of the studied vegetables.
Diplôme : Doctorat en sciences En ligne : ../theses/agronomie/LAI7279.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10894 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAI/7279 LAI/7279 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude comparative du profil chimique et de l’activité antioxydante de plusieurs propolis de l’Est algérien et investigation phytochimique de la propolis la plus active. Type de document : texte imprimé Auteurs : Amina Bouaroura ép Redjem, Auteur ; Narimane Segueni, Directeur de thèse Mention d'édition : 25/06/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 152 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie pharmaceutique:Analyse physicochimique, contrôle de la qualité et synthèse de substances bioactives Propolis étude comparative activité antioxydante activité anticholinestérase polyphénols flavonoïdes LC-MS comparative study antioxidant activitie anticholinesterase activitie phenolics flavonoids العكبر المضاد للأكسدة المضاد للكولينستراز جهاز الكروماتوغرافي السائلة المقترن بمقياس الطيف الكتلي الفلافونويد عديدات الفينول Index. décimale : 540 Chimie et sciences connexes Résumé :
Our study is interested in the investigation of the chemical composition and the biological activities of Algerian propolis. Due to its several biological activities, propolis has been extensively used in folk medicine. In the first part of our study we examined several propolis harvested from north of Algeria in order to compare their antioxydant activity, In addition, the total phenolic and flavonoid contents were evaluated. Propolis of Constantine exhibited the highest antioxydant activity. This result encouraged us to study the anticholinesterase activity of the extracts of this propolis. This first part of our work allowed us to make a first classification of the propolis studied and to select the most active extracts on which we performed an analysis by liquid chromatography coupled to mass spectrophotometry LC-MS for the purpose of studying their chemical profile. The ethyl acetate extract of propolis of Constantine was the most active extract. This extract has the highest levels of caffeic acid, p-coumaric acid, naringenin and kaempferol. Our results suggest that the antioxydant effect is probably due to the presence of these compounds. In the second part of our study we investigated the chemical composition of Algerian propolis. The separation and purification of propolis extracts lead to the identification of 8 compounds, five flavonoids (tectochryin, pinocembrine, pinobksin-3-acetate, chrysin, galangin), one diterpene (cupressic acid) and two derivatives of caffeic acid (3-methyl-3-butenyl (E) -Caffeate and 2-methyl-2 -butenyl (E) -Caffeate). Their structures has been
established by spectral analysis including NMR mono (NMR-1H, NMR-13C) and bidimensionelle spectra (HSQC, HMBC) and impact electronic mass spectra.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7640.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11474 Etude comparative du profil chimique et de l’activité antioxydante de plusieurs propolis de l’Est algérien et investigation phytochimique de la propolis la plus active. [texte imprimé] / Amina Bouaroura ép Redjem, Auteur ; Narimane Segueni, Directeur de thèse . - 25/06/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 152 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie pharmaceutique:Analyse physicochimique, contrôle de la qualité et synthèse de substances bioactives Propolis étude comparative activité antioxydante activité anticholinestérase polyphénols flavonoïdes LC-MS comparative study antioxidant activitie anticholinesterase activitie phenolics flavonoids العكبر المضاد للأكسدة المضاد للكولينستراز جهاز الكروماتوغرافي السائلة المقترن بمقياس الطيف الكتلي الفلافونويد عديدات الفينول Index. décimale : 540 Chimie et sciences connexes Résumé :
Our study is interested in the investigation of the chemical composition and the biological activities of Algerian propolis. Due to its several biological activities, propolis has been extensively used in folk medicine. In the first part of our study we examined several propolis harvested from north of Algeria in order to compare their antioxydant activity, In addition, the total phenolic and flavonoid contents were evaluated. Propolis of Constantine exhibited the highest antioxydant activity. This result encouraged us to study the anticholinesterase activity of the extracts of this propolis. This first part of our work allowed us to make a first classification of the propolis studied and to select the most active extracts on which we performed an analysis by liquid chromatography coupled to mass spectrophotometry LC-MS for the purpose of studying their chemical profile. The ethyl acetate extract of propolis of Constantine was the most active extract. This extract has the highest levels of caffeic acid, p-coumaric acid, naringenin and kaempferol. Our results suggest that the antioxydant effect is probably due to the presence of these compounds. In the second part of our study we investigated the chemical composition of Algerian propolis. The separation and purification of propolis extracts lead to the identification of 8 compounds, five flavonoids (tectochryin, pinocembrine, pinobksin-3-acetate, chrysin, galangin), one diterpene (cupressic acid) and two derivatives of caffeic acid (3-methyl-3-butenyl (E) -Caffeate and 2-methyl-2 -butenyl (E) -Caffeate). Their structures has been
established by spectral analysis including NMR mono (NMR-1H, NMR-13C) and bidimensionelle spectra (HSQC, HMBC) and impact electronic mass spectra.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7640.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11474 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7640 BOU/7640 Thèse Bibliothèque principale Thèses Disponible
Titre : Composants et potentiel biologique de Cotula anthemoides L et Pentzia monodiana MAIRE. (Asteraceae). Type de document : texte imprimé Auteurs : Wafa Tadrent ép Laouadi, Auteur ; Zahia Kabouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 233 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Cotula anthemoides L Pentzia monodiana Maire Asteraceae lignanes flavonoïdes sesquiterpènes lactones activité antioxydante activité anticholinesterase activité
antityrosinase sesquiterpenes lactones antioxidant activity anticholinesterase activity antityrosinase activity فلافونيدات lignanesضد الأكسدة ضد الكولينستراز و ضد التيروزينازIndex. décimale : 540 Chimie et sciences connexes Résumé : The present work concerns the phytochemical and biological studies of areal parts of two Algerian plants belonging to the Asteraceae family: Cotula anthemoides L. and Pentzia monodiana Maire. This study led to the isolation and identification of fifteen compounds of Cotula anthemoides and eight compounds of Pentzia monodiana including five phenolic acids, nine flavonoids from which one is new, three lignans, one sesquiterpene lactone, two glycosyl phenolics, a glycosyl acid, a diterpene and an nucleoside. The structural determination of the isolated compounds was established by the use of 1D and 2D NMR spectroscopic techniques (1H, 13C, COSY, HSQC, HMBC and ROESY), by mass spectrometry (ESI-MS and HRESI-MS) and by comparison with the literature data. The evaluation of the antioxidant activity by 4 methods (DPPH, ABTS, CUPRAC and chelating by ferrous ions) of the various extracts, showed a good activity of extracts from P. monodiana compared to those of C. anthemoides. Furthermore, extracts of C. anthemoides showed an interesting anticholinesterase activity. However, both of lignanes, flavonol and flavones substituted in C-3, exhibited better antityrosinase and antiradical activities than some flavones only.
Diplôme : Doctorat En ligne : ../theses/chimie/TAD7353.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11002 Composants et potentiel biologique de Cotula anthemoides L et Pentzia monodiana MAIRE. (Asteraceae). [texte imprimé] / Wafa Tadrent ép Laouadi, Auteur ; Zahia Kabouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 233 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Cotula anthemoides L Pentzia monodiana Maire Asteraceae lignanes flavonoïdes sesquiterpènes lactones activité antioxydante activité anticholinesterase activité
antityrosinase sesquiterpenes lactones antioxidant activity anticholinesterase activity antityrosinase activity فلافونيدات lignanesضد الأكسدة ضد الكولينستراز و ضد التيروزينازIndex. décimale : 540 Chimie et sciences connexes Résumé : The present work concerns the phytochemical and biological studies of areal parts of two Algerian plants belonging to the Asteraceae family: Cotula anthemoides L. and Pentzia monodiana Maire. This study led to the isolation and identification of fifteen compounds of Cotula anthemoides and eight compounds of Pentzia monodiana including five phenolic acids, nine flavonoids from which one is new, three lignans, one sesquiterpene lactone, two glycosyl phenolics, a glycosyl acid, a diterpene and an nucleoside. The structural determination of the isolated compounds was established by the use of 1D and 2D NMR spectroscopic techniques (1H, 13C, COSY, HSQC, HMBC and ROESY), by mass spectrometry (ESI-MS and HRESI-MS) and by comparison with the literature data. The evaluation of the antioxidant activity by 4 methods (DPPH, ABTS, CUPRAC and chelating by ferrous ions) of the various extracts, showed a good activity of extracts from P. monodiana compared to those of C. anthemoides. Furthermore, extracts of C. anthemoides showed an interesting anticholinesterase activity. However, both of lignanes, flavonol and flavones substituted in C-3, exhibited better antityrosinase and antiradical activities than some flavones only.
Diplôme : Doctorat En ligne : ../theses/chimie/TAD7353.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11002 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité TAD/7353 TAD/7353 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique de deux espèces Crataegus azarolus L. (Rosaceae) et Dioscorea communis L. (Dioscoreaceae). Type de document : texte imprimé Auteurs : Amina Boudjada, Auteur ; Salah Rhouati, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 137 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Crataegus azarolus Dioscorea communis flavonoides phénanthrènes dihydrophénanthrènes activité antioxydante activité anticholinesterase phenanthrenes dihydrophenanthrenes antioxidant activity anticholinesterase activity Index. décimale : 540 Chimie et sciences connexes Résumé : This work is the subject of a phytochemical and biological study of two medicinal plants belonging to two different families. 1. Crataegus azarolus (L.), belonging to Rosaceae family:
After maceration of the aerial parts of the plant Crataegus azarolus in a methanolic solution and extraction with solvents of increasing polarity, three extracts are obtained. The extracts are separated using chromatographic methods of separation and purification, to lead finally to pure compounds. Then, the GC-MS analysis of a fraction of the CHCl3 extract allowed us to identify five volatile compounds for the first time from this species by comparing their mass spectra with the literature (NIST database). From the AcOEt extract five compounds were isolated, where two acetylated flavonoid glycosids were identified for the first time from the species. From the n-BuOH extract, three flavonoid glycosides are separated by CC and TLC. Their structures are elucidated through spectroscopic analysis methods. The antioxidant activity of the extracts and compounds is evaluated using five different methods (DPPH, ABTS, CUPRAC, FRAP and β-carotene). 2. Dioscorea communis (L.) Caddick & Wilkin, belonging to Dioscoreaceae family: Also, after maceration of the rhizomes of Dioscorea communis in diethyl ether solvent. The Et2O extract obtained was separated by chromatographic techniques (CC, TLC) to finally result in several compounds, seven of which are identified by spectroscopic methods of analyzes (MS, IR, UV, 1D and 2D NMR), where three of them are identified for the first time from this species. Et2O, AcOEt Extracts and some isolated compounds have shown an antioxidant activity by using five different methods (DPPH, ABTS, CUPRAC, FRAP and β-Carrotene), also these extracts and compounds are tested for the first time against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. This study confirmed that the presence of OH groups on the phenanthrene skeleton is essential for its antioxidant and anticholinesterase activities.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7346.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10996 Etude phytochimique de deux espèces Crataegus azarolus L. (Rosaceae) et Dioscorea communis L. (Dioscoreaceae). [texte imprimé] / Amina Boudjada, Auteur ; Salah Rhouati, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 137 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Crataegus azarolus Dioscorea communis flavonoides phénanthrènes dihydrophénanthrènes activité antioxydante activité anticholinesterase phenanthrenes dihydrophenanthrenes antioxidant activity anticholinesterase activity Index. décimale : 540 Chimie et sciences connexes Résumé : This work is the subject of a phytochemical and biological study of two medicinal plants belonging to two different families. 1. Crataegus azarolus (L.), belonging to Rosaceae family:
After maceration of the aerial parts of the plant Crataegus azarolus in a methanolic solution and extraction with solvents of increasing polarity, three extracts are obtained. The extracts are separated using chromatographic methods of separation and purification, to lead finally to pure compounds. Then, the GC-MS analysis of a fraction of the CHCl3 extract allowed us to identify five volatile compounds for the first time from this species by comparing their mass spectra with the literature (NIST database). From the AcOEt extract five compounds were isolated, where two acetylated flavonoid glycosids were identified for the first time from the species. From the n-BuOH extract, three flavonoid glycosides are separated by CC and TLC. Their structures are elucidated through spectroscopic analysis methods. The antioxidant activity of the extracts and compounds is evaluated using five different methods (DPPH, ABTS, CUPRAC, FRAP and β-carotene). 2. Dioscorea communis (L.) Caddick & Wilkin, belonging to Dioscoreaceae family: Also, after maceration of the rhizomes of Dioscorea communis in diethyl ether solvent. The Et2O extract obtained was separated by chromatographic techniques (CC, TLC) to finally result in several compounds, seven of which are identified by spectroscopic methods of analyzes (MS, IR, UV, 1D and 2D NMR), where three of them are identified for the first time from this species. Et2O, AcOEt Extracts and some isolated compounds have shown an antioxidant activity by using five different methods (DPPH, ABTS, CUPRAC, FRAP and β-Carrotene), also these extracts and compounds are tested for the first time against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. This study confirmed that the presence of OH groups on the phenanthrene skeleton is essential for its antioxidant and anticholinesterase activities.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7346.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10996 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7346 BOU/7346 Thèse Bibliothèque principale Thèses Disponible
