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Partager le résultat de cette recherche Interroger des sources externesEtude phytochimique et evaluation biologique de l’espèce senecio delphinifolius Vahl / Soukaina Tidjani
Titre : Etude phytochimique et evaluation biologique de l’espèce senecio delphinifolius Vahl Type de document : texte imprimé Auteurs : Soukaina Tidjani, Auteur ; Salah Rhouati, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 187 f. Format : 30 cm. Langues : Français (fre) Tags : Asteraceae Senecio delphinifolius Alcaloïdes pyrrolizidiniques Flavonoïdes Furanoeremophilane quinone CGMS Chromatographie activité antioxydante cytotoxicité activité antibactérienne Senecio delphinifolius Vahl Pyrrolizidine alkaloids Flavonoïds GCMS Chromatography antibacterial Antioxidant Cytotoxic ??????? ??????????? ??????????? ???????? ??????? ?????????????? ????? ?????? ???????? ???????? ?????? (SM/CG) ?????? ????? ??????? ?????? ????? ????????? Index. décimale : 540 Chimie et sciences connexes Résumé : Although Senecio species are known as sources of potentially toxic pyrrolizidine alkaloids (PAs), some species of this genus are traditionally used as remedies. In this work the evaluation of biological activities and the analysis of PAs of Algerian specimens of Senecio delphinifolius Vahl (its vernacular name in Algeria is ""Hachichetettolba"") are reported. Thus it was possible to identify six APs by GC and GC-MS analysis: (1) senecionine (2) seneciphylline (3) integerrimine (4) senkirkine (5) dehydrosenkirkine and (6) neosenkirkine, this technique is frequently used for complex mixtures of such compounds. The species S.
delphinifolius, contains 140 ppm of PAs belong to the toxic series (1, 2-unsaturation in the pyrrolizidine cycle and macrocyclic diester), the use of S. delphinifolius should be discouraged in traditional medicine.
Our research has also led to the isolation by conventional purification techniques of three compounds from the n-butanol extract of the specie S. delphinifolius Vahl two of them are glycosylated flavonols, (7) Quercetine 3-O-α-(rhamnopyrannosyl (1-6) glucopyrannose) [rutine], (8) Kaempferol 3,7-di-O-α-Lrhamnopyranoside [Kaempferitin], the third is a derivative of 1, 4-benzoquinone that are common in the
genus Senecio (9) 2, 5 Cyclohexadiene-1-acetic acid, 1-methyl-4-oxo, methyl ester. A fourth product was isolated from the dichloromethane extract, the structure is being identified. The structures of the isolated compounds were elucidated through the use of NMR techniques 1D and 2D, mass spectroscopy, UV spectroscopy and acid hydrolysis. (10) Chlorogenic acid was identified by co-chromatography with standards.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/TID7016.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10395 Etude phytochimique et evaluation biologique de l’espèce senecio delphinifolius Vahl [texte imprimé] / Soukaina Tidjani, Auteur ; Salah Rhouati, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2016 . - 187 f. ; 30 cm.
Langues : Français (fre)
Tags : Asteraceae Senecio delphinifolius Alcaloïdes pyrrolizidiniques Flavonoïdes Furanoeremophilane quinone CGMS Chromatographie activité antioxydante cytotoxicité activité antibactérienne Senecio delphinifolius Vahl Pyrrolizidine alkaloids Flavonoïds GCMS Chromatography antibacterial Antioxidant Cytotoxic ??????? ??????????? ??????????? ???????? ??????? ?????????????? ????? ?????? ???????? ???????? ?????? (SM/CG) ?????? ????? ??????? ?????? ????? ????????? Index. décimale : 540 Chimie et sciences connexes Résumé : Although Senecio species are known as sources of potentially toxic pyrrolizidine alkaloids (PAs), some species of this genus are traditionally used as remedies. In this work the evaluation of biological activities and the analysis of PAs of Algerian specimens of Senecio delphinifolius Vahl (its vernacular name in Algeria is ""Hachichetettolba"") are reported. Thus it was possible to identify six APs by GC and GC-MS analysis: (1) senecionine (2) seneciphylline (3) integerrimine (4) senkirkine (5) dehydrosenkirkine and (6) neosenkirkine, this technique is frequently used for complex mixtures of such compounds. The species S.
delphinifolius, contains 140 ppm of PAs belong to the toxic series (1, 2-unsaturation in the pyrrolizidine cycle and macrocyclic diester), the use of S. delphinifolius should be discouraged in traditional medicine.
Our research has also led to the isolation by conventional purification techniques of three compounds from the n-butanol extract of the specie S. delphinifolius Vahl two of them are glycosylated flavonols, (7) Quercetine 3-O-α-(rhamnopyrannosyl (1-6) glucopyrannose) [rutine], (8) Kaempferol 3,7-di-O-α-Lrhamnopyranoside [Kaempferitin], the third is a derivative of 1, 4-benzoquinone that are common in the
genus Senecio (9) 2, 5 Cyclohexadiene-1-acetic acid, 1-methyl-4-oxo, methyl ester. A fourth product was isolated from the dichloromethane extract, the structure is being identified. The structures of the isolated compounds were elucidated through the use of NMR techniques 1D and 2D, mass spectroscopy, UV spectroscopy and acid hydrolysis. (10) Chlorogenic acid was identified by co-chromatography with standards.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/TID7016.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10395 Exemplaires
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Titre : Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) Type de document : texte imprimé Auteurs : Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 353 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Tags : Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum ?????? ????????? ???????? ???? ??????? ???? ????????? ???? ???????? ???? ?????????????" Index. décimale : 540 Chimie et sciences connexes Résumé : Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LAR7157.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10430 Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) [texte imprimé] / Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 353 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Tags : Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum ?????? ????????? ???????? ???? ??????? ???? ????????? ???? ???????? ???? ?????????????" Index. décimale : 540 Chimie et sciences connexes Résumé : Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LAR7157.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10430 Exemplaires
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Titre : Étude phytochimique et évaluation des activités antioxydante, antibactérienne et cytotoxique de deux plantes du genre Phagnalon (Asteraceae) Type de document : texte imprimé Auteurs : Hanene Cherchar ép Saadi, Auteur ; Djemaa Berrehal, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 190 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Asteraceae Phagnalon saxatile Phagnalon sordidum activité antioxydante activité antibactérienne cytotoxicité antioxidant activity antibacterial activity cytotoxicity ?????? ????? ??????? ?????? ????? ????????? ?????? ??????? Index. décimale : 540 Chimie et sciences connexes Résumé : Our phytochemical and biological works concerned the aerial parts of two species belonging to Asteraeae family : Phagnalon saxatile (L) Cass. and Phagnalon sordidum L.
Several chromatographic methods allowed us to isolate ten products from the n-butanol and ethyl acetate extracts of Phagnalon saxatile from which one is new, and twenty one products from Phagnalon sordidum.
Structures were determined by different spectral methods: nuclear magnetic resonance with different applications (NMR 1H, NMR 13C, COSY, HSQC and HMBC), the mass spectrometry, UV, acid hydrolysis as well as comparison with literature data.
4 methods were used to evaluate the antioxidant activity of Phagnalon sordidum and Phagnalon saxatile. The latter showed a good activity by the use of 4 methods: DPPH, ABTS, CUPRAC and superoxide radical scavenging activity by alkaline DMSO whereas Phagnalon sordidum exhibited a moderate activity with DPPH and CUPRAC tests.
Furthermore, petroleum ether and chloroform extracts from Phagnalon saxatile showed moderate antibacterial activity against the tested strains. The isolated products (1-O- β-D-glucopyranosyl-1,4-dihydroxy-2-((E) 2-oxo-3-butenyl) benzene, 1-O-β-glucopyranosyl- 2(3’,3’-diméthylallyl) hydroquinone and Potassium 4-hydroxy-3-methoxybenzoique acide
methyl ester-5-sulfate) from Phagnalon saxatile showed a moderate cytotoxic activity by the use of MTT method.
Diplôme : Doctorat En ligne : ../theses/chimie/CHE7249.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10841 Étude phytochimique et évaluation des activités antioxydante, antibactérienne et cytotoxique de deux plantes du genre Phagnalon (Asteraceae) [texte imprimé] / Hanene Cherchar ép Saadi, Auteur ; Djemaa Berrehal, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 190 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Asteraceae Phagnalon saxatile Phagnalon sordidum activité antioxydante activité antibactérienne cytotoxicité antioxidant activity antibacterial activity cytotoxicity ?????? ????? ??????? ?????? ????? ????????? ?????? ??????? Index. décimale : 540 Chimie et sciences connexes Résumé : Our phytochemical and biological works concerned the aerial parts of two species belonging to Asteraeae family : Phagnalon saxatile (L) Cass. and Phagnalon sordidum L.
Several chromatographic methods allowed us to isolate ten products from the n-butanol and ethyl acetate extracts of Phagnalon saxatile from which one is new, and twenty one products from Phagnalon sordidum.
Structures were determined by different spectral methods: nuclear magnetic resonance with different applications (NMR 1H, NMR 13C, COSY, HSQC and HMBC), the mass spectrometry, UV, acid hydrolysis as well as comparison with literature data.
4 methods were used to evaluate the antioxidant activity of Phagnalon sordidum and Phagnalon saxatile. The latter showed a good activity by the use of 4 methods: DPPH, ABTS, CUPRAC and superoxide radical scavenging activity by alkaline DMSO whereas Phagnalon sordidum exhibited a moderate activity with DPPH and CUPRAC tests.
Furthermore, petroleum ether and chloroform extracts from Phagnalon saxatile showed moderate antibacterial activity against the tested strains. The isolated products (1-O- β-D-glucopyranosyl-1,4-dihydroxy-2-((E) 2-oxo-3-butenyl) benzene, 1-O-β-glucopyranosyl- 2(3’,3’-diméthylallyl) hydroquinone and Potassium 4-hydroxy-3-methoxybenzoique acide
methyl ester-5-sulfate) from Phagnalon saxatile showed a moderate cytotoxic activity by the use of MTT method.
Diplôme : Doctorat En ligne : ../theses/chimie/CHE7249.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10841 Exemplaires
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Titre : Étude phytochimique et biologique de trois espèces Algériennes : Calendula stellata Cav. (Asteraceae), Scabiosa stellata L. (Caprifoliaceae) et Salvia barrelieri Etl. (Lamiaceae). Type de document : texte imprimé Auteurs : Meryem Lehbili, Auteur ; Ahmed Kabouche, Directeur de thèse ; Abdulmagid Alabdul Magid, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 375 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Calendula stellata Scabiosa stellata Salvia barrelieri saponosides iridoïdes triterpenoïdes flavonoïdes activité antioxydante activité antimicrobienne cytotoxicité saponosids iridoids triterpenes flavonoids antioxidant activity antimicrobial activity cytotoxicity ???????? ?????????? ????? ??????? ????? ????????? ???? ????? Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical study of three species: Calendula stellata, Scabiosa stellata and Salvia barrelieri led to the isolation of 55 compounds, from which 16 were new, by the use of different chromatographic methods of separation and purification on solid or liquid support.
The isolated compounds were distributed through 27 saponosids, 7 triterpenes, 11 flavonoids, 7 iridoids and 2 chlorogenic acid derivatives. Two mono-iridoids and two flavonoids showed an important antiradical activity (IC50:7-16 µM). Fourteen compounds exhibited a good antibacterial activity (MIC: 15- 125 µg/mL). The new compounds showed a low to moderate cytotoxic activity against all tested cell lines, except for the monodesmoside, obtained from Scabiosa stellata (Scabiostellatoside F) which exhibited a good antiproliferative effect against HT1080 (IC50 = 12 µM).
Diplôme : Doctorat En ligne : ../theses/chimie/LEH7306.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10921 Étude phytochimique et biologique de trois espèces Algériennes : Calendula stellata Cav. (Asteraceae), Scabiosa stellata L. (Caprifoliaceae) et Salvia barrelieri Etl. (Lamiaceae). [texte imprimé] / Meryem Lehbili, Auteur ; Ahmed Kabouche, Directeur de thèse ; Abdulmagid Alabdul Magid, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 375 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Calendula stellata Scabiosa stellata Salvia barrelieri saponosides iridoïdes triterpenoïdes flavonoïdes activité antioxydante activité antimicrobienne cytotoxicité saponosids iridoids triterpenes flavonoids antioxidant activity antimicrobial activity cytotoxicity ???????? ?????????? ????? ??????? ????? ????????? ???? ????? Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical study of three species: Calendula stellata, Scabiosa stellata and Salvia barrelieri led to the isolation of 55 compounds, from which 16 were new, by the use of different chromatographic methods of separation and purification on solid or liquid support.
The isolated compounds were distributed through 27 saponosids, 7 triterpenes, 11 flavonoids, 7 iridoids and 2 chlorogenic acid derivatives. Two mono-iridoids and two flavonoids showed an important antiradical activity (IC50:7-16 µM). Fourteen compounds exhibited a good antibacterial activity (MIC: 15- 125 µg/mL). The new compounds showed a low to moderate cytotoxic activity against all tested cell lines, except for the monodesmoside, obtained from Scabiosa stellata (Scabiostellatoside F) which exhibited a good antiproliferative effect against HT1080 (IC50 = 12 µM).
Diplôme : Doctorat En ligne : ../theses/chimie/LEH7306.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10921 Exemplaires
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Titre : Synthèse de composés hétérocycliques à visée anticancèreuse Type de document : texte imprimé Auteurs : Nora Chouha, Auteur ; Taoues Boumoud, Auteur ; Laurent Desaubry, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 155 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Triarylimidazoles spirooxindoles acide citrique BF3.OEt2 iminobenzimidazole fluorizoline oncogène KRAS cytotoxicité leucémie citric acid KRAS oncogene cytotoxicity leukemia Index. décimale : 540 Chimie et sciences connexes Résumé : Our work focuses on the development of new synthetic approaches to anticancer compounds. This work was carried out at the University of Constantine and Strasbourg, under Prof. Taoues Boumoud and Dr. Laurent Désaubry’s supervision. Triarylimidazoles and spirooxindoles are heterocycles found among several important anti-cancer agents. In order to facilitate the pharmacochemical exploration of these compounds, improving their synthesis was aimed. Novel catalytic ways were developped for the synthesis of triarylimidazoles and spiro[chroméne-oxindoles] with good yields via multicomponent reactions in the presence of citric acid and BF3.OEt2 as catalysts respectively. Two articles demonstarting the catalytic effects of the former catalysts in the synthesis of benzimidazoles and spirooxindole derivatives were published. Original work carried out in Strasbourg has demonstrated the cytotoxic properties of an iminobenzimidazole on cancerous lines. Analogues of this compound, were prepered, to determine the structural requirements for this cytotoxicity. An article describing these works was published inThe Open Medicinal Chemistry Journal. Another cytotoxic agent “fluorizoline” and analogues thereof were also prepared. Two first articles demonstrating the inhibition of KRAS oncogene by this compound and its activity in models of chronic lymphocytic leukemia were also published.
Diplôme : Doctorat En ligne : ../theses/chimie/CHO7370.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11019 Synthèse de composés hétérocycliques à visée anticancèreuse [texte imprimé] / Nora Chouha, Auteur ; Taoues Boumoud, Auteur ; Laurent Desaubry, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 155 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Triarylimidazoles spirooxindoles acide citrique BF3.OEt2 iminobenzimidazole fluorizoline oncogène KRAS cytotoxicité leucémie citric acid KRAS oncogene cytotoxicity leukemia Index. décimale : 540 Chimie et sciences connexes Résumé : Our work focuses on the development of new synthetic approaches to anticancer compounds. This work was carried out at the University of Constantine and Strasbourg, under Prof. Taoues Boumoud and Dr. Laurent Désaubry’s supervision. Triarylimidazoles and spirooxindoles are heterocycles found among several important anti-cancer agents. In order to facilitate the pharmacochemical exploration of these compounds, improving their synthesis was aimed. Novel catalytic ways were developped for the synthesis of triarylimidazoles and spiro[chroméne-oxindoles] with good yields via multicomponent reactions in the presence of citric acid and BF3.OEt2 as catalysts respectively. Two articles demonstarting the catalytic effects of the former catalysts in the synthesis of benzimidazoles and spirooxindole derivatives were published. Original work carried out in Strasbourg has demonstrated the cytotoxic properties of an iminobenzimidazole on cancerous lines. Analogues of this compound, were prepered, to determine the structural requirements for this cytotoxicity. An article describing these works was published inThe Open Medicinal Chemistry Journal. Another cytotoxic agent “fluorizoline” and analogues thereof were also prepared. Two first articles demonstrating the inhibition of KRAS oncogene by this compound and its activity in models of chronic lymphocytic leukemia were also published.
Diplôme : Doctorat En ligne : ../theses/chimie/CHO7370.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11019 Exemplaires
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