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Partager le résultat de cette recherche Interroger des sources externesتحدید مركبات الأیض الثانوي وَ الدراسة البیولوجیة لنباتات عطریة من جنسي Salvia وَ Stachys / هشام لكحل
Titre : تحدید مركبات الأیض الثانوي وَ الدراسة البیولوجیة لنباتات عطریة من جنسي Salvia وَ Stachys Type de document : texte imprimé Auteurs : هشام لكحل, Auteur ; أحمد كعبوش, Directeur de thèse Année de publication : 2014 Importance : 245 ورقة. Format : 30 سم. Note générale : مذكرة دكتوراه العلوم
2نسخ موجودة في المكتبة المركزيةLangues : Arabe (ara) Catégories : Arabe
الكيمياء و العلوم ذات العلاقةTags : triterpènes saponoside phenylpropanoide flavonoides activité antioxydante antibacterienne Stachys Salvia Lamiaceae triterpenes saponin flavonoids phenylpropanoid antioxidant activity antibacterial تربین ثلاثي صابونین فلافونویدات إسترفینیل بروبانوید فعالیة مضادة للأكسدة فعالیة مضادة للبكتیریا Index. décimale : 540 الكيمياء و العلوم ذات العلاقة Résumé : In this work, we conducted the phytochemical study and evaluated the antibacterial and antioxidant activities of plants grown in the Eastern Algerian belonging to the Lamiaceae family : Salvia argentea var. aurasiaca (Pomel) Batt. et Trab., Stachys ocymastrum (L.) Briq. et Salvia officinalis L..
The phytochemical study of the species Salvia argentea var. aurasiaca (Pomel) Batt. et Trab resulted in the isolation of 19 compounds of which 12 were described for the first time in the plant kingdom, The isolated compounds belong to six classes of secondary metabolites:
- 12 triterpenes
- 1 saponin
- 3 flavonoids
- 1 a simple phenol derivative (new for the genus)
- 1 cinamic acid derivative
- 1 phenylpropanoid ester heteroside
The structure determination of the isolated compound was carried out by physical methods such as UV-visible spectrophotometry, the high resolution mass spectrometry with electrospray (HRESIMS), the nuclear magnetic resonance spectroscopy (NMR 1H, NMR 13C, 1H-1H COSY, HSQC, HMBC, NOESY, ROESY).
In continuation of previous works on the species Stachys ocymastrum (L.) Briq. Four flavonoids have been isolated, we’ve identified two other flavonoids from the n-butanolic extract by the use of HPLC-UV analaysis in the presence of the authentic samples.
GC and GC / MS analysis of the essential oil of Salvia officinalis L. led to the identification of 35 compounds with 3 as the major components: the α-thujone (24.52%), camphor (16.86%) and 1,8-cineole (15.92%).
The Butanolic extract of Stachys ocymastrum (L.) Briq. exhibited a good antioxidant activity by the use of the DPPH method.
The essential oil of Salvia officinalis L. especially inhibited the growth of bacterial strains namely Escherichia coli ATCC 25922, Escherichia coli (HS) and Streptococcus homeolytic and showed good antioxidant activity by β-carotene bleaching methode.Diplôme : Doctorat en sciences En ligne : ../theses/chimie/ALEK9356.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9478 تحدید مركبات الأیض الثانوي وَ الدراسة البیولوجیة لنباتات عطریة من جنسي Salvia وَ Stachys [texte imprimé] / هشام لكحل, Auteur ; أحمد كعبوش, Directeur de thèse . - 2014 . - 245 ورقة. ; 30 سم.
مذكرة دكتوراه العلوم
2نسخ موجودة في المكتبة المركزية
Langues : Arabe (ara)
Catégories : Arabe
الكيمياء و العلوم ذات العلاقةTags : triterpènes saponoside phenylpropanoide flavonoides activité antioxydante antibacterienne Stachys Salvia Lamiaceae triterpenes saponin flavonoids phenylpropanoid antioxidant activity antibacterial تربین ثلاثي صابونین فلافونویدات إسترفینیل بروبانوید فعالیة مضادة للأكسدة فعالیة مضادة للبكتیریا Index. décimale : 540 الكيمياء و العلوم ذات العلاقة Résumé : In this work, we conducted the phytochemical study and evaluated the antibacterial and antioxidant activities of plants grown in the Eastern Algerian belonging to the Lamiaceae family : Salvia argentea var. aurasiaca (Pomel) Batt. et Trab., Stachys ocymastrum (L.) Briq. et Salvia officinalis L..
The phytochemical study of the species Salvia argentea var. aurasiaca (Pomel) Batt. et Trab resulted in the isolation of 19 compounds of which 12 were described for the first time in the plant kingdom, The isolated compounds belong to six classes of secondary metabolites:
- 12 triterpenes
- 1 saponin
- 3 flavonoids
- 1 a simple phenol derivative (new for the genus)
- 1 cinamic acid derivative
- 1 phenylpropanoid ester heteroside
The structure determination of the isolated compound was carried out by physical methods such as UV-visible spectrophotometry, the high resolution mass spectrometry with electrospray (HRESIMS), the nuclear magnetic resonance spectroscopy (NMR 1H, NMR 13C, 1H-1H COSY, HSQC, HMBC, NOESY, ROESY).
In continuation of previous works on the species Stachys ocymastrum (L.) Briq. Four flavonoids have been isolated, we’ve identified two other flavonoids from the n-butanolic extract by the use of HPLC-UV analaysis in the presence of the authentic samples.
GC and GC / MS analysis of the essential oil of Salvia officinalis L. led to the identification of 35 compounds with 3 as the major components: the α-thujone (24.52%), camphor (16.86%) and 1,8-cineole (15.92%).
The Butanolic extract of Stachys ocymastrum (L.) Briq. exhibited a good antioxidant activity by the use of the DPPH method.
The essential oil of Salvia officinalis L. especially inhibited the growth of bacterial strains namely Escherichia coli ATCC 25922, Escherichia coli (HS) and Streptococcus homeolytic and showed good antioxidant activity by β-carotene bleaching methode.Diplôme : Doctorat en sciences En ligne : ../theses/chimie/ALEK9356.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9478 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité لكح/3956 لكح/3956 Thèse Bibliothèque principale Thèses Disponible Documents numériques
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Titre : Étude phytochimique et biologique de trois espèces Algériennes : Calendula stellata Cav. (Asteraceae), Scabiosa stellata L. (Caprifoliaceae) et Salvia barrelieri Etl. (Lamiaceae). Type de document : texte imprimé Auteurs : Meryem Lehbili, Auteur ; Ahmed Kabouche, Directeur de thèse ; Abdulmagid Alabdul Magid, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 375 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Calendula stellata Scabiosa stellata Salvia barrelieri saponosides iridoïdes triterpenoïdes flavonoïdes activité antioxydante activité antimicrobienne cytotoxicité saponosids iridoids triterpenes flavonoids antioxidant activity antimicrobial activity cytotoxicity الفعالیة البیولوجیة مضادة للأكسدة مضادة للبكتیریا سمیة خلویة Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical study of three species: Calendula stellata, Scabiosa stellata and Salvia barrelieri led to the isolation of 55 compounds, from which 16 were new, by the use of different chromatographic methods of separation and purification on solid or liquid support.
The isolated compounds were distributed through 27 saponosids, 7 triterpenes, 11 flavonoids, 7 iridoids and 2 chlorogenic acid derivatives. Two mono-iridoids and two flavonoids showed an important antiradical activity (IC50:7-16 µM). Fourteen compounds exhibited a good antibacterial activity (MIC: 15- 125 µg/mL). The new compounds showed a low to moderate cytotoxic activity against all tested cell lines, except for the monodesmoside, obtained from Scabiosa stellata (Scabiostellatoside F) which exhibited a good antiproliferative effect against HT1080 (IC50 = 12 µM).
Diplôme : Doctorat En ligne : ../theses/chimie/LEH7306.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10921 Étude phytochimique et biologique de trois espèces Algériennes : Calendula stellata Cav. (Asteraceae), Scabiosa stellata L. (Caprifoliaceae) et Salvia barrelieri Etl. (Lamiaceae). [texte imprimé] / Meryem Lehbili, Auteur ; Ahmed Kabouche, Directeur de thèse ; Abdulmagid Alabdul Magid, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 375 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Calendula stellata Scabiosa stellata Salvia barrelieri saponosides iridoïdes triterpenoïdes flavonoïdes activité antioxydante activité antimicrobienne cytotoxicité saponosids iridoids triterpenes flavonoids antioxidant activity antimicrobial activity cytotoxicity الفعالیة البیولوجیة مضادة للأكسدة مضادة للبكتیریا سمیة خلویة Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical study of three species: Calendula stellata, Scabiosa stellata and Salvia barrelieri led to the isolation of 55 compounds, from which 16 were new, by the use of different chromatographic methods of separation and purification on solid or liquid support.
The isolated compounds were distributed through 27 saponosids, 7 triterpenes, 11 flavonoids, 7 iridoids and 2 chlorogenic acid derivatives. Two mono-iridoids and two flavonoids showed an important antiradical activity (IC50:7-16 µM). Fourteen compounds exhibited a good antibacterial activity (MIC: 15- 125 µg/mL). The new compounds showed a low to moderate cytotoxic activity against all tested cell lines, except for the monodesmoside, obtained from Scabiosa stellata (Scabiostellatoside F) which exhibited a good antiproliferative effect against HT1080 (IC50 = 12 µM).
Diplôme : Doctorat En ligne : ../theses/chimie/LEH7306.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10921 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LEH/7306 LEH/7306 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et pharmacologique des espèces Salvia buchananii Hedge (Lamiaceae), Cistanche phelypaea (L.) Coutinho (Orobanchaceae) et Anarrhinum pedatum Desf. (Scrophulariaceae). Type de document : texte imprimé Auteurs : Khadidja Aya Beladjila, Auteur ; Djemaa Berrehal, Directeur de thèse ; Alessandra Braca, Directeur de thèse Mention d'édition : 22/06/2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 405 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie: Chimie pharmaceutique Lamiaceae Orobanchaceae Scrophulariaceae triterpénes phényléthanoïdes iridoïdes Huile essentielle activité biologique RMN et spectroscopie de masse phenylethanoid iridoids essential oil biological activities NMR and mass spectroscopy شاطات بيولوجية الزيوت الطيارة مطيافية الرنين النووي المغناطيسي مطيافية الكتلة التربينات الاريويدات الفلافونيدات فينيل ايثانويدات anti - angiogenique
LamiaceaeIndex. décimale : 540 Chimie et sciences connexes Résumé :
In this thesis, we carried out a phytochemical study on three plants : Salvia buchananii Hedge, Cistanche phelypeae (L.) Coutinho and Anarrhinum pedatum Desf. 37 compounds were isolated by the use of different chromatographic methods of separation and purification. Amongthem, 17 were new molecules. The isolated compounds were classified into many groups : triterpenes, phenylethanoid, iridoids, flavonoids, phényléthanoids. GC and GC/MS analyses of the essential oil of S. buchananii showed that it was mainly represented by : 1,8-cineole (23.3%) α-pinene (19.8%), camphene (16.5%) and the β-pinene (11.5%). This oil showed a good antioxidant activity by the bleaching of β-carotene. The cytotoxic activity of the triterpenes SBR1 and SBR2 showed an interesting antiproliferative activity with similar potency in all cell lines HeLa. In addition, several compounds (CT1-CT7) showed a promising activity on hMAGL. This is the first report of naturally occurring phenyletanoid as a MAGL inhibitor. Moreover, the new iridoid compound (APD14) the A. pedatum exhibited the most potential antiangiogenic activity.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/chimie/BEL7641.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11475 Etude phytochimique et pharmacologique des espèces Salvia buchananii Hedge (Lamiaceae), Cistanche phelypaea (L.) Coutinho (Orobanchaceae) et Anarrhinum pedatum Desf. (Scrophulariaceae). [texte imprimé] / Khadidja Aya Beladjila, Auteur ; Djemaa Berrehal, Directeur de thèse ; Alessandra Braca, Directeur de thèse . - 22/06/2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 405 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie: Chimie pharmaceutique Lamiaceae Orobanchaceae Scrophulariaceae triterpénes phényléthanoïdes iridoïdes Huile essentielle activité biologique RMN et spectroscopie de masse phenylethanoid iridoids essential oil biological activities NMR and mass spectroscopy شاطات بيولوجية الزيوت الطيارة مطيافية الرنين النووي المغناطيسي مطيافية الكتلة التربينات الاريويدات الفلافونيدات فينيل ايثانويدات anti - angiogenique
LamiaceaeIndex. décimale : 540 Chimie et sciences connexes Résumé :
In this thesis, we carried out a phytochemical study on three plants : Salvia buchananii Hedge, Cistanche phelypeae (L.) Coutinho and Anarrhinum pedatum Desf. 37 compounds were isolated by the use of different chromatographic methods of separation and purification. Amongthem, 17 were new molecules. The isolated compounds were classified into many groups : triterpenes, phenylethanoid, iridoids, flavonoids, phényléthanoids. GC and GC/MS analyses of the essential oil of S. buchananii showed that it was mainly represented by : 1,8-cineole (23.3%) α-pinene (19.8%), camphene (16.5%) and the β-pinene (11.5%). This oil showed a good antioxidant activity by the bleaching of β-carotene. The cytotoxic activity of the triterpenes SBR1 and SBR2 showed an interesting antiproliferative activity with similar potency in all cell lines HeLa. In addition, several compounds (CT1-CT7) showed a promising activity on hMAGL. This is the first report of naturally occurring phenyletanoid as a MAGL inhibitor. Moreover, the new iridoid compound (APD14) the A. pedatum exhibited the most potential antiangiogenic activity.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/chimie/BEL7641.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11475 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/7641 BEL/7641 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et évaluation des activités biologiques des espèces : Mentha aquatica, Stachys guyoniana et Thymus dreatensis (LAMIACEAE) Type de document : texte imprimé Auteurs : Maria Ferhat, Auteur ; Zahia Kabouche, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 114 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Lamiaceae Mentha aquatica Stachys guyoniana Thymus dreatensis Triterpènes Huile essentielle Activités biologiques RMN 1D et 2D Triterpenoids Essential oil biological activities NMR 1D and 2D " Lamiaceae aquatica Mentha dreatensis Thymys guyonina Stachys
تقنية الرنين النووي المغناطيسي تربينات نشاطات بيولوجية زيوت طيارة"Index. décimale : 540 Chimie et sciences connexes Résumé : This present work concerns the phytochemical and biological study of three Algerian plants belonging to the Lamiaceae family : Mentha aquatica L., Stachys guyoniana de Noé and Thymus dreatensis Batt. Chromatographic separations and purifications of extracts from the aerial parts and roots of Mentha aquatica led to the isolation and identification of 1 6 compounds, including one new triterpene and 5 triterpene acids which are new for the genus.
The structures of isolated compounds were particularly established by the use of NMR 1D and 2D (1H, 13C, COSY H-H, HMQC and HMBC), high resolution mass spectrometry (ESI-MS and HR-ESI-MS) and by comparison with literature data.
GC and GC/MS analyses of T. dreatensis essential oil showed that it was mainly represented by thymol (28.1%) followed by γ-terpinene (18.7%), thymyl methyl ether (10.9%) and linalool (5.0%). This oil showed a good antioxidant activity by the bleaching of β-carotene.
5 methods were used to evaluate the antioxidant activity of Mentha aquatica and Stachys guyoniana. The latter showed a good activity with 4 methods: β-carotene, DPPH, ABTS and metal chelating whereas Mentha aquatica exhibited a moderate activity with CUPRAC and ABTS tests. Compared with the other tested extracts, the n-butanol extract of
Stachys guyoniana showed the highest anticholinesterase activity.
The two plants showed an antibacterial potential against some bacterial strains, by the use of agar diffusion method and MIC's determination.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/FER6964.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10362 Etude phytochimique et évaluation des activités biologiques des espèces : Mentha aquatica, Stachys guyoniana et Thymus dreatensis (LAMIACEAE) [texte imprimé] / Maria Ferhat, Auteur ; Zahia Kabouche, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 114 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Lamiaceae Mentha aquatica Stachys guyoniana Thymus dreatensis Triterpènes Huile essentielle Activités biologiques RMN 1D et 2D Triterpenoids Essential oil biological activities NMR 1D and 2D " Lamiaceae aquatica Mentha dreatensis Thymys guyonina Stachys
تقنية الرنين النووي المغناطيسي تربينات نشاطات بيولوجية زيوت طيارة"Index. décimale : 540 Chimie et sciences connexes Résumé : This present work concerns the phytochemical and biological study of three Algerian plants belonging to the Lamiaceae family : Mentha aquatica L., Stachys guyoniana de Noé and Thymus dreatensis Batt. Chromatographic separations and purifications of extracts from the aerial parts and roots of Mentha aquatica led to the isolation and identification of 1 6 compounds, including one new triterpene and 5 triterpene acids which are new for the genus.
The structures of isolated compounds were particularly established by the use of NMR 1D and 2D (1H, 13C, COSY H-H, HMQC and HMBC), high resolution mass spectrometry (ESI-MS and HR-ESI-MS) and by comparison with literature data.
GC and GC/MS analyses of T. dreatensis essential oil showed that it was mainly represented by thymol (28.1%) followed by γ-terpinene (18.7%), thymyl methyl ether (10.9%) and linalool (5.0%). This oil showed a good antioxidant activity by the bleaching of β-carotene.
5 methods were used to evaluate the antioxidant activity of Mentha aquatica and Stachys guyoniana. The latter showed a good activity with 4 methods: β-carotene, DPPH, ABTS and metal chelating whereas Mentha aquatica exhibited a moderate activity with CUPRAC and ABTS tests. Compared with the other tested extracts, the n-butanol extract of
Stachys guyoniana showed the highest anticholinesterase activity.
The two plants showed an antibacterial potential against some bacterial strains, by the use of agar diffusion method and MIC's determination.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/FER6964.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10362 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité FER/6964 FER/6964 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude phytochimique et évaluation biologique d’une plante endémique du genre Genista (Fabaceae) : Genista numidica Spach. Type de document : texte imprimé Auteurs : Feryal Benayache, Auteur ; Samir Benayache, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 194 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie organique : phytochimie Fabacées Genista numidica lignanes triterpènes activité antioxydante activité cytotoxique RMN 1D et 2D Fabaceae lignans Triterpenoid antioxidant activity Cytotoxic activity NMR 1D and 2D تربينات النشاط المضاد الاكسدة النشاط السام للخلايا الرنين النووي المغناطيسي Index. décimale : 540 Chimie et sciences connexes Résumé : The present work concerns the phytochemical and biological study of an endemic species belonging to the Fabaceae family: Genista numidica Spach which has been collected from the region of Constantine. Our work on the extraction of the aerial parts of G. numidica followed by the chromatographic separation permitted the isolation of 14 secondary metabolites from the chloroformic and ethyl acetate extracts. The structures of 13 were identified and fully described, including a new triterpene. The molecular structures of isolated compounds were established mainly by the use of NMR 1D and 2D (1H, 13C, TOCSY-1D, COSY, HSQC and HMBC), high resolution mass spectrometry (HRESI-MS) and by comparison with the literature Data. The antioxidant activity of CHCl3, EtOAc and n-BuOH extracts of G.numidica has been investigated by DPPH test, the results showed that the ethyl acetate extract has a good scavenging effect in vitro against the DPPH radical (IC50 = 40.43 ±3.09 µg / ml). The antiproliferative activity of isolates ( lignans and saponins) was investigated on Jurkat, HeLa and MCF-7 cell lines. The most active component, the triterpene, 3-O- β-D-glucopyranosyl-olean-12-en-3β,27,28,29-tetraol, showed activity in all cell lines, an IC50 value of 37 ± 2.3 µM (Jurkat), 35 ± 1.7 µM (HeLa) and 50 ± 3.6 µM (MCF-7) respectively. The mechanism underlying its antiproliferative effect was investigated in HeLa cells. The results showed that this compound affect the cell cycle progression by inducing a robust G2/M bloc in HeLa cells. Note de contenu : Annexe. Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BEN7494.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11326 Etude phytochimique et évaluation biologique d’une plante endémique du genre Genista (Fabaceae) : Genista numidica Spach. [texte imprimé] / Feryal Benayache, Auteur ; Samir Benayache, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 194 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie organique : phytochimie Fabacées Genista numidica lignanes triterpènes activité antioxydante activité cytotoxique RMN 1D et 2D Fabaceae lignans Triterpenoid antioxidant activity Cytotoxic activity NMR 1D and 2D تربينات النشاط المضاد الاكسدة النشاط السام للخلايا الرنين النووي المغناطيسي Index. décimale : 540 Chimie et sciences connexes Résumé : The present work concerns the phytochemical and biological study of an endemic species belonging to the Fabaceae family: Genista numidica Spach which has been collected from the region of Constantine. Our work on the extraction of the aerial parts of G. numidica followed by the chromatographic separation permitted the isolation of 14 secondary metabolites from the chloroformic and ethyl acetate extracts. The structures of 13 were identified and fully described, including a new triterpene. The molecular structures of isolated compounds were established mainly by the use of NMR 1D and 2D (1H, 13C, TOCSY-1D, COSY, HSQC and HMBC), high resolution mass spectrometry (HRESI-MS) and by comparison with the literature Data. The antioxidant activity of CHCl3, EtOAc and n-BuOH extracts of G.numidica has been investigated by DPPH test, the results showed that the ethyl acetate extract has a good scavenging effect in vitro against the DPPH radical (IC50 = 40.43 ±3.09 µg / ml). The antiproliferative activity of isolates ( lignans and saponins) was investigated on Jurkat, HeLa and MCF-7 cell lines. The most active component, the triterpene, 3-O- β-D-glucopyranosyl-olean-12-en-3β,27,28,29-tetraol, showed activity in all cell lines, an IC50 value of 37 ± 2.3 µM (Jurkat), 35 ± 1.7 µM (HeLa) and 50 ± 3.6 µM (MCF-7) respectively. The mechanism underlying its antiproliferative effect was investigated in HeLa cells. The results showed that this compound affect the cell cycle progression by inducing a robust G2/M bloc in HeLa cells. Note de contenu : Annexe. Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BEN7494.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11326 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/7494 BEN/7494 Thèse Bibliothèque principale Thèses Disponible
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