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Partager le résultat de cette recherche Interroger des sources externesL’effet protecteur de l’extrait butanolique de la plante Centaurea maroccana contre la neurotoxicité induite par le cisplatine chez les souris. / Imen Aissous
Titre : L’effet protecteur de l’extrait butanolique de la plante Centaurea maroccana contre la neurotoxicité induite par le cisplatine chez les souris. Type de document : texte imprimé Auteurs : Imen Aissous, Auteur ; Mouad Benrebai, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Importance : 126 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Physio-toxicologie Cellulaire Cisplatine Centaurea maroccana Neurotoxicité Stress oxydative Activités antioxydantes Composés phénoliques Souris Wistar albinos Cisplatin Neurotoxicity Oxidative Stress Antioxidant activities Phenolic compounds Wistar albino’s mice سيسبلاتين نبات Centaurea maroccana السمية العصبية الإجهاد التأكسدي النشاط المضاد للأكسدة المركبات الفينولية فئران سلالة Wistar albinos Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study aims to investigate the possible protective effects of n-BuOH extract of Centaurea maroccana Ball aerial parts against CP-induced neurotoxicity in male Wistar albino’s mice. The potential neuroprotective properties of BECM were studied in vitro and in in vivo model. The in vitro study revealed that n-butanolic extract of Centaurea maroccana possessed a strong antioxidant activity by using different assays, namely DPPH•, ABTS•+, DMSO alkalin, Reducing power, β-carotene-linoleic acid and CUPRAC. Also it has demonstrated a high anti-proliferative activity against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) by the decreases viability of both cancer cell lines. Furthermore, BECM exhibited neuroprotective properties, by the inhibition of acetylcholinesterase (AChE) level and lipid peroxidation (LPO) depending on the concentration in the brain tissue. On the other hand, the in vivo study revealed that the administration of a single dose of PC (8 mg/kg; bw) induced a marked brain damage manifested by a significant increases of both AChE and LDH activities. Also oxidative stress was evident in CP-group by the elevation in lipid peroxidation level (MDA), and the decrease in total antioxidant capacity (TAC), as well as a marked decrease in activity of catalase (CAT), superoxide dismutase (SOD), and the glutathione metabolizing enzyme ( glutathione peroxidase “GPx”, glutathione S-transferase “GST”) and GSH level in brain mitochondrial and cytosolic fractions. Moreover, the brain oxidative stress was associated with multifocal histological changes in cerebral cortex. While, the pretreatment of mice with BECM (200 mg/kg; bw) for 10 days significantly improved the alterations induced by CP in all the parameters mentioned previously with better recovery in the brain tissue. Interestingly, the BECM achieved neuroprotection comparable to vitamin E (100 mg/kg; bw) in most of the evaluated parameters. Thus, it appears that the n-butanolic extract of Centaurea maroccana provides a protective potency against CP-induced neurotoxicity which seems to be linked to its richness in polyphenols confirmed by LC-MS analysis.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/AIS7809.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11641 L’effet protecteur de l’extrait butanolique de la plante Centaurea maroccana contre la neurotoxicité induite par le cisplatine chez les souris. [texte imprimé] / Imen Aissous, Auteur ; Mouad Benrebai, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, [s.d.] . - 126 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Physio-toxicologie Cellulaire Cisplatine Centaurea maroccana Neurotoxicité Stress oxydative Activités antioxydantes Composés phénoliques Souris Wistar albinos Cisplatin Neurotoxicity Oxidative Stress Antioxidant activities Phenolic compounds Wistar albino’s mice سيسبلاتين نبات Centaurea maroccana السمية العصبية الإجهاد التأكسدي النشاط المضاد للأكسدة المركبات الفينولية فئران سلالة Wistar albinos Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study aims to investigate the possible protective effects of n-BuOH extract of Centaurea maroccana Ball aerial parts against CP-induced neurotoxicity in male Wistar albino’s mice. The potential neuroprotective properties of BECM were studied in vitro and in in vivo model. The in vitro study revealed that n-butanolic extract of Centaurea maroccana possessed a strong antioxidant activity by using different assays, namely DPPH•, ABTS•+, DMSO alkalin, Reducing power, β-carotene-linoleic acid and CUPRAC. Also it has demonstrated a high anti-proliferative activity against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) by the decreases viability of both cancer cell lines. Furthermore, BECM exhibited neuroprotective properties, by the inhibition of acetylcholinesterase (AChE) level and lipid peroxidation (LPO) depending on the concentration in the brain tissue. On the other hand, the in vivo study revealed that the administration of a single dose of PC (8 mg/kg; bw) induced a marked brain damage manifested by a significant increases of both AChE and LDH activities. Also oxidative stress was evident in CP-group by the elevation in lipid peroxidation level (MDA), and the decrease in total antioxidant capacity (TAC), as well as a marked decrease in activity of catalase (CAT), superoxide dismutase (SOD), and the glutathione metabolizing enzyme ( glutathione peroxidase “GPx”, glutathione S-transferase “GST”) and GSH level in brain mitochondrial and cytosolic fractions. Moreover, the brain oxidative stress was associated with multifocal histological changes in cerebral cortex. While, the pretreatment of mice with BECM (200 mg/kg; bw) for 10 days significantly improved the alterations induced by CP in all the parameters mentioned previously with better recovery in the brain tissue. Interestingly, the BECM achieved neuroprotection comparable to vitamin E (100 mg/kg; bw) in most of the evaluated parameters. Thus, it appears that the n-butanolic extract of Centaurea maroccana provides a protective potency against CP-induced neurotoxicity which seems to be linked to its richness in polyphenols confirmed by LC-MS analysis.
Note de contenu :
Annexes.Diplôme : Doctorat En ligne : ../theses/biologie/AIS7809.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11641 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AIS/7809 AIS/7809 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo Type de document : texte imprimé Auteurs : Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 140 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds التوتر التأكسدي الفعل المضاد للأكسدة المستخلص النباتي المركبات الفينولية الأكسدة الفوقية للدهونIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10833 L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo [texte imprimé] / Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 140 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds التوتر التأكسدي الفعل المضاد للأكسدة المستخلص النباتي المركبات الفينولية الأكسدة الفوقية للدهونIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10833 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAH/7238 LAH/7238 Thèse Bibliothèque principale Thèses Disponible
