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Titre : Biodiversité bactérienne du sol rhizosphérique de Capsicum annuum : activités des PGPB et exploitation des endophytes pour l'amélioration de la croissance et le rendement. Type de document : texte imprimé Auteurs : Karima Khenaka, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse Mention d'édition : 25/02/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 192 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie Appliquée et Biotechnologies Microbiennes piment fort rhizosphère rhizobactérie diversité génétique endophyte activités PGPB Chili pepper rhizosphere rhizobacteria genetic diversity PGPB activities الفليفلة الحولية نطاق الجذور البكتريا الجذرية التنوع الوراثي النابوت الداخلي ، أنشطةPGP Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study was conducted to estimate the bacterial biodiversity of rhizospheric soil and to characterize the activities of plant growth-promotimg bacteria (PGPB) associated to the roots of chili pepper (Capsicum annuum). Rhizospheric and non-rhizospheric soil samples and the roots of three industrial chili pepper genotypes were collected from an agricultural land in the Wilaya of Guelma. Compared to bulk soil, the rhizosphere of the three genotypes has different soil texture; low pH and low carbonate content. Bacterial growth is most abundant around the roots of the three genotypes with different rhizosphere/soil ratios. Analysis of genetic diversity through T-RFLP showed that bacterial diversity increased significantly only in the rhizosphere of Biskra genotype. 515 bacterial isolates were collected, 301 isolates from the three rhizospheres and 214 isolates from bulk soil. The analysis of PGPB specific activities revealed that the production of indole compounds was stimulated in the rhizosphere of the three cultivars; however, the antiphytopathogenic activity was stimulated only by one genotype. The bacterial isolation from the roots of the three cultivars yielded 83 endophytes. Amplification by BOX-PCR revealed high genetic diversity of the isolates. Endophytes have shown high plant growth promoting abilities in vitro. All isolates produce auxins via tryptophan-dependent synthetic pathways. GC-MS analysis shows that indole acetic acid is predominantly produced by IPyA pathway. Endophytes have important ability to produce siderophores but with a less chelating capacity than the rhizospheric microbiota. The use of UHPLC-MS/MS Q Exactive detect 23 different iron chelators in 21 isolates. The production of long-chain acyl-homoserine lactones (AHL) is detected in seven isolates. However, inhibition of AHL auto-induction is observed in 22 endophytes, among of which 10 and 15 isolates inhibited auto-induction in C. violaceum CV026 and A. tumefaciens NT1 respectively. Antifungal activity analysis revealed that 25 isolates could inhibit F. oxysporum growth. In addition, 79 % of endophytes have the capacity to solubilize inorganic phosphate. The isolates KEK36, KEK1, FEK22, FEO15 and BEK8 were selected for in vivo study effect. 16S rDNA amplification classified the isolate FEO15 as a member of the Streptomyces genus with high similarity to the species S. caeruleatus. The isolates FEK22, BEK8, KEK36 and KEK1, which belong to the genus Pseudomonas, are phylogenetically similar to the species: P.
moorei, P. japonica, P. granadensis and P. brassicacearum respectively. In vivo, these five strains increased significantly the growth parameters for the three genotypes. FEO15 and FEK22 isolates were selected to study the impact on yield after five months in culture, with separate and combined inoculations. Our results show that the consortium is more efficient than separate inoculation. Karam genotype shows 199 % and 377 % improvement in fruit dry weight after the treatment by FEO15 isolate and the consortium respectively. In addition, an increase of 58 % and 138 % is reported after Al-Fares genotype inoculation with FEO15 isolate and the mixture respectively. As a conclusion from this study, the consortium of FEO15 and FEK22 isolates showed promising results concerning their use as biostimulaters. However, further studies are required to complete the formulation and field application steps.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KHE7715.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11549 Biodiversité bactérienne du sol rhizosphérique de Capsicum annuum : activités des PGPB et exploitation des endophytes pour l'amélioration de la croissance et le rendement. [texte imprimé] / Karima Khenaka, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse . - 25/02/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 192 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie Appliquée et Biotechnologies Microbiennes piment fort rhizosphère rhizobactérie diversité génétique endophyte activités PGPB Chili pepper rhizosphere rhizobacteria genetic diversity PGPB activities الفليفلة الحولية نطاق الجذور البكتريا الجذرية التنوع الوراثي النابوت الداخلي ، أنشطةPGP Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study was conducted to estimate the bacterial biodiversity of rhizospheric soil and to characterize the activities of plant growth-promotimg bacteria (PGPB) associated to the roots of chili pepper (Capsicum annuum). Rhizospheric and non-rhizospheric soil samples and the roots of three industrial chili pepper genotypes were collected from an agricultural land in the Wilaya of Guelma. Compared to bulk soil, the rhizosphere of the three genotypes has different soil texture; low pH and low carbonate content. Bacterial growth is most abundant around the roots of the three genotypes with different rhizosphere/soil ratios. Analysis of genetic diversity through T-RFLP showed that bacterial diversity increased significantly only in the rhizosphere of Biskra genotype. 515 bacterial isolates were collected, 301 isolates from the three rhizospheres and 214 isolates from bulk soil. The analysis of PGPB specific activities revealed that the production of indole compounds was stimulated in the rhizosphere of the three cultivars; however, the antiphytopathogenic activity was stimulated only by one genotype. The bacterial isolation from the roots of the three cultivars yielded 83 endophytes. Amplification by BOX-PCR revealed high genetic diversity of the isolates. Endophytes have shown high plant growth promoting abilities in vitro. All isolates produce auxins via tryptophan-dependent synthetic pathways. GC-MS analysis shows that indole acetic acid is predominantly produced by IPyA pathway. Endophytes have important ability to produce siderophores but with a less chelating capacity than the rhizospheric microbiota. The use of UHPLC-MS/MS Q Exactive detect 23 different iron chelators in 21 isolates. The production of long-chain acyl-homoserine lactones (AHL) is detected in seven isolates. However, inhibition of AHL auto-induction is observed in 22 endophytes, among of which 10 and 15 isolates inhibited auto-induction in C. violaceum CV026 and A. tumefaciens NT1 respectively. Antifungal activity analysis revealed that 25 isolates could inhibit F. oxysporum growth. In addition, 79 % of endophytes have the capacity to solubilize inorganic phosphate. The isolates KEK36, KEK1, FEK22, FEO15 and BEK8 were selected for in vivo study effect. 16S rDNA amplification classified the isolate FEO15 as a member of the Streptomyces genus with high similarity to the species S. caeruleatus. The isolates FEK22, BEK8, KEK36 and KEK1, which belong to the genus Pseudomonas, are phylogenetically similar to the species: P.
moorei, P. japonica, P. granadensis and P. brassicacearum respectively. In vivo, these five strains increased significantly the growth parameters for the three genotypes. FEO15 and FEK22 isolates were selected to study the impact on yield after five months in culture, with separate and combined inoculations. Our results show that the consortium is more efficient than separate inoculation. Karam genotype shows 199 % and 377 % improvement in fruit dry weight after the treatment by FEO15 isolate and the consortium respectively. In addition, an increase of 58 % and 138 % is reported after Al-Fares genotype inoculation with FEO15 isolate and the mixture respectively. As a conclusion from this study, the consortium of FEO15 and FEK22 isolates showed promising results concerning their use as biostimulaters. However, further studies are required to complete the formulation and field application steps.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KHE7715.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11549 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KHE/7715 KHE/7715 Thèse Bibliothèque principale Thèses Disponible Extraction, purification et caractérisation des lectines produites par des souches pures d’actinomycètes isolés à partir de la rhizosphère de Lactuca sativa, Vicia fabae, Prunus domestica et P inus halepensis. / Khaled Boulahrouf
Titre : Extraction, purification et caractérisation des lectines produites par des souches pures d’actinomycètes isolés à partir de la rhizosphère de Lactuca sativa, Vicia fabae, Prunus domestica et P inus halepensis. : Tests biologiques des lectines caractérisées. Type de document : texte imprimé Auteurs : Khaled Boulahrouf, Auteur ; Youcef Necib, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 100 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie Biotechnologies microbiennes Génomes et Environnement Actinobacteria actinomycètes aéorobies rhizosphère lectines
glycoconjugués fermentation purification des proteines immunostimulation actinomycetes aéorobies rhizosphere lectins glycoconjugates purification of proteins immunostimulatory ليكتين التخمير الكروموتوغرافي تنشيط المناعةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Lectins are present in all living organisms and are used as tools in research and in the
biomedical sector. Currently, the microbial lectins are attracting more and more of interest
because of their diverse biological activities.
Some strains of actinomycetes Rhizospheric promoters of plant growth and antagonistic fungi and pathogens of these plants were chosen to highlight the production of lectins to purify characterize and study their biological activities. The six strains studied were identified to genera Streptomyces and Nocardiopsis. The strains were cultured on two liquids (Bennet and ISP2). The adhesive strength of the extracellular and intracellular extracts of the culture media is evidence placed in the presence of seven types of erythrocytes.
Both extracts of six strains, in any case of the medium, have no agglutinate human erythrocytes.
Strongest agglutination was obtained with the presence of intracellular extracts and rat erythrocytes of rabbits. The ISP2 environment better promotes the production of lectins. Intracellular fractions strongly caking rat erythrocytes were retained. That of Pru16
strain for purification and characterization and that of the Vic8 strain to study the
immunomodulatory effect.
Lectins, the intracellular fraction of strain Pru16 underwent a first primary purification
with ammonium sulphate and gel filtration G75. Differential precipitation showed that the
first three fractions exhibit HA activity. After filtration, two peaks were observed (peaks A
and B). Peak B shows no HA activity. However, the specific activity of the crude extract was
8.37 HU / mg, and it increased to 14.95 UH / mg after ammonium sulfate precipitation, and
20 UH / mg (picA) .after G75 gel filtration. The HA activity of this fraction remains at
alkaline pH and high concentrations of EDTA.
After column chromatography mucin sepharose 4B (second purification), two peaks
are observed. The proteic fraction 33 peak 2 has a high specific HA activity about twelve
times greater than that observed for the crude extract (8.37 111.49 against UH / mg), however
the first peak shows no HA activity. The affinity test conducted with the proteic fraction 33 of Peak 2 showed that the Pru16 strain binds specifically to protein. The SDS-PAGE analysis
showed that the hemagglutinin contained in the portion 33 of the P2 peak is located in the
band 16kDa The identification of proteins 16kDa band, after digestion and elution of peptides by LC, was top by tandem mass spectrometry proteomics comparing with the database related
strains.
The identified analysis of 6 different proteins similar, one of them has a high score,
with a molecular weight similar to that for protein band. This is the lysozyme C who did him
no HA activity. Our protein of 16kDa presents antimicrobial activity.
The enhancement of the phagocytic index of the corrected phagocytic index, and decreased half-life time in circulation of carbon particles in the blood indicate that the Vic8 strain of the crude extract possess one or more factors able to significantly boost the phagocytic activity of macrophages and reticuloendothelial system in mice. This effect is comparable to that exerted by the pure lectins.Diplôme : Doctorat En ligne : ../theses/biologie/BOU6904.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10236 Extraction, purification et caractérisation des lectines produites par des souches pures d’actinomycètes isolés à partir de la rhizosphère de Lactuca sativa, Vicia fabae, Prunus domestica et P inus halepensis. : Tests biologiques des lectines caractérisées. [texte imprimé] / Khaled Boulahrouf, Auteur ; Youcef Necib, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 100 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie Biotechnologies microbiennes Génomes et Environnement Actinobacteria actinomycètes aéorobies rhizosphère lectines
glycoconjugués fermentation purification des proteines immunostimulation actinomycetes aéorobies rhizosphere lectins glycoconjugates purification of proteins immunostimulatory ليكتين التخمير الكروموتوغرافي تنشيط المناعةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Lectins are present in all living organisms and are used as tools in research and in the
biomedical sector. Currently, the microbial lectins are attracting more and more of interest
because of their diverse biological activities.
Some strains of actinomycetes Rhizospheric promoters of plant growth and antagonistic fungi and pathogens of these plants were chosen to highlight the production of lectins to purify characterize and study their biological activities. The six strains studied were identified to genera Streptomyces and Nocardiopsis. The strains were cultured on two liquids (Bennet and ISP2). The adhesive strength of the extracellular and intracellular extracts of the culture media is evidence placed in the presence of seven types of erythrocytes.
Both extracts of six strains, in any case of the medium, have no agglutinate human erythrocytes.
Strongest agglutination was obtained with the presence of intracellular extracts and rat erythrocytes of rabbits. The ISP2 environment better promotes the production of lectins. Intracellular fractions strongly caking rat erythrocytes were retained. That of Pru16
strain for purification and characterization and that of the Vic8 strain to study the
immunomodulatory effect.
Lectins, the intracellular fraction of strain Pru16 underwent a first primary purification
with ammonium sulphate and gel filtration G75. Differential precipitation showed that the
first three fractions exhibit HA activity. After filtration, two peaks were observed (peaks A
and B). Peak B shows no HA activity. However, the specific activity of the crude extract was
8.37 HU / mg, and it increased to 14.95 UH / mg after ammonium sulfate precipitation, and
20 UH / mg (picA) .after G75 gel filtration. The HA activity of this fraction remains at
alkaline pH and high concentrations of EDTA.
After column chromatography mucin sepharose 4B (second purification), two peaks
are observed. The proteic fraction 33 peak 2 has a high specific HA activity about twelve
times greater than that observed for the crude extract (8.37 111.49 against UH / mg), however
the first peak shows no HA activity. The affinity test conducted with the proteic fraction 33 of Peak 2 showed that the Pru16 strain binds specifically to protein. The SDS-PAGE analysis
showed that the hemagglutinin contained in the portion 33 of the P2 peak is located in the
band 16kDa The identification of proteins 16kDa band, after digestion and elution of peptides by LC, was top by tandem mass spectrometry proteomics comparing with the database related
strains.
The identified analysis of 6 different proteins similar, one of them has a high score,
with a molecular weight similar to that for protein band. This is the lysozyme C who did him
no HA activity. Our protein of 16kDa presents antimicrobial activity.
The enhancement of the phagocytic index of the corrected phagocytic index, and decreased half-life time in circulation of carbon particles in the blood indicate that the Vic8 strain of the crude extract possess one or more factors able to significantly boost the phagocytic activity of macrophages and reticuloendothelial system in mice. This effect is comparable to that exerted by the pure lectins.Diplôme : Doctorat En ligne : ../theses/biologie/BOU6904.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10236 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6904 BOU/6904 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolement et identification des actinomycètes antagonistes des microorganismes phytopathogènes Type de document : texte imprimé Auteurs : Lamia Aouar, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 221 f. Format : 30 cm. Note générale : Doctorat en sciences
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : champignons phytopathogènes lutte biologique activité antifongique Streptomyces scabies rhizosphère isolement Nocardiopsis Streptomyces gale commune Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AOU6292.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=6276 Isolement et identification des actinomycètes antagonistes des microorganismes phytopathogènes [texte imprimé] / Lamia Aouar, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 221 f. ; 30 cm.
Doctorat en sciences
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : champignons phytopathogènes lutte biologique activité antifongique Streptomyces scabies rhizosphère isolement Nocardiopsis Streptomyces gale commune Index. décimale : 570 Sciences de la vie. Biologie Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AOU6292.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=6276 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AOU/6292 AOU/6292 Thèse Bibliothèque principale Thèses Disponible
