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Titre : Biodiversité bactérienne du sol rhizosphérique de Capsicum annuum : activités des PGPB et exploitation des endophytes pour l'amélioration de la croissance et le rendement. Type de document : texte imprimé Auteurs : Karima Khenaka, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse Mention d'édition : 25/02/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 192 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie Appliquée et Biotechnologies Microbiennes piment fort rhizosphère rhizobactérie diversité génétique endophyte activités PGPB Chili pepper rhizosphere rhizobacteria genetic diversity PGPB activities الفليفلة الحولية نطاق الجذور البكتريا الجذرية التنوع الوراثي النابوت الداخلي ، أنشطةPGP Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study was conducted to estimate the bacterial biodiversity of rhizospheric soil and to characterize the activities of plant growth-promotimg bacteria (PGPB) associated to the roots of chili pepper (Capsicum annuum). Rhizospheric and non-rhizospheric soil samples and the roots of three industrial chili pepper genotypes were collected from an agricultural land in the Wilaya of Guelma. Compared to bulk soil, the rhizosphere of the three genotypes has different soil texture; low pH and low carbonate content. Bacterial growth is most abundant around the roots of the three genotypes with different rhizosphere/soil ratios. Analysis of genetic diversity through T-RFLP showed that bacterial diversity increased significantly only in the rhizosphere of Biskra genotype. 515 bacterial isolates were collected, 301 isolates from the three rhizospheres and 214 isolates from bulk soil. The analysis of PGPB specific activities revealed that the production of indole compounds was stimulated in the rhizosphere of the three cultivars; however, the antiphytopathogenic activity was stimulated only by one genotype. The bacterial isolation from the roots of the three cultivars yielded 83 endophytes. Amplification by BOX-PCR revealed high genetic diversity of the isolates. Endophytes have shown high plant growth promoting abilities in vitro. All isolates produce auxins via tryptophan-dependent synthetic pathways. GC-MS analysis shows that indole acetic acid is predominantly produced by IPyA pathway. Endophytes have important ability to produce siderophores but with a less chelating capacity than the rhizospheric microbiota. The use of UHPLC-MS/MS Q Exactive detect 23 different iron chelators in 21 isolates. The production of long-chain acyl-homoserine lactones (AHL) is detected in seven isolates. However, inhibition of AHL auto-induction is observed in 22 endophytes, among of which 10 and 15 isolates inhibited auto-induction in C. violaceum CV026 and A. tumefaciens NT1 respectively. Antifungal activity analysis revealed that 25 isolates could inhibit F. oxysporum growth. In addition, 79 % of endophytes have the capacity to solubilize inorganic phosphate. The isolates KEK36, KEK1, FEK22, FEO15 and BEK8 were selected for in vivo study effect. 16S rDNA amplification classified the isolate FEO15 as a member of the Streptomyces genus with high similarity to the species S. caeruleatus. The isolates FEK22, BEK8, KEK36 and KEK1, which belong to the genus Pseudomonas, are phylogenetically similar to the species: P.
moorei, P. japonica, P. granadensis and P. brassicacearum respectively. In vivo, these five strains increased significantly the growth parameters for the three genotypes. FEO15 and FEK22 isolates were selected to study the impact on yield after five months in culture, with separate and combined inoculations. Our results show that the consortium is more efficient than separate inoculation. Karam genotype shows 199 % and 377 % improvement in fruit dry weight after the treatment by FEO15 isolate and the consortium respectively. In addition, an increase of 58 % and 138 % is reported after Al-Fares genotype inoculation with FEO15 isolate and the mixture respectively. As a conclusion from this study, the consortium of FEO15 and FEK22 isolates showed promising results concerning their use as biostimulaters. However, further studies are required to complete the formulation and field application steps.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KHE7715.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11549 Biodiversité bactérienne du sol rhizosphérique de Capsicum annuum : activités des PGPB et exploitation des endophytes pour l'amélioration de la croissance et le rendement. [texte imprimé] / Karima Khenaka, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse . - 25/02/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 192 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Microbiologie Appliquée et Biotechnologies Microbiennes piment fort rhizosphère rhizobactérie diversité génétique endophyte activités PGPB Chili pepper rhizosphere rhizobacteria genetic diversity PGPB activities الفليفلة الحولية نطاق الجذور البكتريا الجذرية التنوع الوراثي النابوت الداخلي ، أنشطةPGP Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study was conducted to estimate the bacterial biodiversity of rhizospheric soil and to characterize the activities of plant growth-promotimg bacteria (PGPB) associated to the roots of chili pepper (Capsicum annuum). Rhizospheric and non-rhizospheric soil samples and the roots of three industrial chili pepper genotypes were collected from an agricultural land in the Wilaya of Guelma. Compared to bulk soil, the rhizosphere of the three genotypes has different soil texture; low pH and low carbonate content. Bacterial growth is most abundant around the roots of the three genotypes with different rhizosphere/soil ratios. Analysis of genetic diversity through T-RFLP showed that bacterial diversity increased significantly only in the rhizosphere of Biskra genotype. 515 bacterial isolates were collected, 301 isolates from the three rhizospheres and 214 isolates from bulk soil. The analysis of PGPB specific activities revealed that the production of indole compounds was stimulated in the rhizosphere of the three cultivars; however, the antiphytopathogenic activity was stimulated only by one genotype. The bacterial isolation from the roots of the three cultivars yielded 83 endophytes. Amplification by BOX-PCR revealed high genetic diversity of the isolates. Endophytes have shown high plant growth promoting abilities in vitro. All isolates produce auxins via tryptophan-dependent synthetic pathways. GC-MS analysis shows that indole acetic acid is predominantly produced by IPyA pathway. Endophytes have important ability to produce siderophores but with a less chelating capacity than the rhizospheric microbiota. The use of UHPLC-MS/MS Q Exactive detect 23 different iron chelators in 21 isolates. The production of long-chain acyl-homoserine lactones (AHL) is detected in seven isolates. However, inhibition of AHL auto-induction is observed in 22 endophytes, among of which 10 and 15 isolates inhibited auto-induction in C. violaceum CV026 and A. tumefaciens NT1 respectively. Antifungal activity analysis revealed that 25 isolates could inhibit F. oxysporum growth. In addition, 79 % of endophytes have the capacity to solubilize inorganic phosphate. The isolates KEK36, KEK1, FEK22, FEO15 and BEK8 were selected for in vivo study effect. 16S rDNA amplification classified the isolate FEO15 as a member of the Streptomyces genus with high similarity to the species S. caeruleatus. The isolates FEK22, BEK8, KEK36 and KEK1, which belong to the genus Pseudomonas, are phylogenetically similar to the species: P.
moorei, P. japonica, P. granadensis and P. brassicacearum respectively. In vivo, these five strains increased significantly the growth parameters for the three genotypes. FEO15 and FEK22 isolates were selected to study the impact on yield after five months in culture, with separate and combined inoculations. Our results show that the consortium is more efficient than separate inoculation. Karam genotype shows 199 % and 377 % improvement in fruit dry weight after the treatment by FEO15 isolate and the consortium respectively. In addition, an increase of 58 % and 138 % is reported after Al-Fares genotype inoculation with FEO15 isolate and the mixture respectively. As a conclusion from this study, the consortium of FEO15 and FEK22 isolates showed promising results concerning their use as biostimulaters. However, further studies are required to complete the formulation and field application steps.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/KHE7715.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11549 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KHE/7715 KHE/7715 Thèse Bibliothèque principale Thèses Disponible
Titre : Diversité phénotypique et moléculaire du blé dur cultivé en Algérie : identification et caractérisation des accessions. Type de document : texte imprimé Auteurs : Amina Khennaoui, Auteur ; Nadia Ykhlef, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 169 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : traits agro- morphologiques Triticum durum rendement diversité génétique SSR RAPD agro-morphological traits yield genetic diversity الصفات المورفولوجیة الزراعیة القمح الصلب المردود التنوع الجیني المؤشرات الجزیئیة .(SSR, RAPD) Index. décimale : 570 Sciences de la vie. Biologie Résumé : The genetic improvement of the agronomic traits related to the performance and adaptation to the environments is conditioned by the presence of a genetic and phenotypic variability of the targeted traits in the plant material subjected to the selection. In this work, three trials were conducted on the collection of 26 durum wheat (Triticum durum Desf.) from various origins. The first trial was realized during the campaign of 2011/2012 on the
ITGC site of el khroub station in rainy conditions. This site represents the highest Algerian eastern plains. To assess the extent of phenotypic variability for future use. Various phenological and physiological parameters (The heading stage and maturity date, the temperature of crop canopy, relative water content), agronomics (The number of fertile tillers (NTF), the number of grass tillers (NTG), and (NTF/NTG). And quantitative morphological characteristics (plant height, flag leaf area, the length of beards, the spike length) and qualitative characteristics (spike and beards color and pubescence of glumes) were studied in the genotypes of durum wheat. The results obtained reveal highly significant varietal differences for all measured parameters.
The second trial was conducted during the 2012/2013 campaign on the same site of previous experiments; in order to compare the production and performance of varieties studied under different climatic conditions. A significant effect years and varieties were revealed for the set of parameters, our results show that the end-of-cycle thermal stress induces a decrease of thousand kernel weight. Lack of water causes a decrease in the number of heads per m² and the number of grains per ear of all varieties. The Hoggar and Tassili varieties have good production capacity during the two trials compared to the other varieties. Highly positive significant links have been found between grain yield and the number of grains produced per unit area, and the number of grain per spike. The principal component analysis, described a total variation of 77.36 %, and the hierarchical
classification has divided the genotypes according to their phenotypic variation into three distinct groups. The first one includes old varieties showing adaptability to climatic conditions to the studied area. They are late and large. In opposition to the two groups formed by varieties from (CIMMYT, ICARDA, and Italy) . They are characterized by a small, early maturity at heading, and show higher values for all performance components.
The third trial was conducted in this study to evaluate the genetic diversity of durum wheat varieties using two types of molecular markers (SSR and RAPD). In total, 44 and 30 alleles were generated by 10 SSR primers and 05 RAPD primers respectively. The results obtained by the use of microsatellites, highlights variability within the 26 varieties.
Reveal significant allelic diversity characterized by high polymorphic information content (PIC) values, highest are obtained by primers WMC 307 and BARC 142 with (0.85 and 0.73) respectively. And in agreement with the large number of polymorphic alleles detected in varieties with an average of 11.8 alleles.These results reinforces and justify the choice to use these primers association analysis in our durum wheat varieties. The
classification of varieties according to UPGMA (Unweighted Pair Group Method with Arithmetic averaging) showed a wide genetic diversity, can be used in the choice of parents in the breeding program. Modern wheat varieties have diverged from Algerian and Italian durum wheat into two main distinct clusters. The results estimated by the RAPD
markers also reveal a genetic polymorphism expressed by a PIC (0.31) and Shannon diversity index (0.410). AMOVA (Analysis of Molecular Variance) showed that genetic variation within groups represented 97% (ΦPT = 0.173 p <0.05).
Diplôme : Doctorat En ligne : ../theses/biologie/KHE7229.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10798 Diversité phénotypique et moléculaire du blé dur cultivé en Algérie : identification et caractérisation des accessions. [texte imprimé] / Amina Khennaoui, Auteur ; Nadia Ykhlef, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 169 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : traits agro- morphologiques Triticum durum rendement diversité génétique SSR RAPD agro-morphological traits yield genetic diversity الصفات المورفولوجیة الزراعیة القمح الصلب المردود التنوع الجیني المؤشرات الجزیئیة .(SSR, RAPD) Index. décimale : 570 Sciences de la vie. Biologie Résumé : The genetic improvement of the agronomic traits related to the performance and adaptation to the environments is conditioned by the presence of a genetic and phenotypic variability of the targeted traits in the plant material subjected to the selection. In this work, three trials were conducted on the collection of 26 durum wheat (Triticum durum Desf.) from various origins. The first trial was realized during the campaign of 2011/2012 on the
ITGC site of el khroub station in rainy conditions. This site represents the highest Algerian eastern plains. To assess the extent of phenotypic variability for future use. Various phenological and physiological parameters (The heading stage and maturity date, the temperature of crop canopy, relative water content), agronomics (The number of fertile tillers (NTF), the number of grass tillers (NTG), and (NTF/NTG). And quantitative morphological characteristics (plant height, flag leaf area, the length of beards, the spike length) and qualitative characteristics (spike and beards color and pubescence of glumes) were studied in the genotypes of durum wheat. The results obtained reveal highly significant varietal differences for all measured parameters.
The second trial was conducted during the 2012/2013 campaign on the same site of previous experiments; in order to compare the production and performance of varieties studied under different climatic conditions. A significant effect years and varieties were revealed for the set of parameters, our results show that the end-of-cycle thermal stress induces a decrease of thousand kernel weight. Lack of water causes a decrease in the number of heads per m² and the number of grains per ear of all varieties. The Hoggar and Tassili varieties have good production capacity during the two trials compared to the other varieties. Highly positive significant links have been found between grain yield and the number of grains produced per unit area, and the number of grain per spike. The principal component analysis, described a total variation of 77.36 %, and the hierarchical
classification has divided the genotypes according to their phenotypic variation into three distinct groups. The first one includes old varieties showing adaptability to climatic conditions to the studied area. They are late and large. In opposition to the two groups formed by varieties from (CIMMYT, ICARDA, and Italy) . They are characterized by a small, early maturity at heading, and show higher values for all performance components.
The third trial was conducted in this study to evaluate the genetic diversity of durum wheat varieties using two types of molecular markers (SSR and RAPD). In total, 44 and 30 alleles were generated by 10 SSR primers and 05 RAPD primers respectively. The results obtained by the use of microsatellites, highlights variability within the 26 varieties.
Reveal significant allelic diversity characterized by high polymorphic information content (PIC) values, highest are obtained by primers WMC 307 and BARC 142 with (0.85 and 0.73) respectively. And in agreement with the large number of polymorphic alleles detected in varieties with an average of 11.8 alleles.These results reinforces and justify the choice to use these primers association analysis in our durum wheat varieties. The
classification of varieties according to UPGMA (Unweighted Pair Group Method with Arithmetic averaging) showed a wide genetic diversity, can be used in the choice of parents in the breeding program. Modern wheat varieties have diverged from Algerian and Italian durum wheat into two main distinct clusters. The results estimated by the RAPD
markers also reveal a genetic polymorphism expressed by a PIC (0.31) and Shannon diversity index (0.410). AMOVA (Analysis of Molecular Variance) showed that genetic variation within groups represented 97% (ΦPT = 0.173 p <0.05).
Diplôme : Doctorat En ligne : ../theses/biologie/KHE7229.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10798 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KHE/7229 KHE/7229 Thèse Bibliothèque principale Thèses Disponible
Titre : Les genres Medicago L. et Trifolium L. en Algérie : Diversité morphologique, biochimique et moléculaire Type de document : texte imprimé Auteurs : Imane Medoukali, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 171 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Medicago Trifolium Diversité génétique Algérie Morphologie Propriétés
physiques Isoenzymes Protéines Microsatellites Algeria Genetic diversity Morphology Physical
properties Isozymes Proteins Microsatellite التنوع الوراثي الجزائر النمط الظاهري الخصائص الفيزيائية أشباه ا لنزيمات بروتينات ميكروساتIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The genetic diversity of spontaneous populations of Medicago and Trifolium genera was
analyzed using various morphological, biochemical and molecular approaches. This study is based on a large sampling of populations in a wide range of bioclimatic regions in northern Algeria. Combination of studied markers (biometric characters, isoenzymes, albumins, globulins, seed physical properties and microsatellites) showed an important polymorphism at intra- and interspecific levels in both genera. No significant relationship between the environment of the collection site and morphological features was detected. A considerable number of species-specific bands were detected which can be used for the species identification.
Both species Medicago polymorpha and Trifolium campestre were characterized by greater
intraspecific genetic diversity whatever the marker. Species classification based on different approaches showed some consistencies and contradictions with conventional data based on morphological criteria. Both albumin and globulin fractions were characterized by a significant correlation between them. However, a lack of association was registered with distance matrices generated by the other markers. The detection of morphological, biochemical and molecular markers is of high interest in the perspective of selecting adapted genotypes and for conservation biodiversity.
Diplôme : Doctorat En ligne : ../theses/biologie/MED6916.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10268 Les genres Medicago L. et Trifolium L. en Algérie : Diversité morphologique, biochimique et moléculaire [texte imprimé] / Imane Medoukali, Auteur ; Douadi Khelifi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 171 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Medicago Trifolium Diversité génétique Algérie Morphologie Propriétés
physiques Isoenzymes Protéines Microsatellites Algeria Genetic diversity Morphology Physical
properties Isozymes Proteins Microsatellite التنوع الوراثي الجزائر النمط الظاهري الخصائص الفيزيائية أشباه ا لنزيمات بروتينات ميكروساتIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The genetic diversity of spontaneous populations of Medicago and Trifolium genera was
analyzed using various morphological, biochemical and molecular approaches. This study is based on a large sampling of populations in a wide range of bioclimatic regions in northern Algeria. Combination of studied markers (biometric characters, isoenzymes, albumins, globulins, seed physical properties and microsatellites) showed an important polymorphism at intra- and interspecific levels in both genera. No significant relationship between the environment of the collection site and morphological features was detected. A considerable number of species-specific bands were detected which can be used for the species identification.
Both species Medicago polymorpha and Trifolium campestre were characterized by greater
intraspecific genetic diversity whatever the marker. Species classification based on different approaches showed some consistencies and contradictions with conventional data based on morphological criteria. Both albumin and globulin fractions were characterized by a significant correlation between them. However, a lack of association was registered with distance matrices generated by the other markers. The detection of morphological, biochemical and molecular markers is of high interest in the perspective of selecting adapted genotypes and for conservation biodiversity.
Diplôme : Doctorat En ligne : ../theses/biologie/MED6916.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10268 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MED/6916 MED/6916 Thèse Bibliothèque principale Thèses Disponible Caracterisation et identification de quelques cultivars d’olivier autochtones (Olea europaea l.) apport des marqueurs moleculaires / Messaoud Laib
Titre : Caracterisation et identification de quelques cultivars d’olivier autochtones (Olea europaea l.) apport des marqueurs moleculaires Type de document : texte imprimé Auteurs : Messaoud Laib, Auteur ; Mohamed El Moncef. Bentchikou, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 134 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Olea europaea genetic diversity IOC descriptors polymorphism molecular
markers diversité génétique descripteurs COI polymorphisme marqueurs
moléculaires الزيتون التنوع الوارثي مواصفات ( )COIتعدد الأشكال المؤشرات
الجزيئيةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Our objective was to characterize and identify 15 olive cultivars (Olea europaea L.) grown in
the Skikda region in order to solve taxonomic confusion problems in order to better
understand the local olive-growing heritage.
Morphological characterization was performed using IOC descriptors and genetic
characterization using microsatellites and SNPs.
The results obtained showed that the characters of the endocarp and the fruit are the most
discriminating morphologically. Microsatellite markers allowed complete discrimination of
the different cultivars, whereas the selected SNPs proved ineffective.
This characterization work constitutes a database essential to any program of conservation,
enhancement and genetic improvement of our national olive-growing heritage.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/LAI7058.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10542 Caracterisation et identification de quelques cultivars d’olivier autochtones (Olea europaea l.) apport des marqueurs moleculaires [texte imprimé] / Messaoud Laib, Auteur ; Mohamed El Moncef. Bentchikou, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 134 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Olea europaea genetic diversity IOC descriptors polymorphism molecular
markers diversité génétique descripteurs COI polymorphisme marqueurs
moléculaires الزيتون التنوع الوارثي مواصفات ( )COIتعدد الأشكال المؤشرات
الجزيئيةIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Our objective was to characterize and identify 15 olive cultivars (Olea europaea L.) grown in
the Skikda region in order to solve taxonomic confusion problems in order to better
understand the local olive-growing heritage.
Morphological characterization was performed using IOC descriptors and genetic
characterization using microsatellites and SNPs.
The results obtained showed that the characters of the endocarp and the fruit are the most
discriminating morphologically. Microsatellite markers allowed complete discrimination of
the different cultivars, whereas the selected SNPs proved ineffective.
This characterization work constitutes a database essential to any program of conservation,
enhancement and genetic improvement of our national olive-growing heritage.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/LAI7058.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10542 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAI/7058 LAI/7058 Thèse Bibliothèque principale Thèses Disponible
