Détail de l'auteur
Auteur Nadia Ykhlef |
Documents disponibles écrits par cet auteur (11)
Affiner la recherche Interroger des sources externesAmélioration de l’aptitude à la callogenèse chez la pomme de terre Solanum tuberosm L somatique de la pomme de terre par la sélection de meilleurs équilibres hormonaux. / Chahredine Sadek
Titre : Amélioration de l’aptitude à la callogenèse chez la pomme de terre Solanum tuberosm L somatique de la pomme de terre par la sélection de meilleurs équilibres hormonaux. Type de document : texte imprimé Auteurs : Chahredine Sadek, Auteur ; Nadia Ykhlef, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 142 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : NAA/BAP embryons somatiques 2,4-D/BAP Callus Solanum tuberosum L. somatic embryos الكالس-الأجنة الجسمية Index. décimale : 570 Sciences de la vie. Biologie Résumé : The main objective of this investigation is to study the response of different potato explants Spunta and Kondor to callogenesis and somatic embryogenesis in the presence of combinations with NAA / BAP and 2,4-D / BAP. We opted from the start for the buds and internodes as explants. The color and texture of the calli vary according to the nature of the growth regulators and the presence or absence of light. With the NAA / BAP combination the
Spunta variety develops the best callogenesis in all environments compared to the Kondor variety depending on the concentration of hormones, there is a range of variations of days required for callus initiation, the percentage of explants that have developed calluses, texture, color and degree of its formation. For the combination 2,4-D / BAP the results indicate that after three weeks of culture, the calli are moist, soft and friable in green and whitish-green and white with the NAA / BAP combination, and green, light green and brown color for both
explant with all media with of 2,4-D / BAP combination. Callus growth varies according to culture conditions like light and temperature, callus incubated in the dark give brown colors and a friable to compact structure, while the calluses incubated in light show varying colors (green, light green, white and brown) that are due to the action of light on the photosynthetic pigments. The rate of callogenesis and fresh weight of calli are positively correlated with the concentration of auxin. The histological study of callus obtained from internodal explants after 3 and 60 days shows that cell division is produced in the epidermis only after three days of culture and then spread to the first layer of the cortex. During somatic embryogenesis the calluses of different explants develop with all media (1-2) buds and dense roots. Exceeding three months of incubation the calli are larger and begin to brown and lose the green color after degradation of chlorophyll. A surprising observation in our study which is the formation
of microtubers from the callus of tubercle germs on M1 and M2 medium after 37 to 60 days of incubation. This research has made it possible to highlight the conditions necessary for callogenesis and somatic embryogenesis. In the end it is recommended to try to induce somatic embryogenesis by applying stress (osmotic, with ions, ABA ...), with the addition of organic and mineral nitrogen source for a period exceeding three months.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/SAD7228.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10799 Amélioration de l’aptitude à la callogenèse chez la pomme de terre Solanum tuberosm L somatique de la pomme de terre par la sélection de meilleurs équilibres hormonaux. [texte imprimé] / Chahredine Sadek, Auteur ; Nadia Ykhlef, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 142 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : NAA/BAP embryons somatiques 2,4-D/BAP Callus Solanum tuberosum L. somatic embryos الكالس-الأجنة الجسمية Index. décimale : 570 Sciences de la vie. Biologie Résumé : The main objective of this investigation is to study the response of different potato explants Spunta and Kondor to callogenesis and somatic embryogenesis in the presence of combinations with NAA / BAP and 2,4-D / BAP. We opted from the start for the buds and internodes as explants. The color and texture of the calli vary according to the nature of the growth regulators and the presence or absence of light. With the NAA / BAP combination the
Spunta variety develops the best callogenesis in all environments compared to the Kondor variety depending on the concentration of hormones, there is a range of variations of days required for callus initiation, the percentage of explants that have developed calluses, texture, color and degree of its formation. For the combination 2,4-D / BAP the results indicate that after three weeks of culture, the calli are moist, soft and friable in green and whitish-green and white with the NAA / BAP combination, and green, light green and brown color for both
explant with all media with of 2,4-D / BAP combination. Callus growth varies according to culture conditions like light and temperature, callus incubated in the dark give brown colors and a friable to compact structure, while the calluses incubated in light show varying colors (green, light green, white and brown) that are due to the action of light on the photosynthetic pigments. The rate of callogenesis and fresh weight of calli are positively correlated with the concentration of auxin. The histological study of callus obtained from internodal explants after 3 and 60 days shows that cell division is produced in the epidermis only after three days of culture and then spread to the first layer of the cortex. During somatic embryogenesis the calluses of different explants develop with all media (1-2) buds and dense roots. Exceeding three months of incubation the calli are larger and begin to brown and lose the green color after degradation of chlorophyll. A surprising observation in our study which is the formation
of microtubers from the callus of tubercle germs on M1 and M2 medium after 37 to 60 days of incubation. This research has made it possible to highlight the conditions necessary for callogenesis and somatic embryogenesis. In the end it is recommended to try to induce somatic embryogenesis by applying stress (osmotic, with ions, ABA ...), with the addition of organic and mineral nitrogen source for a period exceeding three months.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/SAD7228.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10799 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité SAD/7228 SAD/7228 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation Botanique et moléculaire du palmier dattier (Phœnix dactylifera L.) de la région de Bou-Sâada. Type de document : texte imprimé Auteurs : Ahlem Guettouchi, Auteur ; Nadia Ykhlef, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 147 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Palmier dattier Bou-Saâda inventaire caractère morphologique ISSR RAPD Bayoud Date palm Bou-Sâada inventory morphological characteristics نخیل التمر بوسعادة حصر الخصائص المرفولوجیة البیوض Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of the genetic diversity of the date palm of the Oasis of Bou-Saâda led us to make
an inventory of palm trees in the four zones Djenane Btom, Djenane Nakhara, Dejnane
Hmaïd, and Djenane khachbat mimoum, Twenty-three varieties without count Dokkar and
the total number of palm trees is 2147. With the study of the morphological characteristics
(length of palm, number of spines ...) it was possible to describe and identify the twenty three
varieties listed, Varieties that exist only at the Oasis of Bou-Saâda as Bou-Saâdiya, Nebgaya
and Zebla.
Molecular identification of the date palm cultivars of the Bou-Saâda palm grove by the two
techniques ISSR (21 primers) and RAPD (27 primers) were used revealed an important
genetic polymorphism inter accessions is explained by the morphological difference between
the varieties. The RAPD and ISSR markers allowed to characterize and distinguish the
different cultivars. Analysis of the RAPD, ISSR data generated a phylogeny similar to that
revealed by RAPD
Concerning the genetic identification of Bayoud-resistant cultivars The use of the two R and S
plasmids as molecular markers will be effective in detecting the Bayoud-resistant cultivars.
Resistant cultivars have been identified from the cultivars tested. It has been found that Baâret
ljmal is resistant, on the other hand, the cultivar Deglet Nour represents the two plasmid forms
R and S. Thus, using this technique, it is possible to establish a list of cultivars of Algerian
date palm Resistant or sensitive to Bayoud
The Oasis of Bou-Saâda still retains an important genetic heritage, which must be protected
and used
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/GUE7139.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10463 Caractérisation Botanique et moléculaire du palmier dattier (Phœnix dactylifera L.) de la région de Bou-Sâada. [texte imprimé] / Ahlem Guettouchi, Auteur ; Nadia Ykhlef, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 147 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Palmier dattier Bou-Saâda inventaire caractère morphologique ISSR RAPD Bayoud Date palm Bou-Sâada inventory morphological characteristics نخیل التمر بوسعادة حصر الخصائص المرفولوجیة البیوض Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of the genetic diversity of the date palm of the Oasis of Bou-Saâda led us to make
an inventory of palm trees in the four zones Djenane Btom, Djenane Nakhara, Dejnane
Hmaïd, and Djenane khachbat mimoum, Twenty-three varieties without count Dokkar and
the total number of palm trees is 2147. With the study of the morphological characteristics
(length of palm, number of spines ...) it was possible to describe and identify the twenty three
varieties listed, Varieties that exist only at the Oasis of Bou-Saâda as Bou-Saâdiya, Nebgaya
and Zebla.
Molecular identification of the date palm cultivars of the Bou-Saâda palm grove by the two
techniques ISSR (21 primers) and RAPD (27 primers) were used revealed an important
genetic polymorphism inter accessions is explained by the morphological difference between
the varieties. The RAPD and ISSR markers allowed to characterize and distinguish the
different cultivars. Analysis of the RAPD, ISSR data generated a phylogeny similar to that
revealed by RAPD
Concerning the genetic identification of Bayoud-resistant cultivars The use of the two R and S
plasmids as molecular markers will be effective in detecting the Bayoud-resistant cultivars.
Resistant cultivars have been identified from the cultivars tested. It has been found that Baâret
ljmal is resistant, on the other hand, the cultivar Deglet Nour represents the two plasmid forms
R and S. Thus, using this technique, it is possible to establish a list of cultivars of Algerian
date palm Resistant or sensitive to Bayoud
The Oasis of Bou-Saâda still retains an important genetic heritage, which must be protected
and used
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/GUE7139.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10463 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité GUE/7139 GUE/7139 Thèse Bibliothèque principale Thèses Disponible Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAM/6868 HAM/6868 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation d’hybrides Aegilops sp./ Blé dur (Triticum durum Desf.) pour l’amélioration génétique de la tolérance à la sécheresse du blé dur : Caractérisation moléculaire par SSR. Type de document : texte imprimé Auteurs : Fethia Zadri ép Boudchicha, Auteur ; Nadia Ykhlef, Directeur de thèse Mention d'édition : 28-12-2020 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2020 Importance : 168 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale: Génomiques et Techniques avancées des végétaux Aegilops blé dur sécheresse croisements interspécifiques amélioration génétique marqueurs SSR durum wheat drought interspecific hybridization genetic breeding SSR markers القمح الصلب الجفاف التحسين الوراثي التهجين مابين الأنواع مؤشرات (SSR ( Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The aim of this work was the obtaining of interspecific hybrids between wheat and wild Aegilops species as the female parent. The Aegilops genus is a potential genes source to genetic breeding for drought tolerance which causes considerable losses for wheat culture, in Algeria. For that, three durum wheat varieties (Oued Zenati, Waha and Hoggar) where crossed with two tetraploid Aegilops species (Aegilops geniculata Roth and Aegilops
triuncialis L.), crosses were realised for 5 years, to figure out parents having most crossability to obtain hybrids. Parents were also characterized for drought tolerance. Invitro mature embryos culture was undertaking, for raison of non hybrids germination in natural conditions. Hybrids were characterized by chromosomes numbering and using eleven microsatellites markers (SSRs) for molecular expression in comparison with their parents.
Results showed a great drought tolerance potential for Aegilops species and adaptation of wheat varieties to climatic conditions governing wheat culture areas. 81 interspecific hybrids were obtained. Aegilops geniculata/Oued Zenati crosses produced the higher hybrids number (54 hybrids as the rate of 5, 23%), followed by Aegilops triuncialis/Oued Zenati (18 as the rate of 3, 88%), crosses involving Aegilops species and variety Hoggar produced the less hybrids number. This direction of hybridization is advantageous, due to hybrid production without embryos rescue. However, lethality and abnormal hybrid development was observed at different stages, any plant hybrid could be generated. For Aegilops geniculata/Oued Zenati, chromosome number was establish to be 2n= 24. From eleven SSR markers, eight were found to be polymorphic, two of which Xbarc174 and Xwms332, had a big band number and better distinguished hybrids. Different patterns of markers expression were observed, even for hybrids having the same parents, where Xwmc25 and Xwms257 allowed characterization of 2BS chromosome fragmentation and elimination in two different Aegilops geniculata/Oued Zenati. These results indicate that maternal cytoplasmic inheritance took place in hybrid formation, and that postzygotic isolation mechanisms explain lethality and abnormal hybrids development, in this direction of hybridization. Searching within Aegilops, of accessions having more cross-ability to ensure hybrid regeneration and succeed such hybridizations.
Note de contenu :
Annexes.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ZAD7759.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11592 Caractérisation d’hybrides Aegilops sp./ Blé dur (Triticum durum Desf.) pour l’amélioration génétique de la tolérance à la sécheresse du blé dur : Caractérisation moléculaire par SSR. [texte imprimé] / Fethia Zadri ép Boudchicha, Auteur ; Nadia Ykhlef, Directeur de thèse . - 28-12-2020 . - جامعة الإخوة منتوري قسنطينة, 2020 . - 168 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie et Ecologie Végétale: Génomiques et Techniques avancées des végétaux Aegilops blé dur sécheresse croisements interspécifiques amélioration génétique marqueurs SSR durum wheat drought interspecific hybridization genetic breeding SSR markers القمح الصلب الجفاف التحسين الوراثي التهجين مابين الأنواع مؤشرات (SSR ( Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The aim of this work was the obtaining of interspecific hybrids between wheat and wild Aegilops species as the female parent. The Aegilops genus is a potential genes source to genetic breeding for drought tolerance which causes considerable losses for wheat culture, in Algeria. For that, three durum wheat varieties (Oued Zenati, Waha and Hoggar) where crossed with two tetraploid Aegilops species (Aegilops geniculata Roth and Aegilops
triuncialis L.), crosses were realised for 5 years, to figure out parents having most crossability to obtain hybrids. Parents were also characterized for drought tolerance. Invitro mature embryos culture was undertaking, for raison of non hybrids germination in natural conditions. Hybrids were characterized by chromosomes numbering and using eleven microsatellites markers (SSRs) for molecular expression in comparison with their parents.
Results showed a great drought tolerance potential for Aegilops species and adaptation of wheat varieties to climatic conditions governing wheat culture areas. 81 interspecific hybrids were obtained. Aegilops geniculata/Oued Zenati crosses produced the higher hybrids number (54 hybrids as the rate of 5, 23%), followed by Aegilops triuncialis/Oued Zenati (18 as the rate of 3, 88%), crosses involving Aegilops species and variety Hoggar produced the less hybrids number. This direction of hybridization is advantageous, due to hybrid production without embryos rescue. However, lethality and abnormal hybrid development was observed at different stages, any plant hybrid could be generated. For Aegilops geniculata/Oued Zenati, chromosome number was establish to be 2n= 24. From eleven SSR markers, eight were found to be polymorphic, two of which Xbarc174 and Xwms332, had a big band number and better distinguished hybrids. Different patterns of markers expression were observed, even for hybrids having the same parents, where Xwmc25 and Xwms257 allowed characterization of 2BS chromosome fragmentation and elimination in two different Aegilops geniculata/Oued Zenati. These results indicate that maternal cytoplasmic inheritance took place in hybrid formation, and that postzygotic isolation mechanisms explain lethality and abnormal hybrids development, in this direction of hybridization. Searching within Aegilops, of accessions having more cross-ability to ensure hybrid regeneration and succeed such hybridizations.
Note de contenu :
Annexes.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ZAD7759.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11592 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité ZAD/7759 ZAD/7759 Thèse Bibliothèque principale Thèses Disponible 
Titre : Caractérisation de la tolérance à la sécheresse chez le blé dur (Triticum durum Desf) : Analyse de la physiologie et de la capacité en production Type de document : texte imprimé Auteurs : Ratiba Bousba, Auteur ; Nadia Ykhlef, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 169 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Triticum durum Desf stress hydrique adaptation Photosynthèse nette WUE R. stomatique TRE osmolytes génétique d'association SSR et PIC water deficit Net Photosynthesis R. stomatal RWC association genetic SSR and PIC القمح القاسي ؛الإجهاد المائي؛المقاوم الثغرية التركيب الضوئي و المؤشرات الجزيئية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Knowledge of the physiological characteristics, biochemical and agronomic processes involved in plant adaptation to abiotic stresses is a milestone in fight against drought. Forty varieties of wheat (Triticum durum) from various sources were used in this study. With three experiments, a semi-controlled test conditions, a field test and a controlled test in growth room, with each experiment, stressful conditions (rain or water deficit) and irrigated. In the first trial several physiological parameterswere studied on six varieties of durum wheat. However, the second trial was conducted in order to compare field production and performance of varieties studied in culture conditions, several measurements were made during development of the plant phenological traits, physiological such as water status of the plant (RWC and RWL) and osmotic potential, morphological parameters and yield and its components. A third experiment was conducted in this study to determine the genetic basis of phenotypic variation. This approach analyzes the statistical association between phenotypic variation and genetic polymorphism using molecular markers like SSR (Simple Sequence Repeat). The results show highly significant genotypic differences for all measured parameters. The recorded observations show that water deficit causes, in all varieties, a progressive decrease of chlorophyll and increased stomatal resistance resulting in a decrease in photosynthetic activity and consequently a decrease in production of dry matter. However, under the same conditions of water stress, introduced varieties have good ability to adaptations ompared to local varieties. Nevertheless the variety (Beliouni) is the variety most productive compared to other local varieties. Positive
correlations were observed betweennet photosynthesis and production ofdry matter. And a significant effect stress and genotype was revealed in the second test, our results show that the water deficit causes a decrease in the relative water content and osmotic potential TRE (Po) of all varieties, which was adjusted by an accumulation of soluble sugarsand proline. This accumulation of osmoticums was very important, very well marked among varieties under stress. Concerning the molecular approach and the genetic association between genotypic and phenotypic variation. The results of this section have shown that phenotypic variation are highly significant, as revealed by a principal component analysis, describing 74% of total variation introduced by two axes ; CP1 and CP2. The hierarchical classification (cluster)show the distribution of genotypes according to their phenotypic variation, based on their origins. For association analysis, the results show that the markers used are significantly associated with the traits studied. The majority of these studied markers were significantly associated with the traits studied , located on chromosome4B. For productivity , three significant SSR markers were detected respectively associated: (WMS149 (p <0.01), WMS30_2) (p <0.05), WMC177_2 ( p <0.05) respectively with 68.94%, 99.46% and 49.74% of phenotypic variation, located on chromosome 4B, 3A and 2A. Also, the hypothesis of association of SSR with heading date (HD) was tested by the GLM (General Linear Model), two SSR markers were found significantly associated with HD they are: (WMS6(p <0.01) located on chromosome 4B, with 96.84% of phenotypic variation, and WMC445(p <0.05) on chromosome 5A with 32.46% of phenotypic variation.
Our results also reveal a significant allelic diversity is characterized by high values of polymorphism information content (PIC), the mean value obtained for all primers was 74%, the highest ones were obtained for WMC177and WMC78markers with (94% and 93%), respectively,
this is in agreement with the large number of alleles detected by these markers, these results reinforces and justify the choice to use these primers association analysis in our durum wheat varieties.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU6394.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9409 Caractérisation de la tolérance à la sécheresse chez le blé dur (Triticum durum Desf) : Analyse de la physiologie et de la capacité en production [texte imprimé] / Ratiba Bousba, Auteur ; Nadia Ykhlef, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 169 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Triticum durum Desf stress hydrique adaptation Photosynthèse nette WUE R. stomatique TRE osmolytes génétique d'association SSR et PIC water deficit Net Photosynthesis R. stomatal RWC association genetic SSR and PIC القمح القاسي ؛الإجهاد المائي؛المقاوم الثغرية التركيب الضوئي و المؤشرات الجزيئية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
Knowledge of the physiological characteristics, biochemical and agronomic processes involved in plant adaptation to abiotic stresses is a milestone in fight against drought. Forty varieties of wheat (Triticum durum) from various sources were used in this study. With three experiments, a semi-controlled test conditions, a field test and a controlled test in growth room, with each experiment, stressful conditions (rain or water deficit) and irrigated. In the first trial several physiological parameterswere studied on six varieties of durum wheat. However, the second trial was conducted in order to compare field production and performance of varieties studied in culture conditions, several measurements were made during development of the plant phenological traits, physiological such as water status of the plant (RWC and RWL) and osmotic potential, morphological parameters and yield and its components. A third experiment was conducted in this study to determine the genetic basis of phenotypic variation. This approach analyzes the statistical association between phenotypic variation and genetic polymorphism using molecular markers like SSR (Simple Sequence Repeat). The results show highly significant genotypic differences for all measured parameters. The recorded observations show that water deficit causes, in all varieties, a progressive decrease of chlorophyll and increased stomatal resistance resulting in a decrease in photosynthetic activity and consequently a decrease in production of dry matter. However, under the same conditions of water stress, introduced varieties have good ability to adaptations ompared to local varieties. Nevertheless the variety (Beliouni) is the variety most productive compared to other local varieties. Positive
correlations were observed betweennet photosynthesis and production ofdry matter. And a significant effect stress and genotype was revealed in the second test, our results show that the water deficit causes a decrease in the relative water content and osmotic potential TRE (Po) of all varieties, which was adjusted by an accumulation of soluble sugarsand proline. This accumulation of osmoticums was very important, very well marked among varieties under stress. Concerning the molecular approach and the genetic association between genotypic and phenotypic variation. The results of this section have shown that phenotypic variation are highly significant, as revealed by a principal component analysis, describing 74% of total variation introduced by two axes ; CP1 and CP2. The hierarchical classification (cluster)show the distribution of genotypes according to their phenotypic variation, based on their origins. For association analysis, the results show that the markers used are significantly associated with the traits studied. The majority of these studied markers were significantly associated with the traits studied , located on chromosome4B. For productivity , three significant SSR markers were detected respectively associated: (WMS149 (p <0.01), WMS30_2) (p <0.05), WMC177_2 ( p <0.05) respectively with 68.94%, 99.46% and 49.74% of phenotypic variation, located on chromosome 4B, 3A and 2A. Also, the hypothesis of association of SSR with heading date (HD) was tested by the GLM (General Linear Model), two SSR markers were found significantly associated with HD they are: (WMS6(p <0.01) located on chromosome 4B, with 96.84% of phenotypic variation, and WMC445(p <0.05) on chromosome 5A with 32.46% of phenotypic variation.
Our results also reveal a significant allelic diversity is characterized by high values of polymorphism information content (PIC), the mean value obtained for all primers was 74%, the highest ones were obtained for WMC177and WMC78markers with (94% and 93%), respectively,
this is in agreement with the large number of alleles detected by these markers, these results reinforces and justify the choice to use these primers association analysis in our durum wheat varieties.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BOU6394.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9409 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6394 BOU/6394 Thèse Bibliothèque principale Thèses Disponible Documents numériques
BOU6394_1.pdfAdobe Acrobat PDF
texte intégraleAdobe Acrobat PDF
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