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Partager le résultat de cette recherche Interroger des sources externesSynthèse, caractérisation et évaluation biologique de nouveaux dérivés poly-hétérocycliques à base d’imidazole / Houssem Boulebd
Titre : Synthèse, caractérisation et évaluation biologique de nouveaux dérivés poly-hétérocycliques à base d’imidazole Type de document : texte imprimé Auteurs : Houssem Boulebd, Auteur ; Ali Belfaitah, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 257 f. Format : 30 cm. Note générale : 2 copies imprimes disponibles Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie organique Imidazole halogénation poly-hétérocycles Maladie d’Alzheimer analogues de la
Tacrine hépatotoxicité ORAC activité biologique إيميدازول هلجنة متعدد الحلقات الغير متجانسة مرض الزهايمر نظائرالتاكرين سمية ORACفعالية بيولوجية
Imidazole halogenation poly-heterocyclic compounds Alzheimer's disease
tacrine analogues hepatotoxicity biological activityIndex. décimale : 540 Chimie et sciences connexes Résumé : Some 2-halo-(1-methyl-1H-imidazole) derivatives were prepared by selective halogenation
reaction (bromination, chlorination and iodination). The addition of -bromoacetophenone to
2-bromo(chloro)-1-methyl-1H-imidazole allowed us to access to corresponding imidazolium
salts in satisfactory yields (47-64%). The reactivity of 2-halogeno-N-phenyl-1-methyl-1Himidazolium bromide with activated methylene compounds of diverse structures has been
studied.
A series of original heterocyclic products composed of a core 1-methyl-1H-imidazole
associated at 2-position to highly functionalized heterocyclic compounds such as 4H-pyran,
chromene, benzo (pyrido) chromenes, 1,4-dihydropyridine, pyridine, quinazoline, pyrrolidines
and other pyrazolines have been prepared.
It will be noted that upon addition of methyl ketone derivatives, 1-methylimidazole-pyridine
hybrid compounds obtained are accompanied by the formation of corresponding 2,6-
dicyanoaniline derivatives in low yields. The adaptation of the operating conditions enabled us
to selectively direct the reaction towards the formation of new 2,6-dicyanoaniline derivatives
in good yields for aliphatic methyl ketone derivatives (62-70%), and in 45 % of yield for
acetophenone. These compounds are also fluorescent.
Some series of hybrid compounds such as (1-methyl-1H-imidazol-2-yl)-4H-pyran, -
pyridine, -1,4-dihydropyridine and -quinazoline have been subjected to an in vitro assessment
of their antimicrobial, and antioxydante (DPPH) activities, and also for their hepatotoxicity
towards liver cells HepG2 in a gradient concentration from 1M to 300M, and a structureactivity relationship (SAR) has been demonstrated.
A "valorization" of (1-methyl-1H-imidazol-2-yl)-4-pyran hybrid derivatives was undertaken
and resulted in the preparation of some original series of highly and diversely functionalized
Tacrine analogues (Tacrine is a drug used in the treatment of Alzheimer's disease) as
pyranotacrines. These novel racemic Tacrine analogues are siginificantly less hepatotoxic than
Tacrine, retaining potent, and selective EeAChE inhibition with IC50 values in the M range,
with a remarkable antioxydant effect.
Yields are good in most cases. All prepared compounds were identified by the usual
spectroscopic methods (IR, 1H NMR and 13C NMR), and for some of them additional analyzes
were carried out (elemental analysis and / or X-ray diffraction).Diplôme : Doctorat En ligne : ../theses/chimie/BOU6833.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10125 Synthèse, caractérisation et évaluation biologique de nouveaux dérivés poly-hétérocycliques à base d’imidazole [texte imprimé] / Houssem Boulebd, Auteur ; Ali Belfaitah, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 257 f. ; 30 cm.
2 copies imprimes disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie organique Imidazole halogénation poly-hétérocycles Maladie d’Alzheimer analogues de la
Tacrine hépatotoxicité ORAC activité biologique إيميدازول هلجنة متعدد الحلقات الغير متجانسة مرض الزهايمر نظائرالتاكرين سمية ORACفعالية بيولوجية
Imidazole halogenation poly-heterocyclic compounds Alzheimer's disease
tacrine analogues hepatotoxicity biological activityIndex. décimale : 540 Chimie et sciences connexes Résumé : Some 2-halo-(1-methyl-1H-imidazole) derivatives were prepared by selective halogenation
reaction (bromination, chlorination and iodination). The addition of -bromoacetophenone to
2-bromo(chloro)-1-methyl-1H-imidazole allowed us to access to corresponding imidazolium
salts in satisfactory yields (47-64%). The reactivity of 2-halogeno-N-phenyl-1-methyl-1Himidazolium bromide with activated methylene compounds of diverse structures has been
studied.
A series of original heterocyclic products composed of a core 1-methyl-1H-imidazole
associated at 2-position to highly functionalized heterocyclic compounds such as 4H-pyran,
chromene, benzo (pyrido) chromenes, 1,4-dihydropyridine, pyridine, quinazoline, pyrrolidines
and other pyrazolines have been prepared.
It will be noted that upon addition of methyl ketone derivatives, 1-methylimidazole-pyridine
hybrid compounds obtained are accompanied by the formation of corresponding 2,6-
dicyanoaniline derivatives in low yields. The adaptation of the operating conditions enabled us
to selectively direct the reaction towards the formation of new 2,6-dicyanoaniline derivatives
in good yields for aliphatic methyl ketone derivatives (62-70%), and in 45 % of yield for
acetophenone. These compounds are also fluorescent.
Some series of hybrid compounds such as (1-methyl-1H-imidazol-2-yl)-4H-pyran, -
pyridine, -1,4-dihydropyridine and -quinazoline have been subjected to an in vitro assessment
of their antimicrobial, and antioxydante (DPPH) activities, and also for their hepatotoxicity
towards liver cells HepG2 in a gradient concentration from 1M to 300M, and a structureactivity relationship (SAR) has been demonstrated.
A "valorization" of (1-methyl-1H-imidazol-2-yl)-4-pyran hybrid derivatives was undertaken
and resulted in the preparation of some original series of highly and diversely functionalized
Tacrine analogues (Tacrine is a drug used in the treatment of Alzheimer's disease) as
pyranotacrines. These novel racemic Tacrine analogues are siginificantly less hepatotoxic than
Tacrine, retaining potent, and selective EeAChE inhibition with IC50 values in the M range,
with a remarkable antioxydant effect.
Yields are good in most cases. All prepared compounds were identified by the usual
spectroscopic methods (IR, 1H NMR and 13C NMR), and for some of them additional analyzes
were carried out (elemental analysis and / or X-ray diffraction).Diplôme : Doctorat En ligne : ../theses/chimie/BOU6833.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10125 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6833 BOU/6833 Thèse Bibliothèque principale Thèses Disponible
Titre : Comportements biologique, physiologique, biochimique et l’activité biologique de quatre variétés d’olivier (Olea europeae L.) dans l’Est Algérien. Type de document : texte imprimé Auteurs : Sara Himour ép Cherrier, Auteur ; Abdelouahab Yahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 209 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Olea europaea L. morphologie physiologie HPLC Oleuropéine activité biologique antioxydante anticoagulante antibactérienne morphology physiology Oleuropein biological activity antioxidant anticoagulant antibacterial اولیااوروبیا Lمورفولوجیا، فیزیولوجیة HPLCأولیوروبین النشاط البیولوجي مضادات الأكسدة مضاد للتخثر مضاد
للبكتیریاIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The present study focuses on the biological, physiological and biochemical behavior of the cultivated olive tree, Olea europaea L., represented by four varieties sampled at the Maazouzi Lakhdar station, Mila: Sigoise, Rougette, Chemlel and Dathier. The morphological characterizations (quantitative and qualitative), relating to the leaves, fruits and cores, described in the descriptors of the International Olive Council (IOC, 2003), revealed differences between the varieties. The set of morphological traits assessed during the three years of study (2013-2014- 2015) revealed a significant difference between the varieties studied confirmed by coefficients of
variation ranging from 2.81% to 39.14%. Positive correlations between mean precipitation, duration of insolations and morphological characters ranged from r = 0.839 to 0.946, and negative for fruit width and nuclei with temperature.
The results of the analysis of the biochemical characterization data revealed a great difference between the varieties. The most important lipid content was recorded in Sigoise leaf and fruit, Rougette gave the best sugar content in the leaves, as well as in Chemlel fruits. The qualitative analysis performed by phytochemical screening revealed the presence of several families of natural compounds in both organs. Rougette has been the dominant variety for total leaf and fruit phenol and flavonoid content, and Dathier for tannins. Quantitative analyzes of the phenolic compounds identified in the hydromethanol extracts by HPLC identified four
compounds: fumaric acid, gallic acid, oleuropein and caffeic acid, seven compounds in the leaves: fumaric acid, gallic acid, oleuropein, 4-hydroxybenzoic acid acid, caffeic acid, ellagic acid and t-3 hydroxyroamic acid, with a significant dominance of Oleuropein.
The evaluation of the biological potential of leaf and fruit extracts, revealed a significant anticoagulant activity in Chemlel for both organs and an antioxidant power of interest in Rougette leaves and Sigois fruits. All extracts have an important antibacterial power, extracts of leaf at Rougette gave a better zone of inhibition against S. aureus.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HIM7315.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10931 Comportements biologique, physiologique, biochimique et l’activité biologique de quatre variétés d’olivier (Olea europeae L.) dans l’Est Algérien. [texte imprimé] / Sara Himour ép Cherrier, Auteur ; Abdelouahab Yahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 209 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Olea europaea L. morphologie physiologie HPLC Oleuropéine activité biologique antioxydante anticoagulante antibactérienne morphology physiology Oleuropein biological activity antioxidant anticoagulant antibacterial اولیااوروبیا Lمورفولوجیا، فیزیولوجیة HPLCأولیوروبین النشاط البیولوجي مضادات الأكسدة مضاد للتخثر مضاد
للبكتیریاIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The present study focuses on the biological, physiological and biochemical behavior of the cultivated olive tree, Olea europaea L., represented by four varieties sampled at the Maazouzi Lakhdar station, Mila: Sigoise, Rougette, Chemlel and Dathier. The morphological characterizations (quantitative and qualitative), relating to the leaves, fruits and cores, described in the descriptors of the International Olive Council (IOC, 2003), revealed differences between the varieties. The set of morphological traits assessed during the three years of study (2013-2014- 2015) revealed a significant difference between the varieties studied confirmed by coefficients of
variation ranging from 2.81% to 39.14%. Positive correlations between mean precipitation, duration of insolations and morphological characters ranged from r = 0.839 to 0.946, and negative for fruit width and nuclei with temperature.
The results of the analysis of the biochemical characterization data revealed a great difference between the varieties. The most important lipid content was recorded in Sigoise leaf and fruit, Rougette gave the best sugar content in the leaves, as well as in Chemlel fruits. The qualitative analysis performed by phytochemical screening revealed the presence of several families of natural compounds in both organs. Rougette has been the dominant variety for total leaf and fruit phenol and flavonoid content, and Dathier for tannins. Quantitative analyzes of the phenolic compounds identified in the hydromethanol extracts by HPLC identified four
compounds: fumaric acid, gallic acid, oleuropein and caffeic acid, seven compounds in the leaves: fumaric acid, gallic acid, oleuropein, 4-hydroxybenzoic acid acid, caffeic acid, ellagic acid and t-3 hydroxyroamic acid, with a significant dominance of Oleuropein.
The evaluation of the biological potential of leaf and fruit extracts, revealed a significant anticoagulant activity in Chemlel for both organs and an antioxidant power of interest in Rougette leaves and Sigois fruits. All extracts have an important antibacterial power, extracts of leaf at Rougette gave a better zone of inhibition against S. aureus.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HIM7315.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10931 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HIM/7315 HIM/7315 Thèse Bibliothèque principale Thèses Disponible
Titre : Densité électronique et propriétés biologiques de nouveaux ligands à base de triazole et de leurs complexes d’Argent. Type de document : texte imprimé Auteurs : Zakaria Bouhidel, Auteur ; Aouatef Cherouana, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 156 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Diffraction des rayons X surfaces d’Hirshfeld densité de charge activité biologique الأشعة السينية سطوح هيرشفلد الكثافة الشحنية قدرة التثبيط Index. décimale : 540 Chimie et sciences connexes Résumé : Cinq nouveaux composés ont été synthétisés : trois ligands organiques et deux complexes d’argent. Ces composés sont caractérisés par des analyses préliminaires et en utilisant IR, UVVis . Les structures cristallines ont été déterminées à partir des données de diffraction des rayons X à basse température et à haute résolution. Le pouvoir inhibiteur de ces composés a été examiné avec la méthode de diffusion en milieu gélosé. Les résultats obtenus montrent que les ligands libres possèdent un pouvoir inhibiteur très faible voire nulle par rapport à leurs complexes d’argent. Dans le but d’examiner l’influence de la substitution des groupements hydroxyles par les atomes de chlore, nous avons mené une étude comparative de la densité de charge entre les deux ligands (L2) et (L3). Cette étude nous a permis de mieux comprendre les mécanismes de complexation de ces composés dans le but d’optimiser les différentes approches d’élaboration.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7336.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10986 Densité électronique et propriétés biologiques de nouveaux ligands à base de triazole et de leurs complexes d’Argent. [texte imprimé] / Zakaria Bouhidel, Auteur ; Aouatef Cherouana, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 156 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Diffraction des rayons X surfaces d’Hirshfeld densité de charge activité biologique الأشعة السينية سطوح هيرشفلد الكثافة الشحنية قدرة التثبيط Index. décimale : 540 Chimie et sciences connexes Résumé : Cinq nouveaux composés ont été synthétisés : trois ligands organiques et deux complexes d’argent. Ces composés sont caractérisés par des analyses préliminaires et en utilisant IR, UVVis . Les structures cristallines ont été déterminées à partir des données de diffraction des rayons X à basse température et à haute résolution. Le pouvoir inhibiteur de ces composés a été examiné avec la méthode de diffusion en milieu gélosé. Les résultats obtenus montrent que les ligands libres possèdent un pouvoir inhibiteur très faible voire nulle par rapport à leurs complexes d’argent. Dans le but d’examiner l’influence de la substitution des groupements hydroxyles par les atomes de chlore, nous avons mené une étude comparative de la densité de charge entre les deux ligands (L2) et (L3). Cette étude nous a permis de mieux comprendre les mécanismes de complexation de ces composés dans le but d’optimiser les différentes approches d’élaboration.
Diplôme : Doctorat En ligne : ../theses/chimie/BOU7336.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10986 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/7336 BOU/7336 Thèse Bibliothèque principale Thèses Disponible
Titre : Modélisation de nouvelles molécules biologiques actives liées au strss oxydant Type de document : texte imprimé Auteurs : Adel Krid ; M. Bencharif, Directeur de thèse ; Univ. de Constantine, Éditeur scientifique Année de publication : 2008 Importance : 73 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Docking moléculaire Activité biologique Liaison hydrogène Intéraction protéine-ligand Energie d'intéraction Acétylcholinestérase Chalcone Site anionique Intéractionhydrophobique Biodisponibilité Index. décimale : 540 Chimie et sciences connexes En ligne : ../theses/chimie/KRI5162.pdf Permalink : index.php?lvl=notice_display&id=3669 Modélisation de nouvelles molécules biologiques actives liées au strss oxydant [texte imprimé] / Adel Krid ; M. Bencharif, Directeur de thèse ; Univ. de Constantine, Éditeur scientifique . - 2008 . - 73 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Docking moléculaire Activité biologique Liaison hydrogène Intéraction protéine-ligand Energie d'intéraction Acétylcholinestérase Chalcone Site anionique Intéractionhydrophobique Biodisponibilité Index. décimale : 540 Chimie et sciences connexes En ligne : ../theses/chimie/KRI5162.pdf Permalink : index.php?lvl=notice_display&id=3669 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KRI/5162 KRI/5162 Thèse Bibliothèque principale Thèses Disponible
Titre : Etudes phytochimique et pharmacologique d’une plante saharienne et d’espèces de la région des Aurès. Type de document : texte imprimé Auteurs : Hamza Fadel, Auteur ; Samir Benayache, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 255 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Pulicaria inuloides Cistus albidus les Aurès fractionnement bioguidé activité biologique flavonoïdes huiles essentielles ICP-AES et ICP-MS Aures bioguided fractionation biological activity flavonoids essential oils Pulicaria inuloidesالأوراس الفعالية البيولوجية الفصل الموجه بيولوجيا الزيوت العطرية التحليل الطيفي للانبعاث الذري ) (ICP-AESالتحليل الطيفي الكتلي )(. ICP-M" Index. décimale : 540 Chimie et sciences connexes Résumé : Our goal in this work was to search the secondary metabolites of flavonoids type from two species from Asteraceae and Cistaceae families in order to search new natural compounds of biological interest. Thirteen (13) extracts from different species including twelve (12) from the Aures regions were subjected to a phytochemical screening. On the basis of these preliminary results, the phytochemical investigation of the aerial parts two species, Pulicaria inuloides and Cistus albidus from the Bechar and the Aures regions respectively was carried out. This approach allowed the isolation of twelve natural compounds, among which eleven flavonoids, one phenolic compound as well as two acetyl derivatives of one of the isolated compounds were characterized using spectroscopic methods such as UV-Visible, IR and NMR.
The biological activities in vitro (antioxidant and antibacterial activity) as well as the total phenolic and flavonoids compounds of the various extracts were discussed. We also reported the evaluation of the in vitro antiprotozoal activity of the two species Pulicaria inuloides and Cistus albidus against Leishmania amazonensis, Trypanosoma cruzi and Acanthamoeba castellanii str. Neff. The bioguided fractionation of the CHCl3 extract from the aerial part of P. inuloides leads to the isolation and characterization of quercetagetin-3,5,7,3'-tetramethylether as the active ingredient. Two acetylated derivatives were prepared from this natural compound and these three compounds were evaluated for their activities against the promastigote form of L. amazonensis.
All these compounds exhibited moderate leishmanicidal activity with IC50 values ranging from 0.234 to 0.484 mM and low toxicity to macrophage cells (CC50 ranging from 0.365 to 0.664 mM). This study reported the antiprotozoal evaluation of Pulicaria inuloides for the first time and the results show that this species can be considered as a source of anti-leishmanicidal agents. The bioguided fractionation of the active extract of CHCl3 from C. albidus against T. cruzi led to the isolation of two fractions F6,4 and F6,5 which showed also a potent activity up to 100 μg/ml. The bioguided fractionation of the active extract of AcOEt from the aerial part of C. albidus against A. castellanii led to the isolation of a fraction F14A which showed a significant activity up to 100 μg / ml. Fractions F6,4 ; F6,5 and F14A consists of product mixtures, which have not been separated.
We have also described the chemical compositions of the essential oils of Pulicaria inuloides and Cistus albidus. The major compounds of the species C. albidus are manool and 15,16-dinorlabd-8(20)- en-13-one whose skeleton of type labdane is a specific marker in this genus, these compounds are not described in samples from other regions and in particular in the Mediterranean regions (Spain and France) that appeared in the literature. The major compounds of the species Pulicaria inuloides are: δ- cadinene, α-muurolene, γ-cadinene, α-epi-cadinol and α-cadinol are not present in the other samples of Pulicaria inuloides from other areas published in the literature.
In this study, the mineral content (inorganic elements) of 21 isolated extracts of various aromatic species of the Sahara (P. inuloides) and the Aures region (Euphorbia helioscopia L., Peganum harmala L., Daphne gnidium L., Calendula algeriensis L., Bunium incrassatum L., Echinops ritro L. Marrubium deserti de Noè., Juniperus oxycedrus L., Juniperus phoenicea L., Marrubium vulgare L., Cedrus atlantica (Endl.) Manetti and Thymus algeriensis L.) were also determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and inductively coupled plasma mass spectroscopy (ICP-MS) . The results obtained showed higher concentrations of Ca, K, Mg, Na and S
(mg/kg) in the different plant extracts.
Diplôme : Doctorat En ligne : ../theses/chimie/FAD7287.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10902 Etudes phytochimique et pharmacologique d’une plante saharienne et d’espèces de la région des Aurès. [texte imprimé] / Hamza Fadel, Auteur ; Samir Benayache, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 255 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Pulicaria inuloides Cistus albidus les Aurès fractionnement bioguidé activité biologique flavonoïdes huiles essentielles ICP-AES et ICP-MS Aures bioguided fractionation biological activity flavonoids essential oils Pulicaria inuloidesالأوراس الفعالية البيولوجية الفصل الموجه بيولوجيا الزيوت العطرية التحليل الطيفي للانبعاث الذري ) (ICP-AESالتحليل الطيفي الكتلي )(. ICP-M" Index. décimale : 540 Chimie et sciences connexes Résumé : Our goal in this work was to search the secondary metabolites of flavonoids type from two species from Asteraceae and Cistaceae families in order to search new natural compounds of biological interest. Thirteen (13) extracts from different species including twelve (12) from the Aures regions were subjected to a phytochemical screening. On the basis of these preliminary results, the phytochemical investigation of the aerial parts two species, Pulicaria inuloides and Cistus albidus from the Bechar and the Aures regions respectively was carried out. This approach allowed the isolation of twelve natural compounds, among which eleven flavonoids, one phenolic compound as well as two acetyl derivatives of one of the isolated compounds were characterized using spectroscopic methods such as UV-Visible, IR and NMR.
The biological activities in vitro (antioxidant and antibacterial activity) as well as the total phenolic and flavonoids compounds of the various extracts were discussed. We also reported the evaluation of the in vitro antiprotozoal activity of the two species Pulicaria inuloides and Cistus albidus against Leishmania amazonensis, Trypanosoma cruzi and Acanthamoeba castellanii str. Neff. The bioguided fractionation of the CHCl3 extract from the aerial part of P. inuloides leads to the isolation and characterization of quercetagetin-3,5,7,3'-tetramethylether as the active ingredient. Two acetylated derivatives were prepared from this natural compound and these three compounds were evaluated for their activities against the promastigote form of L. amazonensis.
All these compounds exhibited moderate leishmanicidal activity with IC50 values ranging from 0.234 to 0.484 mM and low toxicity to macrophage cells (CC50 ranging from 0.365 to 0.664 mM). This study reported the antiprotozoal evaluation of Pulicaria inuloides for the first time and the results show that this species can be considered as a source of anti-leishmanicidal agents. The bioguided fractionation of the active extract of CHCl3 from C. albidus against T. cruzi led to the isolation of two fractions F6,4 and F6,5 which showed also a potent activity up to 100 μg/ml. The bioguided fractionation of the active extract of AcOEt from the aerial part of C. albidus against A. castellanii led to the isolation of a fraction F14A which showed a significant activity up to 100 μg / ml. Fractions F6,4 ; F6,5 and F14A consists of product mixtures, which have not been separated.
We have also described the chemical compositions of the essential oils of Pulicaria inuloides and Cistus albidus. The major compounds of the species C. albidus are manool and 15,16-dinorlabd-8(20)- en-13-one whose skeleton of type labdane is a specific marker in this genus, these compounds are not described in samples from other regions and in particular in the Mediterranean regions (Spain and France) that appeared in the literature. The major compounds of the species Pulicaria inuloides are: δ- cadinene, α-muurolene, γ-cadinene, α-epi-cadinol and α-cadinol are not present in the other samples of Pulicaria inuloides from other areas published in the literature.
In this study, the mineral content (inorganic elements) of 21 isolated extracts of various aromatic species of the Sahara (P. inuloides) and the Aures region (Euphorbia helioscopia L., Peganum harmala L., Daphne gnidium L., Calendula algeriensis L., Bunium incrassatum L., Echinops ritro L. Marrubium deserti de Noè., Juniperus oxycedrus L., Juniperus phoenicea L., Marrubium vulgare L., Cedrus atlantica (Endl.) Manetti and Thymus algeriensis L.) were also determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and inductively coupled plasma mass spectroscopy (ICP-MS) . The results obtained showed higher concentrations of Ca, K, Mg, Na and S
(mg/kg) in the different plant extracts.
Diplôme : Doctorat En ligne : ../theses/chimie/FAD7287.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10902 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité FAD/7287 FAD/7287 Thèse Bibliothèque principale Thèses Disponible
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