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Auteur Douadi Khelifi |
Documents disponibles écrits par cet auteur (13)
Affiner la recherche Interroger des sources externesContribution à la connaissance des stress biotiques affectant les céréales d'hiver / Ammar Ouffroukh
Titre : Contribution à la connaissance des stress biotiques affectant les céréales d'hiver : « Identification et approche à l'étude épidémiologique du virus de la jaunisse nanisante de L’Orge (VJNO) ou (BYDV) sévissant dans les cultures des céréales dans les zones Est de l’Algérie ». Type de document : texte imprimé Auteurs : Ammar Ouffroukh, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 111 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Céréales Maladies Virus BYDV Sérologie ELISA souches Pathotype (Pav, Mav, Rpv,) Pucerons stress hydrique Epidémiologie Cereal diseases viruses Serology strains Aphids water stress Epidemiology الحبوب الأمراض الفيروسات الأمصال السلالات أنثير طائرات المن ندرة المياه علم الأوبئة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Research on cereals in recent years, Indicate the risk of epidemic development of certain fungal diseases (rust yellow blotch, Septoria, etc.), and viral adequate measures of prevention and intervention are not taken in time (A.Ouffroukh. 2008; A.Ouffroukh, 2006). Crop losses arising are important. In favorable years the Yellow rust, for example, can take extents epidemic and destroy entire harvests. (A.Ouffroukh., 2008; A.Ouffroukh., 2006; B. Bahri, M. Leconte, A. Ouffroukh, C. Vallavieille-Pope, J. Enjalbert, 2009). Viral diseases in turn, are taking extension. Thus, the Barley Yellow Dwarf Virus (BYDV.) or (BYDV) was reported in several areas prospected and several cereal species (wheat and barley hard and soft).
In general very little information is available on their epidemiology and economic impact, when the means of struggle, they have little or almost nonexistent (H. Stone and JP Signoret, 1978). BYDV (BYDV) little known in Algeria compared to other diseases, can be very important for our cereal and threatening, particularly since this disease is transmitted by insect vectors belonging to the genus including Aphidideae, including a dozen some species have been identified (A.Ouffroukh., 2006; L. Ayadi K. and Ayoub, 1994; Ouffroukh. A Khelifi. D, Dehimet. L, 2009). Also after his observation and detection, identity veered ¬ was confirmed by serological investigations have shown that the existence of two viral strains or Pathotypes (PAV and MAV), confirming the results obtained by H. Belkahla.et Lapierre (1999).
The frequency of the disease varies annually from 8% to 18% depending on the region but is also encountered and generally on the different species at rates ranging from 8% to 28%. (L. Ayadi K. and Ayoub, 1994; A. Ouffroukh., 2008; Ouffroukh. A Khelifi. D, Dehimet. L, 2009).
In terms of capacity vector, PR appears to be best vector and infected plants by this species appear most affected clinically, about the impact of contamination on cereal species studied, it appears that the barley species are most affected, while soft wheats are more susceptible to BYDV (BYDV) and durum wheats.
Moreover, the influence of virus Barley yellow dwarf (BYDV) on cereal development is certain and it is especially important that the plant is subjected to water stress, it's more 'is that cultivars and genotypes most tolerant in general show a certain level of tolerance to drought.
In epidemiological terms, and although the results we have achieved can not definitively conclude on the precise conditions of the regions studied, the fact remains that valuable information was obtained. It appears here the whole point estimates of aphid that are a fundamental element .indication of risk to crops and therefore the periods in which the actions of prevention and protection should be carried out.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/OUF6463.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9503 Contribution à la connaissance des stress biotiques affectant les céréales d'hiver : « Identification et approche à l'étude épidémiologique du virus de la jaunisse nanisante de L’Orge (VJNO) ou (BYDV) sévissant dans les cultures des céréales dans les zones Est de l’Algérie ». [texte imprimé] / Ammar Ouffroukh, Auteur ; Douadi Khelifi, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 111 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Céréales Maladies Virus BYDV Sérologie ELISA souches Pathotype (Pav, Mav, Rpv,) Pucerons stress hydrique Epidémiologie Cereal diseases viruses Serology strains Aphids water stress Epidemiology الحبوب الأمراض الفيروسات الأمصال السلالات أنثير طائرات المن ندرة المياه علم الأوبئة Index. décimale : 570 Sciences de la vie. Biologie Résumé : Research on cereals in recent years, Indicate the risk of epidemic development of certain fungal diseases (rust yellow blotch, Septoria, etc.), and viral adequate measures of prevention and intervention are not taken in time (A.Ouffroukh. 2008; A.Ouffroukh, 2006). Crop losses arising are important. In favorable years the Yellow rust, for example, can take extents epidemic and destroy entire harvests. (A.Ouffroukh., 2008; A.Ouffroukh., 2006; B. Bahri, M. Leconte, A. Ouffroukh, C. Vallavieille-Pope, J. Enjalbert, 2009). Viral diseases in turn, are taking extension. Thus, the Barley Yellow Dwarf Virus (BYDV.) or (BYDV) was reported in several areas prospected and several cereal species (wheat and barley hard and soft).
In general very little information is available on their epidemiology and economic impact, when the means of struggle, they have little or almost nonexistent (H. Stone and JP Signoret, 1978). BYDV (BYDV) little known in Algeria compared to other diseases, can be very important for our cereal and threatening, particularly since this disease is transmitted by insect vectors belonging to the genus including Aphidideae, including a dozen some species have been identified (A.Ouffroukh., 2006; L. Ayadi K. and Ayoub, 1994; Ouffroukh. A Khelifi. D, Dehimet. L, 2009). Also after his observation and detection, identity veered ¬ was confirmed by serological investigations have shown that the existence of two viral strains or Pathotypes (PAV and MAV), confirming the results obtained by H. Belkahla.et Lapierre (1999).
The frequency of the disease varies annually from 8% to 18% depending on the region but is also encountered and generally on the different species at rates ranging from 8% to 28%. (L. Ayadi K. and Ayoub, 1994; A. Ouffroukh., 2008; Ouffroukh. A Khelifi. D, Dehimet. L, 2009).
In terms of capacity vector, PR appears to be best vector and infected plants by this species appear most affected clinically, about the impact of contamination on cereal species studied, it appears that the barley species are most affected, while soft wheats are more susceptible to BYDV (BYDV) and durum wheats.
Moreover, the influence of virus Barley yellow dwarf (BYDV) on cereal development is certain and it is especially important that the plant is subjected to water stress, it's more 'is that cultivars and genotypes most tolerant in general show a certain level of tolerance to drought.
In epidemiological terms, and although the results we have achieved can not definitively conclude on the precise conditions of the regions studied, the fact remains that valuable information was obtained. It appears here the whole point estimates of aphid that are a fundamental element .indication of risk to crops and therefore the periods in which the actions of prevention and protection should be carried out.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/OUF6463.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9503 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OUF/6463 OUF/6463 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intégraleAdobe Acrobat PDF
Titre : Criblage des plantes médicinales utilisées dans la région de Constantine-Mila et valorisation phytochimique des plantes : Bellevalia mauritanica Pomel ; Endémique de l’Afrique du Nord et Dittrichia viscosa (L.) Greuter. Type de document : texte imprimé Auteurs : Rayene Ouelbani, Auteur ; Douadi Khelifi, Directeur de thèse ; Toma Nardjes Mouas, Directeur de thèse Mention d'édition : 25/10/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 174 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire : Biochimie Appliquée Ethnobotanique Bellevalia mauritanica Pomel LC-MS/MS HPLC-MS-ITTOF activités biologiques Ethnobotany biological activities Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The privileged geographical situation of Algeria confers a great floristic richness which represents an asset for phytochemistry, moreover until our days the Algerian population resorts to the traditional practices using plants to cure themselves. The present work is divided into two parts, the first one devoted to an ethnobotanical
investigation on the medicinal plants used in the region of Constantine and Mila and the second one includes the phytochemical study of the endemic North African plant Bellevalia mauritanica Pomel, as well as the evaluation of its biological activities in vitro. The ethnobotanical investigation of the region of Constantine and Mila, allowed us to obtain the following results: out of 102 plants mentioned, 28 new species, 31 common plants with 151 new therapeutic applications, in comparison with other regions in Algeria were mentioned and 12 new species including an endemic plant Zygophyllum cornutum Coss., 75 known plants with 369 new medicinal uses, in comparison with other regions of the Mediterranean basin. The phytochemical study of Bellevalia mauritanica Pomel by liquid chromatography coupled to a hybrid ion trap and time-of-flight mass spectrometer (LC-MS-IT-TOF) indicated the presence of 91 compounds in the different phases of the crude extract of the underground part and a validated method of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), using 37 standards revealed the content of phenolic acids rather than flavonoids, in fact the AcOEt phase contains caffeic acid (1240.7 µg/g extract) and the CHCl3 phase contains vanillin (2048.5 µg/g extract) in high amounts, as well as the evaluation of its Antioxidant, anticholinesterase and antibacterial in vitro activities showed that among the tested extracts, the AcOEt phase shows good antioxidant activity, especially in ABTS•+ (2, 2'- azinobis-(3-ethylbenzothiazoline-6-sulfonic), DPPH• (1,1-diphenyl-2-picrylhydrazyl) and DMSO (dimethylsulfoxide) alkaline superoxide anion, considerable inhibition of acetylcholinesterase and moderate antibacterial effect.
Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/OUE7828.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11660 Criblage des plantes médicinales utilisées dans la région de Constantine-Mila et valorisation phytochimique des plantes : Bellevalia mauritanica Pomel ; Endémique de l’Afrique du Nord et Dittrichia viscosa (L.) Greuter. [texte imprimé] / Rayene Ouelbani, Auteur ; Douadi Khelifi, Directeur de thèse ; Toma Nardjes Mouas, Directeur de thèse . - 25/10/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 174 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire : Biochimie Appliquée Ethnobotanique Bellevalia mauritanica Pomel LC-MS/MS HPLC-MS-ITTOF activités biologiques Ethnobotany biological activities Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The privileged geographical situation of Algeria confers a great floristic richness which represents an asset for phytochemistry, moreover until our days the Algerian population resorts to the traditional practices using plants to cure themselves. The present work is divided into two parts, the first one devoted to an ethnobotanical
investigation on the medicinal plants used in the region of Constantine and Mila and the second one includes the phytochemical study of the endemic North African plant Bellevalia mauritanica Pomel, as well as the evaluation of its biological activities in vitro. The ethnobotanical investigation of the region of Constantine and Mila, allowed us to obtain the following results: out of 102 plants mentioned, 28 new species, 31 common plants with 151 new therapeutic applications, in comparison with other regions in Algeria were mentioned and 12 new species including an endemic plant Zygophyllum cornutum Coss., 75 known plants with 369 new medicinal uses, in comparison with other regions of the Mediterranean basin. The phytochemical study of Bellevalia mauritanica Pomel by liquid chromatography coupled to a hybrid ion trap and time-of-flight mass spectrometer (LC-MS-IT-TOF) indicated the presence of 91 compounds in the different phases of the crude extract of the underground part and a validated method of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), using 37 standards revealed the content of phenolic acids rather than flavonoids, in fact the AcOEt phase contains caffeic acid (1240.7 µg/g extract) and the CHCl3 phase contains vanillin (2048.5 µg/g extract) in high amounts, as well as the evaluation of its Antioxidant, anticholinesterase and antibacterial in vitro activities showed that among the tested extracts, the AcOEt phase shows good antioxidant activity, especially in ABTS•+ (2, 2'- azinobis-(3-ethylbenzothiazoline-6-sulfonic), DPPH• (1,1-diphenyl-2-picrylhydrazyl) and DMSO (dimethylsulfoxide) alkaline superoxide anion, considerable inhibition of acetylcholinesterase and moderate antibacterial effect.
Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/OUE7828.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11660 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OUE/7828 OUE/7828 Thèse Bibliothèque principale Thèses Disponible
Titre : Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique Type de document : texte imprimé Auteurs : Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 211 f. Format : 30 cm Note générale : Doctorat 3ème cycle
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis القمح؛التنوع؛ بروتينات؛الهجرة الكهربائية للبروتينات؛حساسية للقمح؛المضادات الجسمية للمرضى من نوع ؛"IgE"بروتينات القمح مسببة الحساسية؛التحليل البروتيومي؛ Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9422 Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique [texte imprimé] / Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 211 f. ; 30 cm.
Doctorat 3ème cycle
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis القمح؛التنوع؛ بروتينات؛الهجرة الكهربائية للبروتينات؛حساسية للقمح؛المضادات الجسمية للمرضى من نوع ؛"IgE"بروتينات القمح مسببة الحساسية؛التحليل البروتيومي؛ Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9422 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/6405 BEL/6405 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intègraleAdobe Acrobat PDF
Titre : Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire Type de document : texte imprimé Auteurs : Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 132 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins الجزائر عينة التنوع الوراثي مورفولوجيا غلوتينين غليادين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10269 Diversité génétique de l'espèce Aegilops geniculata Roth en Algérie par approches morphologique, biochimique et moléculaire [texte imprimé] / Asma Medouri, Auteur ; Douadi Khelifi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 132 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Aegilops geniculata Accession Algérie Diversité Morphologie Gluténines Gliadines RAPD Algeria Diversity Morphology Glutenins Gliadins الجزائر عينة التنوع الوراثي مورفولوجيا غلوتينين غليادين Index. décimale : 570 Sciences de la vie. Biologie Résumé : The study of genetic diversity in a collection of the allotetraploid species Aegilops geniculata Roth(UM) collected in Algeria was assessed using morphological, biochemical and molecular approaches.
This was carried on a wide sampling following a climatic gradient throughout North Algeria. This estimation was preceded by assessing the genetic variability of the diploid progenitors of the tetraploid species (Ae. comosa and Ae. umbellulata) based on gluten proteins. For diploids, a significant variation
was revealed for the three classes of gluten proteins. Several new alleles corresponding to HMW-GS were identified and a nomenclature was proposed as: 8 alleles were found at the Glu-M1 locus (with 5 new alleles) and 3 alleles (with one new) at the Glu-U1 locus. For LMW-GS (B- zone), 14 and 7 alleles were revealed at Glu-M3 and Glu-U3 loci respectively. For gliadins, a typology was established.
Morphological diversity of Ae. geniculata was estimated using 15 descriptors. Individuals were morphologically homogenous and little differences were found between accessions. Results of PCA reveal that traits expressing the wide of the spike distinguished better between accessions. Biochemical analysis of glutenins showed a high level of polymorphism as; 15 alleles were recognized at the GluM1 locus and 12 at the Glu-U1 locus with a new subunit and several new alleles. For B-LMW-GS,
the locus Glu-M3 expressed 28 alleles and the locus Glu-U3 expressed 25 alleles. Thirty-two specific patterns were revealed for C-LMW-GS. With respect to gliadins, 61 polymorphic bands resulting in 35 gliadin blocks were found. The genetic diversity index indicated that ω-gliadins were the most polymorphic, followed by γ- and β-gliadin then α-gliadins. Molecular data based on RAPD showed a high polymorphism as 145 fragments were detected with a level of polymorphism of 79.31%.
The three studied approaches (morphology, proteins and DNA polymorphism) showed relationships between genetic variation of the accessions and ecogeographic conditions. Correlation between morphology and some climatic factors showed that some independent characters were correlated with the winter minimum temperature. The polymorphism detected in gliadins highlighted a strong correlation between ecogeographic parameters and some ω-gliadin bands especially the band B14.
RAPD polymorphism showed a strong correlation with ecological parameters."
Diplôme : Doctorat En ligne : ../theses/biologie/MED6917.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10269 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MED/6917 MED/6917 Thèse Bibliothèque principale Thèses Disponible
Titre : Diversité des sous-unités gluténines HPM et FPM, sous-unités sécalines HPM et 75Kgamme-sécalines d'une collection de triticales hexzploïdes Type de document : texte imprimé Auteurs : Sihem Amri, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2011 Importance : 96 f. Format : 31 cm Note générale : Magister
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Diversité triticale hexaploïde gluténines, sécalines HPM FPM SDS-PAGE. Biologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/AMR5908.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=5708 Diversité des sous-unités gluténines HPM et FPM, sous-unités sécalines HPM et 75Kgamme-sécalines d'une collection de triticales hexzploïdes [texte imprimé] / Sihem Amri, Auteur ; Douadi Khelifi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2011 . - 96 f. ; 31 cm.
Magister
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Diversité triticale hexaploïde gluténines, sécalines HPM FPM SDS-PAGE. Biologie Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/AMR5908.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=5708 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité AMR/5908 AMR/5908 Thèse Bibliothèque principale Thèses Disponible
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