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Partager le résultat de cette recherche Interroger des sources externesBiodiversité des actinomycètes halophiles et halotolérants isolés à partir de trois sites Ramsar situés au niveau de la Wilaya d’Oum El Bouaghi. / Chamss eddine Djaballah
Titre : Biodiversité des actinomycètes halophiles et halotolérants isolés à partir de trois sites Ramsar situés au niveau de la Wilaya d’Oum El Bouaghi. Type de document : texte imprimé Auteurs : Chamss eddine Djaballah, Auteur ; Mahmoud Kitouni, Auteur Mention d'édition : 28/01/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 175 f. Format : 30 cm. Note générale : 1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Microbiologie: Ecologie Microbiennes biodiversité culturomics Streptomyces massilialgeriensis sp. nov. actinomycètes Ramsar écosystème extrême biodiversity Actinomycetes extreme ecosystem التنوع البيولوجي الأكتينوميسيت رامسار البيئة القاسية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This work represents a first study of the biodiversity of the actinomycetes inhabitants three Sebkhas protected by the Ramsar-Algeria convention. The physicochemical analyzes of the soil samples revealed extremely high salinities, basic or very basic pH, low percentages of moisture, moderate or very high percentages of organic matter. These results also showed that samples of sebkha Annk Djemel belong to the ""clay"" and ""Clay loam"" classes, samples of sebkha Guellif belong to the ""clay loam"" and ""sandy-clay loam"" classes and samples of sebkha Et Taref belong to the ""clay"" class. An isolation of actinomycetes from the soil has been achieved by the use of conventional methods and / or Culturomics. The enumeration results revealed that the number of actinomycetes could reach to
2.105 CFU/g of soil. Based on the enumeration results, a statistical analysis, carried out by the application of non-parametric tests of Kruskal-Wallis and Mann-Whitney, revealed that culture media relatively poor in nutrients and the concentration 5% of NaCl are the most favorable in the isolation of actinomycetes. The competitive effect of bacterial and fungal flora has also been highlighted. Following the isolation protocols adopted, we were able to purify and store a total of 204 isolates, all Gram (+), of which 108 isolates were derived from conventional isolation techniques and 96 isolates were obtained by the Culturomics method. Identification by the MALDITOF MS and sequencing of the 16S rDNA methods allowed us to identify a new species that we named Streptomyces massilialgeriensis sp. nov. and to assign certain other isolates to the genera Streptomyces, Nocardiopsis and Actinomyces. Other isolates have been identified as Bacillus, Virgibacillus, Gracilibacillus, Staphylococcus, Flavonifactor and Halobacillus. The activity test, carried out by the agar plug diffusion method, of 13 selected isolates belonging to the genera Streptomyces and Nocardiopsis against multidrug-resistant bacteria reveal interesting zones of inhibition of the isolate S20 against K. pneumoniae CSUR P1596 (coli R), E. coli CSUR P4969 (coli R, HLP), E. coli CSUR P5139 (carba R) and P. mirabilis CSUR P2049 (oxa-48), and isolate S112
against S. aureus CSUR P1943 (MRSA, KTG, inducible MLSB), E. coli CSUR P4969 (coli R, HLP) and P. mirabilis CSUR P2049 (oxa-48).
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/DJA7814.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11646 Biodiversité des actinomycètes halophiles et halotolérants isolés à partir de trois sites Ramsar situés au niveau de la Wilaya d’Oum El Bouaghi. [texte imprimé] / Chamss eddine Djaballah, Auteur ; Mahmoud Kitouni, Auteur . - 28/01/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 175 f. ; 30 cm.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Microbiologie: Ecologie Microbiennes biodiversité culturomics Streptomyces massilialgeriensis sp. nov. actinomycètes Ramsar écosystème extrême biodiversity Actinomycetes extreme ecosystem التنوع البيولوجي الأكتينوميسيت رامسار البيئة القاسية Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This work represents a first study of the biodiversity of the actinomycetes inhabitants three Sebkhas protected by the Ramsar-Algeria convention. The physicochemical analyzes of the soil samples revealed extremely high salinities, basic or very basic pH, low percentages of moisture, moderate or very high percentages of organic matter. These results also showed that samples of sebkha Annk Djemel belong to the ""clay"" and ""Clay loam"" classes, samples of sebkha Guellif belong to the ""clay loam"" and ""sandy-clay loam"" classes and samples of sebkha Et Taref belong to the ""clay"" class. An isolation of actinomycetes from the soil has been achieved by the use of conventional methods and / or Culturomics. The enumeration results revealed that the number of actinomycetes could reach to
2.105 CFU/g of soil. Based on the enumeration results, a statistical analysis, carried out by the application of non-parametric tests of Kruskal-Wallis and Mann-Whitney, revealed that culture media relatively poor in nutrients and the concentration 5% of NaCl are the most favorable in the isolation of actinomycetes. The competitive effect of bacterial and fungal flora has also been highlighted. Following the isolation protocols adopted, we were able to purify and store a total of 204 isolates, all Gram (+), of which 108 isolates were derived from conventional isolation techniques and 96 isolates were obtained by the Culturomics method. Identification by the MALDITOF MS and sequencing of the 16S rDNA methods allowed us to identify a new species that we named Streptomyces massilialgeriensis sp. nov. and to assign certain other isolates to the genera Streptomyces, Nocardiopsis and Actinomyces. Other isolates have been identified as Bacillus, Virgibacillus, Gracilibacillus, Staphylococcus, Flavonifactor and Halobacillus. The activity test, carried out by the agar plug diffusion method, of 13 selected isolates belonging to the genera Streptomyces and Nocardiopsis against multidrug-resistant bacteria reveal interesting zones of inhibition of the isolate S20 against K. pneumoniae CSUR P1596 (coli R), E. coli CSUR P4969 (coli R, HLP), E. coli CSUR P5139 (carba R) and P. mirabilis CSUR P2049 (oxa-48), and isolate S112
against S. aureus CSUR P1943 (MRSA, KTG, inducible MLSB), E. coli CSUR P4969 (coli R, HLP) and P. mirabilis CSUR P2049 (oxa-48).
Note de contenu : Annexes. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/DJA7814.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=11646 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJA/7814 DJA/7814 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude écophysiologique, développement et importance des plantes médicinales du genre Mentha dans le Parc National d’El-Kala (Nord-Est Algérie). Type de document : texte imprimé Auteurs : Amina Benabdallah, Auteur ; Chaabane Rahmoune, Directeur de thèse Importance : 152 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : M. aquatica M. arvensis Mx piperita M.pulegium M. rotundifolia M. x villosa NPEK ElKala RAMSAR phenols flavonoids tannins essential oil antioxidant acetylcholinesterase PNEK phénols, flavonoïdes, huile essentielle antioxydant acétylcholinestérase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mentha species are widely used by native habitants of El-Tarf region, as condiment, flavor or
herb in culinary preparations but also used for therapeutic properties as carminative, sedative,
antispasmodic and well known to treat stomach pain.
The aim of this work was first to inventory and geolocalisate Mentha species of the National
Park of El-Kala and then to investigate the total phenolics, flavonoids and tannins content and
the in-vitro antioxidant activities of methanolic extracts and essential oils, then to evaluate the
chemical composition of essential oils obtained from aerial parts of six wildMentha species:
M. aquatica, M. arvensis, M x piperita, M. pulegium, M. rotundifolia and M x villosa.
Chemical composition was analyzed by GC-MS method and biological activities investigated
by DPPH, B-carotene bleaching and ion chelating assays for the antioxidant activity, as well
as the inhibition of acetycholinesterase. The methanol extracts of mints were rich in phenolic
compounds and exhibited a powerful antioxidant activity ranged from7.5µg/mlto44.66
µg/ml,which varied significantly among species. M. aquatica stands out with an efficient
antioxidant ability which is correlated to the high total phenolics contents, followed by M.
arvensis and M. piperita with very close values, comparing to M. pulegium, M. rotundifolia
and M. villosa with lowest values. Qualitative and quantitative variations were observed
among species. The highest yield of oils, obtained by hydrodistillation, was recorded for M.
pulegium with 1.8%. A total of 27 compounds were identified with the predominance of
oxygenated monoterpenes such as menthofurane (73.38%), rotundifolone (65.99%), pulegone
(59.12%), menthol (49.89%), menthone (20.84%), neomenthol (20.76%), 1.8-cineol (18.16%)
and β-caryophyllene (12.55%). In general, M. aquatica exhibited the strongest antioxidant
level with IC50 of 0.69±0.06 mg/ml assessed by DPPH assay, 0.16±0.02 mg/ml by β-Carotene
bleaching and 1.73±0.17 mg/ml by ferrous ion chelating method. Whereas M. arvensisstood
out with IC50 of 27.5mg/ml for acetylcholinesterase inhibitory.These results show that Mentha
species from NPEK have a great potential of polyphenols which can be used as a natural food
preservative and antioxidant source. Moreover, mints could be exploited for their
pharmacological application in order to prevent induced diseases relied to oxidative stress.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BEN7087.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10514 Etude écophysiologique, développement et importance des plantes médicinales du genre Mentha dans le Parc National d’El-Kala (Nord-Est Algérie). [texte imprimé] / Amina Benabdallah, Auteur ; Chaabane Rahmoune, Directeur de thèse . - [s.d.] . - 152 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : M. aquatica M. arvensis Mx piperita M.pulegium M. rotundifolia M. x villosa NPEK ElKala RAMSAR phenols flavonoids tannins essential oil antioxidant acetylcholinesterase PNEK phénols, flavonoïdes, huile essentielle antioxydant acétylcholinestérase Index. décimale : 570 Sciences de la vie. Biologie Résumé : Mentha species are widely used by native habitants of El-Tarf region, as condiment, flavor or
herb in culinary preparations but also used for therapeutic properties as carminative, sedative,
antispasmodic and well known to treat stomach pain.
The aim of this work was first to inventory and geolocalisate Mentha species of the National
Park of El-Kala and then to investigate the total phenolics, flavonoids and tannins content and
the in-vitro antioxidant activities of methanolic extracts and essential oils, then to evaluate the
chemical composition of essential oils obtained from aerial parts of six wildMentha species:
M. aquatica, M. arvensis, M x piperita, M. pulegium, M. rotundifolia and M x villosa.
Chemical composition was analyzed by GC-MS method and biological activities investigated
by DPPH, B-carotene bleaching and ion chelating assays for the antioxidant activity, as well
as the inhibition of acetycholinesterase. The methanol extracts of mints were rich in phenolic
compounds and exhibited a powerful antioxidant activity ranged from7.5µg/mlto44.66
µg/ml,which varied significantly among species. M. aquatica stands out with an efficient
antioxidant ability which is correlated to the high total phenolics contents, followed by M.
arvensis and M. piperita with very close values, comparing to M. pulegium, M. rotundifolia
and M. villosa with lowest values. Qualitative and quantitative variations were observed
among species. The highest yield of oils, obtained by hydrodistillation, was recorded for M.
pulegium with 1.8%. A total of 27 compounds were identified with the predominance of
oxygenated monoterpenes such as menthofurane (73.38%), rotundifolone (65.99%), pulegone
(59.12%), menthol (49.89%), menthone (20.84%), neomenthol (20.76%), 1.8-cineol (18.16%)
and β-caryophyllene (12.55%). In general, M. aquatica exhibited the strongest antioxidant
level with IC50 of 0.69±0.06 mg/ml assessed by DPPH assay, 0.16±0.02 mg/ml by β-Carotene
bleaching and 1.73±0.17 mg/ml by ferrous ion chelating method. Whereas M. arvensisstood
out with IC50 of 27.5mg/ml for acetylcholinesterase inhibitory.These results show that Mentha
species from NPEK have a great potential of polyphenols which can be used as a natural food
preservative and antioxidant source. Moreover, mints could be exploited for their
pharmacological application in order to prevent induced diseases relied to oxidative stress.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/BEN7087.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10514 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/7087 BEN/7087 Thèse Bibliothèque principale Thèses Disponible
