Résultat de la recherche
6 recherche sur le tag
'Cytotoxicity' 
Affiner la recherche Générer le flux rss de la recherche
Partager le résultat de cette recherche Interroger des sources externesIdentification polyphasique des souches d’actinobactéries isolées d’échantillons de sols semi-arides / Nassima Leulmi
Titre : Identification polyphasique des souches d’actinobactéries isolées d’échantillons de sols semi-arides : Caractérisation structurale des antibiotiques produits. Type de document : texte imprimé Auteurs : Nassima Leulmi, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 174 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Actinobactéries Streptomyces sols semi-arides antibiotiques polyéthers ionophores cytotoxicité Actinobacteria Semi-arid soils Antibiotics ionophoric polyethers Cytotoxicity الاكتينوبكتيريا ترب شبه جافة المضادات الحيوية polyéthers ionophores التسمم الخلوي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Actinobacteria are a group of filamentous bacteria, mainly inhabit the soil. They are known for producing a wide variety of bioactive molecules, especially antibiotics. Is in this context that our research work focused. A total of 99 isolates were recovered from a semi-arid soi,collected from; rhizospheric associated soil (Khenchela), mountain soil (Mount chélia region, khenchela) and Sebkha soil (Oum El bouaghi). Three strains (SF10, SFKH4 and SFKH10), were selected, based on preliminary screening for antimicrobial activity. The isolates were identified as belonging to the genus Streptomyces, based on phenotypic and chemotaxonomic study. Phylogenetic analysis revealed that the strains SF10, SFKH4 and SFKH10 were most closely related to Streptomyces youssoufiensis, Streptomyces spororaveus and Streptomyces coeruleorubidus with a pairwise 16S rRNA gene sequence similarity of 99, 8, 99 % and 99%. Ethyl acetate extracts provied by the culture of the strains on solid culture were tested for their antimicrobial activity against the test microorganisms. The structure elucidation of the biologically active molecules, purified by different chromatographic methods, was carried out by coupling several techniques of mass
spectrometry and Nuclear Magnetic Resonance (1H NMR, 13C NMR, MS, MS tandem, IR and UV-Visible). Streptomyces youssoufiensis SF10 strain produced several molecules belonging to ionophoric polyethers family, abierixin, nigericin, epinigericin, and the newly isolated grisorixin methyl ester. Moreover, only one molecule, belonging to macrotetrolides family (dinactin), was obtained from Streptomyces coeruleorubidus SFKH10 strain. In addition, the partial characterization of the molecules secreted by S. spororaveus SFKH4 strain has shown its capacity to produce a large variety of complexes compounds having high molecular weight. The presence of epimeric forms of nigericin/epinigericin and grisorixin/epigrisorixin has spurred DFT (Density Functional Theory) computational calculations. This analysis was able to provide the relative stability of the most favored epimers. This consideration is of general interest and can be applied to the wide class of naturally occurring polycyclic polyethers. On the other hand, the study of the cytotoxicity of the purified and identified molecules revealed that both nigericin and grisorixin methyl ester showed to affect glioblastoma stem cells proliferation in a dose-dependent manner, with a higher activity for the more lipophilic grisorixin methyl ester (IC50 values of 3.85 and 3.05 μM for VIPI and COMI humain glioblastoma stem cells, respectively). In conclusion, the present study has revealed the potential of semi-arid Algerian ecosystems as a good source for bioactives molecules-producing actinobacteria, specialy Streptomyces youssoufienisis SF10 strain whose secondary metabolites profile has not been studied so far by bioguided method. The clear antiproliferative activity of grisorixin methyl ester on glioblastoma stem cells represents a promising starting point aimed to the development of new drug candidates in the treatment of glioblastoma multiforme.
Diplôme : Doctorat en sciences Permalink : index.php?lvl=notice_display&id=11029 Identification polyphasique des souches d’actinobactéries isolées d’échantillons de sols semi-arides : Caractérisation structurale des antibiotiques produits. [texte imprimé] / Nassima Leulmi, Auteur ; Abderrahmane Boulahrouf, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 174 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Actinobactéries Streptomyces sols semi-arides antibiotiques polyéthers ionophores cytotoxicité Actinobacteria Semi-arid soils Antibiotics ionophoric polyethers Cytotoxicity الاكتينوبكتيريا ترب شبه جافة المضادات الحيوية polyéthers ionophores التسمم الخلوي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Actinobacteria are a group of filamentous bacteria, mainly inhabit the soil. They are known for producing a wide variety of bioactive molecules, especially antibiotics. Is in this context that our research work focused. A total of 99 isolates were recovered from a semi-arid soi,collected from; rhizospheric associated soil (Khenchela), mountain soil (Mount chélia region, khenchela) and Sebkha soil (Oum El bouaghi). Three strains (SF10, SFKH4 and SFKH10), were selected, based on preliminary screening for antimicrobial activity. The isolates were identified as belonging to the genus Streptomyces, based on phenotypic and chemotaxonomic study. Phylogenetic analysis revealed that the strains SF10, SFKH4 and SFKH10 were most closely related to Streptomyces youssoufiensis, Streptomyces spororaveus and Streptomyces coeruleorubidus with a pairwise 16S rRNA gene sequence similarity of 99, 8, 99 % and 99%. Ethyl acetate extracts provied by the culture of the strains on solid culture were tested for their antimicrobial activity against the test microorganisms. The structure elucidation of the biologically active molecules, purified by different chromatographic methods, was carried out by coupling several techniques of mass
spectrometry and Nuclear Magnetic Resonance (1H NMR, 13C NMR, MS, MS tandem, IR and UV-Visible). Streptomyces youssoufiensis SF10 strain produced several molecules belonging to ionophoric polyethers family, abierixin, nigericin, epinigericin, and the newly isolated grisorixin methyl ester. Moreover, only one molecule, belonging to macrotetrolides family (dinactin), was obtained from Streptomyces coeruleorubidus SFKH10 strain. In addition, the partial characterization of the molecules secreted by S. spororaveus SFKH4 strain has shown its capacity to produce a large variety of complexes compounds having high molecular weight. The presence of epimeric forms of nigericin/epinigericin and grisorixin/epigrisorixin has spurred DFT (Density Functional Theory) computational calculations. This analysis was able to provide the relative stability of the most favored epimers. This consideration is of general interest and can be applied to the wide class of naturally occurring polycyclic polyethers. On the other hand, the study of the cytotoxicity of the purified and identified molecules revealed that both nigericin and grisorixin methyl ester showed to affect glioblastoma stem cells proliferation in a dose-dependent manner, with a higher activity for the more lipophilic grisorixin methyl ester (IC50 values of 3.85 and 3.05 μM for VIPI and COMI humain glioblastoma stem cells, respectively). In conclusion, the present study has revealed the potential of semi-arid Algerian ecosystems as a good source for bioactives molecules-producing actinobacteria, specialy Streptomyces youssoufienisis SF10 strain whose secondary metabolites profile has not been studied so far by bioguided method. The clear antiproliferative activity of grisorixin methyl ester on glioblastoma stem cells represents a promising starting point aimed to the development of new drug candidates in the treatment of glioblastoma multiforme.
Diplôme : Doctorat en sciences Permalink : index.php?lvl=notice_display&id=11029 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LEU/7380 LEU/7380 Thèse Bibliothèque principale Thèses Disponible 
Titre : Le sapin d'Algérie (Abies numidica de Lannoy ex Carrière): étude phytochimique des métabolites secondaires et évaluation in vitro, in vivo et in silico des activités biologiques de ses composés. Type de document : texte imprimé Auteurs : Djamila Benouchenne, Auteur ; Ines Bellil, Directeur de thèse ; Douadi Khelifi, Directeur de thèse Mention d'édition : 02/12/2021 Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2021 Importance : 146 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Biochimie / Nutrition Abies numidica de Lannoy LC-MS/MS GC/MS cytotoxicité inhibition des enzymes activités biologiques toxicité in vivo métabolites secondaires cytotoxicity inhibition of
enzymes, biological activities in vivo toxicity secondary metabolites السمية الخلوية تثبيط الإنزيمات والأنشطة البيولوجية سمية في الجسم الحي المستقلبات الثانويةIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
This study aims to enhance the virtues of an Algerian endemic plant which is the Algerian fir ""Abies numidica de Lannoy ex Carrière"" by a phytochemical characterization and an evaluation of the biological activities of its leaves. The phytochemical study revealed the main secondary metabolites found in particular were polyphenols (phenolic acids, flavonoids and tannins). LCMS / MS Analysis of the polyphenolic in extracts revealed the richness of the leaves of the Algerian fir in phenolic compounds, 12 molecules were identified in the AE fraction and 8 compounds were determined in the n-BuOH extract. GC-MS analysis showed the presence of 29 terpene compounds. The evaluation of the antioxidant power in vitro by colorimetric methods (DPPH •, ABTS • +, reducing power, CUPRAC and phenanthroline) showed that the AE and n-BuOH extracts endowed with a high antioxidant power. The antimicrobial effect of the medicinal plant varies depending on the nature of the strain and the extract tested. The extracts exhibited a significant cytotoxicity against Artemia salina lethality. In vivo, the AE and n-BuOH extract from the leaves of A. numidca showed no mortality at the concentration of 2000 mg / kg. Furthermore, biochemical parameters confirmed the presence of liver damage, by increasing the concentration of liver transaminases. The in silico toxicity study confirmed that the extracts were not toxic. The present study confirmed the effectiveness of the plant and provided measurable biological proof of its antioxidant, enzyme inhibitory and antibacterial power.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN7855.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11844 Le sapin d'Algérie (Abies numidica de Lannoy ex Carrière): étude phytochimique des métabolites secondaires et évaluation in vitro, in vivo et in silico des activités biologiques de ses composés. [texte imprimé] / Djamila Benouchenne, Auteur ; Ines Bellil, Directeur de thèse ; Douadi Khelifi, Directeur de thèse . - 02/12/2021 . - جامعة الإخوة منتوري قسنطينة, 2021 . - 146 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
1 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biochimie et Biologie Cellulaire et Moléculaire: Biochimie / Nutrition Abies numidica de Lannoy LC-MS/MS GC/MS cytotoxicité inhibition des enzymes activités biologiques toxicité in vivo métabolites secondaires cytotoxicity inhibition of
enzymes, biological activities in vivo toxicity secondary metabolites السمية الخلوية تثبيط الإنزيمات والأنشطة البيولوجية سمية في الجسم الحي المستقلبات الثانويةIndex. décimale : 570 Sciences de la vie. Biologie Résumé :
This study aims to enhance the virtues of an Algerian endemic plant which is the Algerian fir ""Abies numidica de Lannoy ex Carrière"" by a phytochemical characterization and an evaluation of the biological activities of its leaves. The phytochemical study revealed the main secondary metabolites found in particular were polyphenols (phenolic acids, flavonoids and tannins). LCMS / MS Analysis of the polyphenolic in extracts revealed the richness of the leaves of the Algerian fir in phenolic compounds, 12 molecules were identified in the AE fraction and 8 compounds were determined in the n-BuOH extract. GC-MS analysis showed the presence of 29 terpene compounds. The evaluation of the antioxidant power in vitro by colorimetric methods (DPPH •, ABTS • +, reducing power, CUPRAC and phenanthroline) showed that the AE and n-BuOH extracts endowed with a high antioxidant power. The antimicrobial effect of the medicinal plant varies depending on the nature of the strain and the extract tested. The extracts exhibited a significant cytotoxicity against Artemia salina lethality. In vivo, the AE and n-BuOH extract from the leaves of A. numidca showed no mortality at the concentration of 2000 mg / kg. Furthermore, biochemical parameters confirmed the presence of liver damage, by increasing the concentration of liver transaminases. The in silico toxicity study confirmed that the extracts were not toxic. The present study confirmed the effectiveness of the plant and provided measurable biological proof of its antioxidant, enzyme inhibitory and antibacterial power.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN7855.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11844 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/7855 BEN/7855 Thèse Bibliothèque principale Thèses Disponible
Titre : Synthèse de composés hétérocycliques à visée anticancèreuse Type de document : texte imprimé Auteurs : Nora Chouha, Auteur ; Taoues Boumoud, Auteur ; Laurent Desaubry, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 155 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Triarylimidazoles spirooxindoles acide citrique BF3.OEt2 iminobenzimidazole fluorizoline oncogène KRAS cytotoxicité leucémie citric acid KRAS oncogene cytotoxicity leukemia Index. décimale : 540 Chimie et sciences connexes Résumé : Our work focuses on the development of new synthetic approaches to anticancer compounds. This work was carried out at the University of Constantine and Strasbourg, under Prof. Taoues Boumoud and Dr. Laurent Désaubry’s supervision. Triarylimidazoles and spirooxindoles are heterocycles found among several important anti-cancer agents. In order to facilitate the pharmacochemical exploration of these compounds, improving their synthesis was aimed. Novel catalytic ways were developped for the synthesis of triarylimidazoles and spiro[chroméne-oxindoles] with good yields via multicomponent reactions in the presence of citric acid and BF3.OEt2 as catalysts respectively. Two articles demonstarting the catalytic effects of the former catalysts in the synthesis of benzimidazoles and spirooxindole derivatives were published. Original work carried out in Strasbourg has demonstrated the cytotoxic properties of an iminobenzimidazole on cancerous lines. Analogues of this compound, were prepered, to determine the structural requirements for this cytotoxicity. An article describing these works was published inThe Open Medicinal Chemistry Journal. Another cytotoxic agent “fluorizoline” and analogues thereof were also prepared. Two first articles demonstrating the inhibition of KRAS oncogene by this compound and its activity in models of chronic lymphocytic leukemia were also published.
Diplôme : Doctorat En ligne : ../theses/chimie/CHO7370.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11019 Synthèse de composés hétérocycliques à visée anticancèreuse [texte imprimé] / Nora Chouha, Auteur ; Taoues Boumoud, Auteur ; Laurent Desaubry, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 155 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Triarylimidazoles spirooxindoles acide citrique BF3.OEt2 iminobenzimidazole fluorizoline oncogène KRAS cytotoxicité leucémie citric acid KRAS oncogene cytotoxicity leukemia Index. décimale : 540 Chimie et sciences connexes Résumé : Our work focuses on the development of new synthetic approaches to anticancer compounds. This work was carried out at the University of Constantine and Strasbourg, under Prof. Taoues Boumoud and Dr. Laurent Désaubry’s supervision. Triarylimidazoles and spirooxindoles are heterocycles found among several important anti-cancer agents. In order to facilitate the pharmacochemical exploration of these compounds, improving their synthesis was aimed. Novel catalytic ways were developped for the synthesis of triarylimidazoles and spiro[chroméne-oxindoles] with good yields via multicomponent reactions in the presence of citric acid and BF3.OEt2 as catalysts respectively. Two articles demonstarting the catalytic effects of the former catalysts in the synthesis of benzimidazoles and spirooxindole derivatives were published. Original work carried out in Strasbourg has demonstrated the cytotoxic properties of an iminobenzimidazole on cancerous lines. Analogues of this compound, were prepered, to determine the structural requirements for this cytotoxicity. An article describing these works was published inThe Open Medicinal Chemistry Journal. Another cytotoxic agent “fluorizoline” and analogues thereof were also prepared. Two first articles demonstrating the inhibition of KRAS oncogene by this compound and its activity in models of chronic lymphocytic leukemia were also published.
Diplôme : Doctorat En ligne : ../theses/chimie/CHO7370.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11019 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité CHO/7370 CHO/7370 Thèse Bibliothèque principale Thèses Disponible
Titre : Étude phytochimique et biologique de trois espèces Algériennes : Calendula stellata Cav. (Asteraceae), Scabiosa stellata L. (Caprifoliaceae) et Salvia barrelieri Etl. (Lamiaceae). Type de document : texte imprimé Auteurs : Meryem Lehbili, Auteur ; Ahmed Kabouche, Directeur de thèse ; Abdulmagid Alabdul Magid, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 375 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Calendula stellata Scabiosa stellata Salvia barrelieri saponosides iridoïdes triterpenoïdes flavonoïdes activité antioxydante activité antimicrobienne cytotoxicité saponosids iridoids triterpenes flavonoids antioxidant activity antimicrobial activity cytotoxicity الفعالیة البیولوجیة مضادة للأكسدة مضادة للبكتیریا سمیة خلویة Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical study of three species: Calendula stellata, Scabiosa stellata and Salvia barrelieri led to the isolation of 55 compounds, from which 16 were new, by the use of different chromatographic methods of separation and purification on solid or liquid support.
The isolated compounds were distributed through 27 saponosids, 7 triterpenes, 11 flavonoids, 7 iridoids and 2 chlorogenic acid derivatives. Two mono-iridoids and two flavonoids showed an important antiradical activity (IC50:7-16 µM). Fourteen compounds exhibited a good antibacterial activity (MIC: 15- 125 µg/mL). The new compounds showed a low to moderate cytotoxic activity against all tested cell lines, except for the monodesmoside, obtained from Scabiosa stellata (Scabiostellatoside F) which exhibited a good antiproliferative effect against HT1080 (IC50 = 12 µM).
Diplôme : Doctorat En ligne : ../theses/chimie/LEH7306.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10921 Étude phytochimique et biologique de trois espèces Algériennes : Calendula stellata Cav. (Asteraceae), Scabiosa stellata L. (Caprifoliaceae) et Salvia barrelieri Etl. (Lamiaceae). [texte imprimé] / Meryem Lehbili, Auteur ; Ahmed Kabouche, Directeur de thèse ; Abdulmagid Alabdul Magid, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 375 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Calendula stellata Scabiosa stellata Salvia barrelieri saponosides iridoïdes triterpenoïdes flavonoïdes activité antioxydante activité antimicrobienne cytotoxicité saponosids iridoids triterpenes flavonoids antioxidant activity antimicrobial activity cytotoxicity الفعالیة البیولوجیة مضادة للأكسدة مضادة للبكتیریا سمیة خلویة Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical study of three species: Calendula stellata, Scabiosa stellata and Salvia barrelieri led to the isolation of 55 compounds, from which 16 were new, by the use of different chromatographic methods of separation and purification on solid or liquid support.
The isolated compounds were distributed through 27 saponosids, 7 triterpenes, 11 flavonoids, 7 iridoids and 2 chlorogenic acid derivatives. Two mono-iridoids and two flavonoids showed an important antiradical activity (IC50:7-16 µM). Fourteen compounds exhibited a good antibacterial activity (MIC: 15- 125 µg/mL). The new compounds showed a low to moderate cytotoxic activity against all tested cell lines, except for the monodesmoside, obtained from Scabiosa stellata (Scabiostellatoside F) which exhibited a good antiproliferative effect against HT1080 (IC50 = 12 µM).
Diplôme : Doctorat En ligne : ../theses/chimie/LEH7306.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10921 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LEH/7306 LEH/7306 Thèse Bibliothèque principale Thèses Disponible
Titre : Étude phytochimique et évaluation des activités antioxydante, antibactérienne et cytotoxique de deux plantes du genre Phagnalon (Asteraceae) Type de document : texte imprimé Auteurs : Hanene Cherchar ép Saadi, Auteur ; Djemaa Berrehal, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 190 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Asteraceae Phagnalon saxatile Phagnalon sordidum activité antioxydante activité antibactérienne cytotoxicité antioxidant activity antibacterial activity cytotoxicity فعالية مضادة للأكسدة فعالية مضادة للبكتيريا السمية الخلوية Index. décimale : 540 Chimie et sciences connexes Résumé : Our phytochemical and biological works concerned the aerial parts of two species belonging to Asteraeae family : Phagnalon saxatile (L) Cass. and Phagnalon sordidum L.
Several chromatographic methods allowed us to isolate ten products from the n-butanol and ethyl acetate extracts of Phagnalon saxatile from which one is new, and twenty one products from Phagnalon sordidum.
Structures were determined by different spectral methods: nuclear magnetic resonance with different applications (NMR 1H, NMR 13C, COSY, HSQC and HMBC), the mass spectrometry, UV, acid hydrolysis as well as comparison with literature data.
4 methods were used to evaluate the antioxidant activity of Phagnalon sordidum and Phagnalon saxatile. The latter showed a good activity by the use of 4 methods: DPPH, ABTS, CUPRAC and superoxide radical scavenging activity by alkaline DMSO whereas Phagnalon sordidum exhibited a moderate activity with DPPH and CUPRAC tests.
Furthermore, petroleum ether and chloroform extracts from Phagnalon saxatile showed moderate antibacterial activity against the tested strains. The isolated products (1-O- β-D-glucopyranosyl-1,4-dihydroxy-2-((E) 2-oxo-3-butenyl) benzene, 1-O-β-glucopyranosyl- 2(3’,3’-diméthylallyl) hydroquinone and Potassium 4-hydroxy-3-methoxybenzoique acide
methyl ester-5-sulfate) from Phagnalon saxatile showed a moderate cytotoxic activity by the use of MTT method.
Diplôme : Doctorat En ligne : ../theses/chimie/CHE7249.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10841 Étude phytochimique et évaluation des activités antioxydante, antibactérienne et cytotoxique de deux plantes du genre Phagnalon (Asteraceae) [texte imprimé] / Hanene Cherchar ép Saadi, Auteur ; Djemaa Berrehal, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 190 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Asteraceae Phagnalon saxatile Phagnalon sordidum activité antioxydante activité antibactérienne cytotoxicité antioxidant activity antibacterial activity cytotoxicity فعالية مضادة للأكسدة فعالية مضادة للبكتيريا السمية الخلوية Index. décimale : 540 Chimie et sciences connexes Résumé : Our phytochemical and biological works concerned the aerial parts of two species belonging to Asteraeae family : Phagnalon saxatile (L) Cass. and Phagnalon sordidum L.
Several chromatographic methods allowed us to isolate ten products from the n-butanol and ethyl acetate extracts of Phagnalon saxatile from which one is new, and twenty one products from Phagnalon sordidum.
Structures were determined by different spectral methods: nuclear magnetic resonance with different applications (NMR 1H, NMR 13C, COSY, HSQC and HMBC), the mass spectrometry, UV, acid hydrolysis as well as comparison with literature data.
4 methods were used to evaluate the antioxidant activity of Phagnalon sordidum and Phagnalon saxatile. The latter showed a good activity by the use of 4 methods: DPPH, ABTS, CUPRAC and superoxide radical scavenging activity by alkaline DMSO whereas Phagnalon sordidum exhibited a moderate activity with DPPH and CUPRAC tests.
Furthermore, petroleum ether and chloroform extracts from Phagnalon saxatile showed moderate antibacterial activity against the tested strains. The isolated products (1-O- β-D-glucopyranosyl-1,4-dihydroxy-2-((E) 2-oxo-3-butenyl) benzene, 1-O-β-glucopyranosyl- 2(3’,3’-diméthylallyl) hydroquinone and Potassium 4-hydroxy-3-methoxybenzoique acide
methyl ester-5-sulfate) from Phagnalon saxatile showed a moderate cytotoxic activity by the use of MTT method.
Diplôme : Doctorat En ligne : ../theses/chimie/CHE7249.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10841 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité CHE/7249 CHE/7249 Thèse Bibliothèque principale Thèses Disponible Permalink
