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Partager le résultat de cette recherche Interroger des sources externesCaractérisation des souches bactériennes isolées à partir des nodules de quelques légumineuses endémiques de l’Algérie et détermination du polymorphisme génétique / Esma Torche
Titre : Caractérisation des souches bactériennes isolées à partir des nodules de quelques légumineuses endémiques de l’Algérie et détermination du polymorphisme génétique Type de document : texte imprimé Auteurs : Esma Torche, Auteur ; Yacine Benhizia, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 123 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Hedysarum naudinianum Hedysarum perrauderianum Mesorhizobium endémique Enterobacter nodules Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work is carried out in order to study the bacterial symbiot of the root nodules of two wild legume species endemic to Algeria, Hedysarum naudinianum and Hedysarum perrauderianum. The cultivable isolated bacteria underwent a phenotypical characterization including a series of tests. The aptitude of the isolates to form nodules on the roots of the plants hosts is evaluated by the nodulation test. The profiles of the total proteins of the isolates are determined by electrophoresis SDS-PAGE. The molecular characterization is carried out by DNA-based identity analyses; ARDRA profile sorting and 16S rDNA sequencing. The results show that the nodules of the two legume species included enterobactériaceae having 100% 16S rDNA sequence identity with both Enterobacter cloacae and E. ludwigii. In H. perrauderianum, this taxon was the sole cultured isolate while the other legume H. naudinianum seems to lodge a large range of endophytes. While no culturable Rhizobium could be obtained, several attempts were carried out with the nodules of the two leguminous species. A method based on the cloning and the transformation revealed the existence of Mesorhizobium sp in the two species of Hedysarum. The sequence of Mesorhizobium in H. perrauderianum show identities to other mesorhizobia reported in different legumes from mediterranean to Far East locations. Therefore the sequence of the Mesorhizobium within H. naudinianum bears a mutation which is unique and originates a single nucleotide polymorphism (SNP). Data from the microsymbionts appear to suggest interesting clues to interpret the evolutionary ecology of their host plants.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/TOR6669.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9805 Caractérisation des souches bactériennes isolées à partir des nodules de quelques légumineuses endémiques de l’Algérie et détermination du polymorphisme génétique [texte imprimé] / Esma Torche, Auteur ; Yacine Benhizia, Directeur de thèse . - constantine (Route ain el bey, Algérie) : Université Constantine 1, 2014 . - 123 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Hedysarum naudinianum Hedysarum perrauderianum Mesorhizobium endémique Enterobacter nodules Index. décimale : 570 Sciences de la vie. Biologie Résumé : This work is carried out in order to study the bacterial symbiot of the root nodules of two wild legume species endemic to Algeria, Hedysarum naudinianum and Hedysarum perrauderianum. The cultivable isolated bacteria underwent a phenotypical characterization including a series of tests. The aptitude of the isolates to form nodules on the roots of the plants hosts is evaluated by the nodulation test. The profiles of the total proteins of the isolates are determined by electrophoresis SDS-PAGE. The molecular characterization is carried out by DNA-based identity analyses; ARDRA profile sorting and 16S rDNA sequencing. The results show that the nodules of the two legume species included enterobactériaceae having 100% 16S rDNA sequence identity with both Enterobacter cloacae and E. ludwigii. In H. perrauderianum, this taxon was the sole cultured isolate while the other legume H. naudinianum seems to lodge a large range of endophytes. While no culturable Rhizobium could be obtained, several attempts were carried out with the nodules of the two leguminous species. A method based on the cloning and the transformation revealed the existence of Mesorhizobium sp in the two species of Hedysarum. The sequence of Mesorhizobium in H. perrauderianum show identities to other mesorhizobia reported in different legumes from mediterranean to Far East locations. Therefore the sequence of the Mesorhizobium within H. naudinianum bears a mutation which is unique and originates a single nucleotide polymorphism (SNP). Data from the microsymbionts appear to suggest interesting clues to interpret the evolutionary ecology of their host plants.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/TOR6669.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9805 Exemplaires
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Titre : Diversité et structure génétique des populations de Rhizobium leguminosarum symbiovar viciae isolées du pois (Pisum sativum) et de la lentille (Lens culinaris) cultivés dans deux zones éco-climatiques subhumide et semi-aride de l'est Algérien Type de document : texte imprimé Auteurs : Nassira Riah, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Gisèle Laguerre, Directeur de thèse ; Philippe De Lajudie, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 131 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Tags : Pois lentille nodosités Rhizobium diversité symbiose structure génétique des
populations Algérie zones éco-climatiques Pea lentil nodules diversity symbiosis genetic structure of populations Algeria eco-climatic zones ??????? ????? ????? ??????? ?????????? ?????? ???????? ??????? ???????? ??????? ??? ???? ????Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The symbiosis Rhizobium-legume leads to the formation of root nodules fixing atmospheric nitrogen. This natural process has a considerable economic and agronomic interest. In Algeria, the production of food and forage legume is affected by environmental and climatic stresses (high temperature, water deficit...). In this study, total of 237 isolates were isolated from root nodules collected on lentil (Lens culinaris), proteaginous and forage peas (Pisum sativum) grown in the field in six sites representing two contrasting eco-climatic zones, sub humid and semi-arid in Eastern Algeria. The main objective was to determine the
degree of variability of the genetic structure of populations of Rhizobium nodulating among sites and isolated plants in different climatic conditions. Our isolates were characterized by PCR-restriction fragment length polymorphism (RFLP) of the 16S-23S rRNA intergenic region (IGS), and the nodD-F symbiotic region. The combination of these haplotypes allowed to clustered the isolates into 26 distinct genotypes. Phylogenetic analysis of 16S rDNA sequences allowed us to classify all isolates as Rhizobium leguminosarum. Results obtained showed that the variation of symbiotic marker (nodD-F) is low with the predominance of one
haplotype nod g, previously recovered at high frequency in Europe. Sequence analysis of IGS further confirmed its high variability in the studied strains. An AMOVA analysis showed highly significant differentiation in the IGS haplotype distribution between populations from both eco-climatic zones. This difference was reflected by differences in dominant genotype frequencies. Conversely we could not detect any host plant effect. The nodD gene sequence based phylogeny suggests that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in Algeria is low compared to that reported elsewhere in the world.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/RIA6607.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9720 Diversité et structure génétique des populations de Rhizobium leguminosarum symbiovar viciae isolées du pois (Pisum sativum) et de la lentille (Lens culinaris) cultivés dans deux zones éco-climatiques subhumide et semi-aride de l'est Algérien [texte imprimé] / Nassira Riah, Auteur ; Abdelhamid Djekoun, Directeur de thèse ; Gisèle Laguerre, Directeur de thèse ; Philippe De Lajudie, Directeur de thèse . - constantine (Route ain el bey, Algérie) : Université Constantine 1, 2014 . - 131 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Tags : Pois lentille nodosités Rhizobium diversité symbiose structure génétique des
populations Algérie zones éco-climatiques Pea lentil nodules diversity symbiosis genetic structure of populations Algeria eco-climatic zones ??????? ????? ????? ??????? ?????????? ?????? ???????? ??????? ???????? ??????? ??? ???? ????Index. décimale : 570 Sciences de la vie. Biologie Résumé :
The symbiosis Rhizobium-legume leads to the formation of root nodules fixing atmospheric nitrogen. This natural process has a considerable economic and agronomic interest. In Algeria, the production of food and forage legume is affected by environmental and climatic stresses (high temperature, water deficit...). In this study, total of 237 isolates were isolated from root nodules collected on lentil (Lens culinaris), proteaginous and forage peas (Pisum sativum) grown in the field in six sites representing two contrasting eco-climatic zones, sub humid and semi-arid in Eastern Algeria. The main objective was to determine the
degree of variability of the genetic structure of populations of Rhizobium nodulating among sites and isolated plants in different climatic conditions. Our isolates were characterized by PCR-restriction fragment length polymorphism (RFLP) of the 16S-23S rRNA intergenic region (IGS), and the nodD-F symbiotic region. The combination of these haplotypes allowed to clustered the isolates into 26 distinct genotypes. Phylogenetic analysis of 16S rDNA sequences allowed us to classify all isolates as Rhizobium leguminosarum. Results obtained showed that the variation of symbiotic marker (nodD-F) is low with the predominance of one
haplotype nod g, previously recovered at high frequency in Europe. Sequence analysis of IGS further confirmed its high variability in the studied strains. An AMOVA analysis showed highly significant differentiation in the IGS haplotype distribution between populations from both eco-climatic zones. This difference was reflected by differences in dominant genotype frequencies. Conversely we could not detect any host plant effect. The nodD gene sequence based phylogeny suggests that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in Algeria is low compared to that reported elsewhere in the world.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/RIA6607.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9720 Exemplaires
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Titre : أثر التداخل بين K+ / Na+ على تطور و نمو العقد الجذرية لنبات البازلاء Pisum sativum L.)) صنف merveille de kelvedon النامي تحت ظروف ملحية اثناء مرحلة نمو الشتلة Type de document : texte imprimé Auteurs : هاجر سعيد, Auteur ; سعيدة شوقي, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 79 ورقة. Format : 30 سم Note générale : 2نسخ موجودة مكتبة المركزية
Langues : Arabe (ara) Tags : Salinité potassiume Pisum sativum L. Nodules Salinity potassium ???????? ?????????? (Pisum sativum L.)????????
????? ???????Index. décimale : 570 بيولوجيا Résumé : The objective of the research is to evaluate the interactive effects of salinity and potassium on some physiomorphologique traits. The experiment was conducted during the development of pea seedlings (Pisum sativum L.)
var: merveille de kelvedon, the test was conducted in a randomized complete block (split plot) with three levels of NaCl (S0: 0, S1: 50, S2: 150) mMol, and two levels of potassium salt : potassium nitrate (KNO3) (N1: 20, N2: 40) mMol, and potassium acetate (CH3COOK) (A1: 20, A2: 40) mMol with four replicates grown in pots under controlled conditions, the work was executed on 48 expérimentales units.
The results are shown that treatment salts decreased significantly pigment chlorophyll (chla, Chlb, Chla+b), leaf area (Sf), the number of nodules (Nd), root length and stem (Lt, Lr) and the dry weight of shoots and roots (PSf, PSr) with increasing levels of salinity, the content of potassium (K+) and the selectivity coefficient (K+ / Na+) of leaves and roots, and the content of total nitrogen (NT) in the leaves is reduced with a high salinity concentration compared to the control , whereas the content of proline (Pro) and glucose (Glu) in the leaves and the content of sodium (Na+) in the leaves and roots have been accumulated
considerably when salinity levels are increased.
The application of the two potassium salts in the soil potassium nitrate (KNO3) or potassium acetate (CH3COOK)) is removed deleterious effects of salinity. In comparing these two potassium salts we result, the level of potassium acétate (CH3COOK) (A2) increased the number of nodules (Nd), total nitrogen (NT) in the leaves and the potassium content (K+) in the shoots and roots , but potassium nitrate (KNO3) (N2) decreased the number of nodules (Nd)
compared to the other treatment one, we can conclude that the potassium acétate (CH3COOK) is a good fertilizer than potassium nitrate (KNO3) (CH3COOK) in saline conditions.Diplôme : Magistère En ligne : ../theses/biologie/ASAI4019.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9748 أثر التداخل بين K+ / Na+ على تطور و نمو العقد الجذرية لنبات البازلاء Pisum sativum L.)) صنف merveille de kelvedon النامي تحت ظروف ملحية اثناء مرحلة نمو الشتلة [texte imprimé] / هاجر سعيد, Auteur ; سعيدة شوقي, Directeur de thèse . - constantine (Route ain el bey, Algérie) : Université Constantine 1, 2014 . - 79 ورقة. ; 30 سم.
2نسخ موجودة مكتبة المركزية
Langues : Arabe (ara)
Tags : Salinité potassiume Pisum sativum L. Nodules Salinity potassium ???????? ?????????? (Pisum sativum L.)????????
????? ???????Index. décimale : 570 بيولوجيا Résumé : The objective of the research is to evaluate the interactive effects of salinity and potassium on some physiomorphologique traits. The experiment was conducted during the development of pea seedlings (Pisum sativum L.)
var: merveille de kelvedon, the test was conducted in a randomized complete block (split plot) with three levels of NaCl (S0: 0, S1: 50, S2: 150) mMol, and two levels of potassium salt : potassium nitrate (KNO3) (N1: 20, N2: 40) mMol, and potassium acetate (CH3COOK) (A1: 20, A2: 40) mMol with four replicates grown in pots under controlled conditions, the work was executed on 48 expérimentales units.
The results are shown that treatment salts decreased significantly pigment chlorophyll (chla, Chlb, Chla+b), leaf area (Sf), the number of nodules (Nd), root length and stem (Lt, Lr) and the dry weight of shoots and roots (PSf, PSr) with increasing levels of salinity, the content of potassium (K+) and the selectivity coefficient (K+ / Na+) of leaves and roots, and the content of total nitrogen (NT) in the leaves is reduced with a high salinity concentration compared to the control , whereas the content of proline (Pro) and glucose (Glu) in the leaves and the content of sodium (Na+) in the leaves and roots have been accumulated
considerably when salinity levels are increased.
The application of the two potassium salts in the soil potassium nitrate (KNO3) or potassium acetate (CH3COOK)) is removed deleterious effects of salinity. In comparing these two potassium salts we result, the level of potassium acétate (CH3COOK) (A2) increased the number of nodules (Nd), total nitrogen (NT) in the leaves and the potassium content (K+) in the shoots and roots , but potassium nitrate (KNO3) (N2) decreased the number of nodules (Nd)
compared to the other treatment one, we can conclude that the potassium acétate (CH3COOK) is a good fertilizer than potassium nitrate (KNO3) (CH3COOK) in saline conditions.Diplôme : Magistère En ligne : ../theses/biologie/ASAI4019.pdf Format de la ressource électronique : Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=9748 Exemplaires
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Titre : Isolement et caractérisation des bactéries autochtones, nodulant les légumineuses, piégées à partir de l'habitat naturel de la légumineuse Pisum sativum L. Type de document : texte imprimé Auteurs : Université Mentouri, Editeur scientifique Année de publication : 2010 Importance : 103 f. Note générale : 01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD Langues : Français (fre) Tags : Symbiose Rhizobium léguminosarum bv Pisum sativum Piégeage Nodules Rhizophère Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/GAC5731.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1833 Isolement et caractérisation des bactéries autochtones, nodulant les légumineuses, piégées à partir de l'habitat naturel de la légumineuse Pisum sativum L. [texte imprimé] / Université Mentouri, Editeur scientifique . - 2010 . - 103 f.
01 Disponible à la salle de recherche 01 Disponible au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Tags : Symbiose Rhizobium léguminosarum bv Pisum sativum Piégeage Nodules Rhizophère Index. décimale : 570 Sciences de la vie. Biologie En ligne : ../theses/biologie/GAC5731.pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1833 Exemplaires
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