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Partager le résultat de cette recherche Interroger des sources externesL’effet protecteur des extraits des plantes Helianthemum confertum et Helianthemum ruficomum vis-à-vis de la toxicité induite par la Doxorubicine / Radja Djebbari
Titre : L’effet protecteur des extraits des plantes Helianthemum confertum et Helianthemum ruficomum vis-à-vis de la toxicité induite par la Doxorubicine : Etude in vitro et in vivo Type de document : texte imprimé Auteurs : Radja Djebbari, Auteur ; Djamila Zama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 122 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles.
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Helianthemum confertum Helianthemum ruficomum stress oxydative activités anti oxydants Doxorubicine extraits des plantes peroxydation lipidique composes phénoliques oxidative stress Doxorubicin Antioxidant activities lipid peroxidation Plant extract Phenolic compounds Doxorubicineالمستخلص النباتي الفينولات النشاط المضاد للأكسدة التوترالتاكسدي الأكسدة الفوقية للدهون Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aims to investigate the protective and antioxidant effects of n-butanol extracts of H. confertum and H. ruficomum plants in experimental rats. The n-butanol extract of those plants was evaluated in Wistar Albinos rats for its protective potentials against doxorubicin induced acute toxicity. The effect of n-butanol extracts of H. confertum and H. ruficomum at a dose of (50mg/kg and 100mg/kg) was studied on DOX-induced hepatic, kidney and heart damage. One dose of doxorubicin (10mg/kg and 15mg/kg) was injected intraperitoneal in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, LDH, urea, creatinine, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver, kidney and heart damage. It was found that combined H.
confertum and H. ruficomum (50 and 100mg/kg) extract and DOX (10 and15mg/kg) treatment decreased significantly TBARS level and biochemical parameters. In contract increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the DOX-group. Results of in vivo experiments showed that the n-butanol extracts of H. confertum and H. ruficomum inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity
and maintained the levels of antioxidants enzymes. The protective effect of vitamin E was also studied against doxorubicin-induced acute toxicity at (15mg/kg), and it was found that combined vitamin E (100mg/kg) and DOX (15mg/kg) treatment decreased significantly TBARS level and increased antioxidants activities.
Also, histological study was determined in pretreatment with n-butanol extract of H. confertum and doxorubicin induced acute toxicity at (10mg/kg), and it was found that combined H. confertum n-butanol extract at (50 and 100mg/kg) decreased necrotic cells in hepatic tissue. The antioxidant in vitro tests (DPPH scavenging, inhibition of LPO and anti-cancer activity) of n-butanol extracts of H. confertum and H. ruficomum indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols.Diplôme : Doctorat En ligne : ../theses/biologie/DJE7167.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10590 L’effet protecteur des extraits des plantes Helianthemum confertum et Helianthemum ruficomum vis-à-vis de la toxicité induite par la Doxorubicine : Etude in vitro et in vivo [texte imprimé] / Radja Djebbari, Auteur ; Djamila Zama, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 122 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Helianthemum confertum Helianthemum ruficomum stress oxydative activités anti oxydants Doxorubicine extraits des plantes peroxydation lipidique composes phénoliques oxidative stress Doxorubicin Antioxidant activities lipid peroxidation Plant extract Phenolic compounds Doxorubicineالمستخلص النباتي الفينولات النشاط المضاد للأكسدة التوترالتاكسدي الأكسدة الفوقية للدهون Index. décimale : 570 Sciences de la vie. Biologie Résumé : The present study aims to investigate the protective and antioxidant effects of n-butanol extracts of H. confertum and H. ruficomum plants in experimental rats. The n-butanol extract of those plants was evaluated in Wistar Albinos rats for its protective potentials against doxorubicin induced acute toxicity. The effect of n-butanol extracts of H. confertum and H. ruficomum at a dose of (50mg/kg and 100mg/kg) was studied on DOX-induced hepatic, kidney and heart damage. One dose of doxorubicin (10mg/kg and 15mg/kg) was injected intraperitoneal in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, LDH, urea, creatinine, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver, kidney and heart damage. It was found that combined H.
confertum and H. ruficomum (50 and 100mg/kg) extract and DOX (10 and15mg/kg) treatment decreased significantly TBARS level and biochemical parameters. In contract increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the DOX-group. Results of in vivo experiments showed that the n-butanol extracts of H. confertum and H. ruficomum inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity
and maintained the levels of antioxidants enzymes. The protective effect of vitamin E was also studied against doxorubicin-induced acute toxicity at (15mg/kg), and it was found that combined vitamin E (100mg/kg) and DOX (15mg/kg) treatment decreased significantly TBARS level and increased antioxidants activities.
Also, histological study was determined in pretreatment with n-butanol extract of H. confertum and doxorubicin induced acute toxicity at (10mg/kg), and it was found that combined H. confertum n-butanol extract at (50 and 100mg/kg) decreased necrotic cells in hepatic tissue. The antioxidant in vitro tests (DPPH scavenging, inhibition of LPO and anti-cancer activity) of n-butanol extracts of H. confertum and H. ruficomum indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols.Diplôme : Doctorat En ligne : ../theses/biologie/DJE7167.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10590 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité DJE/7167 DJE/7167 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo Type de document : texte imprimé Auteurs : Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 140 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds التوتر التأكسدي الفعل المضاد للأكسدة المستخلص النباتي المركبات الفينولية الأكسدة الفوقية للدهونIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10833 L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo [texte imprimé] / Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 140 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds التوتر التأكسدي الفعل المضاد للأكسدة المستخلص النباتي المركبات الفينولية الأكسدة الفوقية للدهونIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10833 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAH/7238 LAH/7238 Thèse Bibliothèque principale Thèses Disponible
Titre : دور المستخلص البيتانولي لنباتين من جنس) (Genista et Limoniumفي الوقاية من سمية مبيد الكلوربيريفوس Type de document : texte imprimé Auteurs : عائشة لطرش, Auteur ; جميلة زعمة, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 141 ورقة. Format : 30 سم. Note générale : 2نسخ موجودة مكتبة المركزية
Langues : Arabe (ara) Catégories : Arabe
5 البيولوجياTags : Chlorpyrifos Oxidative stress, Lipid peroxidation Plant extract Flavonoids,AchE Limonium pruinosum Genista ulicina Flavonoids مبيد الكلوربيريفوس التوتر التأكسدي الأكسدة اللبيدية المستخلصات النباتية الفلافونويدات الاستيل كولين استراز Index. décimale : 570 بيولوجيا Résumé : Although mostly known as an organophosphorus pesticides (OPs) Chlorpyrifos (CPF) can react also as a pro-oxidant resulting in the generation of (ROS) (Reactive oxygen species).
The purpose of this study was to evaluate the protective effect of Genista ulicina Spach and Limonium pruinosum extract Towards the oxidative stress which implied (involved) in the toxicity of this pesticide.
Our result indicated that administration of CPF at a dose (10mg/kg/BW) for 10 days by oral gavage induced a significant increase in MDA level in all tissues, organs; transaminase enzymes ALAT, ASAT; Triglycerides,cholesterol, Creatinine and Uric Acid; on the other hand the activity of antioxidant enzyme including GPx, CAT and GSH levels were significantly decreased. Histopathological observations also correlated with the biochemical parameters was shown.
Also, the protective effects of Buthanolic extract of Genista ulicina Spach and Limonium pruinosum (150mg/kg/BW) alone or in combination with CPF were investigated. Co-administration of plants extract with CPF or alone in male rats decreased MDA levels, normalized CAT and GPx activity as well as GSH content and restore some biochemical parameters by suppressing CPF
stress. Furthermore Milder pathological alterations were observed. Overall, the protective effects of plants extract on CPF induced toxicity may be due to its potent antioxidative properties Antioxidant activities of the extracts were studied in vitro. The inhibition
of the formation of malondialdehyde (MDA) and the scavenging of DPPH radicals were assayed. The total phenolic and flavonoid contents of the extracts were determined. The experimental results show that the extracts had antioxidant activity in vitro. The extracts showed a high antioxidant effect, especially scavenging of DPPH anions and inhibition of lipid peroxidation.
That extracts can inhibit membrane LPO and free radical formation due to its free radical scavenging ability. The biological and antioxidant effects exhibited by these extracts could be related to an overall effect of the phenolic compounds present in this extract .Leading to ensure a protective role in attenuating the oxidative stress generated by CPF.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ALAT4337.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10943 دور المستخلص البيتانولي لنباتين من جنس) (Genista et Limoniumفي الوقاية من سمية مبيد الكلوربيريفوس [texte imprimé] / عائشة لطرش, Auteur ; جميلة زعمة, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 141 ورقة. ; 30 سم.
2نسخ موجودة مكتبة المركزية
Langues : Arabe (ara)
Catégories : Arabe
5 البيولوجياTags : Chlorpyrifos Oxidative stress, Lipid peroxidation Plant extract Flavonoids,AchE Limonium pruinosum Genista ulicina Flavonoids مبيد الكلوربيريفوس التوتر التأكسدي الأكسدة اللبيدية المستخلصات النباتية الفلافونويدات الاستيل كولين استراز Index. décimale : 570 بيولوجيا Résumé : Although mostly known as an organophosphorus pesticides (OPs) Chlorpyrifos (CPF) can react also as a pro-oxidant resulting in the generation of (ROS) (Reactive oxygen species).
The purpose of this study was to evaluate the protective effect of Genista ulicina Spach and Limonium pruinosum extract Towards the oxidative stress which implied (involved) in the toxicity of this pesticide.
Our result indicated that administration of CPF at a dose (10mg/kg/BW) for 10 days by oral gavage induced a significant increase in MDA level in all tissues, organs; transaminase enzymes ALAT, ASAT; Triglycerides,cholesterol, Creatinine and Uric Acid; on the other hand the activity of antioxidant enzyme including GPx, CAT and GSH levels were significantly decreased. Histopathological observations also correlated with the biochemical parameters was shown.
Also, the protective effects of Buthanolic extract of Genista ulicina Spach and Limonium pruinosum (150mg/kg/BW) alone or in combination with CPF were investigated. Co-administration of plants extract with CPF or alone in male rats decreased MDA levels, normalized CAT and GPx activity as well as GSH content and restore some biochemical parameters by suppressing CPF
stress. Furthermore Milder pathological alterations were observed. Overall, the protective effects of plants extract on CPF induced toxicity may be due to its potent antioxidative properties Antioxidant activities of the extracts were studied in vitro. The inhibition
of the formation of malondialdehyde (MDA) and the scavenging of DPPH radicals were assayed. The total phenolic and flavonoid contents of the extracts were determined. The experimental results show that the extracts had antioxidant activity in vitro. The extracts showed a high antioxidant effect, especially scavenging of DPPH anions and inhibition of lipid peroxidation.
That extracts can inhibit membrane LPO and free radical formation due to its free radical scavenging ability. The biological and antioxidant effects exhibited by these extracts could be related to an overall effect of the phenolic compounds present in this extract .Leading to ensure a protective role in attenuating the oxidative stress generated by CPF.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/ALAT4337.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10943 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité لطر/4337 لطر/4337 Thèse Bibliothèque principale Thèses Disponible 
Titre : دراسة العلاقة ما بين داء الســــــكري التجــــريبي، سمية الإيثانول و المستخلص البيتانولي لنبات Genista quadriflora : دراسة داخــل و خــــارج العضوية. Type de document : texte imprimé Auteurs : نسيمة بوبكري, Auteur ; جميلة زعمة, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2014 Importance : 160 ورقة. Format : 30 سم Note générale : 2نسخ موجودة مكتبة المركزية
Langues : Arabe (ara) Catégories : Arabe
5 البيولوجياTags : Genista quadriflora Diabetes Streptozotocin Antioxidant activities lipid peroxidation ethanol Anti-inflammatory Plant extract Phenolic compounds. داء السكري النشاط المضاد للأكسدة الأكسدة الفوقية للدهون الإيثانول المستخلص النباتي مضاد للإلتهاب الفينولات Index. décimale : 570 بيولوجيا Résumé : The present study aims to investigate the hypoglycemic and antioxidant effect of butanol extract of Genista quadriflora plant in experimental diabetic rats. The n-butanol extract of this plant was evaluated in Wistar Albinos rats for anti-inflammatory activity using carrageenan-induced hind paw edema model. Diabetes was induced by streptozotocin (55mg/kg, IV), the butanol extract (200 mg / kg) is administered orally for 6 days before induction of diabetes and 18 days after, the glibenclamide was administed orally at the dose (5 mg / kg) for 18 days. Diabetes was associated with significant increase of: glucose, cholesterol, triglycerides, creatinine, MDA in liver, kidney and pancreas, and decrease in antioxidant enzyme defense system, and induce increased serum transminases. n-butanol extract of Genista quadriflora and glibenclamide significantly decreased the levels of AST, ALT, TBARS, glucose, cholesterol, triglycerides, creatinine and also GSH, significantly elevated the activities of CAT and GPx in liver, kidney and pancreas. Histopathological observations also correlated with the biochemical parameters. Thus, the data indicate that treatment with buthanol extracts of Genista quadriflora offers protection against free radical-mediated oxidative stress in organs of animals with experimental diabetes. Oral treatment with n- butanol extract of Genista quadriflora elicited an inhibitory activity on the development of the paw edema induced by carrageen. This extract showed a significant reduction in the paw edema volume in a dose-dependent.
Excessive ethanol intake induces severe tissue damage particularly in the liver through the generation of reactive oxygen species. This study was designed to examine the effect of n-butanol extract of Genista quadriflora for its protective potentials against ethanol induced toxicity. The effect of n-butanol extract of Genista quadriflora at a dose of 200 mg/kg and 100 mg/kg was studied on ethanol induced hepatic damage (40% v/v, 3 g/kg per oral every 12 hours for 3 doses) in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver and kidney damage. A histological study was determined.
It was found that combined Genista quadriflora (200mg/kg) extract and ethanol treatment decreased significantly TBARS level and increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the ethanol group. Results of in vivo experiments showed that the n-butanol extract of Genista quadriflora inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity and maintained the levels of antioxidants enzymes.
The antioxidant tested In vitro (DPPH scavenging .OH and inhibition of LPO) of extracts of different phases (Phase ethyl acetate; butanol phase) of Genista quadriflora plant extract it indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols (Levels flavonoids and total phenols are relatively high).
البيولوجياDiplôme : Doctorat en sciences En ligne : ../theses/biologie/ABOU4028.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9785 دراسة العلاقة ما بين داء الســــــكري التجــــريبي، سمية الإيثانول و المستخلص البيتانولي لنبات Genista quadriflora : دراسة داخــل و خــــارج العضوية. [texte imprimé] / نسيمة بوبكري, Auteur ; جميلة زعمة, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2014 . - 160 ورقة. ; 30 سم.
2نسخ موجودة مكتبة المركزية
Langues : Arabe (ara)
Catégories : Arabe
5 البيولوجياTags : Genista quadriflora Diabetes Streptozotocin Antioxidant activities lipid peroxidation ethanol Anti-inflammatory Plant extract Phenolic compounds. داء السكري النشاط المضاد للأكسدة الأكسدة الفوقية للدهون الإيثانول المستخلص النباتي مضاد للإلتهاب الفينولات Index. décimale : 570 بيولوجيا Résumé : The present study aims to investigate the hypoglycemic and antioxidant effect of butanol extract of Genista quadriflora plant in experimental diabetic rats. The n-butanol extract of this plant was evaluated in Wistar Albinos rats for anti-inflammatory activity using carrageenan-induced hind paw edema model. Diabetes was induced by streptozotocin (55mg/kg, IV), the butanol extract (200 mg / kg) is administered orally for 6 days before induction of diabetes and 18 days after, the glibenclamide was administed orally at the dose (5 mg / kg) for 18 days. Diabetes was associated with significant increase of: glucose, cholesterol, triglycerides, creatinine, MDA in liver, kidney and pancreas, and decrease in antioxidant enzyme defense system, and induce increased serum transminases. n-butanol extract of Genista quadriflora and glibenclamide significantly decreased the levels of AST, ALT, TBARS, glucose, cholesterol, triglycerides, creatinine and also GSH, significantly elevated the activities of CAT and GPx in liver, kidney and pancreas. Histopathological observations also correlated with the biochemical parameters. Thus, the data indicate that treatment with buthanol extracts of Genista quadriflora offers protection against free radical-mediated oxidative stress in organs of animals with experimental diabetes. Oral treatment with n- butanol extract of Genista quadriflora elicited an inhibitory activity on the development of the paw edema induced by carrageen. This extract showed a significant reduction in the paw edema volume in a dose-dependent.
Excessive ethanol intake induces severe tissue damage particularly in the liver through the generation of reactive oxygen species. This study was designed to examine the effect of n-butanol extract of Genista quadriflora for its protective potentials against ethanol induced toxicity. The effect of n-butanol extract of Genista quadriflora at a dose of 200 mg/kg and 100 mg/kg was studied on ethanol induced hepatic damage (40% v/v, 3 g/kg per oral every 12 hours for 3 doses) in Wistar Albinos rats. Serum transaminases, cholesterol, triglycerides, lipid peroxidation (MDA), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase were estimated to access liver and kidney damage. A histological study was determined.
It was found that combined Genista quadriflora (200mg/kg) extract and ethanol treatment decreased significantly TBARS level and increased reduced glutathione, glutathione peroxidase, and catalase activities compared to the ethanol group. Results of in vivo experiments showed that the n-butanol extract of Genista quadriflora inhibited lipid peroxidation, protected the experimental animals from hepatic and kidney toxicity and maintained the levels of antioxidants enzymes.
The antioxidant tested In vitro (DPPH scavenging .OH and inhibition of LPO) of extracts of different phases (Phase ethyl acetate; butanol phase) of Genista quadriflora plant extract it indicated that different function as free radical scavengers, such activities are more strongly correlated with the levels of flavonoids and total phenols (Levels flavonoids and total phenols are relatively high).
البيولوجياDiplôme : Doctorat en sciences En ligne : ../theses/biologie/ABOU4028.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9785 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité بوب/4028 بوب/4028 Thèse Bibliothèque principale Thèses Disponible Documents numériques
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