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Partager le résultat de cette recherche Interroger des sources externesUtilisation des techniques d'électrophorèse pour l'identification et l'étude de la diversité de Rhizobiums. / Borhane Samir Grama
Titre : Utilisation des techniques d'électrophorèse pour l'identification et l'étude de la diversité de Rhizobiums. Type de document : texte imprimé Auteurs : Borhane Samir Grama, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2008 Importance : 68 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Electrophorèse Protéine Enzyme Diversité Rhizobia protein enzyme electrophoresis diversity Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study related to 7 bacterial strains isolated from the nodules of Hedysarum coronarium, 7 strains of Trifolium campestre, 9 strains of Vicia Sativa L and 10 strains of Vicia faba major. References strains of rhizobiums such as R. Sullae, R leguminosarum bv trifoli, R. leguminosarum bv viceae were used.
After morphological and cultural test, the appreciation of the diversity of Rhizobia was appreciated by monodimensional electrophoresis on freezing polyacrylamide in the presence of a detergent (SDS-PAGE) allowing the fractionation of total proteins according to Laemmli (1970) and also by the technique of electrophoresis multilocus of enzymes (MLEE) of some enzymatic systems (ADH, ALD, GOT) according to (Selander et al., 1986).
The result shows diversity in the populations local and also compared to the references
strains.Diplôme : Magistère En ligne : ../theses/biologie/GRA5127.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1761 Utilisation des techniques d'électrophorèse pour l'identification et l'étude de la diversité de Rhizobiums. [texte imprimé] / Borhane Samir Grama, Auteur ; Douadi Khelifi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2008 . - 68 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Electrophorèse Protéine Enzyme Diversité Rhizobia protein enzyme electrophoresis diversity Index. décimale : 570 Sciences de la vie. Biologie Résumé : This study related to 7 bacterial strains isolated from the nodules of Hedysarum coronarium, 7 strains of Trifolium campestre, 9 strains of Vicia Sativa L and 10 strains of Vicia faba major. References strains of rhizobiums such as R. Sullae, R leguminosarum bv trifoli, R. leguminosarum bv viceae were used.
After morphological and cultural test, the appreciation of the diversity of Rhizobia was appreciated by monodimensional electrophoresis on freezing polyacrylamide in the presence of a detergent (SDS-PAGE) allowing the fractionation of total proteins according to Laemmli (1970) and also by the technique of electrophoresis multilocus of enzymes (MLEE) of some enzymatic systems (ADH, ALD, GOT) according to (Selander et al., 1986).
The result shows diversity in the populations local and also compared to the references
strains.Diplôme : Magistère En ligne : ../theses/biologie/GRA5127.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=1761 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité GRA/5127 GRA/5127 Thèse Bibliothèque principale Thèses Disponible 
Titre : Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique Type de document : texte imprimé Auteurs : Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 211 f. Format : 30 cm Note générale : Doctorat 3ème cycle
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis القمح؛التنوع؛ بروتينات؛الهجرة الكهربائية للبروتينات؛حساسية للقمح؛المضادات الجسمية للمرضى من نوع ؛"IgE"بروتينات القمح مسببة الحساسية؛التحليل البروتيومي؛ Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9422 Diversité génétique des céréales,recherche et caractérisation de leurs allergénes par l'approche protéomique [texte imprimé] / Ines Bellil, Auteur ; Douadi Khelifi, Directeur de thèse ; Sandra Denery, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 211 f. ; 30 cm.
Doctorat 3ème cycle
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Blé Triticale Diversité Protéines Electrophorèses Allergies au blé Anticorps IgE Allergènes du blé Immunoblotting ELISA Analyse protéomique Wheat Diversity Proteins Electrophoresis Wheat allergy IgE antibodies Wheat allergens Immunoblitting Proteomic analysis القمح؛التنوع؛ بروتينات؛الهجرة الكهربائية للبروتينات؛حساسية للقمح؛المضادات الجسمية للمرضى من نوع ؛"IgE"بروتينات القمح مسببة الحساسية؛التحليل البروتيومي؛ Index. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, two collections of wheat and one collection of triticale cultivated are analyzed to assess the genetic polymorphism of HMW-GS and SG-FPM: a collection of 69 triticale grown in France, another collection of 856 accessions of local durum wheat collected in Algeria and a priori divided into 17 botanical varieties according to their morphological characteristics, and the last two collections of 40 and 30 varieties of bread and durum wheats from Algerian oases. Biochemical analysis was performed by SDS-PAGE. In hexaploid triticale collection, allele nomenclature was compiled with a total of 36 alleles expressed at seven loci Glu-A1, Glu-B1, Glu-R1, Gli-R2, B2-Glu, Glu-A3, Glu- B3. This diversity has highlighted two major groups of triticale: winter and spring triticales.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
Analysis of Saharan wheats has identified a total of 32 and 29alleles for bread and durum wheat, respectively, which in combination resulted in 36 and 29 different patterns respectively. In bread wheat 3, 9 (including 1 new) and 4 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were expressed at Glu-A1 locus and 9 (including 1 new) at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 7, 8 (including 1 new) and 2 alleles were identified. Knowledge of these alleles is an important one hand to estimate the genetic variability and varietal identification and secondly in the selection for grain quality.
Another part of this study aimed to characterize the major allergens of wheat and / or cereals, to break down the specific IgE response to evaluate the role of different protein families in the allergenicity of wheat. The analysis included 10 varieties and 14 sera from allergic Algerian and French patients to wheat. All biochemical and immunological analysis showed that all proteins are recognized by patients regardless of clinical grade observed. 90% of the sera reacted fraction to albumins / globulins fraction in immunoblotting. IgE sera recognize more than one wheat protein and results can be observed that the immunoreactivity varies with genotypes. And the antigenic profile that emerges from these results is composed of five major antigens, albumins / globulins, /, and gliadins and glutenins subunits low molecular weight and a minor antigen subunits of glutenin high molecular weight. It is difficult to conclude on the type of sensitization to one or more common epitopes of these patients. Four protein bands were identified by proteomic analysis. Confronted by mass spectrometry analysis LC-MS/MS, we can suggest the existence of thirteen wheat allergens in the four groups analyzed.
Diplôme : Doctorat En ligne : ../theses/biologie/BEL6405.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9422 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/6405 BEL/6405 Thèse Bibliothèque principale Thèses Disponible Documents numériques
texte intègraleAdobe Acrobat PDF
Titre : Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien Type de document : texte imprimé Auteurs : Samira Malki, Auteur ; A Yahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 186 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Teucrium polium L. Capitatum Lamiaceae trichome électrophorèse CLHP polyphenols activité antioxydante activité antibactérienne effet diurétique trichomes electrophoresis HPLC antioxidant activity antibacterial activity diuretic effect العائلة الشفوية الأوبار الفصل
الكهربائي الوظيفة المضادة للأكسدة الوظيفة المضادة للبكتيريا فعل ادرار البولIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of our work has focused on the study of Teucrium polium L. Capitatum, which is a widespread medicinal plant in algerian steppes. Several study topics were investigated such as the morphology, physio-biochemical constituents and biological effects.
The morphological analysis consisted of ten populations sampled at different habitats in eastern Algeria. This study revealed that the leaves have the lowest number (five out of ten) of types of trichomes, followed by the calyx and corolla with eight types for each. This confirms the absence of intraspecific variability within the study populations. Furthermore, the analysis of relationships between morphological characteristics and environmental factors of their habitat showed that the latter influences the length of trichomes.
The use of one-dimensional electrophoresis technique on polyacrylamide gel in the presence of detergent (SDS-PAGE) allowed to determine the total protein profiles of the ten study populations. The similarity coefficients of polypeptide bands indicated high similarity between populations.
Biochemical analyzes of the three phenotypes of T. polium L. Capitatum showed that extracts of purple phenotypes were richest in anthocyanins, soluble sugars and carotenoids compared with pink and white phenotypes enlightening there by the importance of flower color.
The combination of certain chemical analysis methods, spectrophotometry (UV-visible) and chromatographic (HPLC) have identified three flavonoids including mostly the cyanidin and two other flavonoids, quercetin and caffeic acid.
The evaluation of the antioxidant potency, that was conducted using the method of trapping the free radical DPPH and that of iron reduction FRAP, revealed that the extract of Biskra population showed higher reducing capacity compared to that of Sétif, with a strong trapping of DPPH● radicals.
As regards to the antibacterial activity, it appears that extracts from two populations have acceptable antibacterial properties.
The pharmacological effect of T. polium L. Capitatum showed an interesting diuretic property.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAL6998.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10396 Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien [texte imprimé] / Samira Malki, Auteur ; A Yahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 186 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Teucrium polium L. Capitatum Lamiaceae trichome électrophorèse CLHP polyphenols activité antioxydante activité antibactérienne effet diurétique trichomes electrophoresis HPLC antioxidant activity antibacterial activity diuretic effect العائلة الشفوية الأوبار الفصل
الكهربائي الوظيفة المضادة للأكسدة الوظيفة المضادة للبكتيريا فعل ادرار البولIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of our work has focused on the study of Teucrium polium L. Capitatum, which is a widespread medicinal plant in algerian steppes. Several study topics were investigated such as the morphology, physio-biochemical constituents and biological effects.
The morphological analysis consisted of ten populations sampled at different habitats in eastern Algeria. This study revealed that the leaves have the lowest number (five out of ten) of types of trichomes, followed by the calyx and corolla with eight types for each. This confirms the absence of intraspecific variability within the study populations. Furthermore, the analysis of relationships between morphological characteristics and environmental factors of their habitat showed that the latter influences the length of trichomes.
The use of one-dimensional electrophoresis technique on polyacrylamide gel in the presence of detergent (SDS-PAGE) allowed to determine the total protein profiles of the ten study populations. The similarity coefficients of polypeptide bands indicated high similarity between populations.
Biochemical analyzes of the three phenotypes of T. polium L. Capitatum showed that extracts of purple phenotypes were richest in anthocyanins, soluble sugars and carotenoids compared with pink and white phenotypes enlightening there by the importance of flower color.
The combination of certain chemical analysis methods, spectrophotometry (UV-visible) and chromatographic (HPLC) have identified three flavonoids including mostly the cyanidin and two other flavonoids, quercetin and caffeic acid.
The evaluation of the antioxidant potency, that was conducted using the method of trapping the free radical DPPH and that of iron reduction FRAP, revealed that the extract of Biskra population showed higher reducing capacity compared to that of Sétif, with a strong trapping of DPPH● radicals.
As regards to the antibacterial activity, it appears that extracts from two populations have acceptable antibacterial properties.
The pharmacological effect of T. polium L. Capitatum showed an interesting diuretic property.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAL6998.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10396 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MAL/6998 MAL/6998 Thèse Bibliothèque principale Thèses Disponible
Titre : Variation des sous unités glutenines de haut poids moléculaire chez les espèces aegilops de la séction Sitopsis Type de document : texte imprimé Auteurs : Rima Hind Boudchicha, Auteur ; Univ. de Constantine, Éditeur scientifique ; Douadi Khelifi, Directeur de thèse Mention d'édition : 15 /10/2009 Année de publication : 2009 Importance : 73 f. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD. Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie: biotechnologie végétale Blé Electrophorèse Protéines Diversité Aegilops Sitopsis wheat proteins diversity electrophoresis القمح البروتینات التباین الھجرة الكھربائیة Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study is based on the variation of subunits glutenin high molecular weight HPM of 66 accessions of Aegilops belonging to the section Sitopsis, colled: Ae.bicornis, Ae.longissima, Ae.sharonensis, Ae.seassii and Ae.speltoides, using monodimensionnel electrophoresis. The presence of 3 glutenin bands in several accessions of Ae.longissima seems to be evidence for the existance of at least 3 genes encoding the SG-HPM in the genome S of Aegilops. The technic of SDS-PAGE has permitted us to estimate a high genetic variability of SG-HPM in Ae.speltoides. These results can be used in improving the technological quality of wheat. From the comparison between the glutenin patterns found in the species analyzed and those band observed for the B genome of hexaploid wheat, it can be concluded that Ae.bicornis and Ae.longissima are unlikely donors of the B genome.Note de contenu :
Annexes.Diplôme : Magistère En ligne : ../theses/biologie/BOU5521.pdf Permalink : index.php?lvl=notice_display&id=1802 Variation des sous unités glutenines de haut poids moléculaire chez les espèces aegilops de la séction Sitopsis [texte imprimé] / Rima Hind Boudchicha, Auteur ; Univ. de Constantine, Éditeur scientifique ; Douadi Khelifi, Directeur de thèse . - 15 /10/2009 . - 2009 . - 73 f.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie: biotechnologie végétale Blé Electrophorèse Protéines Diversité Aegilops Sitopsis wheat proteins diversity electrophoresis القمح البروتینات التباین الھجرة الكھربائیة Index. décimale : 570 Sciences de la vie. Biologie Résumé :
This study is based on the variation of subunits glutenin high molecular weight HPM of 66 accessions of Aegilops belonging to the section Sitopsis, colled: Ae.bicornis, Ae.longissima, Ae.sharonensis, Ae.seassii and Ae.speltoides, using monodimensionnel electrophoresis. The presence of 3 glutenin bands in several accessions of Ae.longissima seems to be evidence for the existance of at least 3 genes encoding the SG-HPM in the genome S of Aegilops. The technic of SDS-PAGE has permitted us to estimate a high genetic variability of SG-HPM in Ae.speltoides. These results can be used in improving the technological quality of wheat. From the comparison between the glutenin patterns found in the species analyzed and those band observed for the B genome of hexaploid wheat, it can be concluded that Ae.bicornis and Ae.longissima are unlikely donors of the B genome.Note de contenu :
Annexes.Diplôme : Magistère En ligne : ../theses/biologie/BOU5521.pdf Permalink : index.php?lvl=notice_display&id=1802 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/5521 BOU/5521 Thèse Bibliothèque principale Thèses Disponible
Titre : Evaluation des ressources phytogénétiques des blés cultivés en Algérie et de leurs apparentés. : Analyse de l’expression différentielle de protéines dans le grain selon deux conditions de culture (biologique versus conventionnelle) Type de document : texte imprimé Auteurs : Ouahiba Hamdi, Auteur ; Douadi Khelifi, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2011 Importance : 132 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : collections de blé sous unités gluténines électrophorèse variabilité protome endosperme conditions environmentales culture conventionnelle culture biologique wheat collections Glutenin subunits electrophoresis Variability endosperm environmental conditions conventionel regime organic regime مجموعات من القمح جلوتينين تغير الهجرة الكهربائية للبروتينات بروتيوم النمط الزراعي التقليدي النمط
الزراعي البيولوجيIndex. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, several collections were analyzed in order to appreciate the allelic variation of high and low-molecular-weight (HMW and LMW) glutenin subunits composition: a collection of 59 accessions representing 5 genomes (U, C, M, D, N), collection of 856 accessions of local durum wheat (Triticum durum Desf.) collected in Algeria and divided a priori according to their agronomical and morphological traits in 17 varieties, a collection of thirty accessions of lines sisters coming from the ICARDA, obtained from interspecific crossings between 2 Syrian varieties of durum wheat (Cham and Oum Rabi) and 2 related species (T. dicoccum and T. polonicum) and two other collections of 71 bread wheat and 120 durum wheat germoplasm grown in Algeria. Seed samples were provided for the most part by the International Center for Agricultural Research in the Dry Areas (Alep, Syria). The biochemical analysis was carried out by monodimensionel SDS-PAGE.
In Aegilops collection, several new alleles corresponding to HMW subunits were detected and an allelic nomenclature was drawn up in which a total of 16 alleles were exprimed at the locus Glu-U1, 13 at Glu-M1, 9 at Glu-C1, 6 at Glu-N1 and 10 at the Glu-D1 locus.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1 and were analyzed to determine the number of thiol groups. Three and Seven cysteine residues were found respectively. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
In the collection of related durum wheat, we can count six allelic forms, which two are localised at the locus Glu-A1, it acts of the allele “a” and the allele “c” and four at the locus Glu-B1, they are “a, b, d and i”. While referring to international allelic nomenclature, these alleles code for following proteins: 1, nul, 7, 7+8, 6+8 and 17+18 respectively. Important polymorphism caracterise HMW-GS with a total of 13 alleles identified.The knowledge of these alleles constitutes an important tool for the identification varietal of wheat.
Extensive variation has been found in bread and durum wheat germoplasm grown in Algeria. In bread wheat 3, 6 and 5 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were exprimeted at Glu-A1 locus and 9 at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 5,8 and 2 alleles were identified.
The new HMW and LMW glutenin variation found in this work suggests their possible utilisation in breeding for wheat quality. The aim of the second part of this study was to evaluate the differences due to the culture regimes (biological versus conventional) in composition of total and metabolic proteins in the mature wheat grain. The analyses were carried out on three classes of sizes of grains (small, middle and large) from two varieties of bread wheat: Ataro and Renan. The two varieties exhibited differences on the grain sizes distribution. The one-dimensional electrophoretic analyses of storage and metabolic proteins showed a significant effect of the culture regime on HMW-SG and LMW-SG for the two varieties. Many gliadins (5 bands out of 16 for Renan and 11 out of 22 for Ataro) were influenced by culture regimes. The proteomic approach, carried out on the Ataro variety, revealed for all sizes of grains, 20 variable spots of which three were more expressed in biological regime. Analysis of the proteome between sizes revealed 149 spots quantitatively different from one class to another for the biological regime, against 115 for the conventional regime. In addition to storage proteins, spectrometry analysis using MALDI-TOF and the database interrogations, allowed serpines and proteins involved in the sugars metabolism to be identified.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM6350.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=6320 Evaluation des ressources phytogénétiques des blés cultivés en Algérie et de leurs apparentés. : Analyse de l’expression différentielle de protéines dans le grain selon deux conditions de culture (biologique versus conventionnelle) [texte imprimé] / Ouahiba Hamdi, Auteur ; Douadi Khelifi, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2011 . - 132 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : collections de blé sous unités gluténines électrophorèse variabilité protome endosperme conditions environmentales culture conventionnelle culture biologique wheat collections Glutenin subunits electrophoresis Variability endosperm environmental conditions conventionel regime organic regime مجموعات من القمح جلوتينين تغير الهجرة الكهربائية للبروتينات بروتيوم النمط الزراعي التقليدي النمط
الزراعي البيولوجيIndex. décimale : 570 Sciences de la vie. Biologie Résumé : In the first part of this study, several collections were analyzed in order to appreciate the allelic variation of high and low-molecular-weight (HMW and LMW) glutenin subunits composition: a collection of 59 accessions representing 5 genomes (U, C, M, D, N), collection of 856 accessions of local durum wheat (Triticum durum Desf.) collected in Algeria and divided a priori according to their agronomical and morphological traits in 17 varieties, a collection of thirty accessions of lines sisters coming from the ICARDA, obtained from interspecific crossings between 2 Syrian varieties of durum wheat (Cham and Oum Rabi) and 2 related species (T. dicoccum and T. polonicum) and two other collections of 71 bread wheat and 120 durum wheat germoplasm grown in Algeria. Seed samples were provided for the most part by the International Center for Agricultural Research in the Dry Areas (Alep, Syria). The biochemical analysis was carried out by monodimensionel SDS-PAGE.
In Aegilops collection, several new alleles corresponding to HMW subunits were detected and an allelic nomenclature was drawn up in which a total of 16 alleles were exprimed at the locus Glu-U1, 13 at Glu-M1, 9 at Glu-C1, 6 at Glu-N1 and 10 at the Glu-D1 locus.
Important variation was found in the collection of botanic durum wheat. Among the 16 alleles identified at the Glu-1 loci, two were new. The first named Glu-B1i1 encoding for two bands located between 17 and 18 which was assigned the nomenclature 171 and 181. The other named Glu-B1e1 codes two bands similar to 20x and 20y but with faster mobility, which were named 20x1-20y1 and were analyzed to determine the number of thiol groups. Three and Seven cysteine residues were found respectively. At Glu-3 and Glu-2 loci, 19 alleles were identified, where the allele named Glu-B3ab (encoding for subunits 2-8-9-13-16) was considered as new.
In the collection of related durum wheat, we can count six allelic forms, which two are localised at the locus Glu-A1, it acts of the allele “a” and the allele “c” and four at the locus Glu-B1, they are “a, b, d and i”. While referring to international allelic nomenclature, these alleles code for following proteins: 1, nul, 7, 7+8, 6+8 and 17+18 respectively. Important polymorphism caracterise HMW-GS with a total of 13 alleles identified.The knowledge of these alleles constitutes an important tool for the identification varietal of wheat.
Extensive variation has been found in bread and durum wheat germoplasm grown in Algeria. In bread wheat 3, 6 and 5 alleles were observed at Glu-A1, Glu-B1 and Glu-D1 loci encoding HMW-GS respectively. LMW-GS displayed similar polymorphism, as 4, 9 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-D3 respectively. In durum wheat collection, 3 alleles were exprimeted at Glu-A1 locus and 9 at Glu-B1. At Glu-A3, Glu-B3 and Glu-B2 loci 5,8 and 2 alleles were identified.
The new HMW and LMW glutenin variation found in this work suggests their possible utilisation in breeding for wheat quality. The aim of the second part of this study was to evaluate the differences due to the culture regimes (biological versus conventional) in composition of total and metabolic proteins in the mature wheat grain. The analyses were carried out on three classes of sizes of grains (small, middle and large) from two varieties of bread wheat: Ataro and Renan. The two varieties exhibited differences on the grain sizes distribution. The one-dimensional electrophoretic analyses of storage and metabolic proteins showed a significant effect of the culture regime on HMW-SG and LMW-SG for the two varieties. Many gliadins (5 bands out of 16 for Renan and 11 out of 22 for Ataro) were influenced by culture regimes. The proteomic approach, carried out on the Ataro variety, revealed for all sizes of grains, 20 variable spots of which three were more expressed in biological regime. Analysis of the proteome between sizes revealed 149 spots quantitatively different from one class to another for the biological regime, against 115 for the conventional regime. In addition to storage proteins, spectrometry analysis using MALDI-TOF and the database interrogations, allowed serpines and proteins involved in the sugars metabolism to be identified.Diplôme : Doctorat en sciences En ligne : ../theses/biologie/HAM6350.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=6320 Exemplaires (1)
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