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Partager le résultat de cette recherche Interroger des sources externesEtude chimique et biologique des espèces Calligonum azel et Calligonum comosum (Polygonaceae) et une espèce du genre Thymus (Lamiaceae). / Soumia Belaabed Khalfaoui
Titre : Etude chimique et biologique des espèces Calligonum azel et Calligonum comosum (Polygonaceae) et une espèce du genre Thymus (Lamiaceae). Type de document : texte imprimé Auteurs : Soumia Belaabed Khalfaoui, Auteur ; Nour-eddine Beghidja, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 250 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Calligonum azel Calligonum comosum Thymus ciliatus Polygonaceae Lamiaceae RMN 1D et 2D CLHP HRESIMS activités biologiques huile essentielle NMR 1D and 2D HPLC Biological activities essential oil مطيافية الرنين المغناطيسي1D و2D كروماتوغرافيا HPLC مطيافية الكتلة النضاطات البيولوجية الزيوت الأساسية Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical and biological study concerns the aerial parts of two endemic Saharian species belonging to the polygonaceae family: Calligonum azel and Calligonum comosum and a third one belonging to the Lamiaceae family.
The object of our work focused on the valorization of the Algerian flora. For this, firstly; we carried out a phytochemical study on Calligonum azel and Calligonum comosum which concerned mainly the biologically active compounds then we tested the antioxidant, antibacterial, antifungal and anti-inflammatory properties of all the organic extracts and some isolated compounds. Secondly; we studied the chemical composition and biological activities of Thymus ciliatus essential oil.
The study of aerial parts of C. azel and C. comosum, in order to know the active compounds responsible for their biological activity, leading to purification, chromatographic identification (CC, TLC and HPLC), and characterization using the different spectroscopic methods (1D and 2D NMR, MS and UV) of twenty one pure compounds (21) in which eighteen (18) compounds were identified.
The investigation of the biological activities of the extracts of Calligonum azel and Calligonum comosum showed that the best antioxidant activity was exhibited by the Chloroform and ethyl acetate extracts of C. azel and the Chloroform extract of C. comosum. The chloroform extracts of both species showed the best antibacterial activity. Chloroform extract from C. comosum has better antifungal activity against Candida albicans ATCC 2019 and Candida parapsilosis ATCC 22019.
From the isolated compounds, three have an antioxidant and anti-inflammatory activity. These compounds were: protocatachuic ethyl ester acid, -diamino (4-hydroxy benzene) butanoic acid and tamgernanetin.
The qualitative and quantitative analysis of essential oil of T. ciliatus allows the identification of 25 compounds representing 87.18 % of the total essential oil mainly. The GC and GC-MS analysis showed that this essential oils was mainly rich of thymol (54.26%), carvacrol methyl ether (1,373 %), o-cymene (5.72 %) carvacrol (1.373%)-cymen-7-ol (17.58 %), however it was the first time that -cymen-7-ol (17.58 %) was found as main component in the essential oils of T. ciliatus.
The evaluation of the antibacterial activity was assessed by disc diffusion method against bacterial strains. The essential oil exhibited a good antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 43300 and Staphylocossus aureus.
All of our works confirms the medicinal properties of the Saharian species, and contributes to their scientific descriptions.Diplôme : Doctorat En ligne : ../theses/chimie/BEL7311.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10926 Etude chimique et biologique des espèces Calligonum azel et Calligonum comosum (Polygonaceae) et une espèce du genre Thymus (Lamiaceae). [texte imprimé] / Soumia Belaabed Khalfaoui, Auteur ; Nour-eddine Beghidja, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 250 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Calligonum azel Calligonum comosum Thymus ciliatus Polygonaceae Lamiaceae RMN 1D et 2D CLHP HRESIMS activités biologiques huile essentielle NMR 1D and 2D HPLC Biological activities essential oil مطيافية الرنين المغناطيسي1D و2D كروماتوغرافيا HPLC مطيافية الكتلة النضاطات البيولوجية الزيوت الأساسية Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical and biological study concerns the aerial parts of two endemic Saharian species belonging to the polygonaceae family: Calligonum azel and Calligonum comosum and a third one belonging to the Lamiaceae family.
The object of our work focused on the valorization of the Algerian flora. For this, firstly; we carried out a phytochemical study on Calligonum azel and Calligonum comosum which concerned mainly the biologically active compounds then we tested the antioxidant, antibacterial, antifungal and anti-inflammatory properties of all the organic extracts and some isolated compounds. Secondly; we studied the chemical composition and biological activities of Thymus ciliatus essential oil.
The study of aerial parts of C. azel and C. comosum, in order to know the active compounds responsible for their biological activity, leading to purification, chromatographic identification (CC, TLC and HPLC), and characterization using the different spectroscopic methods (1D and 2D NMR, MS and UV) of twenty one pure compounds (21) in which eighteen (18) compounds were identified.
The investigation of the biological activities of the extracts of Calligonum azel and Calligonum comosum showed that the best antioxidant activity was exhibited by the Chloroform and ethyl acetate extracts of C. azel and the Chloroform extract of C. comosum. The chloroform extracts of both species showed the best antibacterial activity. Chloroform extract from C. comosum has better antifungal activity against Candida albicans ATCC 2019 and Candida parapsilosis ATCC 22019.
From the isolated compounds, three have an antioxidant and anti-inflammatory activity. These compounds were: protocatachuic ethyl ester acid, -diamino (4-hydroxy benzene) butanoic acid and tamgernanetin.
The qualitative and quantitative analysis of essential oil of T. ciliatus allows the identification of 25 compounds representing 87.18 % of the total essential oil mainly. The GC and GC-MS analysis showed that this essential oils was mainly rich of thymol (54.26%), carvacrol methyl ether (1,373 %), o-cymene (5.72 %) carvacrol (1.373%)-cymen-7-ol (17.58 %), however it was the first time that -cymen-7-ol (17.58 %) was found as main component in the essential oils of T. ciliatus.
The evaluation of the antibacterial activity was assessed by disc diffusion method against bacterial strains. The essential oil exhibited a good antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 43300 and Staphylocossus aureus.
All of our works confirms the medicinal properties of the Saharian species, and contributes to their scientific descriptions.Diplôme : Doctorat En ligne : ../theses/chimie/BEL7311.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10926 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEL/7311 BEL/7311 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien Type de document : texte imprimé Auteurs : Samira Malki, Auteur ; A Yahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 186 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Teucrium polium L. Capitatum Lamiaceae trichome électrophorèse CLHP polyphenols activité antioxydante activité antibactérienne effet diurétique trichomes electrophoresis HPLC antioxidant activity antibacterial activity diuretic effect العائلة الشفوية الأوبار الفصل
الكهربائي الوظيفة المضادة للأكسدة الوظيفة المضادة للبكتيريا فعل ادرار البولIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of our work has focused on the study of Teucrium polium L. Capitatum, which is a widespread medicinal plant in algerian steppes. Several study topics were investigated such as the morphology, physio-biochemical constituents and biological effects.
The morphological analysis consisted of ten populations sampled at different habitats in eastern Algeria. This study revealed that the leaves have the lowest number (five out of ten) of types of trichomes, followed by the calyx and corolla with eight types for each. This confirms the absence of intraspecific variability within the study populations. Furthermore, the analysis of relationships between morphological characteristics and environmental factors of their habitat showed that the latter influences the length of trichomes.
The use of one-dimensional electrophoresis technique on polyacrylamide gel in the presence of detergent (SDS-PAGE) allowed to determine the total protein profiles of the ten study populations. The similarity coefficients of polypeptide bands indicated high similarity between populations.
Biochemical analyzes of the three phenotypes of T. polium L. Capitatum showed that extracts of purple phenotypes were richest in anthocyanins, soluble sugars and carotenoids compared with pink and white phenotypes enlightening there by the importance of flower color.
The combination of certain chemical analysis methods, spectrophotometry (UV-visible) and chromatographic (HPLC) have identified three flavonoids including mostly the cyanidin and two other flavonoids, quercetin and caffeic acid.
The evaluation of the antioxidant potency, that was conducted using the method of trapping the free radical DPPH and that of iron reduction FRAP, revealed that the extract of Biskra population showed higher reducing capacity compared to that of Sétif, with a strong trapping of DPPH● radicals.
As regards to the antibacterial activity, it appears that extracts from two populations have acceptable antibacterial properties.
The pharmacological effect of T. polium L. Capitatum showed an interesting diuretic property.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAL6998.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10396 Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien [texte imprimé] / Samira Malki, Auteur ; A Yahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 186 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Teucrium polium L. Capitatum Lamiaceae trichome électrophorèse CLHP polyphenols activité antioxydante activité antibactérienne effet diurétique trichomes electrophoresis HPLC antioxidant activity antibacterial activity diuretic effect العائلة الشفوية الأوبار الفصل
الكهربائي الوظيفة المضادة للأكسدة الوظيفة المضادة للبكتيريا فعل ادرار البولIndex. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of our work has focused on the study of Teucrium polium L. Capitatum, which is a widespread medicinal plant in algerian steppes. Several study topics were investigated such as the morphology, physio-biochemical constituents and biological effects.
The morphological analysis consisted of ten populations sampled at different habitats in eastern Algeria. This study revealed that the leaves have the lowest number (five out of ten) of types of trichomes, followed by the calyx and corolla with eight types for each. This confirms the absence of intraspecific variability within the study populations. Furthermore, the analysis of relationships between morphological characteristics and environmental factors of their habitat showed that the latter influences the length of trichomes.
The use of one-dimensional electrophoresis technique on polyacrylamide gel in the presence of detergent (SDS-PAGE) allowed to determine the total protein profiles of the ten study populations. The similarity coefficients of polypeptide bands indicated high similarity between populations.
Biochemical analyzes of the three phenotypes of T. polium L. Capitatum showed that extracts of purple phenotypes were richest in anthocyanins, soluble sugars and carotenoids compared with pink and white phenotypes enlightening there by the importance of flower color.
The combination of certain chemical analysis methods, spectrophotometry (UV-visible) and chromatographic (HPLC) have identified three flavonoids including mostly the cyanidin and two other flavonoids, quercetin and caffeic acid.
The evaluation of the antioxidant potency, that was conducted using the method of trapping the free radical DPPH and that of iron reduction FRAP, revealed that the extract of Biskra population showed higher reducing capacity compared to that of Sétif, with a strong trapping of DPPH● radicals.
As regards to the antibacterial activity, it appears that extracts from two populations have acceptable antibacterial properties.
The pharmacological effect of T. polium L. Capitatum showed an interesting diuretic property.
Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAL6998.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10396 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité MAL/6998 MAL/6998 Thèse Bibliothèque principale Thèses Disponible
Titre : Isolation, structural determination of secondary metabolites and biological activities of Asphodelus tenuifolius (Liliaceae). Type de document : texte imprimé Auteurs : Ayoub Khalfaoui, Auteur ; Mohamed Bouheroum, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 212 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
ChimieTags : Liliacée Asphodelus tenuifolius anthraquinones polyphénols CLHP RMN 1D et 2D HR-ESI-MS activités biologiques activité cytotoxique potentielantiproliférative Liliaceae polyphenols HPLC NMR 1D 2D HR-ESIMS ECD biological activities cytotoxic activity MTT assay anti-proliferative potential المركبات الفينولية كروماتوغرافيا
HR-ESIMSمطيافية الكتلة الفعالية البيولوجية النشاط السام للخلاياIndex. décimale : 540 Chimie et sciences connexes Résumé :
The works presented in this manuscript is a continuation of the phytochemical research carried out by our phytochemistry group affiliated to the VARENBIOMOL research unit from many years on medicinal and aromatic plants growing in different regions of the country, including the vaste Sahara. The first aim of this research is to value the biological resources related to the plant biodiversity of the Algerian flora and the knowledge associated with this flora in terms of traditional use in medicine, and the second one is the separation and identification of new bioactive compounds with interesting biological activities. The choice of Asphodelus tenuifolius specie for a phytochemical and a biological study in this thesis was based mainly on the fact that this specie belongs to the Liliaceae family known for its richness in various secondary metabolites with a good biological and pharmacogical interest, as well as for the good reputation of using this plant in local traditional medicine. For this purpose, the organic extracts of A. tenuifolius specie were studied chemically using various chromatographic methods (TLC, CC, preparative plates, HPLC…) to lead the isolation and the identification of seventeen pure compounds using various spectroscopic analysis, 1D and 2D NMR (1H, 13C, COSY, HSQC, HMBC ...), ECD analysis, HRESI-MS and ESI-MS spectroscopy, UV-Visible, IR and also with comparison with the literature data. All These data allowed us especially to characterize two new structures of anthraquinones isolated from the Chloroform extract with ten other known compounds dividing into two flavonoids, one sterol, one nitrogen phenolic compound, an anthraquinone and two glycoside anthraquinones with three more compounds in the process of identification, most of these classes are known with their therapeutic benefit and interesting biological activities. Phytochemical study of Ethyl Acetate extract of this specie allowed also the isolation of six known compounds: two phenolic acids, three flavonoids and one anthraquinone. Quantitative estimation of total polyphenols and flavonoids contents (TPC and TFC) showed that the Chloroform extract has a high content of polyphenols and flavonoids compared to Ethyl acetate and Butanol extracts. The biological activities assays like: antioxidant, antibacterial, antifungal, cytotoxic and antiviral performed for the different organic extracts, as well as a cytotoxic activity in A375 (human melanoma) cancer cell line carried out on the two new isolated compounds, confirm the pharmacological and the therapeutic properties of this specie and give an explication of their traditional uses.
Diplôme : Doctorat En ligne : ../theses/chimie/KHA7401.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11051 Isolation, structural determination of secondary metabolites and biological activities of Asphodelus tenuifolius (Liliaceae). [texte imprimé] / Ayoub Khalfaoui, Auteur ; Mohamed Bouheroum, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 212 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
ChimieTags : Liliacée Asphodelus tenuifolius anthraquinones polyphénols CLHP RMN 1D et 2D HR-ESI-MS activités biologiques activité cytotoxique potentielantiproliférative Liliaceae polyphenols HPLC NMR 1D 2D HR-ESIMS ECD biological activities cytotoxic activity MTT assay anti-proliferative potential المركبات الفينولية كروماتوغرافيا
HR-ESIMSمطيافية الكتلة الفعالية البيولوجية النشاط السام للخلاياIndex. décimale : 540 Chimie et sciences connexes Résumé :
The works presented in this manuscript is a continuation of the phytochemical research carried out by our phytochemistry group affiliated to the VARENBIOMOL research unit from many years on medicinal and aromatic plants growing in different regions of the country, including the vaste Sahara. The first aim of this research is to value the biological resources related to the plant biodiversity of the Algerian flora and the knowledge associated with this flora in terms of traditional use in medicine, and the second one is the separation and identification of new bioactive compounds with interesting biological activities. The choice of Asphodelus tenuifolius specie for a phytochemical and a biological study in this thesis was based mainly on the fact that this specie belongs to the Liliaceae family known for its richness in various secondary metabolites with a good biological and pharmacogical interest, as well as for the good reputation of using this plant in local traditional medicine. For this purpose, the organic extracts of A. tenuifolius specie were studied chemically using various chromatographic methods (TLC, CC, preparative plates, HPLC…) to lead the isolation and the identification of seventeen pure compounds using various spectroscopic analysis, 1D and 2D NMR (1H, 13C, COSY, HSQC, HMBC ...), ECD analysis, HRESI-MS and ESI-MS spectroscopy, UV-Visible, IR and also with comparison with the literature data. All These data allowed us especially to characterize two new structures of anthraquinones isolated from the Chloroform extract with ten other known compounds dividing into two flavonoids, one sterol, one nitrogen phenolic compound, an anthraquinone and two glycoside anthraquinones with three more compounds in the process of identification, most of these classes are known with their therapeutic benefit and interesting biological activities. Phytochemical study of Ethyl Acetate extract of this specie allowed also the isolation of six known compounds: two phenolic acids, three flavonoids and one anthraquinone. Quantitative estimation of total polyphenols and flavonoids contents (TPC and TFC) showed that the Chloroform extract has a high content of polyphenols and flavonoids compared to Ethyl acetate and Butanol extracts. The biological activities assays like: antioxidant, antibacterial, antifungal, cytotoxic and antiviral performed for the different organic extracts, as well as a cytotoxic activity in A375 (human melanoma) cancer cell line carried out on the two new isolated compounds, confirm the pharmacological and the therapeutic properties of this specie and give an explication of their traditional uses.
Diplôme : Doctorat En ligne : ../theses/chimie/KHA7401.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11051 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KHA/7401 KHA/7401 Thèse Bibliothèque principale Thèses Disponible
