2 résultat(s) recherche sur le tag 'CLHP'    

Etude chimique et biologique des espèces Calligonum azel et Calligonum comosum (Polygonaceae) et une espèce du genre Thymus (Lamiaceae). / Soumia Belaabed Khalfaoui  

Public ISBD Titre : Etude chimique et biologique des espèces Calligonum azel et Calligonum comosum (Polygonaceae) et une espèce du genre Thymus (Lamiaceae). Type de document : texte imprimé Auteurs : Soumia Belaabed Khalfaoui, Auteur ; Nour-eddine Beghidja, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 250 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais Chimie Tags : Calligonum azel  Calligonum comosum  Thymus ciliatus  Polygonaceae  Lamiaceae  RMN 1D et 2D  CLHP  HRESIMS  activités biologiques  huile essentielle  NMR 1D and 2D  HPLC  Biological activities  essential oil  ??????? ?????? ??????????1D ?2D  ????????????? HPLC  ??????? ??????  ???????? ??????????  ?????? ???????? Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical and biological study concerns the aerial parts of two endemic Saharian species belonging to the polygonaceae family: Calligonum azel and Calligonum comosum and a third one belonging to the Lamiaceae family. The object of our work focused on the valorization of the Algerian flora. For this, firstly; we carried out a phytochemical study on Calligonum azel and Calligonum comosum which concerned mainly the biologically active compounds then we tested the antioxidant, antibacterial, antifungal and anti-inflammatory properties of all the organic extracts and some isolated compounds. Secondly; we studied the chemical composition and biological activities of Thymus ciliatus essential oil. The study of aerial parts of C. azel and C. comosum, in order to know the active compounds responsible for their biological activity, leading to purification, chromatographic identification (CC, TLC and HPLC), and characterization using the different spectroscopic methods (1D and 2D NMR, MS and UV) of twenty one pure compounds (21) in which eighteen (18) compounds were identified. The investigation of the biological activities of the extracts of Calligonum azel and Calligonum comosum showed that the best antioxidant activity was exhibited by the Chloroform and ethyl acetate extracts of C. azel and the Chloroform extract of C. comosum. The chloroform extracts of both species showed the best antibacterial activity. Chloroform extract from C. comosum has better antifungal activity against Candida albicans ATCC 2019 and Candida parapsilosis ATCC 22019. From the isolated compounds, three have an antioxidant and anti-inflammatory activity. These compounds were: protocatachuic ethyl ester acid, -diamino (4-hydroxy benzene) butanoic acid and tamgernanetin. The qualitative and quantitative analysis of essential oil of T. ciliatus allows the identification of 25 compounds representing 87.18 % of the total essential oil mainly. The GC and GC-MS analysis showed that this essential oils was mainly rich of thymol (54.26%), carvacrol methyl ether (1,373 %), o-cymene (5.72 %) carvacrol (1.373%)-cymen-7-ol (17.58 %), however it was the first time that -cymen-7-ol (17.58 %) was found as main component in the essential oils of T. ciliatus. The evaluation of the antibacterial activity was assessed by disc diffusion method against bacterial strains. The essential oil exhibited a good antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 43300 and Staphylocossus aureus. All of our works confirms the medicinal properties of the Saharian species, and contributes to their scientific descriptions. Diplôme : Doctorat En ligne : ../theses/chimie/BEL7311.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10926 Etude chimique et biologique des espèces Calligonum azel et Calligonum comosum (Polygonaceae) et une espèce du genre Thymus (Lamiaceae). [texte imprimé] / Soumia Belaabed Khalfaoui, Auteur ; Nour-eddine Beghidja, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2018 . - 250 f. ; 30 cm. 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais Chimie Tags : Calligonum azel  Calligonum comosum  Thymus ciliatus  Polygonaceae  Lamiaceae  RMN 1D et 2D  CLHP  HRESIMS  activités biologiques  huile essentielle  NMR 1D and 2D  HPLC  Biological activities  essential oil  ??????? ?????? ??????????1D ?2D  ????????????? HPLC  ??????? ??????  ???????? ??????????  ?????? ???????? Index. décimale : 540 Chimie et sciences connexes Résumé : The phytochemical and biological study concerns the aerial parts of two endemic Saharian species belonging to the polygonaceae family: Calligonum azel and Calligonum comosum and a third one belonging to the Lamiaceae family. The object of our work focused on the valorization of the Algerian flora. For this, firstly; we carried out a phytochemical study on Calligonum azel and Calligonum comosum which concerned mainly the biologically active compounds then we tested the antioxidant, antibacterial, antifungal and anti-inflammatory properties of all the organic extracts and some isolated compounds. Secondly; we studied the chemical composition and biological activities of Thymus ciliatus essential oil. The study of aerial parts of C. azel and C. comosum, in order to know the active compounds responsible for their biological activity, leading to purification, chromatographic identification (CC, TLC and HPLC), and characterization using the different spectroscopic methods (1D and 2D NMR, MS and UV) of twenty one pure compounds (21) in which eighteen (18) compounds were identified. The investigation of the biological activities of the extracts of Calligonum azel and Calligonum comosum showed that the best antioxidant activity was exhibited by the Chloroform and ethyl acetate extracts of C. azel and the Chloroform extract of C. comosum. The chloroform extracts of both species showed the best antibacterial activity. Chloroform extract from C. comosum has better antifungal activity against Candida albicans ATCC 2019 and Candida parapsilosis ATCC 22019. From the isolated compounds, three have an antioxidant and anti-inflammatory activity. These compounds were: protocatachuic ethyl ester acid, -diamino (4-hydroxy benzene) butanoic acid and tamgernanetin. The qualitative and quantitative analysis of essential oil of T. ciliatus allows the identification of 25 compounds representing 87.18 % of the total essential oil mainly. The GC and GC-MS analysis showed that this essential oils was mainly rich of thymol (54.26%), carvacrol methyl ether (1,373 %), o-cymene (5.72 %) carvacrol (1.373%)-cymen-7-ol (17.58 %), however it was the first time that -cymen-7-ol (17.58 %) was found as main component in the essential oils of T. ciliatus. The evaluation of the antibacterial activity was assessed by disc diffusion method against bacterial strains. The essential oil exhibited a good antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 43300 and Staphylocossus aureus. All of our works confirms the medicinal properties of the Saharian species, and contributes to their scientific descriptions. Diplôme : Doctorat En ligne : ../theses/chimie/BEL7311.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10926

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
BEL/7311	BEL/7311	Thèse	Bibliothèque principale	Thèses	Disponible

Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien / Samira Malki  

Public ISBD Titre : Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien Type de document : texte imprimé Auteurs : Samira Malki, Auteur ; A Yahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 186 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais Biologie Tags : Teucrium polium L. Capitatum  Lamiaceae  trichome  électrophorèse  CLHP  polyphenols  activité antioxydante  activité antibactérienne  effet diurétique  trichomes  electrophoresis  HPLC  antioxidant activity  antibacterial activity  diuretic effect  ??????? ???????  ???????  ????? ?????????  ??????? ??????? ???????  ??????? ??????? ?????????  ??? ????? ????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of our work has focused on the study of Teucrium polium L. Capitatum, which is a widespread medicinal plant in algerian steppes. Several study topics were investigated such as the morphology, physio-biochemical constituents and biological effects. The morphological analysis consisted of ten populations sampled at different habitats in eastern Algeria. This study revealed that the leaves have the lowest number (five out of ten) of types of trichomes, followed by the calyx and corolla with eight types for each. This confirms the absence of intraspecific variability within the study populations. Furthermore, the analysis of relationships between morphological characteristics and environmental factors of their habitat showed that the latter influences the length of trichomes. The use of one-dimensional electrophoresis technique on polyacrylamide gel in the presence of detergent (SDS-PAGE) allowed to determine the total protein profiles of the ten study populations. The similarity coefficients of polypeptide bands indicated high similarity between populations. Biochemical analyzes of the three phenotypes of T. polium L. Capitatum showed that extracts of purple phenotypes were richest in anthocyanins, soluble sugars and carotenoids compared with pink and white phenotypes enlightening there by the importance of flower color. The combination of certain chemical analysis methods, spectrophotometry (UV-visible) and chromatographic (HPLC) have identified three flavonoids including mostly the cyanidin and two other flavonoids, quercetin and caffeic acid. The evaluation of the antioxidant potency, that was conducted using the method of trapping the free radical DPPH and that of iron reduction FRAP, revealed that the extract of Biskra population showed higher reducing capacity compared to that of Sétif, with a strong trapping of DPPH● radicals. As regards to the antibacterial activity, it appears that extracts from two populations have acceptable antibacterial properties. The pharmacological effect of T. polium L. Capitatum showed an interesting diuretic property. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAL6998.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10396 Etude morphologique, biochimique, physiologique et biologique de quelques populations de Teucrium polium L. Capitatum dans l’Est algérien [texte imprimé] / Samira Malki, Auteur ; A Yahia, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 186 f. ; 30 cm. 2 copies imprimées disponibles Langues : Français (fre) Catégories : Français - Anglais Biologie Tags : Teucrium polium L. Capitatum  Lamiaceae  trichome  électrophorèse  CLHP  polyphenols  activité antioxydante  activité antibactérienne  effet diurétique  trichomes  electrophoresis  HPLC  antioxidant activity  antibacterial activity  diuretic effect  ??????? ???????  ???????  ????? ?????????  ??????? ??????? ???????  ??????? ??????? ?????????  ??? ????? ????? Index. décimale : 570 Sciences de la vie. Biologie Résumé : The purpose of our work has focused on the study of Teucrium polium L. Capitatum, which is a widespread medicinal plant in algerian steppes. Several study topics were investigated such as the morphology, physio-biochemical constituents and biological effects. The morphological analysis consisted of ten populations sampled at different habitats in eastern Algeria. This study revealed that the leaves have the lowest number (five out of ten) of types of trichomes, followed by the calyx and corolla with eight types for each. This confirms the absence of intraspecific variability within the study populations. Furthermore, the analysis of relationships between morphological characteristics and environmental factors of their habitat showed that the latter influences the length of trichomes. The use of one-dimensional electrophoresis technique on polyacrylamide gel in the presence of detergent (SDS-PAGE) allowed to determine the total protein profiles of the ten study populations. The similarity coefficients of polypeptide bands indicated high similarity between populations. Biochemical analyzes of the three phenotypes of T. polium L. Capitatum showed that extracts of purple phenotypes were richest in anthocyanins, soluble sugars and carotenoids compared with pink and white phenotypes enlightening there by the importance of flower color. The combination of certain chemical analysis methods, spectrophotometry (UV-visible) and chromatographic (HPLC) have identified three flavonoids including mostly the cyanidin and two other flavonoids, quercetin and caffeic acid. The evaluation of the antioxidant potency, that was conducted using the method of trapping the free radical DPPH and that of iron reduction FRAP, revealed that the extract of Biskra population showed higher reducing capacity compared to that of Sétif, with a strong trapping of DPPH● radicals. As regards to the antibacterial activity, it appears that extracts from two populations have acceptable antibacterial properties. The pharmacological effect of T. polium L. Capitatum showed an interesting diuretic property. Diplôme : Doctorat en sciences En ligne : ../theses/biologie/MAL6998.pdf Format de la ressource électronique : pdf Permalink : https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10396

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
MAL/6998	MAL/6998	Thèse	Bibliothèque principale	Thèses	Disponible