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Partager le résultat de cette recherche Interroger des sources externesEffect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process / Assia Benmebarek
Titre : Effect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process Type de document : texte imprimé Auteurs : Assia Benmebarek, Auteur ; S Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2014 Importance : 231 f. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Hyperhomocysteinemia atherogenesis inflammation carcinogenesis Stachys mialhesi Stachys ocymastrum immunomodulatory activity Hyperhomocysteinémie athérogénèse carcinogénèse activité immunomodulatrice زیادة الھوموسستیین البلازمي تصلب الشرایین الالتھاب السرطان التنبیھ المناعي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia is an important risk factor in cardiovascular diseases and may also be implicated in cancer biology. Elevated plasma homocysteine (Hcy) levels may mediate long-term oxidative damage at the vascular interface and there appear to be a mechanism by which they may also be carcinogenic. In addition, immunostimulatory therapy is one solution to enhance the immunity, however, the immunopharmacologic activities of herbal extracts may be antagonistic, they can be immunosupressive or immunostimulating. In the present study, we evaluated the in vivo effect of S. mialhesi extract on the inflammation induced by hyperhomocysteinemia and damages induced to the aorta, heart, liver, thymus and spleen. Another study was carried on the immunomodulatory potential of S. mialhesi
and S. ocymastrum on the phagocytic activity which was evaluated using the Carbon Clearance Assay. The results obtained showed that plasma hs-CRP concentrations were elevated significantly after the administration of L-methionine in high doses to mice (200mg/kg). Also, the loss and degeneration of endothelium, fenestration, foam cells formation, and a change in the appearance of smooth muscle cells nuclei from a fusiform nuclei aspect to a rounded one, were observed in the media of the aorta as well as the alteration of the cardiac muscle, liver necrosis, lymphocytes membrane destruction in the thymus, and a hypertrophy of the spleen white pulp. Our research also revealed that at different doses, S. mialhesi extract increased the reticuloendothelial system (RES)
phagocytic activity in a dose dependant manner, in contrast to S. ocymastrum who exhibited a biphasic dose response effect. Hcy initiated inflammation and mediated early atherogenesis lesions through increased oxidant stress, and the treatment with S. mialhesi extract at a dose of (50mg/kg) prevented the alterations. Also, S. mialhesi extract exhibited a dose-dependent immunostimulatory effect on the RES and S. ocymastrum extract appeared immunostimulatory at low concentrations and immunosuppressive at high concentrations. Moreover, in the present thesis, we performed a review study which demonstrated that Hcy may mediate carcinogenesis namely through folate deficiency, oxidative stress, aberrant DNA methylation and the production of homocysteine thiolactone.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6973.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10371 Effect of bioactive molecules extracted from medicinal plants on inflammation induced by hyperhomocysteinemia and tumoral process [texte imprimé] / Assia Benmebarek, Auteur ; S Zerizer, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2014 . - 231 f.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Hyperhomocysteinemia atherogenesis inflammation carcinogenesis Stachys mialhesi Stachys ocymastrum immunomodulatory activity Hyperhomocysteinémie athérogénèse carcinogénèse activité immunomodulatrice زیادة الھوموسستیین البلازمي تصلب الشرایین الالتھاب السرطان التنبیھ المناعي Index. décimale : 570 Sciences de la vie. Biologie Résumé : Hyperhomocysteinemia is an important risk factor in cardiovascular diseases and may also be implicated in cancer biology. Elevated plasma homocysteine (Hcy) levels may mediate long-term oxidative damage at the vascular interface and there appear to be a mechanism by which they may also be carcinogenic. In addition, immunostimulatory therapy is one solution to enhance the immunity, however, the immunopharmacologic activities of herbal extracts may be antagonistic, they can be immunosupressive or immunostimulating. In the present study, we evaluated the in vivo effect of S. mialhesi extract on the inflammation induced by hyperhomocysteinemia and damages induced to the aorta, heart, liver, thymus and spleen. Another study was carried on the immunomodulatory potential of S. mialhesi
and S. ocymastrum on the phagocytic activity which was evaluated using the Carbon Clearance Assay. The results obtained showed that plasma hs-CRP concentrations were elevated significantly after the administration of L-methionine in high doses to mice (200mg/kg). Also, the loss and degeneration of endothelium, fenestration, foam cells formation, and a change in the appearance of smooth muscle cells nuclei from a fusiform nuclei aspect to a rounded one, were observed in the media of the aorta as well as the alteration of the cardiac muscle, liver necrosis, lymphocytes membrane destruction in the thymus, and a hypertrophy of the spleen white pulp. Our research also revealed that at different doses, S. mialhesi extract increased the reticuloendothelial system (RES)
phagocytic activity in a dose dependant manner, in contrast to S. ocymastrum who exhibited a biphasic dose response effect. Hcy initiated inflammation and mediated early atherogenesis lesions through increased oxidant stress, and the treatment with S. mialhesi extract at a dose of (50mg/kg) prevented the alterations. Also, S. mialhesi extract exhibited a dose-dependent immunostimulatory effect on the RES and S. ocymastrum extract appeared immunostimulatory at low concentrations and immunosuppressive at high concentrations. Moreover, in the present thesis, we performed a review study which demonstrated that Hcy may mediate carcinogenesis namely through folate deficiency, oxidative stress, aberrant DNA methylation and the production of homocysteine thiolactone.
Diplôme : Doctorat En ligne : ../theses/biologie/BEN6973.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10371 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BEN/6973 BEN/6973 Thèse Bibliothèque principale Thèses Disponible
Titre : Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process Type de document : texte imprimé Auteurs : Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 173 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE.
2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
BiologieTags : Animal Biology:Immuno-Oncology Phoenix dactylifera activité immunomodulatrice glutathion réduit inflammation hyperhomocystéinémie activité anti-arthrite cancer immunomodulatory activity GSH glutathione hyperhomocysteinemia anti-arthritis activity التمر dactylifera Phoenix النشاط المناعي الجلوثيونGSH الإلتهاب فرط الهوموسستئين مضاد التهاب المفاصل السرطان Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine.
In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix
dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg.
The results showed that the extracts of Phoenix dactylifera have no toxic effect at a
dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values.
The results indicate that treatment with six different concentrations (2 mg/100mL, 4
mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the
growth of tumor cells but had no toxicity to healthy cells.
Note de contenu : Article 1:IMMUNOSTIMULATORY ACTIVITY OF PHOENIX DACTYLIFERA KEHILI HOUSSEM EDDINE¹, SAKINA ZERIZER*¹, ZAHIA KABOUCHE² .
Article 2:Anti-inflammatory effect of Algerian date fruit(Phoenix dactylifera)Houssem Eddine Kehili, Sakina Zerizer, Khadidja Aya Beladjila & ZahiaKabouche.
ملخص بالعربية للمطول للرسالة
Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10409 Biological activities of Phoenix dactylifera and Treg in Rheumatoid arthritis induced by hyperhomocysteinemia and formalin and on tumoral process [texte imprimé] / Houssem Eddine Kehili, Auteur ; sakina Zerizer, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 173 f. ; 30 cm.
Doctorat 3éme CYCLE.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
BiologieTags : Animal Biology:Immuno-Oncology Phoenix dactylifera activité immunomodulatrice glutathion réduit inflammation hyperhomocystéinémie activité anti-arthrite cancer immunomodulatory activity GSH glutathione hyperhomocysteinemia anti-arthritis activity التمر dactylifera Phoenix النشاط المناعي الجلوثيونGSH الإلتهاب فرط الهوموسستئين مضاد التهاب المفاصل السرطان Index. décimale : 570 Sciences de la vie. Biologie Résumé : Inflammation plays an important role in various diseases, such as rheumatoid arthritis, atherosclerosis and asthma, which all show a high prevalence globally and the absence of the immune tolerance which is insured by the lymphocyte Treg. This tolerance protects the selfantigens from the immune system’s reaction. In addition, some chronic inflammatory diseases are recently found to be associated with an increased levels of hs-CRP and plasma homocysteine.
In the present study, we evaluated the in vivo, the effect of two varieties of Phoenix
dactylifera (Azarza variety grown in Ghardaia and variety Homayra grown in Adrar) on the toxicity using the up and down test, the immunomodulatory activity of the extracts using the carbon clearance from the blood, antioxidant by the measurement of the GSH from liver’s homogenate, anti-inflammatory activity and anti-arthritis by the formalin and L-methionine test in Albino mice. Also we have carried a study in vitro to establish the anti-proliferative effect of the methanolic and acetone extracts dates on liver cancer cell line (HepG2), breast cancer cell line (MCF7) and healthy cells, endothelial cells (HUVEC) and hepatocytes (hNHEPS). In addition to this work, we tested the effect of Phoenix dactylifera extracts on apoptotic genes (Bcl2 and BAX) and on the differentiation of human naïve lymphocyte T CD4+ into regulatory lymphocytes Treg.
The results showed that the extracts of Phoenix dactylifera have no toxic effect at a
dose of 2000mg/kg, also we found that the extracts increased significantly the phagocytic activity of the reticuloendothelial system, and release of glutathione reduced (GSH) from the liver. Furthermore, the extracts of Phoenix dactylifera studied reveal correction on the inflammation associated with hyperhomocysteinemia presented by a significant decrease in the size of the edema induced by formalin injection and a significant decrease in the hs- CRPvalues and homocysteine Hcy (p ≤ 0.05) in mice treated compared to controls, where it was observed that the administration of L-methionine 400 mg/kg caused a worsening of inflammation presented by a significant increase in protein C-reactive (CRP) p≤0,05 and a significant increase in homocysteine (Hcy) p≤0,05. Our results demonstrate a significant inhibition of edema of mice paws treated with our extracts with a decrease in the Anti-CCP values.
The results indicate that treatment with six different concentrations (2 mg/100mL, 4
mg/100mL, 7.5 mg/100mL, 8 mg/100mL, 16 mg/1 00mL and 20 mg/100mL) inhibited the
growth of tumor cells but had no toxicity to healthy cells.
Note de contenu : Article 1:IMMUNOSTIMULATORY ACTIVITY OF PHOENIX DACTYLIFERA KEHILI HOUSSEM EDDINE¹, SAKINA ZERIZER*¹, ZAHIA KABOUCHE² .
Article 2:Anti-inflammatory effect of Algerian date fruit(Phoenix dactylifera)Houssem Eddine Kehili, Sakina Zerizer, Khadidja Aya Beladjila & ZahiaKabouche.
ملخص بالعربية للمطول للرسالة
Diplôme : Doctorat En ligne : ../theses/biologie/KEH6985.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10409 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KEH/6985 KEH/6985 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur de la plante Linaria tingitana (Scrophulariaceae) sur le dysfonctionnement mitochondrial et lysosomal hépatocytaire induit par l’acide valproïque chez le rat. Type de document : texte imprimé Auteurs : Mourad Hanfer, Auteur ; Souad Ameddah, Directeur de thèse ; Didier Morin, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 202 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Valproïc acid Linaria tingitana LC-MS. Mitochondrial dysfunction Lysosomal dysfunction Rats HepG2 Inflammation Oxidative stress Acide valproïque LC-MS. Dysfonctionnement mitochondrial Dysfonctionnement lysosomal Stress oxydant Index. décimale : 570 Sciences de la vie. Biologie Résumé : This investigation is interested to study the modulator effect of Linaria tingitana in rats
treated by the valproic acid (VPA). The in vitro studies (DPPH◦, FRAP, β-carotene, Chelation, NO◦) showed that Linaria tingitana is endowed with an antioxidant property. The antiinflammatory studies (albumin denaturation, HRBC, NO scavenger, oedema and peritonitis led
by the carrageenan, the rise of MPO and the infiltration of the PN) confirmed that Linaria
tingitana has a beneficial effect in this sense. The in vivo studies made to rats handled by the
VPA revealed that the n-BuOH extract acts by a multitude of mechanisms: conservation of the
hepatic function, the modulation of the steatoses, the neutralization of the oxidative stress and
the reduction of the inflammation hepatocyte elucidated by the decrease of the activity MPO.
Antirrhides molecules isolated of this plant were ineffective at the level of the cell line HepG2
treated with the VPA. The mitochondrial dysfunction led by the VPA is expressed by the decrease of the RCR accompanied by a decrease of the activity of the complex I. The n-BuOH
extract as well as the isolated molecules managed to reduce the mitochondrial H2O2 and to modulate the antioxidant mitochondrial and lysosomal systems. The conservation of the integrity
lysosomal membrane was more pronounced by the n-BuOH extract compared to the vitamin
E. The various modulator ways of L. tingitana delayed the appearance of the complications
linked to the mitochondrial dysfunction, lysosomal, such necrosis. Probably this is due to the
diversified wealth of this plant in term of polyphenols and irridoids revealed by phytochemical
screening and the analysis of the LC-MS and in the synergetic effect of the molecules that it
contains.Diplôme : Doctorat En ligne : ../theses/biologie/HAN7131.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10471 L’effet protecteur de la plante Linaria tingitana (Scrophulariaceae) sur le dysfonctionnement mitochondrial et lysosomal hépatocytaire induit par l’acide valproïque chez le rat. [texte imprimé] / Mourad Hanfer, Auteur ; Souad Ameddah, Directeur de thèse ; Didier Morin, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 202 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Valproïc acid Linaria tingitana LC-MS. Mitochondrial dysfunction Lysosomal dysfunction Rats HepG2 Inflammation Oxidative stress Acide valproïque LC-MS. Dysfonctionnement mitochondrial Dysfonctionnement lysosomal Stress oxydant Index. décimale : 570 Sciences de la vie. Biologie Résumé : This investigation is interested to study the modulator effect of Linaria tingitana in rats
treated by the valproic acid (VPA). The in vitro studies (DPPH◦, FRAP, β-carotene, Chelation, NO◦) showed that Linaria tingitana is endowed with an antioxidant property. The antiinflammatory studies (albumin denaturation, HRBC, NO scavenger, oedema and peritonitis led
by the carrageenan, the rise of MPO and the infiltration of the PN) confirmed that Linaria
tingitana has a beneficial effect in this sense. The in vivo studies made to rats handled by the
VPA revealed that the n-BuOH extract acts by a multitude of mechanisms: conservation of the
hepatic function, the modulation of the steatoses, the neutralization of the oxidative stress and
the reduction of the inflammation hepatocyte elucidated by the decrease of the activity MPO.
Antirrhides molecules isolated of this plant were ineffective at the level of the cell line HepG2
treated with the VPA. The mitochondrial dysfunction led by the VPA is expressed by the decrease of the RCR accompanied by a decrease of the activity of the complex I. The n-BuOH
extract as well as the isolated molecules managed to reduce the mitochondrial H2O2 and to modulate the antioxidant mitochondrial and lysosomal systems. The conservation of the integrity
lysosomal membrane was more pronounced by the n-BuOH extract compared to the vitamin
E. The various modulator ways of L. tingitana delayed the appearance of the complications
linked to the mitochondrial dysfunction, lysosomal, such necrosis. Probably this is due to the
diversified wealth of this plant in term of polyphenols and irridoids revealed by phytochemical
screening and the analysis of the LC-MS and in the synergetic effect of the molecules that it
contains.Diplôme : Doctorat En ligne : ../theses/biologie/HAN7131.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10471 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAN/7131 HAN/7131 Thèse Bibliothèque principale Thèses Disponible
Titre : Etude du rôle de Monoxyde d’azote (NO) dans la progression du myélome Multiple (MM) et son implication dans le développement des maladies osseuses associées au MM. Type de document : texte imprimé Auteurs : Khawla Otmani, Auteur ; Dalila Naimi, Directeur de thèse ; Nadia Boudersa, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2019 Importance : 164 f. Format : 30 cm. Note générale : Doctorat 3éme CYCLE LMD.
2 copies imprimées disponiblesLangues : Français (fre) Catégories : Français - Anglais
BiologieTags : Biologie Animale: Immuno-Oncologie inflammation monoxyde d'azote (NO) myélome multiple (MM) le microenvironnement de MM myeloma plasma cells (MPC) Les maladies osseuses associées au MM (MBD) Marqueur pronostique de MM marqueur de remodelage osseux ostéoblaste nitric oxide (NO) multiple myeloma (MM) myeloma microenvironment (MPC)
myeloma plasma cells meyeloma bone deseases MBD Myeloma prognostic marker bone remodling marker INOS inhibitor osteoblast الالتهاب اكسيد النيتريك (NO) الورم النخاعي المتعدد (MM) الخلايا البلازمية لورم النخاعي المتعدد (MPC) امراض عظام الورم النخاعي المتعدد (MBD) مؤشرات التطور مؤشرات إعادة تشكيل العظم الاستوبلاستات ( osteoblastesIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Nitric oxide (NO) is sensitive marker of inflammation and it is widely associated with cancer development. Multiple myeloma (MM) is a hematological cancer of fully differentiated plasma cells in the bone marrow which is a favorable microenvironment for the promotion of communication between malignant plasma cells MPC (Myeloma plasma cells) and other cell types using pro-inflammatory mediators. Myeloma bone diseases (MBD) and monoclonal mmunoglobulin secretion are the most visible aspects in MM.The present work aims to study the role of (NO) as an inflammatory mediator in the progression of MM and its effect on the development of MBD. We evaluated in vivo, the NO concentration in the plasma of MM patients in the Algerian East, and its possible correlation with some prognostic marker evolution, the statue of the immune
system, and some bone remodeling markers in these patients. We have also carried out a study in vitro to evaluate NO production by myeloma microenvironment using MPC (RPMI 8226 cell lines) co-culture withe osteoblast ( MG63 cell line) and to investigate anti-proliferative and cytotoxic effect of iNOS inhibitor on the two cell lines co-culture . The results showed a significant increase of NO concentration in the plasma of MM patients comparing with the plasma of positive and negative controls (p> 0.01). Hence, the profile of NO concentration allowed the revealing of a slight relation with some prognostic and bone remolding markers. Furthermore we found a significant relationship between NO concentration and monocytes (p> 0.01) as well as granulocytes rate (p> 0.01) in the MM patients. In addition, the studies realized in vitro confirmed an important production of NO by myeloma cell line (RPMI 8226), which was also noticed in the case of the co-culture of myeloma cell line (RPMI 8226 ) with the osteoblast (MG63). This work has elucidated the role of NO in MM cancer and its effect on some manifestations of the disease, including MBD.Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/OTM7501.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11333 Etude du rôle de Monoxyde d’azote (NO) dans la progression du myélome Multiple (MM) et son implication dans le développement des maladies osseuses associées au MM. [texte imprimé] / Khawla Otmani, Auteur ; Dalila Naimi, Directeur de thèse ; Nadia Boudersa, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2019 . - 164 f. ; 30 cm.
Doctorat 3éme CYCLE LMD.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Biologie Animale: Immuno-Oncologie inflammation monoxyde d'azote (NO) myélome multiple (MM) le microenvironnement de MM myeloma plasma cells (MPC) Les maladies osseuses associées au MM (MBD) Marqueur pronostique de MM marqueur de remodelage osseux ostéoblaste nitric oxide (NO) multiple myeloma (MM) myeloma microenvironment (MPC)
myeloma plasma cells meyeloma bone deseases MBD Myeloma prognostic marker bone remodling marker INOS inhibitor osteoblast الالتهاب اكسيد النيتريك (NO) الورم النخاعي المتعدد (MM) الخلايا البلازمية لورم النخاعي المتعدد (MPC) امراض عظام الورم النخاعي المتعدد (MBD) مؤشرات التطور مؤشرات إعادة تشكيل العظم الاستوبلاستات ( osteoblastesIndex. décimale : 570 Sciences de la vie. Biologie Résumé : Nitric oxide (NO) is sensitive marker of inflammation and it is widely associated with cancer development. Multiple myeloma (MM) is a hematological cancer of fully differentiated plasma cells in the bone marrow which is a favorable microenvironment for the promotion of communication between malignant plasma cells MPC (Myeloma plasma cells) and other cell types using pro-inflammatory mediators. Myeloma bone diseases (MBD) and monoclonal mmunoglobulin secretion are the most visible aspects in MM.The present work aims to study the role of (NO) as an inflammatory mediator in the progression of MM and its effect on the development of MBD. We evaluated in vivo, the NO concentration in the plasma of MM patients in the Algerian East, and its possible correlation with some prognostic marker evolution, the statue of the immune
system, and some bone remodeling markers in these patients. We have also carried out a study in vitro to evaluate NO production by myeloma microenvironment using MPC (RPMI 8226 cell lines) co-culture withe osteoblast ( MG63 cell line) and to investigate anti-proliferative and cytotoxic effect of iNOS inhibitor on the two cell lines co-culture . The results showed a significant increase of NO concentration in the plasma of MM patients comparing with the plasma of positive and negative controls (p> 0.01). Hence, the profile of NO concentration allowed the revealing of a slight relation with some prognostic and bone remolding markers. Furthermore we found a significant relationship between NO concentration and monocytes (p> 0.01) as well as granulocytes rate (p> 0.01) in the MM patients. In addition, the studies realized in vitro confirmed an important production of NO by myeloma cell line (RPMI 8226), which was also noticed in the case of the co-culture of myeloma cell line (RPMI 8226 ) with the osteoblast (MG63). This work has elucidated the role of NO in MM cancer and its effect on some manifestations of the disease, including MBD.Note de contenu : Annexes. Diplôme : Doctorat En ligne : ../theses/biologie/OTM7501.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11333 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité OTM/7501 OTM/7501 Thèse Bibliothèque principale Thèses Disponible
Titre : Caractérisation et étude des propriétés immunomodulatrices, anti-inflammatoires et anti-tumorales des molécules bioactives extraites d’Helix aspersa : application dans la prévention du cancer colorectal expérimental chimio induit. Type de document : texte imprimé Auteurs : Meriem Kouachi, Auteur ; Dalila Naimi, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 125 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles.
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Helix aspersa bave homogénat extrait aqueux immunostimulation inflammation prolifération tumorale slime homogenate aqueous extract tumor proliferation الحلزون هيليكس اسبيرس لعاب، جناسة مستخلص المائي تحفيزمناعي التهاب ورم Index. décimale : 570 Sciences de la vie. Biologie Résumé : A competent immune system is capable of detecting and eliminating not only viruses and bacteria, but also cancer cells. However, these cells can develop an escape strategy allowing the formation of malignant tumor following immunosuppression. It is therefore necessary to look for new anti-tumor drugs with an immunostimulatory potential. Research in this field focuses on the extraction and purification of these molecules from medicinal plants. However, the aim of our work is to seek and demonstrate the immunomodulatory effect of animal extracts: the Helix aspersa snail, a species widely used and widely consumed in the Mediterranean countries. Our work begins with a characterization of the extracts (slime, homogenate and aqueous extract) and an evaluation of the toxic dose in order to choose the appropriate doses to use during our experiments. The extracts showed no toxicity to experimental animals; Therefore, the doses of 20 ml / Kg of slime, 5 mg / Kg of homogenate and 300 μl / mouse of aqueous extract were chosen. The immunomodulatory effect was shown to be immunostimulatory when performing a carbon clearance test, which led us to investigate other therapeutic effects. Our extracts also exert an anti-inflammatory effect against chemo-induced experimental colitis. Untreated chronic colitis is likely to develop as a colorectal tumor. The anti-inflammatory effect of the extracts prompted us to check the antiproliferative effect. The investigation of the antitumor effect was carried out in vitro, on a culture of MCF7 mammary tumor cells and in vivo, on an experimental chemo-induced colorectal cancer. In vitro experiments showed an inhibition of tumor cell proliferation at 56.39%. In vivo, observation of the histological sections of the colonists confirmed that the extracts repaired the architecture of the colonic mucosa after the installation of the chemoinduced tumor. The Helix aspersa snail has several therapeutic effects. It would therefore be interesting to introduce it into the daily diet as a preventive or supplementary food for a curative purpose.
Diplôme : Doctorat En ligne : ../theses/biologie/KOU7168.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10589 Caractérisation et étude des propriétés immunomodulatrices, anti-inflammatoires et anti-tumorales des molécules bioactives extraites d’Helix aspersa : application dans la prévention du cancer colorectal expérimental chimio induit. [texte imprimé] / Meriem Kouachi, Auteur ; Dalila Naimi, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 125 f. ; 30 cm.
2 copies imprimées disponibles.
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Helix aspersa bave homogénat extrait aqueux immunostimulation inflammation prolifération tumorale slime homogenate aqueous extract tumor proliferation الحلزون هيليكس اسبيرس لعاب، جناسة مستخلص المائي تحفيزمناعي التهاب ورم Index. décimale : 570 Sciences de la vie. Biologie Résumé : A competent immune system is capable of detecting and eliminating not only viruses and bacteria, but also cancer cells. However, these cells can develop an escape strategy allowing the formation of malignant tumor following immunosuppression. It is therefore necessary to look for new anti-tumor drugs with an immunostimulatory potential. Research in this field focuses on the extraction and purification of these molecules from medicinal plants. However, the aim of our work is to seek and demonstrate the immunomodulatory effect of animal extracts: the Helix aspersa snail, a species widely used and widely consumed in the Mediterranean countries. Our work begins with a characterization of the extracts (slime, homogenate and aqueous extract) and an evaluation of the toxic dose in order to choose the appropriate doses to use during our experiments. The extracts showed no toxicity to experimental animals; Therefore, the doses of 20 ml / Kg of slime, 5 mg / Kg of homogenate and 300 μl / mouse of aqueous extract were chosen. The immunomodulatory effect was shown to be immunostimulatory when performing a carbon clearance test, which led us to investigate other therapeutic effects. Our extracts also exert an anti-inflammatory effect against chemo-induced experimental colitis. Untreated chronic colitis is likely to develop as a colorectal tumor. The anti-inflammatory effect of the extracts prompted us to check the antiproliferative effect. The investigation of the antitumor effect was carried out in vitro, on a culture of MCF7 mammary tumor cells and in vivo, on an experimental chemo-induced colorectal cancer. In vitro experiments showed an inhibition of tumor cell proliferation at 56.39%. In vivo, observation of the histological sections of the colonists confirmed that the extracts repaired the architecture of the colonic mucosa after the installation of the chemoinduced tumor. The Helix aspersa snail has several therapeutic effects. It would therefore be interesting to introduce it into the daily diet as a preventive or supplementary food for a curative purpose.
Diplôme : Doctorat En ligne : ../theses/biologie/KOU7168.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10589 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KOU/7168 KOU/7168 Thèse Bibliothèque principale Thèses Disponible
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