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Partager le résultat de cette recherche Interroger des sources externesL’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque / Amina Maya Lahneche
Titre : L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo Type de document : texte imprimé Auteurs : Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 140 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds التوتر التأكسدي الفعل المضاد للأكسدة المستخلص النباتي المركبات الفينولية الأكسدة الفوقية للدهونIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10833 L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque : Etude in vitro et in vivo [texte imprimé] / Amina Maya Lahneche, Auteur ; Djamila Zama, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 140 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Centaurea sphaerocephala l’acide valproïque stress oxydative activités antioxydantes composés phénoliques oxidative stress valproic acid antioxidant activities lipid
peroxidation plant extract phenolic compounds التوتر التأكسدي الفعل المضاد للأكسدة المستخلص النباتي المركبات الفينولية الأكسدة الفوقية للدهونIndex. décimale : 570 Sciences de la vie. Biologie Résumé : This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination.
The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated.
As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular.
Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied.
n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect.
The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high.
The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.
Diplôme : Doctorat En ligne : ../theses/biologie/LAH7238.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10833 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAH/7238 LAH/7238 Thèse Bibliothèque principale Thèses Disponible
Titre : L’effet protecteur de la plante Linaria tingitana (Scrophulariaceae) sur le dysfonctionnement mitochondrial et lysosomal hépatocytaire induit par l’acide valproïque chez le rat. Type de document : texte imprimé Auteurs : Mourad Hanfer, Auteur ; Souad Ameddah, Directeur de thèse ; Didier Morin, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 202 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
BiologieTags : Valproïc acid Linaria tingitana LC-MS. Mitochondrial dysfunction Lysosomal dysfunction Rats HepG2 Inflammation Oxidative stress Acide valproïque LC-MS. Dysfonctionnement mitochondrial Dysfonctionnement lysosomal Stress oxydant Index. décimale : 570 Sciences de la vie. Biologie Résumé : This investigation is interested to study the modulator effect of Linaria tingitana in rats
treated by the valproic acid (VPA). The in vitro studies (DPPH◦, FRAP, β-carotene, Chelation, NO◦) showed that Linaria tingitana is endowed with an antioxidant property. The antiinflammatory studies (albumin denaturation, HRBC, NO scavenger, oedema and peritonitis led
by the carrageenan, the rise of MPO and the infiltration of the PN) confirmed that Linaria
tingitana has a beneficial effect in this sense. The in vivo studies made to rats handled by the
VPA revealed that the n-BuOH extract acts by a multitude of mechanisms: conservation of the
hepatic function, the modulation of the steatoses, the neutralization of the oxidative stress and
the reduction of the inflammation hepatocyte elucidated by the decrease of the activity MPO.
Antirrhides molecules isolated of this plant were ineffective at the level of the cell line HepG2
treated with the VPA. The mitochondrial dysfunction led by the VPA is expressed by the decrease of the RCR accompanied by a decrease of the activity of the complex I. The n-BuOH
extract as well as the isolated molecules managed to reduce the mitochondrial H2O2 and to modulate the antioxidant mitochondrial and lysosomal systems. The conservation of the integrity
lysosomal membrane was more pronounced by the n-BuOH extract compared to the vitamin
E. The various modulator ways of L. tingitana delayed the appearance of the complications
linked to the mitochondrial dysfunction, lysosomal, such necrosis. Probably this is due to the
diversified wealth of this plant in term of polyphenols and irridoids revealed by phytochemical
screening and the analysis of the LC-MS and in the synergetic effect of the molecules that it
contains.Diplôme : Doctorat En ligne : ../theses/biologie/HAN7131.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10471 L’effet protecteur de la plante Linaria tingitana (Scrophulariaceae) sur le dysfonctionnement mitochondrial et lysosomal hépatocytaire induit par l’acide valproïque chez le rat. [texte imprimé] / Mourad Hanfer, Auteur ; Souad Ameddah, Directeur de thèse ; Didier Morin, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 202 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
BiologieTags : Valproïc acid Linaria tingitana LC-MS. Mitochondrial dysfunction Lysosomal dysfunction Rats HepG2 Inflammation Oxidative stress Acide valproïque LC-MS. Dysfonctionnement mitochondrial Dysfonctionnement lysosomal Stress oxydant Index. décimale : 570 Sciences de la vie. Biologie Résumé : This investigation is interested to study the modulator effect of Linaria tingitana in rats
treated by the valproic acid (VPA). The in vitro studies (DPPH◦, FRAP, β-carotene, Chelation, NO◦) showed that Linaria tingitana is endowed with an antioxidant property. The antiinflammatory studies (albumin denaturation, HRBC, NO scavenger, oedema and peritonitis led
by the carrageenan, the rise of MPO and the infiltration of the PN) confirmed that Linaria
tingitana has a beneficial effect in this sense. The in vivo studies made to rats handled by the
VPA revealed that the n-BuOH extract acts by a multitude of mechanisms: conservation of the
hepatic function, the modulation of the steatoses, the neutralization of the oxidative stress and
the reduction of the inflammation hepatocyte elucidated by the decrease of the activity MPO.
Antirrhides molecules isolated of this plant were ineffective at the level of the cell line HepG2
treated with the VPA. The mitochondrial dysfunction led by the VPA is expressed by the decrease of the RCR accompanied by a decrease of the activity of the complex I. The n-BuOH
extract as well as the isolated molecules managed to reduce the mitochondrial H2O2 and to modulate the antioxidant mitochondrial and lysosomal systems. The conservation of the integrity
lysosomal membrane was more pronounced by the n-BuOH extract compared to the vitamin
E. The various modulator ways of L. tingitana delayed the appearance of the complications
linked to the mitochondrial dysfunction, lysosomal, such necrosis. Probably this is due to the
diversified wealth of this plant in term of polyphenols and irridoids revealed by phytochemical
screening and the analysis of the LC-MS and in the synergetic effect of the molecules that it
contains.Diplôme : Doctorat En ligne : ../theses/biologie/HAN7131.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10471 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAN/7131 HAN/7131 Thèse Bibliothèque principale Thèses Disponible 
Titre : Rhantherium والمستخلص البوتانولي لنباتي C ،E دور فیتامین في الوقایة من التسمم Chrysanthemum fontanesii و suaveolens لدى الفئران الحوامل Sodium Valproate المحرض بدواء : دراسةIn vitro و In vivo Type de document : texte imprimé Auteurs : أمال عمراني, Auteur ; جميلة زعمة, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2013 Importance : 140 ورقة. Format : 30 سم. Note générale : 2نسخ موجودة في المكتبة المركزية Langues : Arabe (ara) Catégories : Arabe
5 البيولوجياTags : Valproic acid embryotoxicity Oxidative stress, Lipid peroxidation, Plant extract, Flavonoids,
Vitamin E Vitamin C Chrysanthemum fontanesii Rhantherium suaveolens Antioxidant activity Phenolic
compounds DPPH Membrane stabilizing activity Antibacterial activity Anticancer activity.Index. décimale : 570 بيولوجيا Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AAME3933.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9390 Rhantherium والمستخلص البوتانولي لنباتي C ،E دور فیتامین في الوقایة من التسمم Chrysanthemum fontanesii و suaveolens لدى الفئران الحوامل Sodium Valproate المحرض بدواء : دراسةIn vitro و In vivo [texte imprimé] / أمال عمراني, Auteur ; جميلة زعمة, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2013 . - 140 ورقة. ; 30 سم.
2نسخ موجودة في المكتبة المركزية
Langues : Arabe (ara)
Catégories : Arabe
5 البيولوجياTags : Valproic acid embryotoxicity Oxidative stress, Lipid peroxidation, Plant extract, Flavonoids,
Vitamin E Vitamin C Chrysanthemum fontanesii Rhantherium suaveolens Antioxidant activity Phenolic
compounds DPPH Membrane stabilizing activity Antibacterial activity Anticancer activity.Index. décimale : 570 بيولوجيا Diplôme : Doctorat en sciences En ligne : ../theses/biologie/AAME3933.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9390 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité عمر/3933 عمر/3933 Thèse Bibliothèque principale Thèses Disponible Documents numériques
AAME3933.pdfAdobe Acrobat PDF
