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Auteur Samira Benyahia |
Documents disponibles écrits par cet auteur (3)
Affiner la recherche Interroger des sources externesContribution phytochimique à la valorisation de deux plantes médicinales / Manel Boumaraf Ep Djendli
Titre : Contribution phytochimique à la valorisation de deux plantes médicinales : Echinops spinosus et Pulicaria undulata. Recherche et détermination structurale de leurs principales substances bioactives. Type de document : texte imprimé Auteurs : Manel Boumaraf Ep Djendli, Auteur ; Samira Benyahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2016 Importance : 176 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Echinops spinosus Pulicaria undulata Asteraceae huile essentielle flavonoides glycosylé sesquiterpène lactone asteriscunolide activité antioxydante activité antiinflamatoire Pulicaria undulata L. essential oil flavonoids glycosids sesquiterpens lactons antioxidant activity anti-inflammatory activity العائلة المركبة اليت الأساسية فلافونيدات غليكوزيدية سيسكيتيربينات لاكتونية- فعالية مضادة للأكسدة فعالية مضادة للالتهاب Index. décimale : 540 Chimie et sciences connexes Résumé :
In this thesis, aerial parts of two plants belong to Asteraceae family were studied. The first is Echinops spinosus, species distributed around the world and is commonly used in the treatment of various diseases. The second one is Pulicaria undulata, species present in desert areas of North Africa and all of the Middle East regions and mainly used in the treatment of various diseases.
For experimental purposes, the Echinops spinosus sample plants were collected from the
north-eastern part of Algeria in a region near Annaba, whereas the Pulicaria undulate, was harvested in the Djanet region (Tassili ) in the southeastern part of Algeria.
During the laboratory work, different extraction and separation methods were used as
maceration, chromatographic column, TLC and HPLC preparations. Additionally, and in
order to determine the structures of the isolated compounds, deferent spectroscopic methods were combined: nuclear magnetic resonance and its different applications (1H-NMR, 13C NMR, DEPT, COSY, HSQC, HMBC, NOESY and NOE difference) as well as spectrometry of mass high resolution, UV, IR and chiroptical spectroscopy (Polarimetry, Electronic Circular Dichroïsm ECD).
Seven phenolics compounds from Echinops spinosus were isolated and identified: One
aglycone flavonoid, five glycosids flavonoids and one quinic acid. For the first time, ten sesquiterpenes were obtained from the chloroformic extract of Pulicaria undulata (nine sesquiterpenes lactones belong to the Asteriscunolide type and one humulene)
The biological study includes the antioxidant activity of CHCl3, AcOEt and n-BuOH extracts for Echnops spinosus L.and the anti-inflammatory activity of the CHCl3 extract for Pulicaria undulata L., and that of two isolated compounds.
Analysis GC and GC/MS of the essential oil of Pulicaria undulata L. identified 31
compounds. The main components are carvotanacetone (14,8%), δ-cadinene (8,2%),
α-cadinol (4,7%), epi-α-cadinol (3,4%) and thujanol (4,7%).
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BOU6999.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10394 Contribution phytochimique à la valorisation de deux plantes médicinales : Echinops spinosus et Pulicaria undulata. Recherche et détermination structurale de leurs principales substances bioactives. [texte imprimé] / Manel Boumaraf Ep Djendli, Auteur ; Samira Benyahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2016 . - 176 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Echinops spinosus Pulicaria undulata Asteraceae huile essentielle flavonoides glycosylé sesquiterpène lactone asteriscunolide activité antioxydante activité antiinflamatoire Pulicaria undulata L. essential oil flavonoids glycosids sesquiterpens lactons antioxidant activity anti-inflammatory activity العائلة المركبة اليت الأساسية فلافونيدات غليكوزيدية سيسكيتيربينات لاكتونية- فعالية مضادة للأكسدة فعالية مضادة للالتهاب Index. décimale : 540 Chimie et sciences connexes Résumé :
In this thesis, aerial parts of two plants belong to Asteraceae family were studied. The first is Echinops spinosus, species distributed around the world and is commonly used in the treatment of various diseases. The second one is Pulicaria undulata, species present in desert areas of North Africa and all of the Middle East regions and mainly used in the treatment of various diseases.
For experimental purposes, the Echinops spinosus sample plants were collected from the
north-eastern part of Algeria in a region near Annaba, whereas the Pulicaria undulate, was harvested in the Djanet region (Tassili ) in the southeastern part of Algeria.
During the laboratory work, different extraction and separation methods were used as
maceration, chromatographic column, TLC and HPLC preparations. Additionally, and in
order to determine the structures of the isolated compounds, deferent spectroscopic methods were combined: nuclear magnetic resonance and its different applications (1H-NMR, 13C NMR, DEPT, COSY, HSQC, HMBC, NOESY and NOE difference) as well as spectrometry of mass high resolution, UV, IR and chiroptical spectroscopy (Polarimetry, Electronic Circular Dichroïsm ECD).
Seven phenolics compounds from Echinops spinosus were isolated and identified: One
aglycone flavonoid, five glycosids flavonoids and one quinic acid. For the first time, ten sesquiterpenes were obtained from the chloroformic extract of Pulicaria undulata (nine sesquiterpenes lactones belong to the Asteriscunolide type and one humulene)
The biological study includes the antioxidant activity of CHCl3, AcOEt and n-BuOH extracts for Echnops spinosus L.and the anti-inflammatory activity of the CHCl3 extract for Pulicaria undulata L., and that of two isolated compounds.
Analysis GC and GC/MS of the essential oil of Pulicaria undulata L. identified 31
compounds. The main components are carvotanacetone (14,8%), δ-cadinene (8,2%),
α-cadinol (4,7%), epi-α-cadinol (3,4%) and thujanol (4,7%).
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/BOU6999.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10394 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6999 BOU/6999 Thèse Bibliothèque principale Thèses Disponible
Titre : Métabolites secondaires et principes actifs : Détermination structurale de ces principaux composés naturels issus d’espèces endémiques de la région de Tébessa et de la Kabylie. Type de document : texte imprimé Auteurs : Redouane Lemoui, Auteur ; Samira Benyahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2018 Importance : 124 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Chimie organique: Phytochimie la Berberis hispanica la Stachys brachyclada flavonoïdes activité
antibactérienne l’activité antiproliférativeIndex. décimale : 540 Chimie et sciences connexes Résumé :
The present work focuses on a phytochemical and a biological study of two endemic Algerian species, Berberis hispanica from the family Berberidaceae and Stachys brachyclada from the family Lamiaceae. The phytochemical study of the ethyl acetate and n-butanol extracts of the aerial parts of the Berberis hispanica species has led to the isolation and identification of a phenolic acid, the first isolated caffeic acid in this species and two flavonoids: Tamarixetin; Rutin.The structural identification has been performed using spectroscopic analysis techniques (MS,1H NMR, 13C NMR), supported by a comparison with literature data. As for the biological study of AcOEt extracts, n-BuOH and flavonoid tamarixetin of this species, it has given interesting results. The n-BuOH extract has shown a better richness in polyphenols and flavonoids compared to the AcOET extract. The two extracts; AcOEt and n-BuOH have demonstrated a very good antibacterial activity with respect to the Klebsiella pneumonia strain (15 mm to 100 mg/ml). As for the antioxidant activity of these two extracts, it has been evaluated by the free radical scavenging method (DPPH) and has shown that the AcOEt extract (IC50 = 79,8μg/mL) has a better activity compared with the n-BuOH extract (IC50 = 136, 2 μg/ml). In addition, the antiproliferative activity of the flavonoid tamarixetin indicates a good response against HeLa cells (carcinoma of the human uterine cervix). Concerning the phytochemical study of the MeOH extract of the aerial parts of the second Stachysbrachyclada species, it has led to the separation and purification of five flavonoids isolated for the first time in the species, namely: Stachysetin ; Lutéoline-7-O- -D-allopyranosyl-(1→2)-glucopyranoside ; Isoscutellareine-7-O- -D-allopyranosyl-(1→2) glucopyranoside ; Apigénine ; Lutéoline.Their identification has been carried out by combining our spectroscopic data (MS, 1D and 2D NMR) with those of the literature.Note de contenu :
Annexe.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LEM7464.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11295 Métabolites secondaires et principes actifs : Détermination structurale de ces principaux composés naturels issus d’espèces endémiques de la région de Tébessa et de la Kabylie. [texte imprimé] / Redouane Lemoui, Auteur ; Samira Benyahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2018 . - 124 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Chimie organique: Phytochimie la Berberis hispanica la Stachys brachyclada flavonoïdes activité
antibactérienne l’activité antiproliférativeIndex. décimale : 540 Chimie et sciences connexes Résumé :
The present work focuses on a phytochemical and a biological study of two endemic Algerian species, Berberis hispanica from the family Berberidaceae and Stachys brachyclada from the family Lamiaceae. The phytochemical study of the ethyl acetate and n-butanol extracts of the aerial parts of the Berberis hispanica species has led to the isolation and identification of a phenolic acid, the first isolated caffeic acid in this species and two flavonoids: Tamarixetin; Rutin.The structural identification has been performed using spectroscopic analysis techniques (MS,1H NMR, 13C NMR), supported by a comparison with literature data. As for the biological study of AcOEt extracts, n-BuOH and flavonoid tamarixetin of this species, it has given interesting results. The n-BuOH extract has shown a better richness in polyphenols and flavonoids compared to the AcOET extract. The two extracts; AcOEt and n-BuOH have demonstrated a very good antibacterial activity with respect to the Klebsiella pneumonia strain (15 mm to 100 mg/ml). As for the antioxidant activity of these two extracts, it has been evaluated by the free radical scavenging method (DPPH) and has shown that the AcOEt extract (IC50 = 79,8μg/mL) has a better activity compared with the n-BuOH extract (IC50 = 136, 2 μg/ml). In addition, the antiproliferative activity of the flavonoid tamarixetin indicates a good response against HeLa cells (carcinoma of the human uterine cervix). Concerning the phytochemical study of the MeOH extract of the aerial parts of the second Stachysbrachyclada species, it has led to the separation and purification of five flavonoids isolated for the first time in the species, namely: Stachysetin ; Lutéoline-7-O- -D-allopyranosyl-(1→2)-glucopyranoside ; Isoscutellareine-7-O- -D-allopyranosyl-(1→2) glucopyranoside ; Apigénine ; Lutéoline.Their identification has been carried out by combining our spectroscopic data (MS, 1D and 2D NMR) with those of the literature.Note de contenu :
Annexe.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LEM7464.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=11295 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LEM/7464 LEM/7464 Thèse Bibliothèque principale Thèses Disponible
Titre : Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) Type de document : texte imprimé Auteurs : Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 353 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
ChimieTags : Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum تقنیات النموذجیة الجزیئیة مضاد للاكسدة مضاد لليشمانيا مضاد الملاريا مضاد التريبانوسوما" Index. décimale : 540 Chimie et sciences connexes Résumé : Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LAR7157.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10430 Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) [texte imprimé] / Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 353 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
ChimieTags : Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum تقنیات النموذجیة الجزیئیة مضاد للاكسدة مضاد لليشمانيا مضاد الملاريا مضاد التريبانوسوما" Index. décimale : 540 Chimie et sciences connexes Résumé : Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LAR7157.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10430 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAR/7157 LAR/7157 Thèse Bibliothèque principale Thèses Disponible
