| Titre : |
Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) |
| Type de document : |
texte imprimé |
| Auteurs : |
Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse |
| Editeur : |
جامعة الإخوة منتوري قسنطينة |
| Année de publication : |
2017 |
| Importance : |
353 f. |
| Format : |
30 cm. |
| Note générale : |
2 copies imprimées disponibles
|
| Langues : |
Anglais (eng) |
| Catégories : |
Français - Anglais
Chimie
|
| Tags : |
Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum ?????? ????????? ???????? ???? ??????? ???? ????????? ???? ???????? ???? ?????????????" |
| Index. décimale : |
540 Chimie et sciences connexes |
| Résumé : |
Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/chimie/LAR7157.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10430 |
Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) [texte imprimé] / Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse . - [S.l.] : جامعة الإخوة منتوري قسنطينة, 2017 . - 353 f. ; 30 cm. 2 copies imprimées disponibles
Langues : Anglais ( eng)
| Catégories : |
Français - Anglais
Chimie
|
| Tags : |
Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum ?????? ????????? ???????? ???? ??????? ???? ????????? ???? ???????? ???? ?????????????" |
| Index. décimale : |
540 Chimie et sciences connexes |
| Résumé : |
Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
|
| Diplôme : |
Doctorat en sciences |
| En ligne : |
../theses/chimie/LAR7157.pdf |
| Format de la ressource électronique : |
pdf |
| Permalink : |
https://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=10430 |
|
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