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Caractérisation chimique des principes à effet antidermatophyte des racines d'anacyclus L. / Souad Hamimed ép Benmabrouk
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Titre : Caractérisation chimique des principes à effet antidermatophyte des racines d'anacyclus L. Type de document : texte imprimé Auteurs : Souad Hamimed ép Benmabrouk, Auteur ; Univ. de Constantine, Éditeur scientifique Editeur : Constantine : Université Mentouri Constantine Année de publication : 2009 Importance : 138 f. Format : 30 cm. Note générale : 01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : chimie organique:Substances thérapeutique d’origine naturelle Anacyclus pyrethrum Racines Asteraceae Criblage phytochimique Bioactivités antifongiques Triterpènes pentacycliques et coumarines roots phytochemical screening bio guided isolation antifungal pentacyclic triterpenes and coumarins mالقنطس الإفريقي( جذور Asteraceaeدراسة فيتو آيميائية دراسة بيوموجهة ضد الفطريات التربيناتالثلاثية ذات النوع الخماسى و الكومارينا ت Index. décimale : 540 Chimie et sciences connexes Résumé : A preliminary biological evaluation of Anacyclus pyrethrum roots (Asteraceae) for antifungal effect
localized a potent activity in the dichloromethane (DCM) extract. Bio guided fractionation of DCM active
extract able us to track down a major activity in enriched fractions of triterpenes and alkamides. From the
triterpene fraction, four compounds were isolated, from which two were formally identified as pentacyclic
triterpenes. From the crude alcoholic extract of the roots, also investigated in this work, four products
were isolated, one of which is a pyranocoumarin, and the other three compounds were identified as a
phenylpropane derivatives. The nature of these phenylpropane, probably of lignan type, is under
investigation.
From the chemical point of view, the results obtained in this work provide a better understanding of
secondary metabolism in the roots of A. pyrethrum. The metabolic profile of the roots is characterized by
the presence of pentacyclic triterpenes, phenylpropane derivatives, coumarins and alkamides. Besides the
already reported alkamides, the other types of secondary metabolites are reported for the first time in this
species.
From the biological point of view, the results obtained by bioguided fractionation allowed to track a
significant antifungal activity in the moderately polar fractions, mainly containing triterpenes and
alkamides compounds.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/chimie/HAM5430.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=3795 Caractérisation chimique des principes à effet antidermatophyte des racines d'anacyclus L. [texte imprimé] / Souad Hamimed ép Benmabrouk, Auteur ; Univ. de Constantine, Éditeur scientifique . - Constantine : Université Mentouri Constantine, 2009 . - 138 f. ; 30 cm.
01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C. 01 CD
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : chimie organique:Substances thérapeutique d’origine naturelle Anacyclus pyrethrum Racines Asteraceae Criblage phytochimique Bioactivités antifongiques Triterpènes pentacycliques et coumarines roots phytochemical screening bio guided isolation antifungal pentacyclic triterpenes and coumarins mالقنطس الإفريقي( جذور Asteraceaeدراسة فيتو آيميائية دراسة بيوموجهة ضد الفطريات التربيناتالثلاثية ذات النوع الخماسى و الكومارينا ت Index. décimale : 540 Chimie et sciences connexes Résumé : A preliminary biological evaluation of Anacyclus pyrethrum roots (Asteraceae) for antifungal effect
localized a potent activity in the dichloromethane (DCM) extract. Bio guided fractionation of DCM active
extract able us to track down a major activity in enriched fractions of triterpenes and alkamides. From the
triterpene fraction, four compounds were isolated, from which two were formally identified as pentacyclic
triterpenes. From the crude alcoholic extract of the roots, also investigated in this work, four products
were isolated, one of which is a pyranocoumarin, and the other three compounds were identified as a
phenylpropane derivatives. The nature of these phenylpropane, probably of lignan type, is under
investigation.
From the chemical point of view, the results obtained in this work provide a better understanding of
secondary metabolism in the roots of A. pyrethrum. The metabolic profile of the roots is characterized by
the presence of pentacyclic triterpenes, phenylpropane derivatives, coumarins and alkamides. Besides the
already reported alkamides, the other types of secondary metabolites are reported for the first time in this
species.
From the biological point of view, the results obtained by bioguided fractionation allowed to track a
significant antifungal activity in the moderately polar fractions, mainly containing triterpenes and
alkamides compounds.Note de contenu : Annexes. Diplôme : Magistère En ligne : ../theses/chimie/HAM5430.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=3795 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité HAM/5430 HAM/5430 Thèse Bibliothèque principale Thèses Disponible Etude des activités biologiques de l’huile essentielle extraite des graines de foeniculum vulgare Mill / Ali Bouguerra
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Titre : Etude des activités biologiques de l’huile essentielle extraite des graines de foeniculum vulgare Mill : en vue de son utilisation comme conservateur alimentaire. Type de document : texte imprimé Auteurs : Ali Bouguerra, Auteur ; Malika Barkat, Directeur de thèse Editeur : Constantine : Université Mentouri Constantine Année de publication : 2012 Importance : 111 f. Format : 31 cm. Langues : Français (fre) Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Biotechnologie alimentaire: Sciences Alimentaires Huile essentielle activité antioxydante activité antibactérienne activité antifongique Foeniculum vulgare Mill
Essential oil antioxidant activity antibacterial activity antifungal Feoniculum vulgare
Mill الزیوت الأساسیة الفاعلیة المضادة للأكسدة الفاعلیة المضادة للبكتیریا الفاعلیة المضادة للفطریات (Foeniculum vulgare Mill.) ذرة الشمرةIndex. décimale : 640 Industrie Alimentaire Résumé :
The synthetic food conservatives are limited in several countries, because of their undesirable effects on health. Moreover, the current trend of the consumers seeks a more natural food increased during these last decades. Several research tasks have concentrated on the essential oils extracted from the aromatic plants. The various results published have indicated that they are endowed with several biological properties. In this context, we tried to evaluate antioxidant, antibacterial and
antifungal activities of essential oil extracted from Foeniculum vulgare Mill. seeds.
The extraction was carried out by water distillation Clevenger’s type apparatus, the yield was 0.79±0.02%. The evaluation of the antiradical activity of the fennel seeds essential oil was evaluated by the reduction of the DPPH• method. The EC50 (efficiencies’ concentration) obtained is 752.65±32.5µg.ml-1. The fennel seeds essential oil shows also a very interesting antioxidant activity (81.74± 3.92%) by the of β-Carotene bleaching method. Diffusion Method has demonstrated that the fennel seeds essential oil has a feeble antibacterial activity. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) are estimated by agar dilution method, the MIC obtained are included between 20 000 and 45 000 µg.ml-1, the MBC proved also raised. The antifungal activities of the essential oil against fungi were undertaken using inverted petriplate. The results obtained indicate that the fennel seeds essential oil has strong activity. Minimum fongistatic concentrations (MFCS), and minimum fungicidal concentration (MFCC) were estimated by broth dilution method, the MFCS obtained are included between 625 and 1250µg.ml-1, the MFCC obtained is 1250µg.ml-1. The antifungal index (AI50) were estimated, the Alternaria was
most sensitive where his AI50 was 26.22±0,693µg.ml-1. With the rise of this study, we can conclude that the fennel seeds essential oil could be regarded as a very promising preservative for food industry able to prevent the oxidation of food and to reduce growth in of deterioration of food and producing mycotoxins mycelial.Note de contenu :
Annexes.Diplôme : Magistère En ligne : ../theses/agronomie/BOU6092.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5996 Etude des activités biologiques de l’huile essentielle extraite des graines de foeniculum vulgare Mill : en vue de son utilisation comme conservateur alimentaire. [texte imprimé] / Ali Bouguerra, Auteur ; Malika Barkat, Directeur de thèse . - Constantine : Université Mentouri Constantine, 2012 . - 111 f. ; 31 cm.
Langues : Français (fre)
Catégories : Français - Anglais
Nutrition, Alimentation et Technologies Agro-AlimentairesTags : Biotechnologie alimentaire: Sciences Alimentaires Huile essentielle activité antioxydante activité antibactérienne activité antifongique Foeniculum vulgare Mill
Essential oil antioxidant activity antibacterial activity antifungal Feoniculum vulgare
Mill الزیوت الأساسیة الفاعلیة المضادة للأكسدة الفاعلیة المضادة للبكتیریا الفاعلیة المضادة للفطریات (Foeniculum vulgare Mill.) ذرة الشمرةIndex. décimale : 640 Industrie Alimentaire Résumé :
The synthetic food conservatives are limited in several countries, because of their undesirable effects on health. Moreover, the current trend of the consumers seeks a more natural food increased during these last decades. Several research tasks have concentrated on the essential oils extracted from the aromatic plants. The various results published have indicated that they are endowed with several biological properties. In this context, we tried to evaluate antioxidant, antibacterial and
antifungal activities of essential oil extracted from Foeniculum vulgare Mill. seeds.
The extraction was carried out by water distillation Clevenger’s type apparatus, the yield was 0.79±0.02%. The evaluation of the antiradical activity of the fennel seeds essential oil was evaluated by the reduction of the DPPH• method. The EC50 (efficiencies’ concentration) obtained is 752.65±32.5µg.ml-1. The fennel seeds essential oil shows also a very interesting antioxidant activity (81.74± 3.92%) by the of β-Carotene bleaching method. Diffusion Method has demonstrated that the fennel seeds essential oil has a feeble antibacterial activity. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) are estimated by agar dilution method, the MIC obtained are included between 20 000 and 45 000 µg.ml-1, the MBC proved also raised. The antifungal activities of the essential oil against fungi were undertaken using inverted petriplate. The results obtained indicate that the fennel seeds essential oil has strong activity. Minimum fongistatic concentrations (MFCS), and minimum fungicidal concentration (MFCC) were estimated by broth dilution method, the MFCS obtained are included between 625 and 1250µg.ml-1, the MFCC obtained is 1250µg.ml-1. The antifungal index (AI50) were estimated, the Alternaria was
most sensitive where his AI50 was 26.22±0,693µg.ml-1. With the rise of this study, we can conclude that the fennel seeds essential oil could be regarded as a very promising preservative for food industry able to prevent the oxidation of food and to reduce growth in of deterioration of food and producing mycotoxins mycelial.Note de contenu :
Annexes.Diplôme : Magistère En ligne : ../theses/agronomie/BOU6092.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=5996 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité BOU/6092 BOU/6092 Thèse Bibliothèque principale Thèses Disponible Etude phytochimique et valorisation biologique des deux plantes, Genista quadriflora Munby (Fabaceae) et Convolvulus tricolor L. (Convolvulaceae) / Nassira Kacem
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Titre : Etude phytochimique et valorisation biologique des deux plantes, Genista quadriflora Munby (Fabaceae) et Convolvulus tricolor L. (Convolvulaceae) Type de document : texte imprimé Auteurs : Nassira Kacem, Auteur ; Salah Rhouati, Directeur de thèse ; Kurt Hostettmann, Directeur de thèse Editeur : constantine [Algérie] : Université Constantine 1 Année de publication : 2015 Importance : 299 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Français (fre) Catégories : Français - Anglais
ChimieTags : Genista Convolvulus alcaloïdes acides caféoylquiniques sesquiterpènes flavonoïdes HPLC MPLC CPC ESIMS GC/EIMS HR-MS/TOF Q-TRAP MSn RMN-2D leishmanicide antipaludique antibactérienne antifongique antiradicalaire acétylcholinestérase alkaloids caffeoylquinic acids sesquiterpenoids flavonoids antileishmanial antimalarial antibacterial antifungal antiradical acetylcholinesterase قلويدات احماض كافيولكينية سيسكويتربنات فلافونيدات مضاد للأكسدة
الضد ميكروبية الضد فطرية مضاد للإنزيم اسيتلكولنستغاز مضاد للملاريا مضاد للشمانيا،Index. décimale : 540 Chimie et sciences connexes Résumé : This work was focused on two Algerian plants, the endemic species Genista quadriflora Munby (Fabaceae) and the species Convolvulus tricolor (Convolvulaceae). The study of the alkaloid profile of G. quadriflora by GC/EIMS allowed the identification of twenty compounds, among which ten are newly registered for the genus. We indicate the presence of:
• Thirteen quinolizidine alkaloids
• Five piperidine alkaloids
• One nicotinic alkaloid, in addition to a nicotinic acid derivative.
The investigation of the G. quadriflora essential oil has lead to the identification of 222 compounds,of which 197 are reported for the first time in the genus. This oil is mainly composed of terpenoids(66.85%) with a prevalence of sesquiterpenoids (65.18%). The major compounds are cited as:
• Salvial-4 (14) - in-1-one; germacra-4 (15), 5, 10 (14) - trien-1-α-ol; amorpha-4, 9-dien-2-
ol; muurolan-4, 7-peroxide.
Also, twenty two compounds (caffeoylquinic acids, flavonoids, coumarin, phenol acid) were isolated from C. tricolor and G. quadriflora. All these compounds are reported for the first time in the studied species. The structural determination was carried out by mass spectrometry in electronic impact EIMS, by electrospray ESIMS, by high-resolution mass spectrometry HRMS, by time of flight HR-TOFMS, and by mass spectrometry high-resolution tandem MSn. In addition, we used NMR-1D and 2D spectroscopy.
The second part of our work was an in vitro evaluation of the biological activities such as:
• antileishmanial activity, antimalarial activity, antifungal and antibacterial activities, antioxidant activity against the DPPH, and the activity towards the enzyme acetylcholinesterase.
The various tests carried out during this work have leads to tracks quite interesting especially for:
• Samples of Convolvulus tricolor with inhibitions of the parasitic activity going from 89 to 99% by quite low rates of toxicity indicating their good selective activity on the parasites.
• The alkaloids extract resulting from the air parts of Genista quadriflora for which the bacteria type Pseudomonas aeruginosa had a very particular sensitivity.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/KAC6789.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9979 Etude phytochimique et valorisation biologique des deux plantes, Genista quadriflora Munby (Fabaceae) et Convolvulus tricolor L. (Convolvulaceae) [texte imprimé] / Nassira Kacem, Auteur ; Salah Rhouati, Directeur de thèse ; Kurt Hostettmann, Directeur de thèse . - constantine [Algérie] : Université Constantine 1, 2015 . - 299 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Français (fre)
Catégories : Français - Anglais
ChimieTags : Genista Convolvulus alcaloïdes acides caféoylquiniques sesquiterpènes flavonoïdes HPLC MPLC CPC ESIMS GC/EIMS HR-MS/TOF Q-TRAP MSn RMN-2D leishmanicide antipaludique antibactérienne antifongique antiradicalaire acétylcholinestérase alkaloids caffeoylquinic acids sesquiterpenoids flavonoids antileishmanial antimalarial antibacterial antifungal antiradical acetylcholinesterase قلويدات احماض كافيولكينية سيسكويتربنات فلافونيدات مضاد للأكسدة
الضد ميكروبية الضد فطرية مضاد للإنزيم اسيتلكولنستغاز مضاد للملاريا مضاد للشمانيا،Index. décimale : 540 Chimie et sciences connexes Résumé : This work was focused on two Algerian plants, the endemic species Genista quadriflora Munby (Fabaceae) and the species Convolvulus tricolor (Convolvulaceae). The study of the alkaloid profile of G. quadriflora by GC/EIMS allowed the identification of twenty compounds, among which ten are newly registered for the genus. We indicate the presence of:
• Thirteen quinolizidine alkaloids
• Five piperidine alkaloids
• One nicotinic alkaloid, in addition to a nicotinic acid derivative.
The investigation of the G. quadriflora essential oil has lead to the identification of 222 compounds,of which 197 are reported for the first time in the genus. This oil is mainly composed of terpenoids(66.85%) with a prevalence of sesquiterpenoids (65.18%). The major compounds are cited as:
• Salvial-4 (14) - in-1-one; germacra-4 (15), 5, 10 (14) - trien-1-α-ol; amorpha-4, 9-dien-2-
ol; muurolan-4, 7-peroxide.
Also, twenty two compounds (caffeoylquinic acids, flavonoids, coumarin, phenol acid) were isolated from C. tricolor and G. quadriflora. All these compounds are reported for the first time in the studied species. The structural determination was carried out by mass spectrometry in electronic impact EIMS, by electrospray ESIMS, by high-resolution mass spectrometry HRMS, by time of flight HR-TOFMS, and by mass spectrometry high-resolution tandem MSn. In addition, we used NMR-1D and 2D spectroscopy.
The second part of our work was an in vitro evaluation of the biological activities such as:
• antileishmanial activity, antimalarial activity, antifungal and antibacterial activities, antioxidant activity against the DPPH, and the activity towards the enzyme acetylcholinesterase.
The various tests carried out during this work have leads to tracks quite interesting especially for:
• Samples of Convolvulus tricolor with inhibitions of the parasitic activity going from 89 to 99% by quite low rates of toxicity indicating their good selective activity on the parasites.
• The alkaloids extract resulting from the air parts of Genista quadriflora for which the bacteria type Pseudomonas aeruginosa had a very particular sensitivity.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/KAC6789.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=9979 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité KAC/6789 KAC/6789 Thèse Bibliothèque principale Thèses Disponible
Titre : Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) Type de document : texte imprimé Auteurs : Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse Editeur : جامعة الإخوة منتوري قسنطينة Année de publication : 2017 Importance : 353 f. Format : 30 cm. Note générale : 2 copies imprimées disponibles
Langues : Anglais (eng) Catégories : Français - Anglais
ChimieTags : Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum تقنیات النموذجیة الجزیئیة مضاد للاكسدة مضاد لليشمانيا مضاد الملاريا مضاد التريبانوسوما" Index. décimale : 540 Chimie et sciences connexes Résumé : Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LAR7157.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10430 Phytochemical and biological studies of two Algerian medicinal plants : Cytisus villosus Pourr. (Fabaceae) and Hypericum afrum Lam. (Hypericaceae) [texte imprimé] / Farida Larit, Auteur ; Samira Benyahia, Directeur de thèse . - جامعة الإخوة منتوري قسنطينة, 2017 . - 353 f. ; 30 cm.
2 copies imprimées disponibles
Langues : Anglais (eng)
Catégories : Français - Anglais
ChimieTags : Hypericum Cytisus Secondary metabolites Total phenolics Antioxidant Cytotoxicity Antifungal Antibacterial Antimalarial Antileishmanial Activity MAO-A and MAO-B Docking Simulation Métabolites secondaires Total phénoliques Cytotoxicité Antifongique Antibactérienne Antipaludique Antileishmanienne MAO-A et MAO-B "MAO-A MAO-B Cytisus Hypericum تقنیات النموذجیة الجزیئیة مضاد للاكسدة مضاد لليشمانيا مضاد الملاريا مضاد التريبانوسوما" Index. décimale : 540 Chimie et sciences connexes Résumé : Natural products are important sources of novel therapeutic agents. Bioactive secondary metabolites
isolated from natural sources, can act as drugs or as lead compounds for synthetic drugs. In our work, two plants
have been studied; the endemic species of Hypericum afrum Lam.(Hypericaceae) and Cytisus villosus Pourr.
(Fabaceae). Fractions of the aerial parts of these two plants have been screened in vitro for several biological
assays including cannabinoid and opioid receptors agonist assays, antifungal, antibacterial, antimalarial,
antileishmanial, antiproliferative, antioxidant, anti-inflammatory and MAO inhibition.
The chloroform, ethyl acetate and n-butanol fractions of H. afrum showed significant antioxidant activity and
potent antitrypanosomal activity against T. brucei. The n-butanol fraction of C. villosus showed highly potent
antitrypanosomal activity against T. brucei. In addition, the ethyl acetate fractions of both plants showed potent
inhibition of recombinant human monoamine oxidases (MAO-A and -B).
A bioassay-guided fractionation paradigm has been used for the isolation of bioactive compounds of these plants
and hence the subfractions that showed significant activity were further purified.
This study led to the isolation and identification of 20 compounds, three of which belonging to phloroglucinols,
terpenoids and isoflavonoids are new. The structures of the isolated compounds were elucidated through various
spectroscopic methods, including high- resolution mass spectrometry, one and two-dimensional nuclear magnetic
resonance spectroscopy.
Computational study was carried out by conformational search and docking techniques to provide insight into the
binding mode of molecules on the active site of MAO’s isoenzymes.
Diplôme : Doctorat en sciences En ligne : ../theses/chimie/LAR7157.pdf Format de la ressource électronique : Permalink : index.php?lvl=notice_display&id=10430 Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité LAR/7157 LAR/7157 Thèse Bibliothèque principale Thèses Disponible